##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780564_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 57877 Sequences flagged as poor quality 0 Sequence length 76 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.91214126509667 34.0 34.0 34.0 31.0 34.0 2 33.35499421186309 34.0 34.0 34.0 33.0 34.0 3 33.497555160080864 34.0 34.0 34.0 34.0 34.0 4 33.50304957064119 34.0 34.0 34.0 34.0 34.0 5 33.515178741123414 34.0 34.0 34.0 34.0 34.0 6 37.261312784007465 38.0 37.0 38.0 36.0 38.0 7 36.862346009641136 38.0 37.0 38.0 35.0 38.0 8 36.84769424814693 38.0 37.0 38.0 35.0 38.0 9 36.68068490073777 38.0 37.0 38.0 34.0 38.0 10-11 36.33246367296162 38.0 37.0 38.0 33.0 38.0 12-13 36.25062632824783 38.0 37.0 38.0 34.0 38.0 14-15 36.49441919933653 38.0 37.0 38.0 34.0 38.0 16-17 36.615961435457955 38.0 37.0 38.0 34.0 38.0 18-19 36.62505831331963 38.0 37.0 38.0 34.0 38.0 20-21 36.34085215197747 38.0 37.0 38.0 34.0 38.0 22-23 36.40424520966878 38.0 37.0 38.0 34.0 38.0 24-25 35.74958100799972 38.0 36.5 38.0 31.5 38.0 26-27 35.964277692347565 38.0 37.0 38.0 33.0 38.0 28-29 35.80488795203621 38.0 36.5 38.0 31.5 38.0 30-31 35.523238937747294 38.0 36.0 38.0 31.0 38.0 32-33 36.0572766383883 38.0 37.0 38.0 33.5 38.0 34-35 36.07076213348999 38.0 37.0 38.0 33.0 38.0 36-37 36.22761200476873 38.0 37.0 38.0 34.0 38.0 38-39 35.81688753736372 38.0 36.5 38.0 32.5 38.0 40-41 35.37408642465919 38.0 36.0 38.0 29.0 38.0 42-43 35.75743386837604 38.0 36.5 38.0 32.5 38.0 44-45 35.9855210187121 38.0 37.0 38.0 33.0 38.0 46-47 36.30012785735266 38.0 37.0 38.0 34.0 38.0 48-49 36.07241218446015 38.0 37.0 38.0 33.0 38.0 50-51 36.23008276171882 38.0 37.0 38.0 34.0 38.0 52-53 36.154370475318345 38.0 37.0 38.0 34.0 38.0 54-55 36.33805311263542 38.0 37.0 38.0 34.0 38.0 56-57 36.359235965927745 38.0 37.0 38.0 34.0 38.0 58-59 36.439492371753886 38.0 37.0 38.0 34.0 38.0 60-61 36.12922231629145 38.0 37.0 38.0 33.0 38.0 62-63 36.39311816438309 38.0 37.0 38.0 34.0 38.0 64-65 36.39456087910569 38.0 37.0 38.0 34.0 38.0 66-67 35.797812602588245 38.0 36.5 38.0 31.5 38.0 68-69 35.14865145048984 38.0 36.0 38.0 29.0 38.0 70-71 35.12474730894829 38.0 36.0 38.0 28.0 38.0 72-73 34.81445997546521 38.0 35.5 38.0 26.0 38.0 74-75 35.45734920607495 38.0 36.5 38.0 31.0 38.0 76 34.911000915735094 37.0 35.0 38.0 27.0 38.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.5545090942149571 1101 2 0.4423596116639672 1101 3 0.04675905348238274 1101 4 0.21257538730449 1101 5 -0.1925145655600602 1101 6 -1.6865750798087333 1101 7 -0.44318633656306616 1101 8 0.298834904298765 1101 9 0.059401656103560185 1101 10-11 -0.0414308550551965 1101 12-13 0.587095951095904 1101 14-15 0.5956023382673123 1101 16-17 -0.1799215893042927 1101 18-19 -0.5715977798616478 1101 20-21 -0.4826407345672479 1101 22-23 0.3248385905208906 1101 24-25 -1.848714921503202 1101 26-27 -0.5516825751495986 1101 28-29 0.8091065650695768 1101 30-31 0.7911622465362456 1101 32-33 0.10950083534515898 1101 34-35 0.00464686264815839 1101 36-37 0.3509805155105141 1101 38-39 0.37383749380210674 1101 40-41 1.0349025919305248 1101 42-43 0.4192559865952674 1101 44-45 0.552528544879145 1101 46-47 0.5210572078077931 1101 48-49 -2.377294826577028 1101 50-51 -1.3243714211396878 1101 52-53 -1.547408441772511 1101 54-55 -0.580634726604373 1101 56-57 -0.8072062997173646 1101 58-59 -1.253639424291805 1101 60-61 -1.1786380938062209 1101 62-63 -0.5610570760219389 1101 64-65 -1.203372194256751 1101 66-67 -2.054532155787875 1101 68-69 -0.7599347002845107 