##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780566_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 30074 Sequences flagged as poor quality 0 Sequence length 76 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.93133603777349 34.0 34.0 34.0 31.0 34.0 2 33.33829886280508 34.0 34.0 34.0 33.0 34.0 3 33.50957637826694 34.0 34.0 34.0 34.0 34.0 4 33.50781405865532 34.0 34.0 34.0 34.0 34.0 5 33.51489658841524 34.0 34.0 34.0 34.0 34.0 6 37.24256833144909 38.0 37.0 38.0 36.0 38.0 7 36.851466382922126 38.0 37.0 38.0 35.0 38.0 8 36.85492451951852 38.0 37.0 38.0 35.0 38.0 9 36.65917403737448 38.0 37.0 38.0 34.0 38.0 10-11 36.336287158342756 38.0 37.0 38.0 33.0 38.0 12-13 36.237813393629054 38.0 37.0 38.0 34.0 38.0 14-15 36.489076943539274 38.0 37.0 38.0 34.0 38.0 16-17 36.59027731595398 38.0 37.0 38.0 34.0 38.0 18-19 36.59323668284897 38.0 37.0 38.0 34.0 38.0 20-21 36.29731661900645 38.0 37.0 38.0 34.0 38.0 22-23 36.410337833344414 38.0 37.0 38.0 34.0 38.0 24-25 35.703980182217194 38.0 36.5 38.0 31.5 38.0 26-27 35.92762851632639 38.0 37.0 38.0 33.0 38.0 28-29 35.75435592205892 38.0 36.5 38.0 31.5 38.0 30-31 35.445966615681314 38.0 36.0 38.0 29.0 38.0 32-33 36.043210081798236 38.0 37.0 38.0 33.5 38.0 34-35 36.022594267473565 38.0 36.5 38.0 33.0 38.0 36-37 36.19442042960697 38.0 37.0 38.0 34.0 38.0 38-39 35.771746358981176 38.0 36.5 38.0 32.5 38.0 40-41 35.306843120303256 38.0 36.0 38.0 29.0 38.0 42-43 35.77645141983108 38.0 36.5 38.0 32.5 38.0 44-45 35.95792046285828 38.0 37.0 38.0 33.0 38.0 46-47 36.26700804681785 38.0 37.0 38.0 34.0 38.0 48-49 36.07912150029926 38.0 37.0 38.0 33.5 38.0 50-51 36.205409988694555 38.0 37.0 38.0 34.0 38.0 52-53 36.07922125423954 38.0 37.0 38.0 34.0 38.0 54-55 36.29798164527499 38.0 37.0 38.0 34.0 38.0 56-57 36.37312961361974 38.0 37.0 38.0 34.0 38.0 58-59 36.399448028197114 38.0 37.0 38.0 34.0 38.0 60-61 36.1251911950522 38.0 37.0 38.0 33.0 38.0 62-63 36.36099288421893 38.0 37.0 38.0 34.0 38.0 64-65 36.35226441444437 38.0 37.0 38.0 34.0 38.0 66-67 35.687204894593336 38.0 36.5 38.0 31.0 38.0 68-69 34.93772028995146 38.0 35.5 38.0 27.0 38.0 70-71 34.85962958036842 38.0 36.0 38.0 26.0 38.0 72-73 34.52571989093569 38.0 35.5 38.0 24.5 38.0 74-75 35.069678127286025 38.0 36.0 38.0 27.0 38.0 76 34.595331515594864 37.0 35.0 38.0 25.0 38.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.8009329819121831 1101 2 0.5850563916326337 1101 3 0.3579824842476995 1101 4 0.27680843154472257 1101 5 0.03812112080559871 1101 6 -1.0372604939782946 1101 7 0.274331473423608 1101 8 0.7626954067216616 1101 9 0.29239092435420133 1101 10-11 0.38613348484677346 1101 12-13 0.7265860678439182 1101 14-15 0.5614799526626939 1101 16-17 -0.13643834462436644 1101 18-19 -0.26084000872954505 1101 20-21 0.17635236057106596 1101 22-23 0.6700250853126732 1101 24-25 -1.1297539086354291 1101 26-27 -0.23398223568257492 1101 28-29 1.2944026850753048 1101 30-31 1.5732348642845864 1101 32-33 0.42962101943406594 1101 34-35 0.3566725804861903 1101 36-37 0.8059793874561336 1101 38-39 0.4110023581158373 1101 40-41 1.1702242683665602 1101 42-43 0.8546355891120854 1101 44-45 0.7651081653849658 1101 46-47 0.761297024052169 1101 48-49 -1.1226900754699685 1101 50-51 -1.690693101035457 1101 52-53 -1.2054625160498205 1101 54-55 -0.2776956883543207 1101 56-57 -0.18166403400550735 1101 58-59 -0.8465977179475672 1101 60-61 -0.7522517868514029 1101 62-63 0.14361977346307953 1101 64-65 -1.0324804327147916 1101 66-67 -1.088962407980489 1101 68-69 -0.9836594306176849 1101 70-71 -0.9711537608844125 1101 72-73 -1.1620479231721461 1101 74-75 -0.28617128378044754 1101 76 -0.40531482278410635 1102 1 0.18182840228129038 1102 2 0.45706596101062047 1102 3 0.529547761076131 1102 4 0.25425204056728035 1102 5 -0.26758632963869644 1102 6 0.2595611054748872 1102 7 -0.012408102789656539 1102 8 0.6654636910688936 1102 9 0.9687408901778554 1102 10-11 0.9617486591461599 1102 12-13 0.4818834020886271 1102 14-15 0.11940886585477273 1102 16-17 0.10527389050891856 1102 18-19 -0.8864380948539434 1102 20-21 0.44489303042752937 1102 22-23 0.13106404635163926 1102 24-25 -5.649148987241027 1102 26-27 -1.157854416133702 1102 28-29 -0.009254184371037866 1102 30-31 1.5874180495204016 1102 32-33 0.9510666790376234 1102 34-35 1.243634302974229 1102 36-37 -2.4454731074174134 1102 38-39 0.054030382722807246 1102 40-41 0.8951046511416791 1102 42-43 1.0815665528851568 1102 44-45 1.4780268256720461 1102 46-47 1.275223886663241 1102 48-49 -3.757942980459717 1102 50-51 -0.9628222876383319 1102 52-53 -0.42308042445719707 1102 54-55 0.4118121722061616 1102 56-57 0.7259743802118592 1102 58-59 0.4990106210818226 1102 60-61 -5.294630460809024 1102 62-63 -2.382354252510943 1102 64-65 0.16540063358733192 1102 66-67 1.1392501689162913 1102 68-69 -1.4544386513969059 1102 70-71 -0.7255966863799657 1102 72-73 -5.402563986056627 1102 74-75 -2.3509354669656872 1102 76 0.048717986511860545 1103 1 0.6854359058887951 1103 2 0.5692994065774002 1103 3 0.1487557136174189 1103 4 0.46589225220748887 1103 5 -0.13098219303455494 1103 6 0.10195328120039449 1103 7 0.676865586484034 1103 8 0.5351125607177636 1103 9 0.5441777989480983 1103 10-11 0.08504511577594798 1103 12-13 0.06349315036504066 1103 14-15 0.26274700919291405 1103 16-17 0.26552721742891805 1103 18-19 0.9137863720371939 1103 20-21 -0.3744111222099562 1103 22-23 0.08360533222625577 1103 24-25 -2.9811996526250297 1103 26-27 -0.7054734637527531 1103 28-29 -0.6921145338980565 1103 30-31 1.041883337319021 1103 32-33 1.327850389154662 1103 34-35 -0.05715133114473758 1103 36-37 -0.8768503721280112 1103 38-39 0.34391333927243295 1103 40-41 -0.16830724560355037 1103 42-43 -0.8198283485097448 1103 44-45 1.122807970453188 1103 46-47 0.6176160623887483 1103 48-49 -2.3519785744953126 1103 50-51 -0.14464046945651177 1103 52-53 0.29721779584102137 1103 54-55 -0.42015832643100026 1103 56-57 0.0027901570276327448 1103 58-59 -0.44463215589428273 1103 60-61 -3.2684961013413343 1103 62-63 -0.6032938067838316 1103 64-65 0.6920159935360246 1103 66-67 -0.28819892519946677 1103 68-69 0.8157232854316945 1103 70-71 0.5725434450935012 1103 72-73 -5.027463777623083 1103 74-75 -1.6495565989201495 1103 76 -3.802813198352659 1104 1 0.11783215756215526 1104 2 0.32015467952102483 1104 3 0.35164133015765486 1104 4 -0.12268427612807642 1104 5 -0.18809955135673562 1104 6 0.08306290488030044 1104 7 0.13514314114713244 1104 8 0.4204316147115179 1104 9 0.9496515457873045 1104 10-11 -0.7312571302451758 1104 12-13 0.47484225046915896 1104 14-15 0.8533315696569872 1104 16-17 0.3729941220845703 1104 18-19 0.7789507331854821 1104 20-21 -0.19420883156590207 1104 22-23 0.3085793021801493 1104 24-25 0.4370660025884163 1104 26-27 1.061594809339617 1104 28-29 1.7622213280683283 1104 30-31 1.2677497659966974 1104 32-33 0.5146717486667853 1104 34-35 0.09248424820971479 1104 36-37 0.9441372821929761 1104 38-39 1.3639192889972591 1104 40-41 2.0763751878339036 1104 42-43 1.1596451008432211 1104 44-45 0.8285197062854053 1104 46-47 