FastQCFastQC Report
Wed 25 May 2016
SRR1780568_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780568_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51351
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGT4540.8841113123405582No Hit
ATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGTCTT3310.6445833576756052TruSeq Adapter, Index 2 (95% over 21bp)
ATAAAGAATATTGAGGCGCCATTGGCGTGAAGGTAGCGGATGATTCAGCC1550.3018441705127456No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCC690.13436934042180287No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCATGC150.00221278770.024
ATATGGG252.3695163E-455.99999614
TCCGAAG200.006914464852.528
TTCCGAA200.006914464852.527
ATTCAAT200.006914464852.528
TATTCCG200.006914464852.525
CCTGGTA200.006914464852.535
CCGAAGC200.006914464852.529
ACTGGTG200.006914464852.58
GCATACT200.006914464852.529
CCAGTAA305.826801E-446.66666440
GAGGTGT404.533474E-543.7561
CAACCAG350.00124460140.037
CCTCGGA350.00124460140.045
ACCAGTA350.00124460140.039
ATAAAGA350.00124460140.01
AAAGAAT350.00124460140.03
TAAAGAA459.079676E-538.888892
AACCAGT400.002398046235.038
TATGCCG708.486768E-735.043
Produced by FastQC (version 0.11.5)