##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780568_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 51351 Sequences flagged as poor quality 0 Sequence length 76 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.797550193764486 34.0 34.0 34.0 31.0 34.0 2 33.27775505832408 34.0 34.0 34.0 32.0 34.0 3 33.41973866136979 34.0 34.0 34.0 34.0 34.0 4 33.45712839087847 34.0 34.0 34.0 34.0 34.0 5 33.45494732332379 34.0 34.0 34.0 34.0 34.0 6 37.17074643142295 38.0 37.0 38.0 36.0 38.0 7 36.75110513914043 38.0 37.0 38.0 34.0 38.0 8 36.752312515822474 38.0 37.0 38.0 34.0 38.0 9 36.51835407294892 38.0 37.0 38.0 34.0 38.0 10-11 36.19511791396468 38.0 36.5 38.0 33.0 38.0 12-13 36.082082140562015 38.0 37.0 38.0 33.0 38.0 14-15 36.340217327802776 38.0 37.0 38.0 34.0 38.0 16-17 36.472746392475315 38.0 37.0 38.0 34.0 38.0 18-19 36.48313567408619 38.0 37.0 38.0 34.0 38.0 20-21 36.200064263597596 38.0 37.0 38.0 33.5 38.0 22-23 36.228505773986875 38.0 37.0 38.0 33.0 38.0 24-25 35.535549453759415 38.0 36.0 38.0 29.0 38.0 26-27 35.765846818951914 38.0 36.0 38.0 31.5 38.0 28-29 35.58081634242761 38.0 36.0 38.0 31.0 38.0 30-31 35.2628673248817 38.0 36.0 38.0 29.0 38.0 32-33 35.813830305154724 38.0 36.5 38.0 31.5 38.0 34-35 35.8808202371911 38.0 36.5 38.0 32.5 38.0 36-37 36.0473895347705 38.0 37.0 38.0 34.0 38.0 38-39 35.714367782516405 38.0 36.5 38.0 32.5 38.0 40-41 35.14783548519016 37.0 35.5 38.0 29.0 38.0 42-43 35.57497419719188 37.5 36.0 38.0 31.5 38.0 44-45 35.807481840665226 38.0 36.5 38.0 31.5 38.0 46-47 36.1031333372281 38.0 37.0 38.0 34.0 38.0 48-49 35.861580105548086 38.0 36.5 38.0 32.0 38.0 50-51 36.02251173297502 38.0 37.0 38.0 33.5 38.0 52-53 35.95876419154447 38.0 37.0 38.0 32.5 38.0 54-55 36.13044536620514 38.0 37.0 38.0 34.0 38.0 56-57 36.20729878678117 38.0 37.0 38.0 34.0 38.0 58-59 36.25888492921267 38.0 37.0 38.0 34.0 38.0 60-61 35.93170532219431 38.0 37.0 38.0 32.5 38.0 62-63 36.22398784833791 38.0 37.0 38.0 34.0 38.0 64-65 36.23145605733092 38.0 37.0 38.0 34.0 38.0 66-67 35.552705886935016 38.0 36.0 38.0 31.0 38.0 68-69 34.94885201846118 37.5 35.5 38.0 27.0 38.0 70-71 34.91430546630056 37.0 35.0 38.0 27.0 38.0 72-73 34.65911082549512 37.0 35.0 38.0 26.0 38.0 74-75 35.36088878502853 38.0 36.0 38.0 30.5 38.0 76 34.80298338883372 37.0 35.0 38.0 27.0 38.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.45977766459461833 1101 2 0.4756730974981096 1101 3 0.013282062070700817 1101 4 0.5870167055775397 1101 5 0.349729842726191 1101 6 -0.6966293745776753 1101 7 0.16709295122974765 1101 8 0.9166522813650531 1101 9 0.20781420587553612 1101 10-11 0.20792410415069895 1101 12-13 0.579104873118105 1101 14-15 0.7337910082854577 1101 16-17 -0.05841937167104305 1101 18-19 -0.2051178985057689 1101 20-21 0.14422846479209994 1101 22-23 0.8472006821505573 1101 24-25 -0.9972695298017982 1101 26-27 -0.23977720809615022 1101 28-29 0.8479761724038113 1101 30-31 1.1416912869017324 1101 32-33 -0.06772001069400346 1101 34-35 0.7181973202317593 1101 36-37 0.7958335301141659 1101 38-39 0.5047845197486751 1101 40-41 1.4867025429764382 1101 42-43 0.6792871798304603 1101 44-45 0.4682418635929295 1101 46-47 0.4696041081900759 1101 48-49 -1.482453921372013 1101 50-51 -1.5544043828010814 1101 52-53 -1.8969820000272861 1101 54-55 -1.2362564310065949 1101 56-57 -1.3485281869964254 1101 58-59 -1.4553078484430415 1101 60-61 -1.210902137657115 1101 62-63 -0.2361876400109324 1101 64-65 -1.213782028323294 