0.9601714692011853 1104 48-49 0.3544025053797455 1104 50-51 1.0180101329531297 1104 52-53 1.1042235968227274 1104 54-55 0.9891083699842937 1104 56-57 0.5619155474783213 1104 58-59 -0.855237540395251 1104 60-61 0.5749517641948856 1104 62-63 0.3183185686438037 1104 64-65 0.8549640821869176 1104 66-67 1.037622880542024 1104 68-69 2.139834545285929 1104 70-71 1.8524036265601111 1104 72-73 2.035082704602111 1104 74-75 0.7932516711973605 1104 76 0.4467777580656076 1105 1 1.1066245363797478 1105 2 0.5437467221099865 1105 3 0.10339252096740381 1105 4 -0.16933599929983245 1105 5 -0.22228279596306777 1105 6 0.10870033833504777 1105 7 0.8116631747699969 1105 8 -0.2939141404019594 1105 9 0.7783150369613026 1105 10-11 0.10966469659707911 1105 12-13 -1.1678447767093232 1105 14-15 -0.9024123361989922 1105 16-17 -0.1525032161053943 1105 18-19 0.8878748015519733 1105 20-21 -0.02973699071412028 1105 22-23 -0.2369516588733731 1105 24-25 0.6326389922213664 1105 26-27 -0.5872564094891857 1105 28-29 0.12212606328827036 1105 30-31 1.2699912902331718 1105 32-33 0.9181461112333338 1105 34-35 -1.056577113516262 1105 36-37 0.24646286358832725 1105 38-39 -1.3374676541240618 1105 40-41 0.48601374205077263 1105 42-43 0.1422732880104931 1105 44-45 0.6772868679553383 1105 46-47 1.1825026544071235 1105 48-49 -0.46158236433166167 1105 50-51 0.5995455770551175 1105 52-53 0.20656318774455684 1105 54-55 0.7969677143384573 1105 56-57 -0.3516456741523868 1105 58-59 0.23036066638535146 1105 60-61 1.1299391556210523 1105 62-63 -0.23428720328110586 1105 64-65 0.6578500446413855 1105 66-67 -0.3828730566953027 1105 68-69 -0.5999385003851287 1105 70-71 0.5995316736321143 1105 72-73 -0.7515522071378697 1105 74-75 -2.331152923927327 1105 76 -1.0378117628085874 1106 1 -1.6108603904075025 1106 2 0.03843520904653275 1106 3 0.39566929386433003 1106 4 -0.17608305643448574 1106 5 0.029511634125938713 1106 6 0.3982495774966921 1106 7 -0.9280726851209025 1106 8 0.36227305454492154 1106 9 -0.6780444232741232 1106 10-11 0.07887227626977023 1106 12-13 1.3227524096243002 1106 14-15 -0.5520678056219026 1106 16-17 -0.0986703134352851 1106 18-19 0.4261320510495352 1106 20-21 0.06386048272830891 1106 22-23 0.9354571840781034 1106 24-25 -0.5614465662052766 1106 26-27 0.8500820918028111 1106 28-29 0.3757867006698419 1106 30-31 -0.1679932058908591 1106 32-33 -0.7215323268947174 1106 34-35 0.3626017552750085 1106 36-37 1.2466064179954444 1106 38-39 0.6772466726057687 1106 40-41 1.115643371680406 1106 42-43 0.38387535519395755 1106 44-45 0.5919437729223276 1106 46-47 0.6237889018949261 1106 48-49 0.6295029069861755 1106 50-51 -0.3298256546417022 1106 52-53 0.6675881662113952 1106 54-55 -0.11151635112235425 1106 56-57 0.08920991011405022 1106 58-59 0.2775900663279316 1106 60-61 0.3981705653253371 1106 62-63 0.5633728598828327 1106 64-65 -1.4067494385627484 1106 66-67 0.5945171241832554 1106 68-69 1.266340569382315 1106 70-71 -0.49535778947440434 1106 72-73 -0.015118098610741981 1106 74-75 -0.3038775865744654 1106 76 0.12311272757326464 1107 1 0.7926710480076551 1107 2 -0.09578816161094039 1107 3 0.42897391631624515 1107 4 0.3422919076769162 1107 5 0.14981022729274684 1107 6 0.6435840592652795 1107 7 0.5442213143048775 1107 8 0.13631841773757714 1107 9 -0.48912682350381687 1107 10-11 0.749199580318006 1107 12-13 7.598744534718094E-4 1107 14-15 0.06270394287078318 1107 16-17 0.5219153885457715 1107 18-19 0.8936887550403441 1107 20-21 0.8365420790533236 1107 22-23 -0.22532375189663156 1107 24-25 