FastQCFastQC Report
Wed 25 May 2016
SRR1780564_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780564_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57877
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCCGA150.00221486169.9948161
AGTCAAC150.00221486169.9948148
CTTCAAG150.00221486169.9948143
CCGGATA150.00221486169.9948119
TGAACTG150.00221486169.9948128
TTCAAGT150.00221486169.9948144
TCGGCTA150.00221486169.994815
ATTCCGG150.00221486169.9948116
AGGCCGA150.00221486169.9948125
ACGTCGG150.00221486169.994816
AGTTAGA150.00221486169.9948151
CGTCGGG150.00221486169.994817
CAACTAG150.00221486169.9948151
AGTAGAC150.00221486169.994812
TCAAGTC150.00221486169.9948145
AAAATCG150.00221486169.9948124
AATCGTA150.00221486169.9948126
AGTAACG150.00221486169.994812
GATTCTT252.3729085E-455.9958525
GAGTAAC200.00677987552.7697681
Produced by FastQC (version 0.11.5)