1101 66-67 -1.196281096812065 1101 68-69 -1.4832154418404926 1101 70-71 -1.3894628814399752 1101 72-73 -1.360822876759869 1101 74-75 -0.8074783086313388 1101 76 -1.0350480498234518 1102 1 -0.5150624206932264 1102 2 0.4456091316132458 1102 3 0.38726926889373203 1102 4 0.4886158526990769 1102 5 0.13981513057267136 1102 6 0.3624324591110195 1102 7 0.2296729085858189 1102 8 0.4686778677189949 1102 9 0.7942747602465374 1102 10-11 0.7995552342146652 1102 12-13 0.5085824850584046 1102 14-15 -0.04589982327104991 1102 16-17 -0.07115924373927385 1102 18-19 -0.4015464699343383 1102 20-21 0.6465205756663011 1102 22-23 0.7644714710631391 1102 24-25 -5.879039252616302 1102 26-27 -1.4912164405055321 1102 28-29 0.40378640694538603 1102 30-31 1.588546297562715 1102 32-33 0.2663418229946899 1102 34-35 0.8140395377157716 1102 36-37 -1.9979831010159046 1102 38-39 0.009102216976820898 1102 40-41 1.0658603254924301 1102 42-43 0.7776347278048732 1102 44-45 1.0515041237208607 1102 46-47 0.9676851316442381 1102 48-49 -3.3500445397088967 1102 50-51 -0.3209822079610021 1102 52-53 0.150299449866111 1102 54-55 0.2326668099315654 1102 56-57 0.7218342863511253 1102 58-59 -0.30626733671596185 1102 60-61 -6.145177190962016 1102 62-63 -2.5563368937422766 1102 64-65 0.18813694822254945 1102 66-67 1.5290920375162855 1102 68-69 -0.21258644397268966 1102 70-71 -1.223844544552982 1102 72-73 -6.300001981237482 1102 74-75 -2.4700582659874186 1102 76 -0.3049840839385851 1103 1 0.6031193974885909 1103 2 0.5858688718729894 1103 3 0.20934719097164844 1103 4 0.4490054630886888 1103 5 0.31319175394929744 1103 6 0.4325623292408949 1103 7 0.9692832981962098 1103 8 0.5699765690176974 1103 9 0.22089813686991278 1103 10-11 0.3719578316172587 1103 12-13 -0.3326512811753659 1103 14-15 -0.7923283946996165 1103 16-17 0.408386210806178 1103 18-19 0.6520249586370923 1103 20-21 -0.4895183853726621 1103 22-23 0.47745848405015323 1103 24-25 -3.964753538330587 1103 26-27 -0.41296968725878713 1103 28-29 0.08852666668564524 1103 30-31 0.588870972887392 1103 32-33 1.08419896585184 1103 34-35 -0.21647994280370852 1103 36-37 -1.1194116724444712 1103 38-39 -0.7451185622439525 1103 40-41 0.1648862995184004 1103 42-43 -0.12333929816915656 1103 44-45 0.9878677600844981 1103 46-47 1.2397630537221573 1103 48-49 -2.152641942306296 1103 50-51 -0.8362419482207386 1103 52-53 0.06880594337260248 1103 54-55 -0.5046708524060932 1103 56-57 0.6276784421952755 1103 58-59 -0.5231504535990794 1103 60-61 -3.8948525156373393 1103 62-63 -1.4336096210150089 1103 64-65 0.2822927923783922 1103 66-67 -0.6572715988473448 1103 68-69 -0.5635604699467152 1103 70-71 -0.19819519390363638 1103 72-73 -5.09643055266605 1103 74-75 -2.2655128114419654 1103 76 -4.08680226575677 1104 1 0.12079377609012454 1104 2 0.16490903700587012 1104 3 0.14251997844907294 1104 4 0.11439636357892624 1104 5 -0.36174589118702727 1104 6 0.16602753952729188 1104 7 0.5919718679046468 1104 8 0.7674479717871137 1104 9 1.1347194149295987 1104 10-11 -0.5348108963435223 1104 12-13 0.6554367896941642 1104 14-15 1.0089961086116404 1104 16-17 0.31926175909894994 1104 18-19 0.9720344193750279 1104 20-21 0.25510619534463785 1104 22-23 0.36939105479066825 1104 24-25 0.570487710486816 1104 26-27 1.1173691791728046 1104 28-29 1.5359765677779293 1104 30-31 1.2846437431634712 1104 32-33 0.8495726650003661 1104 34-35 0.1774737855871038 1104 36-37 1.136028251869618 1104 38-39 1.706524165888844 1104 40-41 1.767492302786657 