##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780568_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 51351 Sequences flagged as poor quality 0 Sequence length 76 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.19504975560359 34.0 27.0 34.0 12.0 34.0 2 30.40928122139783 34.0 31.0 34.0 21.0 34.0 3 30.768728943934878 34.0 32.0 34.0 21.0 34.0 4 31.305427352923992 34.0 33.0 34.0 23.0 34.0 5 31.10492492843372 34.0 33.0 34.0 23.0 34.0 6 33.2090903779868 37.0 34.0 38.0 21.0 38.0 7 32.568596521976204 37.0 32.0 38.0 11.0 38.0 8 31.273801873381238 36.0 27.0 38.0 11.0 38.0 9 32.363751436194036 37.0 31.0 38.0 12.0 38.0 10-11 32.8076571050223 37.0 32.5 38.0 16.0 38.0 12-13 33.289166715351215 37.0 33.5 38.0 21.0 38.0 14-15 32.86830830947791 37.0 32.5 38.0 16.0 38.0 16-17 32.73797978617749 37.0 32.0 38.0 11.5 38.0 18-19 32.71192381842613 37.0 32.0 38.0 16.0 38.0 20-21 32.772721076512624 37.0 32.0 38.0 16.0 38.0 22-23 32.79167883780258 37.0 32.5 38.0 16.0 38.0 24-25 33.31834823080368 37.0 34.0 38.0 21.0 38.0 26-27 33.413477829058834 37.0 34.0 38.0 21.0 38.0 28-29 33.60137095674865 37.0 34.0 38.0 21.0 38.0 30-31 33.42787871706491 37.0 34.0 38.0 21.0 38.0 32-33 33.17781542715819 37.0 34.0 38.0 21.0 38.0 34-35 32.95949445969893 37.0 33.5 38.0 21.0 38.0 36-37 32.955531537847364 37.0 33.0 38.0 16.0 38.0 38-39 33.565198340830754 37.0 34.0 38.0 21.0 38.0 40-41 32.98493700220054 37.0 33.0 38.0 21.0 38.0 42-43 33.355367957780764 37.0 34.0 38.0 21.0 38.0 44-45 32.27297423613951 37.0 31.0 38.0 11.0 38.0 46-47 33.06486728593406 37.0 33.5 38.0 21.0 38.0 48-49 32.73679188331289 37.0 31.5 38.0 16.0 38.0 50-51 32.62805008665849 37.0 31.5 38.0 16.0 38.0 52-53 32.61075733676073 37.0 31.5 38.0 15.0 38.0 54-55 32.259917041537655 37.0 31.0 38.0 11.0 38.0 56-57 32.41204650347608 37.0 31.5 38.0 15.0 38.0 58-59 32.28833907810948 37.0 31.5 38.0 15.0 38.0 60-61 32.27811532394695 37.0 31.0 38.0 11.0 38.0 62-63 32.36111273392923 37.0 31.0 38.0 11.0 38.0 64-65 32.37786021693833 37.0 31.0 38.0 11.0 38.0 66-67 32.33019804872349 37.0 31.0 38.0 11.0 38.0 68-69 31.8778504800296 37.0 29.0 38.0 11.0 38.0 70-71 31.780384023680163 36.5 30.5 38.0 11.0 38.0 72-73 31.866000662109794 37.0 30.0 38.0 11.0 38.0 74-75 31.458676559365934 36.0 28.0 38.0 11.0 38.0 76 30.653638682790987 35.0 27.0 37.0 11.0 38.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -2.877345619847354 1101 2 -1.644470182635402 1101 3 -0.48614214993313 1101 4 -3.053711796790431 1101 5 -1.695098238461842 1101 6 -3.514280362692215 1101 7 -1.0759520625025374 1101 8 -1.9228053994932175 1101 9 -1.5192995804666474 1101 10-11 -1.625277892109942 1101 12-13 -1.103513836625794 1101 14-15 -1.0280360521293623 1101 16-17 -2.4517010987165477 1101 18-19 -0.4401471758818758 1101 20-21 -2.0966576469714227 1101 22-23 -1.115022347905466 1101 24-25 -2.73577157082304 1101 26-27 -1.7133900378492513 1101 28-29 -0.7090294523174165 1101 30-31 -1.6875259041408697 1101 32-33 -0.6155322003665091 1101 34-35 -1.1452412241904355 1101 36-37 -0.6075484078782054 1101 38-39 -0.5844447961532495 1101 40-41 -0.2906509060491089 1101 42-43 -1.2039995284189686 1101 44-45 -0.23463767771874444 1101 46-47 0.05791968905225531 1101 48-49 -1.7570895471775358 1101 50-51 -1.5168835806254535 1101 52-53 -0.8914122554579968 1101 54-55 -1.5683737829917206 1101 56-57 -1.1250657082948265 1101 58-59 0.09115761175156933 1101 60-61 -0.5235757757004222 1101 62-63 -1.549918719010499 1101 