0.10938317826788335 1107 26-27 0.03483661376662894 1107 28-29 1.3721260632882775 1107 30-31 1.3455726855820132 1107 32-33 1.0983786693728632 1107 34-35 0.36202753764653295 1107 36-37 0.97320704963483 1107 38-39 0.6102067644805871 1107 40-41 0.6895021141437923 1107 42-43 0.8922732880104931 1107 44-45 0.7703101237692991 1107 46-47 1.1243631195234016 1107 48-49 0.49190600776136506 1107 50-51 0.9309409258923225 1107 52-53 0.45074923425618607 1107 54-55 0.36092120271054995 1107 56-57 0.9390520002662228 1107 58-59 0.9861746198737293 1107 60-61 1.1531949695745496 1107 62-63 0.6378058199747088 1107 64-65 1.2741291144088294 1107 66-67 0.37875485028144595 1107 68-69 1.5686661507776662 1107 70-71 0.5762758596786242 1107 72-73 1.2368198858853816 1107 74-75 -0.6683622262529028 1107 76 0.4970719581216443 1108 1 -0.39885528185067187 1108 2 -0.08629872028509311 1108 3 -0.37401993559501534 1108 4 0.47100080904018427 1108 5 0.16531410326173557 1108 6 -0.5613076809111419 1108 7 -0.29886876856066635 1108 8 -1.2298938249782765 1108 9 -0.8470525118224472 1108 10-11 0.1452835150948104 1108 12-13 1.0971244828469437 1108 14-15 0.14269592362458638 1108 16-17 -0.7027571589014414 1108 18-19 0.395693566588065 1108 20-21 0.997662100437978 1108 22-23 0.187266464623022 1108 24-25 0.8768250387329957 1108 26-27 -0.6709907051320627 1108 28-29 -1.5814291358563324 1108 30-31 0.021661966522934506 1108 32-33 1.052201176723841 1108 34-35 0.23318498218007733 1108 36-37 0.3694246385148219 1108 38-39 0.7459993333124473 1108 40-41 0.38256546618870857 1108 42-43 -0.1716802003615996 1108 44-45 0.6828335132373553 1108 46-47 0.9313665945300897 1108 48-49 0.727204056411459 1108 50-51 0.372600909853837 1108 52-53 -0.398823072613645 1108 54-55 -1.0361651378401078 1108 56-57 -0.7877590128318843 1108 58-59 0.17903749076386788 1108 60-61 1.162751237951987 1108 62-63 0.7355737964515825 1108 64-65 -0.2956383274516341 1108 66-67 0.5466244038767343 1108 68-69 0.7376049371984053 1108 70-71 -1.4368222424331023 1108 72-73 -1.0036238457371738 1108 74-75 -1.8707145384688957 1108 76 -1.088466667914176 1109 1 -1.1668229463700577 1109 2 -0.08339357944891646 1109 3 0.3816956132239824 1109 4 0.13337291968815634 1109 5 0.22392216186979397 1109 6 -0.5336456877319051 1109 7 -0.0573631477447023 1109 8 0.31980934541454786 1109 9 0.5032064246433876 1109 10-11 -0.18160798421048696 1109 12-13 0.24162366182888917 1109 14-15 0.6428853893312976 1109 16-17 0.6622169474519737 1109 18-19 0.8098656014497507 1109 20-21 0.6836542691887644 1109 22-23 0.22497014025772444 1109 24-25 -0.6616364997285444 1109 26-27 -0.020491778771067004 1109 28-29 -0.9772861524030105 1109 30-31 0.538966501068856 1109 32-33 0.1468708748418166 1109 34-35 -0.17145061211068935 1109 36-37 1.108972447028144 1109 38-39 0.4516327803252054 1109 40-41 -0.29420603816900837 1109 42-43 0.4821659534845608 1109 44-45 0.4565483041935252 1109 46-47 0.7127863242256751 1109 48-49 0.484814262297526 1109 50-51 -0.32595915077519777 1109 52-53 -0.14336014473692416 1109 54-55 -0.4163596559656568 1109 56-57 0.16803232226980214 1109 58-59 -0.5699204478492419 1109 60-61 0.5450298788513166 1109 62-63 0.3633999932432985 1109 64-65 0.2318342000208915 1109 66-67 0.41747194713807545 1109 68-69 -0.2940989910572469 1109 70-71 0.45322449244121543 1109 72-73 -0.8740924575850997 1109 74-75 -0.31147492750513806 1109 76 0.6651016028954757 1110 1 -0.3528782703564204 1110 2 -0.33917228350348694 1110 3 -0.08666361375593112 1110 4 0.17215023432753895 1110 5 0.4296819193536905 1110 6 