FastQCFastQC Report
Wed 25 May 2016
SRR1780563_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780563_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences85
Sequences flagged as poor quality0
Sequence length50
%GC58

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACATTTTCTACCCCAGAAAACTACGATAGCCCTTATGAAACTCTGTCTCT11.1764705882352942No Hit
CTCTTATACACATCTGACGCTGCCGACGAATCTGGCGGTGTAGATCTCGG11.1764705882352942No Hit
GCACGGCGGTGCCCACGTTGAAGTTCCTGGGCGTGGTGTAGCTGGGGCCT11.1764705882352942No Hit
CTACACAAAGTGAGCGGCAGCAGAGATCCGTGCGTGAGAGAGAGAGAGAG11.1764705882352942No Hit
ATCCTAGCTATTCGGGAAGCTGAGGAGGGAGGATCGCTTGAGCCCAGGAG11.1764705882352942No Hit
CTAGACCCCTATCCCCGGCAACGCCCATTACCGACAACAGCTCCCCCCCA11.1764705882352942No Hit
ACATAAGCTTCTGACTCTTACCTCCCTCTCTCCTACTCCTGCTCGCATCT11.1764705882352942No Hit
CGCCAGGACCGCGTGATGACGCGCATGACCTGGGCGCTGTCTCTTATACA11.1764705882352942No Hit
CTCTTATCCAGTAAGCCTTGTCCATAGTAGCTGGCCATAGTCATATCAGC11.1764705882352942No Hit
GTATTTGTTAACCTTATTAAGTTGAGAACTTTCACCTTTTCACTTAAAGG11.1764705882352942No Hit
GATATGGGGAGTAGTGTGATTGAGGTGGAGTAGATTAGGCGTAGGTAGAA11.1764705882352942No Hit
CCCCCGCAGTGCCCGGCATCGTGGCCGTGGTGGCCGAGGAGCTGTCTCTT11.1764705882352942No Hit
CACCTACACCCCTTATCCCCATACTAGTTATTATCGAAACCATCAGCCTA11.1764705882352942No Hit
GGGAGACGGCCGTCTGCCGGCCCTGGGGGTCCGGGGCGACCATCGGCCCT11.1764705882352942No Hit
TCCTCCGGCTCGACGGCGACCGGGCCGCGATCCGGGCTGTCTCTTATACA11.1764705882352942No Hit
GGCCAGGAGCAGCTCGACGCCGGCCGCGCCGAGCTCGAGGAGCAGGCGCT11.1764705882352942No Hit
GCCTACCCCCGCTTCCGCTCCGACCTCCTCATCCCGCTCCACCGAAGCAA11.1764705882352942No Hit
GGCGGCGACAAGGTGCGAGGAAGGGCCGCCAACCGGAGCTTGTCTCTTAT11.1764705882352942No Hit
CTTTACCTCTGACGCCGCCGTCGAGTACGGCCGGGGGGTACTCGGCGCGC11.1764705882352942No Hit
CGGCCAGTGTGAAGAAGCCAGCGTCGTATGACGAGACGGTTTGGGTCGGT11.1764705882352942No Hit
TGCCTAGGCCGATAAGTTATTATCTCTAACGGACACTGAGCCACAAGCCA11.1764705882352942No Hit
GTGCCAGCCACCGCGGTCACACGATTAACCCAAGTCAATAGACTGTCTCT11.1764705882352942No Hit
CGCCTGAACCGCGGCCGGTCGGCCTGAGCGCCCCTGGGCCTGTCTCTTAT11.1764705882352942No Hit
ACCAAAGCCCATAAAAATAAACACTTATAACATACCCGGAGAACCAAAAC11.1764705882352942No Hit
ATTGAAAGCAGGAAATCAAAGTGGCAAAGGTCATATGACTCTTGGGGTTC11.1764705882352942No Hit
CTCACGCACGTGCGCGGAGGCGCCGGTGATCTCGGCGCCCCGTCCGGCGC11.1764705882352942No Hit
GGGCCACCCCGTGCGCGCCTACCTCGACGTGGACGAGATCATCCGCGTGG11.1764705882352942No Hit
CTTATACACATCTGACGCTGCCGACGAGAATGATCGTGTAGATCTCGGTG11.1764705882352942No Hit