1104 42-43 0.7190964118162242 1104 44-45 0.9496592798230381 1104 46-47 1.3254902404427256 1104 48-49 0.3157075889571388 1104 50-51 0.6078966945933999 1104 52-53 0.7713732436220226 1104 54-55 0.537515438124565 1104 56-57 -0.014684162087860386 1104 58-59 -0.5223978948994983 1104 60-61 0.7382428658024054 1104 62-63 0.7737955565888512 1104 64-65 0.8210603162452514 1104 66-67 -0.06395432009960444 1104 68-69 1.8689887688939208 1104 70-71 1.6576360609342444 1104 72-73 1.8607596081664894 1104 74-75 0.5557222448924293 1104 76 0.34525243450985243 1105 1 0.823571900959962 1105 2 0.5980799312538565 1105 3 0.37172569297279523 1105 4 0.14634271702971802 1105 5 -0.14890903834017877 1105 6 0.08085114734102916 1105 7 0.4700592518023541 1105 8 -0.7467758609422788 1105 9 0.43277431101023467 1105 10-11 0.6824617930646255 1105 12-13 -0.6230212885265018 1105 14-15 0.13219990718717867 1105 16-17 0.2905433618312614 1105 18-19 0.5071462524376287 1105 20-21 0.584503504686964 1105 22-23 0.28163229765792863 1105 24-25 0.7331350510674355 1105 26-27 0.07569730612519976 1105 28-29 1.2146803871789942 1105 30-31 1.2003836740227314 1105 32-33 0.9251693162561452 1105 34-35 -0.16498112715395052 1105 36-37 0.6964014452554323 1105 38-39 0.333518209789041 1105 40-41 0.1701954557709584 1105 42-43 0.12815134954791318 1105 44-45 1.017946172238382 1105 46-47 1.0128783032524993 1105 48-49 0.16999139919496287 1105 50-51 0.8748174326499694 1105 52-53 0.6339247867933366 1105 54-55 0.9631474927707444 1105 56-57 0.972508140798567 1105 58-59 0.7094110100944633 1105 60-61 0.6450069959202835 1105 62-63 -0.24721739688693134 1105 64-65 0.7743903174947775 1105 66-67 0.6709159368873614 1105 68-69 0.7811467096661247 1105 70-71 1.105468123910633 1105 72-73 1.3570734904606354 1105 74-75 0.037354132934417805 1105 76 0.4494633554145082 1106 1 -0.6730158148168357 1106 2 0.28481885805701523 1106 3 0.5162459659555338 1106 4 0.2626280107537866 1106 5 0.18576244566892086 1106 6 0.2878630568820455 1106 7 -0.5429208097525802 1106 8 0.5667528423897181 1106 9 -0.2357657666025048 1106 10-11 0.8353455077708958 1106 12-13 1.4335824850584018 1106 14-15 -0.0724702386712508 1106 16-17 0.31275666304897953 1106 18-19 0.6689955766715414 1106 20-21 0.6835266546936509 1106 22-23 0.8459304376284891 1106 24-25 0.38555041303719406 1106 26-27 1.435860560507642 1106 28-29 0.8292929925583579 1106 30-31 0.9335564292749723 1106 32-33 0.3653539810494024 1106 34-35 1.4261469338657236 1106 36-37 1.190112137079339 1106 38-39 0.40872227776710446 1106 40-41 1.1883273974275852 1106 42-43 1.1549143630632273 1106 44-45 1.3117371531028255 1106 46-47 1.2404308256665288 1106 48-49 1.3943323599871533 1106 50-51 0.365517285453393 1106 52-53 0.5041241712440225 1106 54-55 0.24074685045841449 1106 56-57 0.6620571840208314 1106 58-59 0.8574104748341895 1106 60-61 1.1294175405476068 1106 62-63 0.988698567250637 1106 64-65 -0.7363817954451335 1106 66-67 0.7095884914169943 1106 68-69 1.7747235864224464 1106 70-71 -0.01616977251650553 1106 72-73 -0.6083353145708159 1106 74-75 -0.1116489448121385 1106 76 0.365026047773668 1107 1 0.9268848040565487 1107 2 -0.44237994936946734 1107 3 0.24814279027680186 1107 4 0.2166868263114523 1107 5 0.08335471201034039 1107 6 0.24942063017562788 1107 7 0.9818567120435091 1107 8 0.29087986953883416 1107 9 -0.22487901591360782 1107 10-11 0.8045825317578874 1107 12-13 -0.047309234686821355 1107 14-15 0.1280537709054741 1107 16-17 0.7743594095819262 1107 