64-65 -1.6589309321821872 1101 66-67 -1.8768118021849816 1101 68-69 0.1634681481300504 1101 70-71 0.007696712990750143 1101 72-73 -1.663670894823202 1101 74-75 -2.11812801525819 1101 76 -1.609006945087181 1102 1 0.46316610723153673 1102 2 -0.44329747474627723 1102 3 -0.34168585995445255 1102 4 0.2666506765571164 1102 5 -0.5376355518946809 1102 6 0.4294296586297506 1102 7 0.7563507093524748 1102 8 2.8488363915515613 1102 9 1.1013827223052033 1102 10-11 -0.14649324392230767 1102 12-13 -4.13107247202025 1102 14-15 -1.2011703804875715 1102 16-17 -1.955539051808234 1102 18-19 -0.9489424850503241 1102 20-21 -0.21829943801620288 1102 22-23 -0.9702462285024822 1102 24-25 0.07920710721533197 1102 26-27 -5.773304750002772 1102 28-29 -0.011694697519978092 1102 30-31 0.7534122621704213 1102 32-33 -7.023741155590386 1102 34-35 -0.9878318425273207 1102 36-37 0.12454114436059882 1102 38-39 0.3571330290066683 1102 40-41 0.5884002666587733 1102 42-43 -0.3710570977153438 1102 44-45 -5.847697379211283 1102 46-47 -0.6223574964488137 1102 48-49 -0.315031977881155 1102 50-51 -6.3565424292395285 1102 52-53 -1.3837363492746277 1102 54-55 0.8074790954730986 1102 56-57 -1.5595006763118846 1102 58-59 0.29057125780700943 1102 60-61 0.8496651624658824 1102 62-63 -4.622573303232247 1102 64-65 -1.3948584375126813 1102 66-67 -0.7373128682830625 1102 68-69 -7.915956158906198 1102 70-71 -0.8216742891414412 1102 72-73 0.040646802404939564 1102 74-75 -5.740302855343479 1102 76 -1.5157233629976297 1103 1 -0.63553519146976 1103 2 -1.0673234487722496 1103 3 -0.5786988469674412 1103 4 -0.22360906370262157 1103 5 0.06561120135207332 1103 6 -1.1653755361754463 1103 7 0.67128577428754 1103 8 0.8332519759671477 1103 9 1.0455385664610475 1103 10-11 0.6307794833504161 1103 12-13 -1.5463322122799923 1103 14-15 -0.758313237630432 1103 16-17 -1.2490455453147256 1103 18-19 -0.7395269006347434 1103 20-21 -1.0897280094447694 1103 22-23 -1.0182981765544312 1103 24-25 -0.1795591265508989 1103 26-27 -1.538889165587186 1103 28-29 0.11622738040210123 1103 30-31 -1.1089254001672302 1103 32-33 -5.5127021945514265 1103 34-35 -1.640104569800048 1103 36-37 -0.6001341803147255 1103 38-39 -0.4681916463180116 1103 40-41 0.3991145523730637 1103 42-43 -0.12690125355950244 1103 44-45 -5.739580496094398 1103 46-47 -0.055474379565694676 1103 48-49 -0.47379821164739155 1103 50-51 -0.4130359357330349 1103 52-53 -0.8097103752486525 1103 54-55 0.12468688768088754 1103 56-57 1.1320577652465573 1103 58-59 0.8220647643005208 1103 60-61 1.2438210066217295 1103 62-63 0.1855435798846301 1103 64-65 0.36747922482498296 1103 66-67 -0.46491027088046266 1103 68-69 -2.688683431633468 1103 70-71 0.7974815550144001 1103 72-73 0.5085039452620812 1103 74-75 -1.88121194625257 1103 76 -0.8955934928677607 1104 1 -3.3316304869028066 1104 2 -0.27163238486926744 1104 3 1.3955421438298394 1104 4 0.6476818969923102 1104 5 1.5275962361847917 1104 6 0.387518589566362 1104 7 2.2440044463439577 1104 8 2.1461873849290463 1104 9 0.27896077339319447 1104 10-11 0.3543109188023905 1104 12-13 -0.39665903777238043 1104 14-15 0.23459120891639884 1104 16-17 0.07298507307351088 1104 18-19 1.309926956766141 1104 20-21 1.4303760586725431 1104 22-23 0.9612107251398996 1104 24-25 1.3858059719267786 1104 26-27 1.33672363221104 1104 28-29 0.4914746496891027 1104 30-31 -0.5286596394378975 1104 32-33 0.08420586427716614 1104 34-35 1.2489467762049458 1104 36-37 1.143415316546033 1104 