1101 70-71 -1.1475706618911659 1101 72-73 -2.0999711718573266 1101 74-75 -0.800144183393968 1101 76 -1.4001060440809923 1102 1 -0.23379612830078855 1102 2 0.5665721700870492 1102 3 0.38007545258825814 1102 4 0.4788830483489903 1102 5 0.31906844602295337 1102 6 0.4349957391305068 1102 7 0.411365583778327 1102 8 0.49305355904373727 1102 9 0.23472171300256406 1102 10-11 0.26178495473956076 1102 12-13 0.4097691882428265 1102 14-15 0.015232494739578328 1102 16-17 0.20722025178491776 1102 18-19 -0.5975325492174619 1102 20-21 0.6249085440346605 1102 22-23 0.48669593658875954 1102 24-25 -4.791130081023624 1102 26-27 -1.1858524593194915 1102 28-29 0.2376271909586265 1102 30-31 1.6421071764285458 1102 32-33 0.349924529716219 1102 34-35 0.7687700083502591 1102 36-37 -2.4798719534998455 1102 38-39 -0.41695706015033807 1102 40-41 1.251220413511497 1102 42-43 0.7034461918381041 1102 44-45 1.095355206126861 1102 46-47 1.2780171752940817 1102 48-49 -2.5322430078409965 1102 50-51 -0.22390911673001312 1102 52-53 -0.07197153870402673 1102 54-55 0.6483583642308233 1102 56-57 0.8406295060131868 1102 58-59 -0.10158699592358289 1102 60-61 -5.189814683930177 1102 62-63 -2.421908528978655 1102 64-65 0.13377980736893136 1102 66-67 1.146951282537671 1102 68-69 -0.16638156067873666 1102 70-71 -0.8153465204565009 1102 72-73 -5.070581820099555 1102 74-75 -2.0834707430363153 1102 76 -0.8101344935831278 1103 1 0.7902188505334635 1103 2 0.40286328047454845 1103 3 -0.17536251550031778 1103 4 0.4409475224616557 1103 5 0.28080729330396537 1103 6 0.17115659878534473 1103 7 0.6522480324920963 1103 8 0.6424348680635958 1103 9 0.6779812375873888 1103 10-11 0.5539129260844007 1103 12-13 -0.5637329283315751 1103 14-15 -0.012038752411193343 1103 16-17 0.2679903592417716 1103 18-19 0.3365687207271719 1103 20-21 0.09698604322059623 1103 22-23 0.528457577747993 1103 24-25 -3.045403933189043 1103 26-27 -0.3214760347021013 1103 28-29 0.0472169011507404 1103 30-31 1.0536180849274075 1103 32-33 1.050958394906715 1103 34-35 0.2540353942180502 1103 36-37 -0.9214626405724644 1103 38-39 0.0751464891821243 1103 40-41 0.15980068052549967 1103 42-43 -0.7889829843260188 1103 44-45 1.017856020193939 1103 46-47 0.7286847102989711 1103 48-49 -2.0908558375381645 1103 50-51 -0.3532236722493849 1103 52-53 0.00923165243892754 1103 54-55 0.2209324443350269 1103 56-57 0.0821632083902557 1103 58-59 -0.01383056479365763 1103 60-61 -3.7336928994951393 1103 62-63 -1.1260765524237826 1103 64-65 0.09889703302832942 1103 66-67 0.305124516012107 1103 68-69 0.5795888072795847 1103 70-71 0.7341894613083966 1103 72-73 -4.620036395156539 1103 74-75 -1.9865967606201593 1103 76 -3.0212220872008437 1104 1 0.6728605283558622 1104 2 0.525948212796429 1104 3 0.46140678391958545 1104 4 0.06730480343741618 1104 5 -0.533784406829902 1104 6 -0.022878802077364924 1104 7 0.2254296478423896 1104 8 0.7842614335849447 1104 9 0.7484854767663265 1104 10-11 -0.5534052354506329 1104 12-13 0.3701879403282433 1104 14-15 1.0476816105220195 1104 16-17 0.5176556872203548 1104 18-19 0.8039939773090623 1104 20-21 -0.08938908692963565 1104 22-23 0.37800391123006705 1104 24-25 0.7527888378952952 1104 26-27 1.2424091356087743 1104 28-29 1.2316211849526155 1104 30-31 0.9217201227081588 1104 32-33 0.7872368420285341 1104 34-35 0.19769893727918486 1104 36-37 0.8520015867070256 1104 38-39 1.6213312281379544 1104 40-41 1.744547073504826 1104 42-43 0.7207968758554486 1104 44-45 0.868294812399796 1104 46-47 0.7613338252773971 1104 48-49 