18-19 0.9047547129591962 1107 20-21 0.6892184828546561 1107 22-23 0.09731951473921185 1107 24-25 0.692434814717636 1107 26-27 -0.06635292822164018 1107 28-29 0.9670939592656751 1107 30-31 1.1680958880995718 1107 32-33 0.9468013316389161 1107 34-35 0.8664053247949326 1107 36-37 1.1890505659540693 1107 38-39 0.3841932087875577 1107 40-41 0.4865837103877837 1107 42-43 0.9422161654754788 1107 44-45 0.7762084003359675 1107 46-47 1.0267388168125748 1107 48-49 0.7440409925931917 1107 50-51 1.086911331620442 1107 52-53 0.43448962275055436 1107 54-55 0.871952524217285 1107 56-57 1.009823367333837 1107 58-59 0.4824951746580197 1107 60-61 1.2032532003027683 1107 62-63 0.9401599215443497 1107 64-65 1.248191543308991 1107 66-67 0.45042961713412666 1107 68-69 1.2592743385568852 1107 70-71 0.45001805781280524 1107 72-73 1.3492018404185728 1107 74-75 -0.8260637254960628 1107 76 1.268355315515464 1108 1 -0.08254362370074375 1108 2 0.08997003386888736 1108 3 -0.19017434012882717 1108 4 0.4138038226238905 1108 5 0.3881234012493593 1108 6 0.1902520079832044 1108 7 0.3663270439241657 1108 8 -0.2948559668674733 1108 9 0.005928895584879967 1108 10-11 0.7078259109063936 1108 12-13 1.203319327163662 1108 14-15 0.45109265793195874 1108 16-17 -0.21204270238588663 1108 18-19 0.4297505225468612 1108 20-21 0.9696408764181825 1108 22-23 0.4870278620405841 1108 24-25 1.3590246571581304 1108 26-27 -0.5200698239641781 1108 28-29 -1.00767975846815 1108 30-31 -0.39331460469292523 1108 32-33 0.6086350560774036 1108 34-35 0.8021034474902038 1108 36-37 0.9444981019916128 1108 38-39 1.1088202620896013 1108 40-41 1.0146385209811442 1108 42-43 -0.43712091129287245 1108 44-45 0.9680454771043188 1108 46-47 1.151801672997621 1108 48-49 0.9952562121708013 1108 50-51 0.9557095213623157 1108 52-53 -0.27620430953238895 1108 54-55 0.0035314715857097667 1108 56-57 -0.13436872116767518 1108 58-59 0.30519882869757 1108 60-61 0.8555746887372351 1108 62-63 0.96471573783667 1108 64-65 0.46586251213231833 1108 66-67 0.8244867743583981 1108 68-69 1.6264173154258117 1108 70-71 -0.15222800319959617 1108 72-73 0.6164453871835747 1108 74-75 -0.15802819079944896 1108 76 0.6730234348584005 1109 1 -0.5427409921218 1109 2 0.3741805601846764 1109 3 0.32923355460801673 1109 4 0.07433013841336589 1109 5 -0.1066134408559023 1109 6 -0.589353255174693 1109 7 -0.25604137712846153 1109 8 0.3784992962904212 1109 9 0.8210604745322527 1109 10-11 0.533483805643236 1109 12-13 0.7867074850584075 1109 14-15 0.9264216053003835 1109 16-17 0.7703586134035803 1109 18-19 0.8636321014942254 1109 20-21 1.1768777185234427 1109 22-23 0.4533107567774266 1109 24-25 -0.293771395473442 1109 26-27 0.4297657023516095 1109 28-29 -0.6060350216260417 1109 30-31 0.4255998689912843 1109 32-33 0.6926811087089817 1109 34-35 0.033682394858630005 1109 36-37 0.9203204704126691 1109 38-39 0.7524057884053974 1109 40-41 0.11496746834956895 1109 42-43 0.949063299233444 1109 44-45 0.9256112665780023 1109 46-47 0.8935779887870936 1109 48-49 0.8695983174339617 1109 50-51 0.2691963634675787 1109 52-53 -0.03541483584817939 1109 54-55 -0.09679747578271503 1109 56-57 0.20576285777968906 1109 58-59 0.1973040918554645 1109 60-61 0.6633049518951282 1109 62-63 0.10303810625772769 1109 64-65 0.9528690910796911 1109 66-67 0.16971703751628553 1109 68-69 0.2012528417415993 1109 70-71 0.5770483125898735 1109 72-73 0.02187301876251979 1109 74-75 0.2500310197268689 1109 76 1.02894448748998 1110 1 -0.7999138109137434 1110 2 -0.4405845404864692 1110 3 0.1971446284335201 1110 4 0.04043752096369957 1110 5 0.511004008808527 1110 6 -0.16527607396663768 1110 7 0.6026834550863001 1110 8 0.7938935915924361 1110 9 0.7867483939953388 1110 10-11 -0.7647544493903169 1110 12-13 0.2456630219711542 1110 14-15 1.2043159006023956 1110 16-17 0.5207151570277446 1110 18-19 0.0046136451183897975 1110 20-21 1.1596797319462553 1110 22-23 -0.34377397476619365 1110 24-25 -0.8638427041982766 1110 26-27 1.0991239238281238 1110 28-29 0.9203492065618804 1110 30-31 0.3322903052329025 1110 32-33 1.1532306053532722 1110 34-35 1.1882964888183594 1110 36-37 1.3825058730972302 1110 38-39 1.2615710568617757 1110 40-41 0.21626780392004008 1110 42-43 0.8733921582938464 1110 44-45 1.0899988504706215 1110 46-47 0.847646780733406 1110 48-49 1.3554204650849684 1110 50-51 -0.5111308177404794 1110 52-53 0.8483099963665879 1110 54-55 -0.14818170397063568 1110 56-57 0.7328601732159399 1110 58-59 -0.3820583242519149 1110 60-61 -0.08168246421225689 1110 62-63 -0.3741221286416021 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2116 52-53 -0.08226357534397977 2116 54-55 -0.5356105009927958 2116 56-57 -0.6605186548253457 2116 58-59 -2.3747547316739457 2116 60-61 -1.528160384239328 2116 62-63 0.24744461886276525 2116 64-65 -2.349678177827869 2116 66-67 -0.827630651559339 2116 68-69 -0.49346879691386647 2116 70-71 0.810399152926017 2116 72-73 0.14936776666168328 2116 74-75 0.1664963558613195 2116 76 -0.8839756805772438 2117 1 0.5551756745448699 2117 2 0.6991805601846721 2117 3 0.3063168879413496 2117 4 -0.7673365282533027 2117 5 0.15171989247743056 2117 6 0.28981341149197704 2117 7 0.5439586228715356 2117 8 -0.42775070370957735 2117 9 -1.3581061921344144 2117 10-11 -0.4790161943567668 2117 12-13 -1.4122508482749296 2117 14-15 -0.1839950613662822 2117 16-17 -1.1681830532630855 2117 18-19 -1.5634512318391032 2117 20-21 -0.9356222814765545 2117 22-23 0.12101909011076373 2117 24-25 -1.3468963954734434 2117 26-27 -2.619192630981722 2117 28-29 -1.3414516882927074 2117 30-31 -0.57335846434205 2117 32-33 -2.610443891291027 2117 34-35 0.24618239485862858 2117 36-37 -1.3848878629206638 2117 38-39 0.009697455072064542 2117 40-41 0.008717468349566104 2117 42-43 -0.5634367007665588 2117 44-45 -0.43063873342199344 2117 46-47 -1.037672011212912 2117 48-49 -0.780401682566044 2117 50-51 -0.6453869698657613 2117 52-53 0.7770851641518206 2117 54-55 -0.6113808091160493 2117 56-57 0.21409619111302902 2117 58-59 -0.6651959081445327 2117 60-61 -0.11898671477153755 2117 62-63 -0.8063368937422766 2117 64-65 -0.46067257558698316 2117 66-67 -1.0459079624837173 2117 68-69 -1.0102054915917336 2117 70-71 -0.662535020743455 2117 72-73 0.9041646854291869 2117 74-75 1.0323226863935346 2117 76 0.90811115415665 2118 1 -1.0722052778360833 2118 2 0.12712173665526194 2118 3 -0.9816382941314785 2118 4 0.019288121606642505 2118 5 0.34107563477434866 2118 6 -0.0930297257629249 2118 7 0.8317737489219539 2118 8 0.6838039181391622 2118 9 -1.878414315383715 2118 10-11 -0.7254447657853333 2118 12-13 -0.8983502880508425 2118 14-15 0.19086488261130796 2118 16-17 -2.8885962185291874 2118 18-19 -0.8878910077494666 2118 20-21 -1.1566306848379 2118 22-23 0.5737151685421367 2118 24-25 0.4575153692324392 2118 26-27 -2.5926870287408263 2118 28-29 -1.1333984670041914 2118 30-31 -1.122167988151574 2118 32-33 -0.24353212658513712 2118 34-35 -0.6435234874943134 2118 36-37 -3.816890664041111 