38-39 0.8857044575780932 1104 40-41 1.1427285542652044 1104 42-43 1.0381671217737747 1104 44-45 -2.601174200403335 1104 46-47 1.378493969965568 1104 48-49 2.1878298953449544 1104 50-51 2.4799287155097645 1104 52-53 1.396963745332748 1104 54-55 0.8830940434390371 1104 56-57 1.21935457439767 1104 58-59 1.057056593663205 1104 60-61 1.1025979912265313 1104 62-63 1.7106575387734253 1104 64-65 0.6777054224683994 1104 66-67 0.8925121080650982 1104 68-69 -3.499920208102033 1104 70-71 0.3781128442549644 1104 72-73 1.5413800095951053 1104 74-75 -0.40264911835199513 1104 76 0.7839455111745544 1105 1 -1.781128591780142 1105 2 -0.16113215578850415 1105 3 -0.414237522128758 1105 4 0.6572616379227938 1105 5 0.7997858317531197 1105 6 0.08109183293343136 1105 7 1.8507352556238104 1105 8 1.044433875828286 1105 9 1.1079415722602803 1105 10-11 0.8954205027084257 1105 12-13 -1.592460612181977 1105 14-15 0.1711566635375874 1105 16-17 1.064092574427164 1105 18-19 1.4473962391185893 1105 20-21 -0.4160981801545631 1105 22-23 -0.05295063101820574 1105 24-25 0.6907749418963789 1105 26-27 0.6621849175623709 1105 28-29 -0.5510927208802627 1105 30-31 0.4390816242548823 1105 32-33 0.9366362029001749 1105 34-35 0.6612921466910109 1105 36-37 1.031144917945511 1105 38-39 -0.038823844308701894 1105 40-41 0.7535619917261585 1105 42-43 1.5961037288794415 1105 44-45 1.6461705453170197 1105 46-47 0.8027098889959277 1105 48-49 0.192807195524054 1105 50-51 0.5624602661782632 1105 52-53 -1.4324335640095782 1105 54-55 -1.1050770590641932 1105 56-57 -0.714711817372077 1105 58-59 -0.2448510243133839 1105 60-61 0.3426121525826886 1105 62-63 0.28310953594115773 1105 64-65 -0.03654756599425468 1105 66-67 0.5731318756522441 1105 68-69 1.3070896631962299 1105 70-71 1.2736311915413978 1105 72-73 0.7625470719467167 1105 74-75 -1.2330252273111313 1105 76 -1.5204403441297067 1106 1 0.6353038985182664 1106 2 0.42899229222434343 1106 3 -0.1828712702887998 1106 4 0.5809870494041292 1106 5 0.8903786325024825 1106 6 0.7715775816287334 1106 7 0.9508289261711198 1106 8 1.0750775263033354 1106 9 2.43137259059295 1106 10-11 0.6208319840412173 1106 12-13 1.5123165857305096 1106 14-15 1.5030849386613667 1106 16-17 0.573994889427837 1106 18-19 0.6471821350104676 1106 20-21 1.1749629733313185 1106 22-23 1.31805164383794 1106 24-25 0.7077025479448196 1106 26-27 1.1084581679303582 1106 28-29 0.4273630532399011 1106 30-31 1.0531589693639418 1106 32-33 1.772712745118831 1106 34-35 1.2244528585871706 1106 36-37 1.2830517826584753 1106 38-39 1.2935058760178109 1106 40-41 1.2329544713193599 1106 42-43 1.2277777553748237 1106 44-45 2.418082762632686 1106 46-47 1.81067188551674 1106 48-49 1.7809913250570375 1106 50-51 1.0949773275993806 1106 52-53 0.5430113710901097 1106 54-55 1.3664203315419883 1106 56-57 1.7806715627965275 1106 58-59 0.9764121899245382 1106 60-61 2.096726965910669 1106 62-63 1.4356587129784941 1106 64-65 1.8449591916666535 1106 66-67 1.899439917937805 1106 68-69 2.6896669413977534 1106 70-71 2.1893946065019207 1106 72-73 2.080692395110109 1106 74-75 -0.24547002859575784 1106 76 1.1562624526052083 1107 1 0.21230168502953717 1107 2 0.08459151524462527 1107 3 0.5989419835396319 1107 4 0.19317478938696198 1107 5 1.1817911996976775 1107 6 0.35381546390727436 1107 7 0.7064417011632145 1107 8 0.7360975380483765 1107 9 0.6363235776282252 1107 10-11 0.5017779116736918 1107 12-13 0.9243642886712848 1107 14-15 -0.4107245206149628 