ACCCAAGCGAACACGTCCAACCGCTTCCCCTCCCTCGTAGAACTCTCCCA11.1764705882352942No Hit
CTCCCTAGGAGGCCTGCCCCCGCTAACCGGCTTTTTGCCCAAATGGGCCA11.1764705882352942No Hit
CTTTTCACATCGGACGCGGCCGACGAGGGGGTGCGGGTCGGGTTTGTCGG11.1764705882352942No Hit
CCTACTATGGGTGTTAAATTTTTTACTCTCTCTGCAAGGTTTTTTCCTAG11.1764705882352942No Hit
CCCGTCCGGCTGGCCCGGGAGCTGCGCGCGGCCGGAGCGAAGGCCGGCAT11.1764705882352942No Hit
CCCACTAGGATACCAACAAACCTACCCATCTTTAACAGTACATAGTACAT11.1764705882352942No Hit
GGGCTAAGCATAGTGGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGT11.1764705882352942No Hit
CGTCATGGTGCCGCCGGTGAGCGCCTTGCCGAGGCACAGCACGTCCGCTG11.1764705882352942No Hit
CTTCCACGGACTTCACGTCATTATTGGCTCAACTTTCCTCACTATCTGCT11.1764705882352942No Hit
GTTACTACGAGGGCTATGTGGCTGATTGAAGAGAATGCAATGAACGATCT11.1764705882352942No Hit
TCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCAC11.1764705882352942No Hit
GGTTTATAGATAGTTGGGTGGTTGGTGTAAATGAGTGAGGCAGGAGTCCG11.1764705882352942No Hit
CTCTTATACACATCTGACGCTGCCGACGATTGGTACGGTGTAGATCTCGG11.1764705882352942No Hit
GCCTGCTGCTGCACGGCCTGCGGGGCCTGTCTCTTATACACATCTGACGC11.1764705882352942No Hit
CCGCGGCCATCCGCCTCGACCCGGACTTCCAGGGCTGTCTCTTATACACA11.1764705882352942No Hit
CCGTCGAAGCGGACCCGGCCGGGCCGCAGCGCGGCGGCTGTCTCTTATAC11.1764705882352942No Hit
GTTATACACATCTGACGCGGCCGCCCAATGACCATGTCCGCAACTCAGGC11.1764705882352942No Hit
CACCTGCTCTCCGTGCTCGTCCACGCGCGCCGCGGACGGACGTGGCCCTG11.1764705882352942No Hit
CCCCTTCGCCCTATTCTTCATAGCCGAATACACAAACATTATTATAATAA11.1764705882352942No Hit
GCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCT11.1764705882352942No Hit
GCCCACGATGTAGCCGAACGTGTCGTTGGAGACCACCATCAGCCTGTCTC11.1764705882352942No Hit
GGGATCCCGGCGGGGGGGACCGAGGGAGTAGGGGAGGGTGCGAACGGGGG11.1764705882352942No Hit
GTCCTGGCCGCCACGTGAGGGGGATTCGGCTCCCCCCTCGCCGCGTGCTT11.1764705882352942No Hit
GGAGGCTGGCAAGTCGGGTTCTTCATCTCTCCAGGCATGTTTCCCTACTT11.1764705882352942No Hit
ACCCAGCCCATGACCCCTAACAGGGGCCCTCTCAGCCCTCCTAATGACTG11.1764705882352942No Hit
ATCTGACGATGCCGACGACCCGACCCGGTACTATACGACACGTACCACGT11.1764705882352942No Hit
ATTCCTAGCCATACACTACTCACCAGACGCCTCAACCGCCTTTTCATCAA11.1764705882352942No Hit
GGGCGAGCTCGCGGCGGGCCTCCACCCCACCCCCGAGTGTCCCTTTTACA11.1764705882352942No Hit
GCGCGGTCTCGAGCGCGAGGTCGAGCAGGGCGTCCGCCGCGGGGGAGTGG11.1764705882352942No Hit
CTGTCTCTTATACACATCTGACGCTGCCGACGATCCGTGCCGTGTAGATC11.1764705882352942No Hit
AACTATCACAGAGACAAAAAACCAAACACTGGATATTCTCACTCATAGGT11.1764705882352942No Hit
