FastQCFastQC Report
Wed 25 May 2016
SRR1780566_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780566_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30074
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGT5231.7390436922258428No Hit
ATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGTCTT4071.3533284564740309RNA PCR Primer, Index 47 (95% over 21bp)
ATAAAGAATATTGAGGCGCCATTGGCGTGAAGGTAGCGGATGATTCAGCC670.22278379996009842No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCC620.20615814324665824No Hit
CTTTACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGTC480.15960630444902574No Hit
TCTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCG450.14963091042096163No Hit
TATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGTCT340.11305446565139322No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGTTCA150.002203822170.09
TACAGTT150.002203822170.07
TCGGATA150.002203822170.02
AGTTGTT150.002203822170.020
ATACAGT150.002203822170.06
GTCGGAT150.002203822170.01
ACAGTTC150.002203822170.08
AGCTTCT200.006886665752.534
GATACAG200.006886665752.55
AGCTACC200.006886665752.520
TCGGACT200.006886665752.53
CGGATAC200.006886665752.53
GGATACA200.006886665752.54
CCAAGTG200.006886665752.528
CAAGTGT200.006886665752.529
GGGCCTA200.006886665752.550
ATGTGCT305.7879795E-446.6666647
TAGCTAC350.001236358240.019
ACTCCTG400.002382260535.063
TACCCCC501.6712454E-435.023
Produced by FastQC (version 0.11.5)