1107 16-17 1.278810356744998 1107 18-19 1.2256480518013575 1107 20-21 0.8973056575252016 1107 22-23 0.21797033200707006 1107 24-25 0.30534450484955045 1107 26-27 0.4310628569126038 1107 28-29 -0.44531617158731507 1107 30-31 0.7438126261376752 1107 32-33 1.9928193539637533 1107 34-35 1.2850753458257245 1107 36-37 0.372630316335119 1107 38-39 1.0608636932468798 1107 40-41 1.0342373913175607 1107 42-43 0.8194342580626355 1107 44-45 0.7960924297059151 1107 46-47 1.224594277891491 1107 48-49 0.8930435453217527 1107 50-51 1.4988260875939545 1107 52-53 1.7526603750201915 1107 54-55 0.6818876487033094 1107 56-57 1.2742117895661877 1107 58-59 1.4017859984803493 1107 60-61 1.118500467288456 1107 62-63 0.973491300953377 1107 64-65 0.8907648563908737 1107 66-67 1.4970592882228502 1107 68-69 1.3208500285369311 1107 70-71 -0.18134216903225564 1107 72-73 0.6010881126870125 1107 74-75 0.8050110664035657 1107 76 0.463576000059696 1108 1 0.458278889186424 1108 2 0.937717562847709 1108 3 0.03801338816584732 1108 4 0.384131879564638 1108 5 0.21368023757900545 1108 6 0.4814973278026784 1108 7 0.8883055965705182 1108 8 0.8606784968147174 1108 9 0.136533098245053 1108 10-11 0.4049165305137805 1108 12-13 0.4453373024158367 1108 14-15 1.7106717247755867 1108 16-17 -0.7879638638383071 1108 18-19 -0.05655526700520852 1108 20-21 0.4718321409311699 1108 22-23 -0.5965620179761686 1108 24-25 -0.10885680255909591 1108 26-27 -0.37236490037871306 1108 28-29 0.4349218438334006 1108 30-31 0.8367769238245657 1108 32-33 0.5098114759885561 1108 34-35 -0.7497679327528814 1108 36-37 -0.1932220135341396 1108 38-39 0.8495202470517782 1108 40-41 0.0363325974858455 1108 42-43 1.1468940301041997 1108 44-45 0.022039462893978623 1108 46-47 0.8558379922729884 1108 48-49 1.0301747890361312 1108 50-51 1.0906380218882958 1108 52-53 1.4521659063644634 1108 54-55 0.39121969697685444 1108 56-57 1.309390301131721 1108 58-59 0.4316426863784386 1108 60-61 1.807089974495959 1108 62-63 0.6210357193241407 1108 64-65 0.8931114872993504 1108 66-67 -0.10648580061388913 1108 68-69 1.4703220365825231 1108 70-71 2.4298294702570473 1108 72-73 0.9403648475177171 1108 74-75 2.202178347664038 1108 76 0.6421713738444765 1109 1 0.13459467866010755 1109 2 -0.3004403318891349 1109 3 0.5511712829026898 1109 4 0.11307924798568791 1109 5 0.9186144481053233 1109 6 1.6972868014868894 1109 7 -0.903470719218955 1109 8 1.2113363915515585 1109 9 2.0362041508766353 1109 10-11 1.977613898934834 1109 12-13 0.43990967083689725 1109 14-15 0.35195461951242635 1109 16-17 0.05249666247748053 1109 18-19 0.9015039435211065 1109 20-21 1.9973255619838 1109 22-23 2.0578787714975135 1109 24-25 1.5751892500724765 1109 26-27 1.949016678568654 1109 28-29 1.064198159622883 1109 30-31 1.2614479764561395 1109 32-33 1.5606338444096082 1109 34-35 0.45056101461553766 1109 36-37 1.4057911443605988 1109 38-39 1.5575794575780932 1109 40-41 1.6486681238016345 1109 42-43 1.0615321879989423 1109 44-45 1.0616776207887213 1109 46-47 0.20041036069404328 1109 48-49 0.002378736404558879 1109 50-51 1.745243285046186 1109 52-53 1.7439422221539402 1109 54-55 0.5181933811873805 1109 56-57 1.2297850379738335 1109 58-59 0.38164268637844145 1109 60-61 1.8760044481801685 1109 62-63 0.5537659824820409 1109 64-65 0.8663022767730411 1109 66-67 0.41982998885979583 1109 68-69 2.4581509839509508 1109 70-71 1.348414996572842 1109 72-73 0.1089503738335118 1109 74-75 2.3717507160850957 1109 