0.2265991676678425 1104 50-51 0.5341405996530426 1104 52-53 0.7324578907253994 1104 54-55 0.7250600659325173 1104 56-57 0.4045267365770826 1104 58-59 -0.18116657550316262 1104 60-61 0.9086170811682521 1104 62-63 0.6489122918421657 1104 64-65 0.9417127403018597 1104 66-67 0.6982525838389648 1104 68-69 1.719295783331944 1104 70-71 2.138398891622252 1104 72-73 1.630744506226769 1104 74-75 0.3623917861595487 1104 76 0.8419342418189402 1105 1 0.8844073693144736 1105 2 0.4796882273207572 1105 3 0.3825396815230704 1105 4 0.13230117235535488 1105 5 0.08059467813742316 1105 6 -0.008644960392544476 1105 7 0.6589808460994675 1105 8 -0.03389397672732741 1105 9 0.599030139075694 1105 10-11 0.588533762370723 1105 12-13 -0.2919398737603558 1105 14-15 0.28987478408774336 1105 16-17 0.1212504584621854 1105 18-19 0.5440811232785592 1105 20-21 0.595178814304937 1105 22-23 0.26006636584154563 1105 24-25 0.5351417790717647 1105 26-27 0.40588045006047935 1105 28-29 1.0097257601160194 1105 30-31 1.3616620253951623 1105 32-33 1.2052106982376785 1105 34-35 -0.215808687993146 1105 36-37 0.7326479193141466 1105 38-39 0.39289985558892937 1105 40-41 0.8349247060393239 1105 42-43 0.30757973714811726 1105 44-45 1.3456731711507075 1105 46-47 0.9328661419077591 1105 48-49 0.044863510441992105 1105 50-51 0.7246997862906568 1105 52-53 0.752392531248276 1105 54-55 0.8199402402244615 1105 56-57 0.8396756109416401 1105 58-59 0.6662190454118715 1105 60-61 1.2865764130491542 1105 62-63 0.26481643127571886 1105 64-65 0.8133903002147136 1105 66-67 0.43932012196533066 1105 68-69 0.5082209830414541 1105 70-71 1.0842957688916712 1105 72-73 1.0645135694076018 1105 74-75 -0.025044669904360717 1105 76 0.6503583522038383 1106 1 -0.3785378087567537 1106 2 0.1917330452479291 1106 3 0.27697745663311935 1106 4 -0.07958358154620981 1106 5 0.10680516233109927 1106 6 -0.029933475798706866 1106 7 -1.2197706545007705 1106 8 0.22328889642192706 1106 9 -0.6638350069827297 1106 10-11 0.04485629138232383 1106 12-13 0.83611134672784 1106 14-15 -0.8966998457641964 1106 16-17 -0.13427175042136952 1106 18-19 0.17115195160988605 1106 20-21 -0.14053547140935052 1106 22-23 0.2680895751252521 1106 24-25 -0.43768735258089464 1106 26-27 0.8462766085238655 1106 28-29 0.3569046316646336 1106 30-31 0.7823903167116839 1106 32-33 0.32181734089474645 1106 34-35 0.7337911519428246 1106 36-37 1.0729640457647207 1106 38-39 0.037997894804611576 1106 40-41 0.38488469003291925 1106 42-43 0.5572796171001002 1106 44-45 0.9118196297341328 1106 46-47 0.8366676625159997 1106 48-49 0.8115701931150596 1106 50-51 -0.3093338271547239 1106 52-53 0.6020724031970559 1106 54-55 -0.1647736453297597 1106 56-57 0.05960358213011574 1106 58-59 0.6663390934310769 1106 60-61 0.8743515230931749 1106 62-63 0.8499704929003684 1106 64-65 -0.7978942135908014 1106 66-67 0.34092076222142964 1106 68-69 1.2998576377033189 1106 70-71 0.3501020914206876 1106 72-73 0.10428947977174374 1106 74-75 -0.6418914085998395 1106 76 -0.5640874261074913 1107 1 0.3785967523847731 1107 2 -0.23451550295184376 1107 3 0.29767597994488426 1107 4 0.0612825209923642 1107 5 0.21079553896955616 1107 6 0.40154155323730123 1107 7 0.6518072449516907 1107 8 -0.4186142062825695 1107 9 -0.421342888183986 1107 10-11 0.513103346301854 1107 12-13 -0.12228420661544703 1107 14-15 0.374164597574115 1107 16-17 0.7053250639141169 1107 18-19 0.741211682819447 1107 20-21 0.7092032045488352 1107 22-23 -0.03975429412976439 1107 24-25 0.21925225252944358 1107 26-27 -0.3600449444875835 1107 28-29 