ACCTGATGGAGCCCGGCGGTGTCGCGCGGAGCCGGCTCTGTCTCTTATAC11.1764705882352942No Hit
GCCGAGGGCCGTCACGGAGGTCGGGGTCTGCTTGGCCGTGCAACCCCGCC11.1764705882352942No Hit
AACTAACCTCAAAACAAATAATAACCATACACAACACTAAAGGACGAACC11.1764705882352942No Hit
TAGTAAGATTACACATGCAAGCATCCCCATTCCAGTGAGTTCACCCTCTA11.1764705882352942No Hit
TCCGCGGAGCGTCCCCAGCGACTGGTTCCTCGACCCCGCCGACCCGCAGG11.1764705882352942No Hit
GACGTCGCCTCCCCCGCCCTGACCGGCGGCATCCAGGGCCTGTCTCTTAT11.1764705882352942No Hit
CTTCTTACTCATGCGCTTCCACCCCCTAGCAGACAATAGCCCACTAATCC11.1764705882352942No Hit
CCCGGGGCGTGAGCGCGTGATCGGCGTGTACGACCTGTCTCTTATACACA11.1764705882352942No Hit
CCTCCGCTCTGTGGGCCAGCGCGGGGGCGCGGGGCGGGCAGGATCGATGC11.1764705882352942No Hit
GCCGGGGGTCCACCGCCGCGAGCACGGGCACGAGCGCCCGCACCGCGAGC11.1764705882352942No Hit
GGCCTGCTTCTTCTCACATGACAAAAACTAGCCACCCTCTCAATCATATA11.1764705882352942No Hit
CCTTACACCTAGCAGGTGTCTCCTCTATCTTAGGGGCCATCAATTTCATC11.1764705882352942No Hit
AATCACTTTATTGACTCCTAGCCGCAGACCTCCTCATTCCTGTCTCTTAT11.1764705882352942No Hit
CGCTGACACCGCGCGGGGCCCTCCTCCTGACCGGAGCGGTCAACACCTGT11.1764705882352942No Hit
AGTCAATAGACGCCGGCGTAAAGAGTGTTTTAGATCACCCCCTCCCCAAT11.1764705882352942No Hit
CCCTAAGTCTGGCCTATGAGTGACTACAAAAAGGATTAGACTCTGTCTCT11.1764705882352942No Hit
TCTCCACGGTGTTGAATCTCTGGAGCTGCCCATAGCCTGTCTCTTATACA11.1764705882352942No Hit
GGGAGTACGCGCAGCCGAGCGAGTGGTCCGAGGGCGAGGGCCTGGACTGG11.1764705882352942No Hit
CTCTTATACACATCTGACGCTGCCGACGCATGACCATGTGTTGAATTCGG11.1764705882352942No Hit
GTTACCCATTACTGCCAAGTTAAACTGGTTTTTTTTTTTTTTTAAGAAAA11.1764705882352942No Hit
ACAAACCCAACCGCCCGCGAGCGCCTGTACCCAACTAACGCCGCCTCCTC11.1764705882352942No Hit
GCGTACGCGTTTACAGATATGCCGCCGGCGTACTGATCCGACCAGGAGCC11.1764705882352942No Hit
CACCACGGCCGACGCGGCCCAGGTCCCCGCCCGCCCCGACGACGCGGCGG11.1764705882352942No Hit
GGGCGGGAGGTTGATCGTTCTTGGGCAGTGAGAGTGCGAAGTAGAATGTT11.1764705882352942No Hit
GCCGAGCGTCTTGAGGCCGCGGGCGGCGAGGTAGGACTGTCTCTTATACA11.1764705882352942No Hit
GTTGGAGGCCTGGGCGCCCATCATCACGTCTGTGTCGAGCGGGTTGCCGG11.1764705882352942No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGGGC50.044.020
TGCACGG50.044.010
CACATCT50.044.039
TATACAC50.044.035
CCTGCTG50.044.02
CCTGCGG50.044.017
TGTCTCT50.044.028
CGGGGCC50.044.021
CCTGTCT50.044.026
CATCTGA50.044.041
CTCTTAT50.044.031
TGCTGCT50.044.04
TGCTGCA50.044.07
CTGACGC50.044.044
ACATCTG50.044.040
TTATACA50.044.034
TACACAT50.044.037
ACACATC50.044.038
GCTGCTG50.044.05
CTGCGGG50.044.018
Produced by FastQC (version 0.11.5)