76 0.784276637002371 1110 1 -0.21171404617210499 1110 2 0.7911704063659002 1110 3 -1.1367481801845578 1110 4 -1.0495046446317602 1110 5 -0.20105837068662424 1110 6 0.23604519074863362 1110 7 0.7079806230629231 1110 8 2.2857491432294132 1110 9 -1.0344753793247072 1110 10-11 -0.5845085842866453 1110 12-13 0.1908115164744757 1110 14-15 0.0820510960224965 1110 16-17 0.3765318973768075 1110 18-19 0.7791255878164094 1110 20-21 -0.2951450756000895 1110 22-23 0.3508947110948313 1110 24-25 0.19370854537449134 1110 26-27 -1.1494313080085234 1110 28-29 0.47934077707254374 1110 30-31 0.44722817779842217 1110 32-33 1.4004199182351158 1110 34-35 1.0986734307229185 1110 36-37 0.31011161415925415 1110 38-39 0.5702682159673529 1110 40-41 0.42356325802981587 1110 42-43 0.4002276577975934 1110 44-45 1.0668999362249636 1110 46-47 0.5287450922376635 1110 48-49 1.1370054142569046 1110 50-51 -0.004337251866566305 1110 52-53 0.15010832953649356 1110 54-55 0.11483767648939391 1110 56-57 0.47525483663154944 1110 58-59 -3.861562011608136 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2116 54-55 0.28205892740587046 2116 56-57 -0.7170637015219654 2116 58-59 -0.7621598346299621 2116 60-61 -1.5919314761895755 2116 62-63 0.558729217776154 2116 64-65 0.8564020666890002 2116 66-67 -0.13584228004776833 2116 68-69 0.3298421604215349 2116 70-71 -2.933228910990181 2116 72-73 1.220688819211663 2116 74-75 -0.6873616788728896 2116 76 -1.631269581485025 2117 1 -0.35707198800655604 2117 2 -0.11710699855580131 2117 3 0.06783794956935552 2117 4 -0.4285874186809764 2117 5 -2.120968885228013 2117 6 -1.6443798651797756 2117 7 -2.9305540525522886 2117 8 -2.6844969417817737 2117 9 1.2549541508766353 2117 10-11 -0.34113610106516745 2117 12-13 0.5409513375035573 2117 14-15 -0.23033704715423653 2117 16-17 -0.4745866708558566 2117 18-19 -0.23287105647889206 2117 20-21 -0.15579943801620288 2117 22-23 0.7755871048308478 2117 24-25 0.8970642500724821 2117 26-27 0.6636000119019911 2117 28-29 0.21211482628954315 2117 30-31 0.3093646431228123 2117 32-33 1.0595921777429425 2117 34-35 -0.4640223187177952 2117 36-37 -2.342125522306066 2117 38-39 0.3148711242447604 2117 40-41 -1.7044568761983676 2117 42-43 -0.968676145334392 2117 44-45 0.6898026207887185 2117 46-47 -0.30688130597262386 2117 48-49 -0.934079596928779 2117 50-51 0.13274328504618893 2117 52-53 1.0085255554872745 2117 54-55 -1.5526399521459524 2117 56-57 -2.2202149620261693 2117 58-59 -0.96835731362156 2117 60-61 0.9353794481801714 2117 62-63 -0.7368590175179648 2117 64-65 -1.243072723226966 2117 66-67 -2.0426700111402027 2117 68-69 -0.4272656827157171 2117 70-71 0.4765399965728392 2117 72-73 0.7662420405001775 2117 74-75 -1.0897076172482407 2117 76 -0.8073900296642975 2118 1 -0.08053137176005976 2118 2 0.5747697521444799 2118 3 0.572179686264036 2118 4 0.2687515168932535 2118 5 -0.4313330308862753 2118 6 1.2016985661927748 2118 7 1.3290397849827258 2118 8 -1.3940207513055825 2118 9 -1.6977769415603383 2118 10-11 -0.9914512271155829 2118 12-13 0.8640955952066349 2118 14-15 -0.8969686998153037 2118 16-17 -0.6694045980267163 2118 18-19 -0.8113374430335156 2118 20-21 -3.423761622890151 2118 22-23 -1.505120178082315 2118 24-25 -1.6138601196754188 2118 26-27 -0.37330475000277374 2118 28-29 -0.6213585630661953 2118 30-31 -0.2932263932917607 2118 32-33 -0.26155628164080724 2118 34-35 1.1333866448676417 2118 36-37 0.18882685864631554 2118 38-39 -0.42101823149753415 