0.8379897486382504 1107 30-31 0.319266042611801 1107 32-33 0.5518032376924893 1107 34-35 0.5265155587787334 1107 36-37 1.1373824960716732 1107 38-39 0.4516157809834809 1107 40-41 0.447083959984802 1107 42-43 0.5954204832026377 1107 44-45 0.8067922529297604 1107 46-47 0.9216394561114853 1107 48-49 0.94550913454529 1107 50-51 0.7329135596048673 1107 52-53 0.5356421725682168 1107 54-55 0.7214466964654989 1107 56-57 0.8404647070678948 1107 58-59 0.7266925030876266 1107 60-61 1.1368992251008052 1107 62-63 0.6941923279758555 1107 64-65 0.7880316201573336 1107 66-67 0.2249011836583037 1107 68-69 1.3787733503298298 1107 70-71 0.08626850920730789 1107 72-73 0.6135810012583889 1107 74-75 -1.3370963198326251 1107 76 0.17338561188820023 1108 1 -0.3505191012737612 1108 2 0.31167352143840077 1108 3 -0.1582691420063398 1108 4 0.30472764294358967 1108 5 0.31552114872565795 1108 6 0.1483403337251019 1108 7 0.2446426108053501 1108 8 -0.35779103555086067 1108 9 -0.058690449159598757 1108 10-11 0.5127984682530737 1108 12-13 1.106221890945534 1108 14-15 0.781786548793626 1108 16-17 -0.4090804238907566 1108 18-19 0.03709582916090426 1108 20-21 1.017053814304937 1108 22-23 -0.15004392827610502 1108 24-25 0.6309138378952923 1108 26-27 -0.5869504322924612 1108 28-29 -0.9305315928251687 1108 30-31 -0.22638944519307813 1108 32-33 0.6379312864729769 1108 34-35 0.6169119002421439 1108 36-37 0.9782361546082612 1108 38-39 1.1702895614712858 1108 40-41 0.89319676486285 1108 42-43 -0.06006732167541173 1108 44-45 0.5813349358565816 1108 46-47 0.6251455536724606 1108 48-49 0.8240914516184645 1108 50-51 0.6803983157024192 1108 52-53 -0.46268099816348496 1108 54-55 -0.29623623036377467 1108 56-57 0.16394031682399657 1108 58-59 0.4382778689412774 1108 60-61 0.9090028836373918 1108 62-63 0.9243752548051276 1108 64-65 0.49611088845001206 1108 66-67 0.5178127690241539 1108 68-69 0.8839562771591005 1108 70-71 -1.3081674664024447 1108 72-73 -1.3547879011806359 1108 74-75 -1.7300079051984767 1108 76 0.03969658749795002 1109 1 -0.8669487419923243 1109 2 0.31463010826475113 1109 3 0.4336844508080304 1109 4 0.2501243495304166 1109 5 0.24033402297715156 1109 6 -0.08268511537669099 1109 7 -0.2307690658413577 1109 8 -0.013217682257447905 1109 9 0.502200269403275 1109 10-11 0.5395200251393035 1109 12-13 0.5877712921431311 1109 14-15 0.8327596026858402 1109 16-17 0.8257062527559498 1109 18-19 0.6902769668854631 1109 20-21 0.8457776167001398 1109 22-23 0.0709328681310808 1109 24-25 0.16895650256594763 1109 26-27 0.2708152862704196 1109 28-29 -1.248252401208397 1109 30-31 0.18216220151350626 1109 32-33 0.5146154182095017 1109 34-35 -0.5204758243087468 1109 36-37 0.9268888492190399 1109 38-39 0.7136465973994248 1109 40-41 0.3001765552820146 1109 42-43 0.9623877681449429 1109 44-45 0.4433484089104809 1109 46-47 0.4348386674449145 1109 48-49 0.5050607629957113 1109 50-51 0.22873289654074824 1109 52-53 -0.1444549502592949 1109 54-55 -1.4439907213817378 1109 56-57 -0.3515536951520559 1109 58-59 -0.007680214891053083 1109 60-61 0.2849385123799024 1109 62-63 0.0669650751644113 1109 64-65 0.7156093914440262 1109 66-67 -0.02225085373034119 1109 68-69 -1.3532443216432952 1109 70-71 -0.5524601310730972 1109 72-73 -1.0640880508812316 1109 74-75 -0.5734772165757249 1109 76 0.24849149767759116 1110 1 -1.1960329049547447 1110 2 -0.08667770555545928 1110 3 0.044683311981394525 1110 4 -0.06455916687236396 1110 5 0.34385703829620695 1110 6 -0.1590599730233606 1110 7 0.4521962917869473 1110 8 