FastQCFastQC Report
Wed 25 May 2016
SRR1780567_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780567_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences119
Sequences flagged as poor quality0
Sequence length50
%GC61

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCCGT32.5210084033613445No Hit
AGCCCACGAGACGATCCAAAATCTCGTATGCCGTCTTCTGCTTGAAAAAA21.680672268907563Illumina PCR Primer Index 6 (96% over 29bp)
CCCCATCTCAGCCTCCCTAGTAGCTGTGACTACAGGTGTGTGTCACCATT10.8403361344537815No Hit
GGGCAGGCCGAGCTCGACGCCCGGGCCGAGCAGCTGGCCGCCTGTCTCTT10.8403361344537815No Hit
TTATTGGGGAGGGGGTGATCTAAAACACTCTTTACGCCGGCTTCTATTGA10.8403361344537815No Hit
GCGTTCGCGGAACGCCGCGCGCCGTGCCTTCAGCCCGCTTTCGCTGACTC10.8403361344537815No Hit
GTCCACGGCGGGGCGCATGTGCTCCGTGTGGAACCTGTCTCTTATACACA10.8403361344537815No Hit
CACATAGAGGGTGTGCAGGGCCGGGGCGTCGAAGCCGGTGAGGAGCATGT10.8403361344537815No Hit
CACTATGTCCTATCAATAGGAGCTGTATTTGCCATCATAGGAGGCTTCAT10.8403361344537815No Hit
ATCTCCGAGCCCAGAGACTAACAACAATCTCGTATGCCGTCTTCTGCTTG10.8403361344537815RNA PCR Primer, Index 36 (96% over 30bp)
GTGTGGTCGGGTGTGTTATTATTCTGAATTTTGGGGGAGGTTATATGCTG10.8403361344537815No Hit
ACTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTCAA10.8403361344537815No Hit
AACATGACTAACACCCTTAATTCCATCCACCCTCCTCTCCCTCTGTCTCT10.8403361344537815No Hit
GCCCAGCGCGTGGTCGATCGGCACCGAGAACCAGTGGTCCACCGTGACGG10.8403361344537815No Hit
TCCCGACCCCGGCCCCCGCCCGCGTGGCGCGGCGGCCCCGACCCTGTCTC10.8403361344537815No Hit
CTAATCCAAACTCTAACACTATGCTTAGGCGCTATCACCACTCTGTTCGC10.8403361344537815No Hit
CGACTGAGCCCCGGCTGGGAGGCGCCTTCGACGCGCTGTCTCTTATACAC10.8403361344537815No Hit
ACCCCACCACAGTGCCCCTCTCCTGAACCAATCACTGTCTCTTATACACA10.8403361344537815No Hit
GGTTTACCGAGCCCACGAGACTAACAACAATCTCGTATGCCGTCTTCTGC10.8403361344537815RNA PCR Primer, Index 36 (100% over 26bp)
AGCACCTAGTTGGCCAGGATTAGAGTTTCAGACTGGGTTCTGGTGGCCCA10.8403361344537815No Hit
CCCCGCGGCGGCGGGCACCGAGACGGCGCTCGTGACACTGTCTCTTATAC10.8403361344537815No Hit
AGTTATGGAGGCCATGGGGTTGGCTTGAAACCAGCTTTGGGGGGTTCGAT10.8403361344537815No Hit
CTTATACACATCTCCGAGCCCAAGAGACCCAACACGATCTCGTATGCCGT10.8403361344537815TruSeq Adapter, Index 1 (95% over 21bp)
CCCCTCGAGCCGGCCCGCCACCGCGTGGGCGAGCGCCTGTCTCTTATACA10.8403361344537815No Hit
ACCTCGGGCGGCACGACGGCGGCGTCGAAGCGCACGCGGACCGCTTCGGC10.8403361344537815No Hit
CTACACCCCGGTGCCCCGTTCGGCGGCGGAGGCCGAGTTCCTGGCCATCG10.8403361344537815No Hit