2118 40-41 -2.575675363593323 2118 42-43 -1.6034100388918162 2118 44-45 -1.7760587237490952 2118 46-47 -1.426559177121085 2118 48-49 -1.3555781963685547 2118 50-51 -0.8897357065504536 2118 52-53 -1.3845766854090833 2118 54-55 -1.2641595599890891 2118 56-57 0.27453293713349325 2118 58-59 0.41010907293306076 2118 60-61 -3.402855845937477 2118 62-63 0.09654434382657229 2118 64-65 -1.4041021349916711 2118 66-67 -2.62323723803096 2118 68-69 0.7668169503375069 2118 70-71 -1.3407919362002687 2118 72-73 -2.2246893320488432 2118 74-75 -0.5024877292930583 2118 76 -0.5472359680396472 2119 1 0.10459467866010996 2119 2 0.04955966811086654 2119 3 0.5411712829026882 2119 4 -0.36192075201430995 2119 5 0.2923644481053209 2119 6 0.5622868014868914 2119 7 -1.0122207192189556 2119 8 -1.4211636084484418 2119 9 0.6899541508766376 2119 10-11 -0.7736361010651649 2119 12-13 0.19178467083689554 2119 14-15 -1.2461703804875697 2119 16-17 -1.151253337522519 2119 18-19 -1.515371056478891 2119 20-21 -2.0932994380161993 2119 22-23 0.7247537714975181 2119 24-25 0.2870642500724827 2119 26-27 -0.12973332143134542 2119 28-29 0.9454481596228774 2119 30-31 0.47769797645614176 2119 32-33 -0.37374115559039467 2119 34-35 1.0593110146155311 2119 36-37 0.09454114436059768 2119 38-39 -1.4092955424219085 2119 40-41 -0.9494568761983686 2119 42-43 -0.3353428120010591 2119 44-45 -2.197697379211281 2119 46-47 -1.5402146393059581 2119 48-49 -1.400746263595444 2119 50-51 0.6877432850461886 2119 52-53 -1.3973077778460592 2119 54-55 -2.4868066188126186 2119 56-57 -0.11021496202616987 2119 58-59 0.5891426863784375 2119 60-61 0.40537944818017024 2119 62-63 -1.276859017517964 2119 64-65 0.4944272767730382 2119 66-67 0.9848299888597936 2119 68-69 -0.5380990160490526 2119 70-71 -1.1409600034271605 2119 72-73 -2.1629246261664896 2119 74-75 -0.29387428391490644 2119 76 1.8342766370023718 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 1.0 12 11.0 13 58.0 14 216.0 15 385.0 16 523.0 17 562.0 18 569.0 19 497.0 20 562.0 21 542.0 22 566.0 23 601.0 24 751.0 25 814.0 26 976.0 27 1157.0 28 1246.0 29 1608.0 30 1965.0 31 2387.0 32 2951.0 33 3813.0 34 5068.0 35 6977.0 36 9600.0 37 6945.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 35.00774160672638 26.19602924171452 10.68733708327617 28.10889206828293 2 16.985063582013982 20.04245292204631 36.43745983525151 26.535023660688207 3 17.801016533271017 25.389963194485016 29.39767482619618 27.41134544604779 4 12.178925434752975 19.171973281922455 40.01869486475434 28.63040641857023 5 13.475881677085159 36.63998753675683 35.370294638858056 14.513836147299955 6 26.149442075129986 37.16383322622734 18.153492629160095 18.53323206948258 7 24.862222741524022 30.273996611555766 24.50195711865397 20.361823528266246 8 21.458199450838347 33.574808669743526 19.45629101672801 25.51070086269011 9 23.253685419952873 15.618001596853032 22.429942941714863 38.69837004147923 10-11 24.009269537107357 25.374384140523066 27.172791182255455 23.443555140114118 12-13 22.535101555958015 23.545792681739403 26.73073552608518 27.18837023621741 14-15 20.704562715429105 24.968355046639793 24.503904500399212 29.823177737531886 16-17 22.34425814492415 27.112422348152908 26.147494693384743 24.395824813538198 18-19 21.521489357558764 26.34904870401745 26.420128137718834 25.709333800704954 20-21 20.995696286343012 27.108527584662422 26.20981090923254 25.685965219762032 22-23 22.685049950341764 26.403575392884264 