0.4552074307068068 1110 9 0.8317620048281356 1110 10-11 -0.4877383415628742 1110 12-13 0.749741829595834 1110 14-15 1.087691456769086 1110 16-17 0.6120660485018732 1110 18-19 0.0837599395903581 1110 20-21 0.9212524339368358 1110 22-23 -0.5867578853313162 1110 24-25 -0.6459841682396714 1110 26-27 0.9930150585050868 1110 28-29 0.5658065973588862 1110 30-31 0.2873567204511005 1110 32-33 0.8957917159208364 1110 34-35 0.44440423153049124 1110 36-37 0.673481553381265 1110 38-39 0.7169345001215888 1110 40-41 -0.9986552290021748 1110 42-43 0.11017807709759353 1110 44-45 0.651772052421002 1110 46-47 0.3097697868012972 1110 48-49 1.2308974332135563 1110 50-51 -0.20942146957365537 1110 52-53 0.260746915946946 1110 54-55 -0.6279846966214393 1110 56-57 0.6483728321614137 1110 58-59 -0.53580188565995 1110 60-61 -0.05169496912334637 1110 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46-47 -0.2806673833904725 2116 48-49 0.42371994812195624 2116 50-51 -0.6979452157661115 2116 52-53 0.06035221861972673 2116 54-55 0.10184069271314655 2116 56-57 -0.08282541744173955 2116 58-59 -2.4937151380517264 2116 60-61 -1.6012680953835883 2116 62-63 0.15383329676316748 2116 64-65 -1.1444048458157212 2116 66-67 0.13693427252066215 2116 68-69 -0.8713758906730646 2116 70-71 1.188882358772382 2116 72-73 0.8736867491690177 2116 74-75 0.731639822074257 2116 76 -1.1049275383761739 2117 1 0.2237864542817931 2117 2 0.3415346325495179 2117 3 0.0632586357714402 2117 4 -0.0654112459452989 2117 5 -0.1657288512743449 2117 6 -0.032909666274896665 2117 7 0.36894816636090866 2117 8 -0.011263257773080682 2117 9 -0.8302182269373759 2117 10-11 -0.12053486508025202 2117 12-13 -1.2972503312766932 2117 14-15 -0.23974122898415118 2117 16-17 -1.0413720905574237 2117 18-19 -0.7365152819502043 2117 20-21 -0.20204340791728725 2117 22-23 0.29196996061278213 2117 24-25 -1.334016717660262 2117 26-27 -1.9539643211813456 2117 28-29 -1.1919899261584987 2117 30-31 -0.05590333408196102 2117 32-33 -0.9627631579714659 2117 34-35 0.4766341224643682 2117 36-37 -0.8300971787250688 2117 38-39 0.2602201170268401 2117 40-41 -0.7099282351371485 2117 42-43 0.18993267832458827 2117 44-45 -0.4526928419211842 2117 46-47 -0.7186044463275394 2117 48-49 -0.8123668817148726 2117 50-51 -0.9859211287420209 2117 52-53 0.16995789072539935 2117 54-55 -0.5260973414748875 2117 56-57 -0.004809683176006274 2117 58-59 -0.9554721310587198 2117 60-61 -0.16044156080705108 2117 62-63 -0.3395136340837581 2117 64-65 -0.8858335559944308 2117 66-67 -0.5367011198647305 2117 68-69 -0.005627056174226652 2117 70-71 -0.372403577513559 2117 72-73 0.7441704321526927 2117 74-75 0.8238115392459662 2117 76 1.4098354763868386 2118 1 -1.1178429267771186 2118 2 0.03705103821693001 2118 3 -1.0081433030801605 2118 4 0.3385783140845291 2118 5 0.45465705476592433 2118 6 -0.22040966627489667 2118 7 0.7655738188590462 2118 8 0.6451032597511528 2118 9 -1.033145147146172 2118 10-11 -0.6919834109415532 2118 12-13 -1.47058180032964 2118 14-15 0.6263335286594014 2118 16-17 -2.848488980937738 2118 18-19 -0.6142883990270107 2118 20-21 -0.8279755481111764 2118 22-23 0.6956565750796031 2118 24-25 0.4943995761503288 2118 26-27 -2.610167714171652 2118 28-29 -0.3968277337647592 2118 30-31 -0.274816458615895 2118 32-33 0.4414128300971356 2118 34-35 0.3032793499065747 2118 36-37 -2.4078376708950913 2118 38-39 -0.7752012103408035 2118 40-41 -0.8303141411774178 2118 42-43 0.08255012798901618 2118 44-45 -2.6598143930024705 2118 46-47 -0.24628901008592408 2118 48-49 