GGGTACGGGCGCGGCGCGCGCATGAAGTTCGAGCAGGACGCGGTGACGAT10.8403361344537815No Hit
GGGCTAGGTTTAGCTCAGAGCGGTCAAGTTAAGTTGAAATCTCCTAAGTG10.8403361344537815No Hit
GCGCCGAGGCGGTGCGCTCGCTCGCGGCGGGCGCCCCCCTGTCTCCTTTT10.8403361344537815No Hit
CCCCAAGTGTTATGGGCCCGGAGCGAGGAGAGTAGCACCTGTCTCTTATA10.8403361344537815No Hit
CGCCAAGACCGAGCCGAAGACCGCCGCCCGGGCCGAGCCGAAGGCCTCCG10.8403361344537815No Hit
CTGCTGGGCGTGGACGAGGTCTACGCGGCCGGCGGCGCCCAGGCCCTGTC10.8403361344537815No Hit
TCTCCGGCCCCACCAGCTCGGCCCAGCGGGTGAGCACGGAGCCGACCCTG10.8403361344537815No Hit
GTACAGGGCGGTGCGCTCGGACTGGCGCACCGGCTCGGCGTCCCAATCCA10.8403361344537815No Hit
GTCTCCAGGGGCACCCCCTGGCGGGCGCGCTCGACGCCGGTGGCCCGCCA10.8403361344537815No Hit
TACTTCAGGAACACCAGGCCCAGCACCACGTCCTTGTACCTGTCTCTTAT10.8403361344537815No Hit
TGCTCACTCAGAAGGACGCTGCCACCCACTGGCAGTGTGACCTCTGTCTC10.8403361344537815No Hit
GCCCACGGAGCCGGGCTTGCGGTGGTTCTTGTGCTGGCCGTGCGAGGCGC10.8403361344537815No Hit
GGCCCACCCCGCGCCGAAGGCCAGTGCCAGCGCCGCCAGGGTGGCCACGG10.8403361344537815No Hit
CACCTGGCGCACCGCGGCCACGTGGCCGGCGTCGCGGGAGGCGAGCAGGG10.8403361344537815No Hit
CCGTGGGCGGTCTCACCATGGGCGCCGACCCCGTGGCCACCGCGATGATG10.8403361344537815No Hit
CCCTATTACTATCCATCCTCATCCTAGCAATAATCCCCATCCTCCATATA10.8403361344537815No Hit
CGTCACCGTGGTGGCGTTCGAGATCGCGGAGGGCACGGCGGAGGAGCTGG10.8403361344537815No Hit
GGCCATGGCCGCCGCGGGCGTCACGAAGGTCGACGCCGCGCTCTACGACC10.8403361344537815No Hit
CATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCCGTCTTCTGCT10.8403361344537815RNA PCR Primer, Index 34 (96% over 28bp)
CCACTGTAGGGTGCGCCGCCGCCTACCCTGGATCGCATGCCGTCTCGTCC10.8403361344537815No Hit
TCTCCGAGCCCACGAGACATGATGATATCTCGTATGCCGTCTTCTGCTTG10.8403361344537815TruSeq Adapter, Index 25 (96% over 29bp)
CTTATACACATCTCCGAGCACGAGACAATTCGTTATCTCGTATGCCGTCT10.8403361344537815No Hit
TCTATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCCGT10.8403361344537815No Hit
GTTGTACTCTGTTACTTGAGGACACACATCTCCGAGCCCACGAACTAACA10.8403361344537815No Hit
AAACACCCCTCCCCACATCAAGCCCGAATGATATTTCCTATTCGCCTACA10.8403361344537815No Hit
AGCCCACGAGACGGTAAACCATCTCGTATGCCGTCTTCTGCTTGAAAAAA10.8403361344537815RNA PCR Primer, Index 36 (96% over 28bp)
CTTATACACATCTCCGAGCCCACGAGATGTGTGGTGATCTCGTATGCCGT10.8403361344537815No Hit
GGCTCGTCGACCAGGCCGAGCTCGCAGACCTGGCGGACGACGTGCTGGGC10.8403361344537815No Hit
CAGCCGCAGCCCGGCAACCCGCAGCCCCGACTGTTCCGTCTCTGTCTCTT10.8403361344537815No Hit
CTCTTATACACATCTCCGACCCACGAGACTAACAACAATCTCGTATGCCG10.8403361344537815No Hit
