FastQCFastQC Report
Wed 25 May 2016
SRR1780564_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780564_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57877
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGT8231.4219810978454308No Hit
ATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGTCTT4270.7377714808991482Illumina PCR Primer Index 9 (95% over 22bp)
ATAAAGAATATTGAGGCGCCATTGGCGTGAAGGTAGCGGATGATTCAGCC1390.24016448675639718No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCC1060.1831470186775403No Hit
TCTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCG590.10194032171674412No Hit
CTTTACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGTC580.10021251965374846No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACTCAG150.0022142170.000017
CTAGAAC200.006918878352.524
GAGTGAA200.006918878352.528
GACAATC200.006918878352.557
ATCAAGC200.006918878352.539
TACATAC200.006918878352.53
AAGCCCG200.006918878352.542
AGTGAAG200.006918878352.529
TGACAAT200.006918878352.556
AAACCTT200.006918878352.519
CCGAATG200.006918878352.546
CAATCGA200.006918878352.559
AGGTGGG200.006918878352.57
GCCCGAA200.006918878352.544
ATCGAGT200.006918878352.561
AATCGAG200.006918878352.560
TATGCCG2250.040.44444743
ATGCCGT2250.040.44444744
TGAAAAA2100.040.00000459
CAGAGAA350.00124591140.041