0.39030369994061687 2118 50-51 -0.7436578923512656 2118 52-53 -1.6262296558816018 2118 54-55 -0.6333167672765256 2118 56-57 -0.019700622773321186 2118 58-59 -0.8860509901191236 2118 60-61 -1.0417102036109256 2118 62-63 -1.190138168013661 2118 64-65 0.03281390858423805 2118 66-67 -0.04441457996913556 2118 68-69 -1.8981745953241216 2118 70-71 -0.06917837244271396 2118 72-73 0.9313907900945324 2118 74-75 0.06812548406327323 2118 76 0.5981277955516475 2119 1 0.653525016373294 2119 2 -0.43501765503218337 2119 3 0.10901026975836459 2119 4 -0.14711059235052915 2119 5 0.17618291343153913 2119 6 -0.8159979015690126 2119 7 -0.0836662127240615 2119 8 0.11169425856678572 2119 9 -0.22155809621842337 2119 10-11 0.006916115311902615 2119 12-13 0.15658953800435427 2119 14-15 -0.05938992179460456 2119 16-17 -0.08242601212605649 2119 18-19 -0.18606593554497408 2119 20-21 -0.9026153033421238 2119 22-23 -0.3561101047466977 2119 24-25 0.8667594261305922 2119 26-27 0.47720397947225024 2119 28-29 -0.042480122236931095 2119 30-31 1.1822502606892726 2119 32-33 0.02212246294357101 2119 34-35 0.34816190024214677 2119 36-37 0.41132438990237574 2119 38-39 -0.49386485029342 2119 40-41 -0.7356635292547935 2119 42-43 0.27816797244224034 2119 44-45 0.016261406444819215 2119 46-47 0.15271908308422866 2119 48-49 -0.19410707779330494 2119 50-51 0.616611550996538 2119 52-53 -0.1784898216928994 2119 54-55 0.08685200493034273 2119 56-57 -1.5213538008230643 2119 58-59 -0.14113389576460378 2119 60-61 0.5983411189315078 2119 62-63 -0.5903306275478144 2119 64-65 -0.21160969978527788 2119 66-67 0.1628495337300393 2119 68-69 0.928809218335573 2119 70-71 0.2497369453622582 2119 72-73 1.5431900399958351 2119 74-75 0.3646612124485813 2119 76 0.4547701169097209 >>END_MODULE >>Per sequence quality scores pass #Quality Count 20 1.0 21 2.0 22 4.0 23 6.0 24 25.0 25 49.0 26 105.0 27 178.0 28 313.0 29 545.0 30 863.0 31 1400.0 32 2044.0 33 3277.0 34 5165.0 35 7825.0 36 13563.0 37 22512.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 35.96938334744372 23.765917376505346 11.833716329457298 28.430982946593637 2 17.215819755688788 19.30300464778755 39.539022409592754 23.942153186930906 3 16.861620332774677 25.472985814745066 31.190282841197714 26.47511101128255 4 12.504103529899616 20.38979214541182 39.16754496604869 27.938559358639875 5 12.05141938939475 37.5710558598407 37.479482350501925 12.898042400262627 6 26.229763118337164 41.09749987041484 18.28360143062011 14.389135580627881 7 25.448796585863125 30.79288836670871 24.170223059246332 19.588091988181834 8 22.24199595694317 37.27905731119443 20.94441660763343 19.53453012422897 9 22.825993054235706 15.071617395511169 22.92793337595245 39.17445617430067 10-11 24.00780966532474 26.183976363667778 27.808110302883705 22.00010366812378 12-13 23.54994211863089 23.00395666672426 27.336420339685887 26.10968087495896 14-15 20.9167717746255 25.028940684555177 24.085560758159545 29.968726782659775 16-17 23.086027264716556 27.99471292568723 25.221590614579192 23.697669195017017 18-19 22.629023619054202 27.091936347772 26.320472726644432 23.958567306529364 20-21 21.566425350311867 27.20078787774073 25.959362095478344 25.273424676469062 22-23 21.506816179138518 27.810702005978193 27.24743853344161 23.43504328144168 24-25 22.337025070407933 28.411977123900684 26.175337353352802 23.07566045233858 26-27 22.45451561069164 28.196865767057727 26.982220916771777 22.36639770547886 28-29 22.17547557751784 26.71009209184996 28.289303177427993 22.82512915320421 