FastQCFastQC Report
Wed 25 May 2016
SRR1780566_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780566_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30074
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTCGTC207.490901E-570.578861
TTCGTCC207.808439E-569.9916842
TCGTCCT207.808439E-569.9916843
GTACCGT150.002204861569.9916810
GTCATTG308.189805E-658.32639756
TGTTTTG308.189805E-658.32639734
CGTCCTT252.3550165E-455.9933434
GTTGTGT252.3550165E-455.99334315
TGATGGG200.006889902552.4937666
CCACTGA200.006889902552.4937635
GTAAGTA200.006889902552.4937616
CTGCCAC200.006889902552.4937626
TGAGTCC200.006889902552.493768
TTTTGAG305.791372E-446.66111836
TTAGTGT305.791372E-446.66111810
TTTGATT305.791372E-446.66111848
GGTTATT404.4915083E-543.744824
TCATTGT404.4915083E-543.744857
TTGATTA350.00123708139.99524749
TTATTTG458.9960624E-538.88426629
Produced by FastQC (version 0.11.5)