FastQCFastQC Report
Wed 25 May 2016
SRR1780570_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780570_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences156809
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGT13230.8437015732515353No Hit
ATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGTCTT5770.36796357351937703RNA PCR Primer, Index 48 (95% over 23bp)
ATAAAGAATATTGAGGCGCCATTGGCGTGAAGGTAGCGGATGATTCAGCC4360.27804526525900936No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCC1840.11734020368728834No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACAGGT150.002221313370.035
ATCAGAA150.002221313370.042
AAGGTTC150.002221313370.07
TTCAACG150.002221313370.019
CACTGAC150.002221313370.054
AACGATT150.002221313370.022
CGATCCT150.002221313370.064
AATTTAC700.060.00000453
TCTCGAG700.060.00000461
ACGTCTC700.060.00000458
CTCGAGT700.060.00000462
TGGCGTG950.058.94736523
GCGTGAA900.058.33333625
CGAGTGA750.056.064
GTCTCGA750.056.060
GAAGCTC252.3846529E-455.99999633
CTCGTGG252.3846529E-455.9999968
CCTCGTG252.3846529E-455.9999967
ATAAAGA1000.055.9999961
TAAAGAA1050.053.3333322
Produced by FastQC (version 0.11.5)