30-31 21.719335832886983 26.977037510582786 28.714342484924927 22.5892841716053 32-33 21.28652141610657 29.57219620920227 26.670352644401056 22.4709297302901 34-35 21.56556144928037 28.500095029113464 27.369248578882804 22.565094942723363 36-37 22.651485045873144 27.4504552758436 26.86904988164556 23.0290097966377 38-39 21.774625498902846 27.007274046685207 27.903139416348466 23.31496103806348 40-41 21.091279782988064 25.647493823107624 29.319937108004908 23.94128928589941 42-43 22.53399450558944 25.634535307635158 28.873300274720528 22.958169912054874 44-45 20.47359054546711 26.78438758055877 28.994246419130228 23.747775454843893 46-47 22.052801631045146 25.376228899217306 28.5674793095703 24.003490160167253 48-49 21.122380220121983 25.277744181626556 29.516042642154915 24.083832956096547 50-51 19.437773208701213 26.154603728596854 29.874561570226515 24.533061492475422 52-53 19.543169134543948 25.945539678974377 31.23866129896159 23.272629887520086 54-55 18.428736803911743 26.484613922629023 30.428322131416625 24.65832714204261 56-57 19.858493011040657 26.273822070943552 29.0054771325397 24.862207785476095 58-59 19.02396461461375 26.79389049190525 29.90393420529744 24.278210688183563 60-61 20.302538141230542 27.481555712977524 27.800335193600223 24.415570952191718 62-63 18.471067954455137 29.11864816766591 27.16795963854381 25.24232423933514 64-65 19.048153843495687 29.76743784232078 25.87297199232856 25.31143632185497 66-67 19.130224441487982 29.67240872885602 25.67773035921005 25.519636470445946 68-69 19.201928227102304 29.45816127304456 25.54296179829639 25.796948701556747 70-71 19.86108471413515 28.954506971681326 24.984017830917292 26.20039048326624 72-73 19.552672045890425 28.427527342467645 25.361542581681846 26.658258029960084 74-75 19.942291411095944 28.517373049743423 25.33044214454792 26.209893394612717 76 19.966480639977885 28.164901428892307 25.89975292430499 25.96886500682482 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.5 10 0.5 11 0.0 12 0.0 13 0.0 14 0.5 15 2.5 16 5.0 17 6.0 18 5.5 19 5.0 20 6.5 21 8.0 22 8.5 23 20.0 24 40.5 25 50.0 26 103.5 27 173.5 28 264.5 29 339.0 30 358.5 31 452.5 32 656.5 33 786.0 34 968.5 35 1270.5 36 1560.5 37 1731.0 38 2011.5 39 2723.5 40 3624.0 41 4133.5 42 4174.0 43 4360.5 44 4439.5 45 4397.0 46 4462.0 47 4524.5 48 4353.0 49 3817.0 50 3515.0 51 3223.5 52 2601.5 53 2069.5 54 1868.0 55 1704.5 56 1275.0 57 900.5 58 792.0 59 665.5 60 426.5 61 292.5 62 271.0 63 200.0 64 103.0 65 66.5 66 38.5 67 21.0 68 16.0 69 6.0 70 0.5 71 0.0 72 0.0 73 0.0 74 0.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 57877.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 40.0780966532474 #Duplication Level Percentage of deduplicated Percentage of total 1 57.93671322641835 23.21993192459872 2 15.011208829108465 12.032413566701798 3 9.230039662010691 11.097672650621144 4 5.406104500775996 8.666655147986246 5 3.3195378513536813 6.652037942533304 6 2.2934988791170894 5.515144185082157 7 1.599413692015865 4.487101957599737 8 1.1208829108466978 3.5938282910309796 9 0.8579065356095879 3.0944934948252327 >10 3.19882738403173 18.855503913471672 >50 0.00862217623728229 0.2021528413704926 >100 0.012933264355923435 1.1610829863330856 >500 0.004311088118641145 1.4219810978454308 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGT 823 1.4219810978454308 No Hit ATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGTCTT 427 0.7377714808991482 Illumina PCR Primer Index 9 (95% over 