2118 38-39 -0.11505744688872 2118 40-41 0.6698519221310804 2118 42-43 0.6967523748636992 2118 44-45 -1.8312689855228328 2118 46-47 -0.05836528852383083 2118 48-49 0.20373697289614512 2118 50-51 -0.9951418718265401 2118 52-53 -1.615877020722131 2118 54-55 -0.6532575598163319 2118 56-57 0.6293973115612062 2118 58-59 -0.6562673367159633 2118 60-61 -1.0155553422225267 2118 62-63 -0.9248242887002576 2118 64-65 0.11250669612170583 2118 66-67 0.046318928272590654 2118 68-69 -1.5817040910315114 2118 70-71 -0.8996848806874382 2118 72-73 1.292224909518822 2118 74-75 -0.14022633321430789 2118 76 0.13283104211183172 2119 1 0.6735090078782022 2119 2 -0.7458194398153282 2119 3 -0.32701644539198327 2119 4 -1.155669861586638 2119 5 -0.45161344085590116 2119 6 -0.5018532551746944 2119 7 -1.5460413771284678 2119 8 -0.21275070370957394 2119 9 -1.171439525467747 2119 10-11 -0.7640161943567634 2119 12-13 -0.1814175149416002 2119 14-15 -0.6773283946996145 2119 16-17 -0.6940163865964095 2119 18-19 -0.5801178985057689 2119 20-21 -1.4156222814765584 2119 22-23 -0.3848142432225714 2119 24-25 0.503103604526558 2119 26-27 0.4616407023516089 2119 28-29 -0.9747850216260403 2119 30-31 0.5449748689912894 2119 32-33 -0.3829438912910206 2119 34-35 0.11618239485863313 2119 36-37 0.13844547041266253 2119 38-39 -2.0344692115946046 2119 40-41 -1.2312825316504288 2119 42-43 -0.005936700766561387 2119 44-45 -0.09063873342199003 2119 46-47 -0.4401720112129084 2119 48-49 -1.4729016825660395 2119 50-51 0.6154463634675764 2119 52-53 -0.1254148358481757 2119 54-55 -0.07804747578271787 2119 56-57 -1.261737142220305 2119 58-59 -0.19269590814453608 2119 60-61 -0.1173200481048724 2119 62-63 -0.5913368937422803 2119 64-65 -0.8940059089203132 2119 66-67 0.21909203751629036 2119 68-69 0.7281278417416033 2119 70-71 -0.08670168741011963 2119 72-73 1.1349980187625164 2119 74-75 -0.7943439802731334 2119 76 0.47644448748998514 >>END_MODULE >>Per sequence quality scores pass #Quality Count 19 1.0 20 0.0 21 3.0 22 4.0 23 11.0 24 24.0 25 37.0 26 96.0 27 191.0 28 323.0 29 565.0 30 901.0 31 1348.0 32 2105.0 33 3270.0 34 4867.0 35 7556.0 36 12722.0 37 17327.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 36.3342486027536 24.122217678331484 11.436972989815192 28.106560729099726 2 17.21290724620747 19.32386905805145 39.50458608401004 23.958637611731028 3 16.803957079706336 25.67038616580008 31.054896691398415 26.470760063095167 4 12.654086580592395 19.662713481723824 39.53769157367919 28.145508364004595 5 11.990029405464353 37.66431033475492 37.37804521820413 12.9676150415766 6 26.749235652664993 40.195906603571494 18.021070670483535 15.033787073279974 7 25.393857957975502 30.725789176452263 23.925532122061888 19.95482074351035 8 22.221573094973806 36.36151194718701 21.070670483534887 20.3462444743043 9 22.63247064322019 14.827364608284164 23.610056279332436 38.93010846916321 10-11 24.233218437810365 26.09686276800841 28.01503378707328 21.654885007107943 12-13 23.748320383244728 23.059920936301143 27.404529609939438 25.787229070514694 14-15 21.003485813323984 25.155303694183175 24.545773207921947 29.295437284570898 16-17 22.495180230180523 28.226324706432205 25.71907070943117 23.559424353956107 18-19 22.562364900391422 27.081264240229014 26.57786605908356 23.778504800296 20-21 21.465988977819322 27.430819263500222 26.407470156374753 24.6957216023057 22-23 21.91778154271582 28.111429183462832 27.091974839827852 22.878814433993497 