0.5191520892038 1110 7 0.551705944083011 1110 8 1.0344739911136784 1110 9 0.808119901970656 1110 10-11 -0.09609579525002232 1110 12-13 1.0971244828469437 1110 14-15 1.0737304063832056 1110 16-17 0.555863530753733 1110 18-19 0.010636095323690142 1110 20-21 1.1873172728517645 1110 22-23 -0.5311243399746814 1110 24-25 -0.6059335819566556 1110 26-27 1.426710444293228 1110 28-29 0.016272013568944033 1110 30-31 0.12511024238500568 1110 32-33 0.8050747399422278 1110 34-35 0.7216907293065162 1110 36-37 1.3234476270205633 1110 38-39 0.8896774942319894 1110 40-41 -0.7208828096733697 1110 42-43 0.4834922134315036 1110 44-45 1.0793852373752841 1110 46-47 0.4715964795875607 1110 48-49 1.2444454357218007 1110 50-51 0.11398022019866261 1110 52-53 0.2103723296852067 1110 54-55 -0.28329157462172105 1110 56-57 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2116 50-51 -2.267928140689385 2116 52-53 -0.3456031072538579 2116 54-55 -0.7814808361553318 2116 56-57 -0.5195335365289395 2116 58-59 -2.7758515030953674 2116 60-61 -2.0291083117252313 2116 62-63 0.6367704583945866 2116 64-65 -1.5970081904653313 2116 66-67 -0.6809775485716969 2116 68-69 -2.219960800480699 2116 70-71 0.35454919513578176 2116 72-73 -0.15438750500343446 2116 74-75 0.3571235972577611 2116 76 -1.253007685076824 2117 1 0.8970036380044704 2117 2 0.6886973433269361 2117 3 0.4726183890782707 2117 4 0.34818550436375517 2117 5 0.34796250508821913 2117 6 0.5851259515876777 2117 7 0.7844252008929615 2117 8 0.6731135754311097 2117 9 -0.0807165017452931 2117 10-11 0.14000875521447398 2117 12-13 -0.8346972775057537 2117 14-15 0.77543092598642 2117 16-17 -0.5656921297721667 2117 18-19 -1.472988530719448 2117 20-21 0.21699763814857675 2117 22-23 0.5193401538058211 2117 24-25 -1.3286544133218001 2117 26-27 -1.4885784865837977 2117 28-29 -0.16251872804654255 2117 30-31 0.014655195935631582 2117 32-33 -1.6176933280785448 2117 34-35 0.6801224723391073 2117 36-37 -0.6859997041083901 2117 38-39 1.1296397049074685 2117 40-41 1.09560278314666 2117 42-43 0.4584567859397666 2117 44-45 -0.2305659514138867 2117 46-47 -0.4266085274979403 2117 48-49 -0.2083966363928269 2117 50-51 0.5265924072558192 2117 52-53 1.1064516872666914 2117 54-55 0.19797121863918932 2117 56-57 0.8229322168957154 2117 58-59 -0.46078300994468435 2117 60-61 -0.17979324323207635 2117 62-63 0.10252388305212179 2117 64-65 -1.2134465466297186 2117 66-67 -0.37960768555799973 2117 68-69 1.0608611173275264 2117 70-71 0.5942752225330352 2117 72-73 2.0373933169143754 2117 74-75 1.37767154246324 2117 76 1.3360334108135845 2118 1 -0.17275122861413195 2118 2 -0.033364660998948636 2118 3 -1.2999122525944102 2118 4 0.14522948417630488 2118 5 -0.10661572129966856 2118 6 -0.36620786239934233 2118 7 0.23485778921308054 2118 8 0.6311164593532439 2118 9 -1.6681353914352712 2118 10-11 -1.377024409890069 2118 12-13 -2.2470981426823897 2118 14-15 0.2456904789784886 2118 16-17 -4.971964660413839 2118 18-19 -1.6161031666242849 2118 20-21 -1.3894371131131393 2118 22-23 0.018709295838988282 2118 24-25 0.33077240715405054 2118 26-27 -4.089245393577315 2118 28-29 -1.7253341570299625 2118 30-31 -1.966238819925941 2118 32-33 -1.3532737174080367 2118 34-35 -0.9064853142506522 2118 36-37 -4.134810086228079 2118 38-39 -0.6416292208315326 2118 40-41 -1.7113546790018574 2118 42-43 0.6506882206910305 2118 44-45 -3.9783128872466165 2118 46-47 -0.8571240285794133 2118 48-49 -0.7476900754699685 2118 50-51 -2.559114153667032 2118 52-53 -3.6988835686813957 2118 54-55 -2.1066430567753756 2118 56-57 -0.43166403400550735 2118 58-59 -2.228176665315985 2118 60-61 -2.100935997377718 2118 62-63 -2.4024328581158656 2118 64-65 -1.0193225379779491 2118 66-67 -0.7271203027173385 2118 68-69 -2.38497522009137 2118 70-71 -0.9579958661475629 2118 72-73 0.9761099715647035 2118 74-75 -0.5690660206225502 2118 76 -1.0368937701525311 2119 1 0.7510924715453626 2119 2 -0.8914188874742308 2119 3 0.08366031518868766 2119 4 -0.3539730676578259 2119 5 -0.3649889270412956 2119 6 -0.25775491184113264 2119 7 0.013565923184373219 2119 8 -0.042328516723316056 2119 9 -0.1286617072247438 2119 10-11 -0.04149809410059646 2119 12-13 0.5814505016557234 2119 14-15 -0.9401149436690375 2119 16-17 -0.6533841181490843 2119 18-19 0.3256831172672676 2119 20-21 -0.9945009089664083 2119 22-23 -0.3754294601418735 2119 24-25 0.2859159478239022 2119 26-27 0.3854356271563404 2119 28-29 0.4582782831614267 2119 30-31 1.2789764910788364 2119 32-33 1.1718458998168444 2119 34-35 0.5390888484287686 2119 36-37 -0.34374150568581285 2119 38-39 -1.2122033835109605 2119 40-41 -1.1200468640098364 2119 42-43 0.11619858751718937 2119 44-45 0.7810571287023507 2119 46-47 0.5457866572260102 2119 48-49 -0.09776982028655823 2119 50-51 0.8326322578162149 2119 52-53 0.3334130820363086 2119 54-55 0.0416823020763033 2119 56-57 -1.2480515938141181 2119 58-59 0.1743352964064968 2119 60-61 0.007317591139027968 2119 62-63 0.06786219770550161 2119 64-65 -0.23503226684557177 2119 66-67 -0.0620485323824056 2119 68-69 1.0011890542307995 2119 70-71 -0.09356205434533393 2119 72-73 0.5379122044195626 2119 74-75 -0.5684679344981518 2119 76 0.9794539172477883 >>END_MODULE >>Per sequence quality scores pass #Quality Count 20 1.0 21 1.0 22 3.0 23 5.0 24 13.0 25 19.0 26 60.0 27 96.0 28 170.0 29 318.0 30 455.0 31 736.0 32 1245.0 33 1793.0 34 2682.0 35 4085.0 36 7068.0 37 11324.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 34.34195650728204 24.659174037374477 11.255569594999002 29.743299860344486 2 16.259892265744497 20.050541996408857 39.988029527166326 23.70153621068032 3 16.00053202101483 26.02912815056195 32.24712376138857 25.72321606703465 4 12.216532553035844 20.529360909755937 39.88827558688568 27.36583095032254 5 11.534880627784798 38.70785396023143 36.80587883221387 12.951386579769899 6 24.955110726873713 41.53754073285894 18.341424486267208 15.165924054000133 7 24.020748819578376 31.575447230165594 23.87111790915741 20.532686041098625 8 20.74216931568797 37.82336902307642 21.33404269468644 20.10041896654918 9 21.852763184145772 15.235751812196582 22.680720888475093 40.23076411518255 10-11 23.636696149497904 26.152158010241404 28.04249517855955 22.168650661701136 12-13 23.031522245128684 22.235153288554898 27.340892465252374 27.392432001064044 14-15 19.959100884484936 24.842056261222318 24.49125490456873 30.707587949724015 16-17 22.93509343619073 28.536277182948727 24.720688967214205 23.80794041364634 18-19 22.652457272062247 27.32094167719625 25.701602713307174 24.324998337434327 20-21 21.12289685442575 26.828822238478423 25.151293476092306 26.896987431003527 22-23 21.57345215135998 28.370020615814322 26.616013832546386 23.44051340027931 24-25 22.03730797366496 28.970206823169516 25.679989359579704 23.312495843585822 26-27 21.410520715568264 30.37174968411252 25.754804814790184 22.46292478552903 28-29 21.513599787191595 28.05745826960165 27.774822105473167 22.654119837733592 30-31 21.191062046950854 28.05745826960165 28.601117244131142 22.150362439316353 32-33 23.955908758395957 