22bp) ATAAAGAATATTGAGGCGCCATTGGCGTGAAGGTAGCGGATGATTCAGCC 139 0.24016448675639718 No Hit CTCTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCC 106 0.1831470186775403 No Hit TCTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCG 59 0.10194032171674412 No Hit CTTTACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGTC 58 0.10021251965374846 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0034556041259913265 0.0 2 0.0 0.0 0.0 0.00518340618898699 0.0 3 0.0 0.0 0.0 0.00518340618898699 0.0 4 0.0 0.0 0.0 0.012094614440969643 0.0 5 0.0 0.0 0.0 0.015550218566960969 0.0 6 0.0 0.0 0.0 0.015550218566960969 0.0 7 0.0 0.0 0.0 0.02246142681894362 0.0 8 0.0 0.0 0.0 0.031100437133921938 0.0 9 0.0 0.0 0.0 0.039739447448900254 0.0 10 0.0 0.0 0.0 0.051834061889869894 0.0 11 0.0 0.0 0.0 0.06047307220484821 0.0 12 0.0 0.0 0.0 0.062200874267843875 0.0 13 0.0 0.0 0.0 0.06738428045683087 0.0 14 0.0 0.0 0.0 0.07429548870881351 0.0 15 0.0 0.0 0.0 0.07947889489780051 0.0 16 0.0 0.0 0.0 0.08293449902379184 0.0 17 0.0 0.0 0.0 0.08639010314978315 0.0 18 0.0 0.0 0.0 0.09848471759075281 0.0 19 0.0 0.0 0.0 0.10021251965374847 0.0 20 0.0 0.0 0.0 0.10712372790573112 0.0 21 0.0 0.0 0.0 0.11230713409471811 0.0 22 0.0 0.0 0.0 0.11230713409471811 0.0 23 0.0 0.0 0.0 0.11576273822070944 0.0 24 0.0 0.0 0.0 0.1174905402837051 0.0 25 0.0 0.0 0.0 0.1174905402837051 0.0 26 0.0 0.0 0.0 0.1313129567876704 0.0 27 0.0 0.0 0.0 0.1451353732916357 0.0 28 0.0 0.0 0.0 0.17969141455154897 0.0 29 0.0 0.0 0.0 0.2211586640634449 0.0 30 0.0 0.0 0.0 0.24707569500837984 0.0 31 0.0 0.0 0.0 0.3040931630872367 0.0 32 0.0 0.0 0.0 0.32137118371719336 0.0 33 0.0 0.0 0.0 0.3956666724260069 0.0 34 0.0 0.0 0.0 0.4509563384418681 0.0 35 0.0 0.0 0.0 0.5701746807885688 0.0 36 0.0 0.0 0.0 0.7809665324740398 0.0 37 0.0 0.0 0.0 1.1472605698291203 0.0 38 0.0 0.0 0.0 1.6120393247749538 0.0 39 0.0 0.0 0.0 2.237503671579384 0.0 40 0.0 0.0 0.0 2.9614527359745666 0.0 41 0.0 0.0 0.0 3.7061354251256975 0.0 42 0.0 0.0 0.0 4.466368332843789 0.0 43 0.0 0.0 0.0 5.373464415916513 0.0 44 0.0 0.0 0.0 6.081863261744735 0.0 45 0.0 0.0 0.0 6.988959344817458 0.0 46 0.0 0.0 0.0 7.833854553622337 0.0 47 0.0 0.0 0.0 8.666655147986246 0.0 48 0.0 0.0 0.0 9.44589387839729 0.0 49 0.0 0.0 0.0 10.073086027264717 0.0 50 0.0 0.0 0.0 10.78148487309294 0.0 51 0.0 0.0 0.0 11.507161739551117 0.0 52 0.0 0.0 0.0 12.251844428702247 0.0 53 0.0 0.0 0.0 12.941237451837518 0.0 54 0.0 0.0 0.0 13.67728113067367 0.0 55 0.0 0.0 0.0 14.363218549682948 0.0 56 0.0 0.0 0.0 14.959310261416451 0.0 57 0.0 0.0 0.0 15.588230212346874 0.0 58 0.0 0.0 0.0 16.21887796534029 0.0 59 0.0 0.0 0.0 16.80114726056983 0.0 60 0.0 0.0 0.0 17.260742609326677 0.0 61 0.0 0.0 0.0 17.824006081863264 0.0 62 0.0 0.0 0.0 18.463292845171658 0.0 63 0.0 0.0 0.0 19.0438343383382 0.0 64 0.0 0.0 0.0 19.686576705772588 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CACTCAG 15 0.00221421 70.00001 7 CTAGAAC 20 0.0069188783 52.5 24 GAGTGAA 20 0.0069188783 52.5 28 GACAATC 20 0.0069188783 52.5 57 ATCAAGC 20 0.0069188783 52.5 39 TACATAC 20 0.0069188783 52.5 3 AAGCCCG 20 0.0069188783 52.5 42 AGTGAAG 20 0.0069188783 52.5 29 TGACAAT 20 0.0069188783 52.5 56 AAACCTT 20 0.0069188783 52.5 19 CCGAATG 20 0.0069188783 52.5 46 CAATCGA 20 0.0069188783 52.5 59 AGGTGGG 20 0.0069188783 52.5 7 GCCCGAA 20 0.0069188783 52.5 44 ATCGAGT 20 0.0069188783 52.5 61 AATCGAG 20 0.0069188783 52.5 60 TATGCCG 225 0.0 40.444447 43 ATGCCGT 225 0.0 40.444447 44 TGAAAAA 210 0.0 40.000004 59 CAGAGAA 35 0.001245911 40.0 41 >>END_MODULE