24-25 22.06188779186384 28.662538217366752 26.629471675332518 22.646102315436895 26-27 21.88467605304668 28.5038266051294 27.137738310841076 22.473759030982844 28-29 22.55554906428307 26.78818328756986 28.056902494596013 22.59936515355105 30-31 21.849623181632296 28.006270569219684 27.21758096239606 22.92652528675196 32-33 21.43093610640494 28.36848357383498 26.67718252809098 23.5233977916691 34-35 21.909992015734844 27.209791435415088 28.115323946953318 22.76489260189675 36-37 21.82528090981675 27.50092500632899 28.090007984265153 22.583786099589105 38-39 21.646121789254348 27.22342310763179 27.73266343401297 23.397791669100894 40-41 21.040486066483613 26.99850051605616 28.734591341940764 23.226422075519466 42-43 22.018071702595858 26.424996592081946 29.086093746957214 22.470837958364978 44-45 20.428034507604526 27.110474966407665 28.8572763918911 23.604214134096708 46-47 21.62372689918405 26.003388444236723 28.906934626394815 23.46595003018442 48-49 21.193355533485228 26.42110182859146 29.058830402523807 23.326712235399505 50-51 19.97624194270803 26.030651788670134 29.51354404003817 24.47956222858367 52-53 19.68997682615723 26.619734766606296 30.65081497926038 23.039473427976088 54-55 18.835076239995328 26.893341901813013 29.92931004264766 24.342271815544 56-57 20.327744347724487 26.00144106249148 29.058830402523807 24.611984187260227 58-59 19.42805398142198 26.96928978987751 29.38209577223423 24.22056045646628 60-61 20.286849331074368 27.520398823781427 27.754084633210645 24.438667211933556 62-63 18.768865260657048 28.936145352573465 27.49313547934802 24.801853907421474 64-65 19.321921676306207 29.48433331385952 26.478549590076145 24.715195419758135 66-67 18.9772351073981 29.672255652275513 26.131915639422797 25.21859360090358 68-69 18.972366653034996 29.190278670327746 26.71515647212323 25.122198204514028 70-71 19.917820490350724 28.7141438336157 26.11341551284298 25.254620163190587 72-73 20.351112928667405 28.177640162801115 25.11148760491519 26.359759303616286 74-75 20.65685186267064 28.320772721076516 24.732721855465325 26.289653560787524 76 20.611088391657418 28.23703530603104 25.07059258826508 26.081283714046467 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.5 12 1.0 13 0.5 14 1.0 15 1.5 16 1.5 17 2.0 18 1.5 19 5.0 20 7.5 21 6.0 22 10.5 23 34.5 24 52.5 25 51.0 26 83.0 27 136.5 28 209.0 29 260.0 30 306.5 31 408.0 32 572.5 33 682.0 34 888.0 35 1248.5 36 1496.5 37 1590.0 38 1838.5 39 2365.0 40 2957.0 41 3442.0 42 3613.0 43 3794.5 44 4176.0 45 4245.0 46 4114.0 47 4113.0 48 3914.0 49 3408.0 50 3100.0 51 2845.0 52 2329.5 53 1847.5 54 1626.0 55 1554.0 56 1153.5 57 751.5 58 678.0 59 510.5 60 288.0 61 180.5 62 128.0 63 94.5 64 45.0 65 21.0 66 9.0 67 5.0 68 2.5 69 0.0 70 0.0 71 0.0 72 0.0 73 0.0 74 0.5 75 1.0 76 0.5 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 51351.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 42.70413429144515 #Duplication Level Percentage of deduplicated Percentage of total 1 61.849605545168494 26.412338610737862 2 13.958684846550232 11.921871044380831 3 7.911897487345525 10.136121983992522 4 4.82922157873136 8.249109072851551 5 2.9185097359660723 6.231621584779265 6 2.0520771581011448 5.257930712157504 7 1.5458981257695288 4.621136881462872 8 1.117242008299512 3.8168682206773 9 0.8299512061653518 3.1898112987088862 >10 2.968671622052989 18.198282409300695 >50 0.004560171462446989 0.13436934042180287 >100 0.013680514387340964 1.830538840528909 