27.8629380860544 26.41484338631376 21.766309769235885 34-35 21.30411651260225 27.99594333976192 29.166389572388105 21.533550575247723 36-37 22.191926581099953 27.856287823369026 26.938551572787123 23.013234022743898 38-39 21.862738578173836 27.0848573518654 27.62851632639489 23.42388774356587 40-41 20.338165857551374 25.997539402806414 29.467313958901375 24.19698078074084 42-43 22.59260490789386 25.927711644609964 29.104874642548378 22.374808804947797 44-45 19.939150096428808 26.36662898184478 29.02340892465252 24.670811997073887 46-47 21.729733324466316 25.54532154020084 28.72248453813926 24.00246059719359 48-49 20.83361042761189 25.07481545521048 29.279444038039504 24.812130079138125 50-51 19.21094633238013 25.3474762253109 30.203830551306776 25.237746891002192 52-53 19.729666821839462 25.354126487996275 31.776617676398217 23.13958901376604 54-55 18.022211877369156 26.02414045354792 30.976923588481746 24.976724080601183 56-57 20.717230830617808 25.297599255170578 28.908691893329785 25.076478020881826 58-59 18.436190729533816 27.580301921925916 29.824765578240342 24.158741770299926 60-61 20.38139256500632 28.85050209483275 27.269402141384585 23.49870319877635 62-63 18.136928908691893 30.927046618341425 26.61933896388907 24.316685509077608 64-65 17.86260557292013 31.67686373611758 25.65837600585223 24.80215468511006 66-67 18.394626587750214 31.429141451087318 25.297599255170578 24.878632705991887 68-69 18.658974529493914 30.49810467513467 25.121367294008113 25.721553501363303 70-71 18.58249650861209 30.26202034980382 24.72567666422824 26.429806477355854 72-73 18.614085256367627 29.681784930504758 24.783866462725275 26.92026335040234 74-75 19.230897120436257 29.861342023009907 24.689100219458666 26.218660637095166 76 20.35312894859347 29.523841191727072 24.729001795570927 25.394028064108532 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.5 10 0.5 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.5 17 1.0 18 1.5 19 1.5 20 2.5 21 4.0 22 5.5 23 9.5 24 12.5 25 13.0 26 40.0 27 88.0 28 132.5 29 156.0 30 184.0 31 231.0 32 323.5 33 397.0 34 476.0 35 647.5 36 818.5 37 897.0 38 1077.5 39 1508.0 40 1971.5 41 2355.5 42 2526.0 43 2433.0 44 2451.0 45 2469.0 46 2376.0 47 2315.0 48 2169.0 49 1921.0 50 1758.0 51 1634.5 52 1289.0 53 964.0 54 861.0 55 827.5 56 639.5 57 409.0 58 333.0 59 263.0 60 158.5 61 116.5 62 109.0 63 69.5 64 23.0 65 16.0 66 10.5 67 5.0 68 4.0 69 1.5 70 0.5 71 1.0 72 1.0 73 0.5 74 0.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 30074.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.995810334508214 #Duplication Level Percentage of deduplicated Percentage of total 1 62.300644723447576 30.52470572587617 2 16.851034950797423 16.512602247788788 3 8.082796063793689 11.880694287424355 4 4.7302341364099085 9.270466183414245 5 2.7417712928401765 6.716765312229834 6 1.9273837801153715 5.666023807940414 7 1.1401425178147269 3.9103544590011308 8 0.5836443841194434 2.287690363769369 9 0.4546996946046827 2.005054199640886 >10 1.160502205632847 7.70432932100818 >50 0.013573125212080081 0.42894194320675666 >100 0.0067865626060400405 1.3533284564740309 >500 0.0067865626060400405 1.7390436922258428 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGT 523 1.7390436922258428 No Hit ATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGTCTT 407 1.3533284564740309 RNA PCR Primer, Index 47 (95% over 21bp) ATAAAGAATATTGAGGCGCCATTGGCGTGAAGGTAGCGGATGATTCAGCC 67 0.22278379996009842 No Hit CTCTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCC 62 0.20615814324665824 No Hit CTTTACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGTC 48 0.15960630444902574 No Hit TCTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCG 45 0.14963091042096163 No Hit TATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGTCT 34 0.11305446565139322 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.02660105074150429 0.0 2 0.0 0.0 0.0 0.02660105074150429 0.0 3 0.0 0.0 0.0 0.02660105074150429 0.0 4 0.0 0.0 0.0 0.02660105074150429 0.0 5 0.0 0.0 0.0 0.02660105074150429 0.0 6 0.0 0.0 0.0 0.02660105074150429 0.0 7 0.0 0.0 0.0 0.02660105074150429 0.0 8 0.0 0.0 0.0 0.02660105074150429 0.0 9 0.0 0.0 0.0 0.029926182084192326 0.0 10 0.0 0.0 0.0 0.04322670745494447 0.0 11 0.0 0.0 0.0 0.04322670745494447 0.0 12 0.0 0.0 0.0 0.04322670745494447 0.0 13 0.0 0.0 0.0 0.04322670745494447 0.0 14 0.0 0.0 0.0 0.05320210148300858 0.0 15 0.0 0.0 0.0 0.05985236416838465 0.0 16 0.0 0.0 0.0 0.05985236416838465 0.0 17 0.0 0.0 0.0 0.05985236416838465 0.0 18 0.0 0.0 0.0 0.06317749551107268 0.0 19 0.0 0.0 0.0 0.08312828356720091 0.0 20 0.0 0.0 0.0 0.08312828356720091 0.0 21 0.0 0.0 0.0 0.08312828356720091 0.0 22 0.0 0.0 0.0 0.08312828356720091 0.0 23 0.0 0.0 0.0 0.08645341490988893 0.0 24 0.0 0.0 0.0 0.08645341490988893 0.0 25 0.0 0.0 0.0 0.08645341490988893 0.0 26 0.0 0.0 0.0 0.08977854625257697 0.0 27 0.0 0.0 0.0 0.1097293343087052 0.0 28 0.0 0.0 0.0 0.12968012236483342 0.0 29 0.0 0.0 0.0 0.14630577907827358 0.0 30 0.0 0.0 0.0 0.17623196116246592 0.0 31 0.0 0.0 0.0 0.23608432533085058 0.0 32 0.0 0.0 0.0 0.27266077010041895 0.0 33 0.0 0.0 0.0 0.33251313426880363 0.0 34 0.0 0.0 0.0 0.36908957903837203 0.0 35 0.0 0.0 0.0 0.42894194320675666 0.0 36 0.0 0.0 0.0 0.5453215402008379 0.0 37 0.0 0.0 0.0 0.824632572986633 0.0 38 0.0 0.0 0.0 1.203697546053069 0.0 39 0.0 0.0 0.0 1.7257431668550909 0.0 40 0.0 0.0 0.0 2.294340626454745 0.0 41 0.0 0.0 0.0 2.7964354592006386 0.0 42 0.0 0.0 0.0 3.321806211345348 0.0 43 0.0 0.0 0.0 3.9103544590011303 0.0 44 0.0 0.0 0.0 4.60530690962293 0.0 45 0.0 0.0 0.0 5.5596196049743964 0.0 46 0.0 0.0 0.0 6.201369954113187 0.0 47 0.0 0.0 0.0 6.889672142049611 0.0 48 0.0 0.0 0.0 7.591274855356787 0.0 49 0.0 0.0 0.0 8.219724679124825 0.0 50 0.0 0.0 0.0 8.918002261089313 0.0 51 0.0 0.0 0.0 9.646206025137992 0.0 52 0.0 0.0 0.0 10.258030192192592 0.0 53 0.0 0.0 0.0 10.976258562213207 0.0 54 0.0 0.0 0.0 11.641284830750815 0.0 55 0.0 0.0 0.0 12.332912150029927 0.0 56 0.0 0.0 0.0 12.931435791713772 0.0 57 0.0 0.0 0.0 13.52995943339762 0.0 58 0.0 0.0 0.0 14.042029660171577 0.0 59 0.0 0.0 0.0 14.597326594400478 0.0 60 0.0 0.0 0.0 15.122697346545188 0.0 61 0.0 0.0 0.0 15.638092704661835 0.0 62 0.0 0.0 0.0 16.27319279111525 0.0 63 0.0 0.0 0.0 16.838465119372216 0.0 64 0.0 0.0 0.0 17.43698876105606 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CAGTTCA 15 0.0022038221 70.0 9 TACAGTT 15 0.0022038221 70.0 7 TCGGATA 15 0.0022038221 70.0 2 AGTTGTT 15 0.0022038221 70.0 20 ATACAGT 15 0.0022038221 70.0 6 GTCGGAT 15 0.0022038221 70.0 1 ACAGTTC 15 0.0022038221 70.0 8 AGCTTCT 20 0.0068866657 52.5 34 GATACAG 20 0.0068866657 52.5 5 AGCTACC 20 0.0068866657 52.5 20 TCGGACT 20 0.0068866657 52.5 3 CGGATAC 20 0.0068866657 52.5 3 GGATACA 20 0.0068866657 52.5 4 CCAAGTG 20 0.0068866657 52.5 28 CAAGTGT 20 0.0068866657 52.5 29 GGGCCTA 20 0.0068866657 52.5 50 ATGTGCT 30 5.7879795E-4 46.666664 7 TAGCTAC 35 0.0012363582 40.0 19 ACTCCTG 40 0.0023822605 35.0 63 TACCCCC 50 1.6712454E-4 35.0 23 >>END_MODULE