26.25070592588265 24.660668730891317 24-25 22.16802009697961 27.978033533913653 25.9887830811474 23.86516328795934 26-27 20.82237931101634 28.31395688496816 27.100738057681443 23.762925746334055 28-29 21.88759712566454 27.262370742536657 28.300325212751453 22.549706919047342 30-31 21.92946583318728 26.950789663297698 27.675215672528285 23.444528830986737 32-33 20.32969172946973 28.286693540534753 27.65574185507585 23.72787287491967 34-35 21.909992015734844 27.648926018967497 27.677163054273528 22.763918911024128 36-37 22.155362115635526 27.715136998305777 26.9420264454441 23.187474440614594 38-39 21.560436992463632 27.84950633872758 27.84366419349185 22.746392475316938 40-41 21.465988977819322 26.619734766606296 28.078323693793696 23.835952561780687 42-43 20.9324063796226 27.083211621974257 28.31493057584078 23.669451422562364 44-45 20.40758699927947 26.278942961188683 28.40840489961247 24.905065139919376 46-47 20.98206461412631 26.41720706510097 28.501879223384158 24.098849097388563 48-49 20.648088644817044 27.23510739810325 28.41814180833869 23.69866214874102 50-51 20.60719362816693 26.734630289575666 28.242877451266775 24.415298630990634 52-53 20.095032229167884 26.917684173628558 28.46293158847929 24.52435200872427 54-55 19.011314287939864 27.652820782457987 28.30616735798719 25.02969757161496 56-57 19.510817705594828 26.397733247648535 28.570037584467684 25.521411462288953 58-59 19.969426106599677 26.55352378726802 28.197113980253548 25.279936125878756 60-61 20.02103172284863 26.303285233004225 28.183482308036844 25.4922007361103 62-63 19.53515997741037 26.251679616755276 28.507721368619894 25.705439037214468 64-65 20.393955327062766 26.5749449864657 27.76674261455473 25.26435707191681 66-67 20.548772175809624 26.674261455473115 27.15623843742089 25.620727931296372 68-69 19.49718603337812 27.025763860489572 27.137738310841076 26.33931179529123 70-71 20.68022044361356 26.9196315553738 26.53697104243345 25.863176958579192 72-73 20.846721582831883 26.668419310237386 26.18254756479913 26.302311542131605 74-75 20.28587564020175 26.568129150357343 26.268232361589845 26.877762847851066 76 20.981090923253685 26.50970769800004 25.637280676130942 26.87192070261533 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.5 2 0.5 3 0.0 4 0.0 5 0.5 6 0.5 7 0.0 8 0.0 9 0.5 10 0.5 11 0.5 12 1.0 13 0.5 14 0.0 15 2.0 16 5.0 17 6.0 18 5.0 19 5.5 20 10.5 21 14.0 22 15.5 23 22.0 24 33.5 25 40.0 26 71.5 27 137.0 28 190.0 29 209.0 30 279.0 31 397.5 32 583.5 33 721.0 34 835.5 35 1121.5 36 1352.5 37 1412.0 38 1701.5 39 2221.5 40 2702.0 41 3133.5 42 3315.0 43 3434.0 44 3744.5 45 3979.5 46 4023.0 47 4004.5 48 3828.5 49 3434.0 50 3197.0 51 3009.0 52 2529.0 53 2067.5 54 1898.0 55 1754.5 56 1434.5 57 1048.5 58 839.0 59 704.0 60 484.0 61 334.0 62 269.0 63 212.0 64 136.0 65 92.0 66 66.5 67 66.0 68 53.5 69 37.0 70 33.0 71 33.0 72 28.0 73 17.0 74 8.5 75 6.0 76 6.0 77 5.5 78 5.0 79 5.0 80 5.5 81 4.5 82 3.5 83 4.0 84 3.0 85 2.0 86 1.0 87 0.0 88 0.5 89 1.5 90 1.0 91 0.5 92 1.0 93 0.5 94 1.0 95 1.5 96 1.0 97 1.5 98 2.0 99 1.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.6387412124398746 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 51351.