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGT 454 0.8841113123405582 No Hit ATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGTCTT 331 0.6445833576756052 TruSeq Adapter, Index 2 (95% over 21bp) ATAAAGAATATTGAGGCGCCATTGGCGTGAAGGTAGCGGATGATTCAGCC 155 0.3018441705127456 No Hit CTCTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCC 69 0.13436934042180287 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.00778952698097408 0.0 5 0.0 0.0 0.0 0.00778952698097408 0.0 6 0.0 0.0 0.0 0.00778952698097408 0.0 7 0.0 0.0 0.0 0.00778952698097408 0.0 8 0.0 0.0 0.0 0.00778952698097408 0.0 9 0.0 0.0 0.0 0.01752643570719168 0.0 10 0.0 0.0 0.0 0.02531596268816576 0.0 11 0.0 0.0 0.0 0.03115810792389632 0.0 12 0.0 0.0 0.0 0.03115810792389632 0.0 13 0.0 0.0 0.0 0.03505287141438336 0.0 14 0.0 0.0 0.0 0.03505287141438336 0.0 15 0.0 0.0 0.0 0.0389476349048704 0.0 16 0.0 0.0 0.0 0.042842398395357445 0.0 17 0.0 0.0 0.0 0.04478978014060096 0.0 18 0.0 0.0 0.0 0.05257930712157504 0.0 19 0.0 0.0 0.0 0.05257930712157504 0.0 20 0.0 0.0 0.0 0.05647407061206208 0.0 21 0.0 0.0 0.0 0.06231621584779264 0.0 22 0.0 0.0 0.0 0.07205312457401024 0.0 23 0.0 0.0 0.0 0.08373741504547136 0.0 24 0.0 0.0 0.0 0.09347432377168896 0.0 25 0.0 0.0 0.0 0.09736908726217601 0.0 26 0.0 0.0 0.0 0.09736908726217601 0.0 27 0.0 0.0 0.0 0.12463243169558529 0.0 28 0.0 0.0 0.0 0.13242195867655937 0.0 29 0.0 0.0 0.0 0.14800101263850754 0.0 30 0.0 0.0 0.0 0.15773792136472511 0.0 31 0.0 0.0 0.0 0.19084341103386496 0.0 32 0.0 0.0 0.0 0.20642246499581313 0.0 33 0.0 0.0 0.0 0.2609491538626317 0.0 34 0.0 0.0 0.0 0.2960020252770151 0.0 35 0.0 0.0 0.0 0.35442347763432064 0.0 36 0.0 0.0 0.0 0.482950672820393 0.0 37 0.0 0.0 0.0 0.7166364822496154 0.0 38 0.0 0.0 0.0 0.969796109131273 0.0 39 0.0 0.0 0.0 1.3787462756324123 0.0 40 0.0 0.0 0.0 1.8539074214718312 0.0 41 0.0 0.0 0.0 2.4614905259878093 0.0 42 0.0 0.0 0.0 3.0495998130513526 0.0 43 0.0 0.0 0.0 3.550076921578937 0.0 44 0.0 0.0 0.0 4.161554789585402 0.0 45 0.0 0.0 0.0 4.761348367120407 0.0 46 0.0 0.0 0.0 5.343615508948219 0.0 47 0.0 0.0 0.0 5.968725049171389 0.0 48 0.0 0.0 0.0 6.525676228311036 0.0 49 0.0 0.0 0.0 7.141048859807988 0.0 50 0.0 0.0 0.0 7.770053163521645 0.0 51 0.0 0.0 0.0 8.461373683083094 0.0 52 0.0 0.0 0.0 9.068956787599072 0.0 53 0.0 0.0 0.0 9.655118692917373 0.0 54 0.0 0.0 0.0 10.35617612120504 0.0 55 0.0 0.0 0.0 10.98712780666394 0.0 56 0.0 0.0 0.0 11.5401842223131 0.0 57 0.0 0.0 0.0 12.173083289517244 0.0 58 0.0 0.0 0.0 12.726139705166403 0.0 59 0.0 0.0 0.0 13.28309088430605 0.0 60 0.0 0.0 0.0 13.812778719012288 0.0 61 0.0 0.0 0.0 14.463204221923624 0.0 62 0.0 0.0 0.0 15.018208019318028 0.0 63 0.0 0.0 0.0 15.6569492317579 0.0 64 0.0 0.0 0.0 16.17105801250219 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATCATGC 15 0.002212787 70.0 24 ATATGGG 25 2.3695163E-4 55.999996 14 TCCGAAG 20 0.0069144648 52.5 28 TTCCGAA 20 0.0069144648 52.5 27 ATTCAAT 20 0.0069144648 52.5 28 TATTCCG 20 0.0069144648 52.5 25 CCTGGTA 20 0.0069144648 52.5 35 CCGAAGC 20 0.0069144648 52.5 29 ACTGGTG 20 0.0069144648 52.5 8 GCATACT 20 0.0069144648 52.5 29 CCAGTAA 30 5.826801E-4 46.666664 40 GAGGTGT 40 4.533474E-5 43.75 61 CAACCAG 35 0.001244601 40.0 37 CCTCGGA 35 0.001244601 40.0 45 ACCAGTA 35 0.001244601 40.0 39 ATAAAGA 35 0.001244601 40.0 1 AAAGAAT 35 0.001244601 40.0 3 TAAAGAA 45 9.079676E-5 38.88889 2 AACCAGT 40 0.0023980462 35.0 38 TATGCCG 70 8.486768E-7 35.0 43 >>END_MODULE