GTTAGCGGTTAGGCGTACGGCCAGGGCTATTGGTTGAATGAGTAGGCTGA10.8403361344537815No Hit
GGATAGGCTCGGTCCATGTCCGAGACCGCGCCCGCTGCACCTGTCTCTTA10.8403361344537815No Hit
GTCTGGGCCACCAGGAGACCGGCGTCTCCGCCGTAGAACCTGTCTCTTAT10.8403361344537815No Hit
GCACCACGGCATCCCGGTTCGGGAAGATGCCCACCGCGTCCGTGCGGCGC10.8403361344537815No Hit
TCCGTGCTCTCCACGACCAGCTCCTGGTCGCGGCAGATGAAGTGCAGGTG10.8403361344537815No Hit
GTGCTGCGGGTGTCCTCGTGCCAGTGGCGGGGGGCCTGGCTCCCCCCGTC10.8403361344537815No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGAGGTAAACCATCTCGTAT10.8403361344537815No Hit
GCCCGCGGCCAGCGCGGGCGCCAGCTTCCACAACCCCATCAGCAGCGGGA10.8403361344537815No Hit
GTGTTCGAGCACGCCGACGCCGAGGCCGACGCTCCCGGTGACGTGCCGCT10.8403361344537815No Hit
CCCTGGGAGCCGGTGACCATCAGCACGATCTCGTCGTCGCGGTACTTGTC10.8403361344537815No Hit
AGCCTGCAGAGTGGAGTGGGCCGGTCCCTCCTGCCAGGCAAAGGAAGGGG10.8403361344537815No Hit
GCCAAACATTTATTGAGTGCCTTTGTTACTGGAAAGGGGTCTTGTCCCAG10.8403361344537815No Hit
CCTCGGAGGCGGCCACGAGGTGCCCGTGGTGGATCGCTGTCTCTTATACA10.8403361344537815No Hit
TCGTCCTCCACCTCGACGCCGGCCCCGCCGCGGCCGGCGACGACCTCGCC10.8403361344537815No Hit
CAGTGGGGACCCAGGGGGCGGCGGACCGCTGCGCCCCGTCCCGCACCTGT10.8403361344537815No Hit
CTCTAGATAAGCTCCTCATGACTCATGCAACCTTTTCATCCTGTCTCTTA10.8403361344537815No Hit
TACTTTTCCCTTTCCTTTTCTCAAGTAGAAATGTTCTTCCCTCTTAGCGA10.8403361344537815No Hit
CTCGCGTGCCGTGGCCAGGAGCTCGGCCAGTTCCGCGGCTGTCTCTTATA10.8403361344537815No Hit
GCTTCATACACCAGGTTAGCTGATTTGAAAGTGTCATGCTACGTGGGGAC10.8403361344537815No Hit
ACTCCACCTCAATCACACTACTCCCCATATCTAACAACGTAAAAATAAAA10.8403361344537815No Hit
ATGGTACTGAACCTACGAGTACACCGACTACGGCGGACTAATCTTCAACT10.8403361344537815No Hit
GATCTAAAACACTCTTTACGCCGGCTTCTATTGACTTGGGTTAATCGTGT10.8403361344537815No Hit
ATTAATCGCCCCTGCCCCACCCCACCCTAATCTTCTGTCCTGTCTCTTAT10.8403361344537815No Hit
ACCCTCGACCCCGTCCGGAGCGACCCATGAGTCTCATCTGTCTCTTATAC10.8403361344537815No Hit
CCTCACGCCCGCCACCCCGGTGACGGACGTGTGGCGGATCCAGGGCTCCG10.8403361344537815No Hit
GCCCAAAGGTGGCGATGATCTTCGCGTGTCGCATGCGACCCACGATAGGG10.8403361344537815No Hit
CTGCTGCCGCTGCTGTCCCTCGGCGCCGCGGGCCTGATGCTGGCCACCGC10.8403361344537815No Hit
CTCTTCCCTGACTTATAGGCTTTGACACTGCTGTTGAGGTTTGACTCGAG10.8403361344537815No Hit
CCCCTGGACCGAGGCCGCCTCGTCCAGCAGGGGCACGTCGGCCTCGGTCC10.8403361344537815No Hit
GCTGTGACTAGTATGTTGAGTCCTGTAAGTAGGAGAGTGATATTTGATCA10.8403361344537815No Hit
GCAGAAGACGACGGCGTGCACCTTCGGCGGCGACGACCTCGGCACCCTCT10.8403361344537815No Hit