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.68621837938891 #Duplication Level Percentage of deduplicated Percentage of total 1 78.96551724137932 49.500496582345036 2 8.940664802733767 11.209129325621701 3 4.283939111525319 8.056318280072443 4 2.503883193538366 6.278358746665108 5 1.6930723827275551 5.306615255788592 6 1.093507300403852 4.112870245954314 7 0.7424666045355701 3.2579696597924093 8 0.49394221808014915 2.4770695799497573 9 0.33550792171481825 1.8928550563767015 >10 0.9474992233612924 7.908317267433936 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.00584214523573056 0.0 5 0.0 0.0 0.0 0.00584214523573056 0.0 6 0.0 0.0 0.0 0.00584214523573056 0.0 7 0.0 0.0 0.0 0.00584214523573056 0.0 8 0.0 0.0 0.0 0.00584214523573056 0.0 9 0.0 0.0 0.0 0.01168429047146112 0.0 10 0.0 0.0 0.0 0.01752643570719168 0.0 11 0.0 0.0 0.0 0.02336858094292224 0.0 12 0.0 0.0 0.0 0.02336858094292224 0.0 13 0.0 0.0 0.0 0.027263344433409283 0.0 14 0.0 0.0 0.0 0.027263344433409283 0.0 15 0.0 0.0 0.0 0.03115810792389632 0.0 16 0.0 0.0 0.0 0.03505287141438336 0.0 17 0.0 0.0 0.0 0.0389476349048704 0.0 18 0.0 0.0 0.0 0.04478978014060096 0.0 19 0.0 0.0 0.0 0.04478978014060096 0.0 20 0.0 0.0 0.0 0.048684543631088005 0.0 21 0.0 0.0 0.0 0.05257930712157504 0.0 22 0.0 0.0 0.0 0.060368834102549125 0.0 23 0.0 0.0 0.0 0.07010574282876672 0.0 24 0.0 0.0 0.0 0.07594788806449729 0.0 25 0.0 0.0 0.0 0.07984265155498432 0.0 26 0.0 0.0 0.0 0.07984265155498432 0.0 27 0.0 0.0 0.0 0.11879028645985472 0.0 28 0.0 0.0 0.0 0.12463243169558529 0.0 29 0.0 0.0 0.0 0.13826410391228994 0.0 30 0.0 0.0 0.0 0.14410624914802048 0.0 31 0.0 0.0 0.0 0.17331697532667328 0.0 32 0.0 0.0 0.0 0.18889602928862145 0.0 33 0.0 0.0 0.0 0.2336858094292224 0.0 34 0.0 0.0 0.0 0.26679129909836224 0.0 35 0.0 0.0 0.0 0.3193706062199373 0.0 36 0.0 0.0 0.0 0.43037136569881795 0.0 37 0.0 0.0 0.0 0.6348464489493876 0.0 38 0.0 0.0 0.0 0.8860586940858016 0.0 39 0.0 0.0 0.0 1.2599559891725576 0.0 40 0.0 0.0 0.0 1.6942221183618624 0.0 41 0.0 0.0 0.0 2.2355942435395613 0.0 42 0.0 0.0 0.0 2.771124223481529 0.0 43 0.0 0.0 0.0 3.2073377344160776 0.0 44 0.0 0.0 0.0 3.7584467683199936 0.0 45 0.0 0.0 0.0 4.307608420478666 0.0 46 0.0 0.0 0.0 4.848980545656365 0.0 47 0.0 0.0 0.0 5.394247434324551 0.0 48 0.0 0.0 0.0 5.8771981071449435 0.0 49 0.0 0.0 0.0 6.426359759303616 0.0 50 0.0 0.0 0.0 7.010574282876672 0.0 51 0.0 0.0 0.0 7.6181573873926505 0.0 52 0.0 0.0 0.0 8.173161184787054 0.0 53 0.0 0.0 0.0 8.700901637748048 0.0 54 0.0 0.0 0.0 9.320169032735487 0.0 55 0.0 0.0 0.0 9.892699265837082 0.0 56 0.0 0.0 0.0 10.400965901345641 0.0 57 0.0 0.0 0.0 10.95986446223053 0.0 58 0.0 0.0 0.0 11.444762516796168 0.0 59 0.0 0.0 0.0 11.927713189616561 0.0 60 0.0 0.0 0.0 12.420400771163171 0.0 61 0.0 0.0 0.0 12.987088859029036 0.0 62 0.0 0.0 0.0 13.477829058830402 0.0 63 0.0 0.0 0.0 14.065938345893946 0.0 64 0.0 0.0 0.0 14.54888901871434 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GATACCT 15 0.0022135193 69.994156 28 TTATGAC 15 0.0022135193 69.994156 50 CCGTGCC 15 0.0022135193 69.994156 48 TACGGAC 15 0.0022135193 69.994156 38 TCCGTGC 15 0.0022135193 69.994156 47 TCTCCGT 15 0.0022135193 69.994156 45 TTCTATA 20 0.006916748 52.49562 2 CGTGGTA 20 0.006916748 52.49562 8 TGGAGAG 20 0.006916748 52.49562 44 GTTTCAT 20 0.006916748 52.49562 39 ATCAGCC 20 0.006916748 52.49562 19 GAATCGA 20 0.006916748 52.49562 15 CAGATCA 20 0.006916748 52.49562 14 CTAAAGC 20 0.006916748 52.49562 60 ATGACTC 20 0.006916748 52.49562 52 TTAAAAT 20 0.006916748 52.49562 21 GAACCCA 20 0.006916748 52.49562 20 GCTACAT 20 0.006916748 52.49562 29 CTTTACG 20 0.006916748 52.49562 35 AAGATAC 20 0.006916748 52.49562 26 >>END_MODULE