FastQCFastQC Report
Wed 25 May 2016
SRR1780568_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780568_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51351
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATACCT150.002213519369.99415628
TTATGAC150.002213519369.99415650
CCGTGCC150.002213519369.99415648
TACGGAC150.002213519369.99415638
TCCGTGC150.002213519369.99415647
TCTCCGT150.002213519369.99415645
TTCTATA200.00691674852.495622
CGTGGTA200.00691674852.495628
TGGAGAG200.00691674852.4956244
GTTTCAT200.00691674852.4956239
ATCAGCC200.00691674852.4956219
GAATCGA200.00691674852.4956215
CAGATCA200.00691674852.4956214
CTAAAGC200.00691674852.4956260
ATGACTC200.00691674852.4956252
TTAAAAT200.00691674852.4956221
GAACCCA200.00691674852.4956220
GCTACAT200.00691674852.4956229
CTTTACG200.00691674852.4956235
AAGATAC200.00691674852.4956226
Produced by FastQC (version 0.11.5)