ATAATAAACACCCTCACCACTACAATCTTCCTAGGAACAACATATGACGC10.8403361344537815No Hit
GCGCGGGCGTCCATCACGCCCTCGAGGCTGTCTCTTATACACATCTCCGA10.8403361344537815No Hit
ACCGCGCACGGCGTGGTGGAGCGCCCGATCGTGCCCGTGGACGTCTGTCT10.8403361344537815No Hit
TTATAGTAGTGTGCATGGTTATTACTTTTATTTGGAGTTGCACCAAAATT10.8403361344537815No Hit
CTATAGTGGAGGCCGGAACAGGAACAGGTTGAACAGTCTACCCTCCCTTA10.8403361344537815No Hit
TCCCCGGGCCGTCCGCGGCGGAAGATGACCGACGCCCGGCGGCAGCGCCT10.8403361344537815No Hit
CCTCAAGCCTTGCAAGCAGTGGGCTGCCCACTCCCCCTATCTCCTGCTGG10.8403361344537815No Hit
GATGAGCACGGTGCGGCCGGCGACGCCGGCCTCGAGCAGATCGGTGAGGG10.8403361344537815No Hit
GCCCAGCAGCACGCCGCCCAGCGCCGCGGCGCGGCCCCCGGCCGCCCGGG10.8403361344537815No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCCAACACGATCTCGTATGCC10.8403361344537815No Hit
GTGCCGGACTGGCCCACCCGCACGACGCAGATCGGCGACCTGTCTCTTAT10.8403361344537815No Hit
GCCCGAGGTCCCGTCCATCACCACCGTGATCGGCCTGTCTCTTATACACA10.8403361344537815No Hit
TCCGCGGCGCGGGCCGTGGCGAGGTCGGCCCGCGCGTCCTCGTGGGCCTG10.8403361344537815No Hit
CCTGGTAGGATAAGAATATAAACTTCAGGGTGACCGAAAAATCAGAATAG10.8403361344537815No Hit
GTGCGTGGGCGACTGCGGGTGGGATGCTTCGCCGGCGGTGGCGACCAGCA10.8403361344537815No Hit
GGAGATGGGGTACGTCAACGCGGGCACCGTCGAGTTCCTCGTGCTGTCTC10.8403361344537815No Hit
TCCCAGCACTTTGGGAGGCCGAGGCGGGGGGGTCACGAGGTCAAAAGATC10.8403361344537815No Hit
GGTTTTCGGTCTCCGTGGGCAGAAGCGCCCTCTCTTCCTGTCTCTTATAC10.8403361344537815No Hit
AGAAGGGCATTACATAATGGTAAAGACATCAATGCAACAAGAAGAGCTAA10.8403361344537815No Hit
GCCTGGATCAAGTCGCCCCACATCATCGGCGGCCAGGCGCCCCTCCTGCG10.8403361344537815No Hit
ATCTTCGAGCCCACGAGACAAACGGTCATCTCGTATGCCGTCTTCTGCTT10.8403361344537815Illumina PCR Primer Index 7 (96% over 27bp)
GGCGTGGACTGCGGCACGAACTCCATCCGCCTGCTGATCTCCCGGCCCTG10.8403361344537815No Hit
GCCCACAGACACTTCTAGAACACGTTTTAGAAATGCTTTTTTTTCTATAA10.8403361344537815No Hit
CGCGTGGCGATCGCCCGGGCGCTCGCGCTGAACCCCGAGCTGATCGCTGT10.8403361344537815No Hit
ATCTCCGAGCCCAGAGACCCAACACGATCTCGTATGCCGTCTTCTGCTTG10.8403361344537815Illumina PCR Primer Index 1 (96% over 31bp)
GTCAAGGAGACCGTCGCCCACGGCGGCACCATCCTGTTCGTCGGCACCAA10.8403361344537815No Hit
CTCCGCGGACCAGCGGCGGCCGGCCAGGTCCTTGAGGCTGTCTCTTATAC10.8403361344537815No Hit
TTTTCGCTTCCCACAGGTCTCCGAGCCCACGAGACATGATGATATCTCGT10.8403361344537815No Hit
CTGCTGGACGGGGACGTGATCTCCGCGCCGCAGTCCAACGTGGTCATCAC10.8403361344537815No Hit

[FAIL]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers