FastQCFastQC Report
Wed 25 May 2016
SRR1780563_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780563_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences85
Sequences flagged as poor quality0
Sequence length50
%GC59

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCGT33.5294117647058822No Hit
AGCCCACGAGACAAACGGTCATCTCGTATGCCGTCTTCTGCTTGAAAAAA11.1764705882352942Illumina PCR Primer Index 7 (96% over 28bp)
GACCCCGCGGTGCGCCTCATCCCACGAGACGGGCCAGTCCAGGCCCTCGC11.1764705882352942No Hit
CCTCTCCCTAGGAGGCCTGCCCCCGCTAACCGGCTTTTTGCCCAAATGGG11.1764705882352942No Hit
GCCTCCACTCCCCCGCGGCGGACGCCCTGCTCGACCTCGCGCTCGAGACC11.1764705882352942No Hit
TCTTATACACATCTCCGAGCCCACGAGCGGCGTCGAATCTCGTATGCCGT11.1764705882352942No Hit
AGTTTCATAAGGGCTATCGTAGTTTTCTGGGGTAGAAAATGTCTGTCTCT11.1764705882352942No Hit
CTCTGACATCCGGCCTGCTTCTTCTCACATGACAAAAACTAGCCCCCATC11.1764705882352942No Hit
GCACCAGCTACACCACGTCCATGAACTTCAACGTGGGCACCGCCGTGCCT11.1764705882352942No Hit
GAGCACTAGCTGATATGACTATGGCCAGCTACTATGGACAAGGCTTACTG11.1764705882352942No Hit
GGCCAGGGGCACGCCGGCCTCGGTGCAGCGGCGCGCCAGTTCGGCGACGG11.1764705882352942No Hit
GCCCTGGATGCCGCCGGTCAGGGCGGGGGAGGCGACGTCCTGTCTCTTAT11.1764705882352942No Hit
GTCCGAGCCCACGAGACAAACGGTCATCTCGTATGCCGTCTTCTGCTTGA11.1764705882352942Illumina PCR Primer Index 7 (96% over 28bp)
AGCCCGCACTGCCCTCGGGCATCGCCCTCGTGGACGCCCCGGACATCGAC11.1764705882352942No Hit
CTGCAGCCTTGACCTCCTGGGCTCAAGCGATCCTCCCTCCTCAGCTTCCC11.1764705882352942No Hit
ATCTCCGAGCCCACGAACGATCCAAAATCTCGTATGCCGTCTTCTGCTTG11.1764705882352942TruSeq Adapter, Index 6 (96% over 29bp)
GCGTAAAGAGTGTTTTAGATCACCCCCTCCCCAATAAAGCTAAAACTCAC11.1764705882352942No Hit
GAATGAGGAGGTCTGCGGCTAGGAGTCAATAAAGTGATTCTGTCTCTTAT11.1764705882352942No Hit
CCCACGAGACAGCCCACGAGACGGTAAACCATCTCGTATGCCGTCTTCTG11.1764705882352942Illumina PCR Primer Index 1 (95% over 23bp)
GCCGAGGCCCGCGCCGGACGGGGCGCCGAGATCACCGGCGCCTCCGCGCA11.1764705882352942No Hit
GCCCAGGGGCGCTCAGGCCGACCGGCCGCGGTTCAGGCGCTGTCTCTTAT11.1764705882352942No Hit
CGTCGAGCCCACGAGACCCAACACGATCTCGTATGCCGTCTTCTGCTTGA11.1764705882352942Illumina PCR Primer Index 1 (96% over 31bp)
GGTAGAAGTAGAGGTTAAGGAGGGTGATGGTGGCTATGATGGTGGGGATG11.1764705882352942No Hit
GCAGTAATGTTAGCGGTTAGGCGTACGGCCAGGGCTATTGGTTGAATGAG11.1764705882352942No Hit
CGGACGTGCTGTGCCTCGGCAAGGCGCTCACCGGCGGCACCATGACGCTG11.1764705882352942No Hit
ATGTTGTTCCTAGGAAGATTGTAGTGGTGAGGGTGTTTATTATAATAATG11.1764705882352942No Hit
GCCAGGTCCTTTGAGTTTTAAGCTGTGGCTCGTAGTGTTCTGGCGAGCAG11.1764705882352942No Hit
GCCCCGCAGGCCGTGCAGCAGCAGGCCTGTCTCTTATACACATCTCCGAG11.1764705882352942No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCC11.1764705882352942No Hit
GCAGCGTCAGATGTGTATAAGAGACAGCCATCTCCATCCCACGAGACATT11.1764705882352942No Hit
CCGCACAAGAGTGCTACTCTCCTCGCTCCGGGCCCATAACACTTGGGGGT11.1764705882352942No Hit
CATGAAAGGTGATAAGCTCTTCTATGATAGGGGAAGTAGCGTCTTGTAGA11.1764705882352942No Hit
CTCCTCGGCCACCACGGCCACGATGCCGGGCACTGCGGGGGCTGTCTCTT11.1764705882352942No Hit
ATCCATGGGGACGAGAAGGGATTTGACTGTAATGTGCTATGTACGGTAGA11.1764705882352942No Hit
CCGGGGAAGAGGGCGGTGACGGTGGCGGCCGGGGTGCCGGTCTCGCCGTC11.1764705882352942No Hit
AGCCGGCTCCGCGCGACACCGCCGGGCTCCATCAGGTCTGTCTCTTATAC11.1764705882352942No Hit
AACTCATCATTGACATTAGGTATATCTCCCGATGCTATCCCTCCACCCTC11.1764705882352942No Hit
GTCGTACACGCCGATCACGCGCTCACGCCCCGGGCTGTCTCTTATACACA11.1764705882352942No Hit
ATTTATACTAAAAGAGTAAGACCCTCATCAATAGATGGAGACATACAGAA11.1764705882352942No Hit
ACTTTGTTGTGTTTTTTTAAAACTTTACTTTCCACTGCCATGGTAACTGC11.1764705882352942No Hit
GGCCGATGGTCGCCCCGGACCCCCAGGGCCGGCAGACGGCCGTCTCCCCT11.1764705882352942No Hit
GACTCGCCGGGGTTCAAGCTCAAGCCGAACCCGGCCACCGTGACGGCCGA11.1764705882352942No Hit
TCTATTGACTTGGGTTAATCGTGTGACCGCGGTGGCTGGCACCTGTCTCT11.1764705882352942No Hit
CTGCCTGATCGTCATCCCCATCGGCCTGCTGACCTCGTGGGCTCGGAGAT11.1764705882352942No Hit
CCCACGAGACGGTAAACCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA11.1764705882352942Illumina PCR Primer Index 1 (96% over 27bp)
GCCCCGGCGTCCTTCGCCACGCGGATGATCTCGTCCACGTCGAGGTAGGC11.1764705882352942No Hit
CTTACCCTGGGGGAAGCAAGGGGCCTGGTCCCAAGTAGGGAAACATGCCT11.1764705882352942No Hit
TCGCCGCGCTGCGGCCCGGCCGGGTCCGCTTCGACGGCTGTCTCTTATAC11.1764705882352942No Hit
CCCGGATCGCGGCCCGGTCGCCGTCGAGCCGGAGGACTGTCTCTTATACA11.1764705882352942No Hit
GTGTTGACCGCTCCGGTCAGGAGGAGGGCCCCGCGCGGTGTCAGCGCTGT11.1764705882352942No Hit
CCGCAGCGCCATGCCGGCCTTCGCTCCGGCCGCGCGCAGCTCCCGGGCCA11.1764705882352942No Hit
TCATTAGGAGGGCTGAGAGGGCCCCTGTTAGGGGTCATGGGCTGGGTCTG11.1764705882352942No Hit
CCTAAGAACAGCTAAAAGAGCACACCCGTCTATGTAGCAAAATAGTGGGA11.1764705882352942No Hit
GTACTCGGTGGGTCCGTGGTCCACCATCTCGTAGCCAGCGGCGGTGAGGT11.1764705882352942No Hit
GGCCCAGGCGGTGGTGGCGCTGCCGCTCGCGGTGCGGGCGCTCGTGCCCG11.1764705882352942No Hit
GTATGGGATGGCGGATAGTAAGTTTGTAATTACTGTGGCCCCTCAGAATG11.1764705882352942No Hit
GACCGGGTCGGGCCCCGCGGCCCCGACGGCCTGTCTCTTATACACATCTC11.1764705882352942No Hit
GGCCAGGTGGACGCCGACGACGTCGTGCCCGGCGTCCACGGCACGCGCCG11.1764705882352942No Hit
ATCTCCGAGCCCACGAACGGTAAACCATCTCGTATGCCGTCTTCTGCTTG11.1764705882352942Illumina PCR Primer Index 7 (96% over 27bp)
GCTATGGGCAGCTCCAGAGATTCAACACCGTGGAGACTGTCTCTTATACA11.1764705882352942No Hit
ACAGTGCCCTGTGTTCAAGTTACTCTTATTTTGGTTAATAGTGACCCCAG11.1764705882352942No Hit
ATTAACTTCCTACACATCTCCGAGCCCACGACAAACGGTCATCTCGTATG11.1764705882352942No Hit
TCCTACCTCGCCGCCCGCGGCCTCAAGACGCTCGGCCTGTCTCTTATACA11.1764705882352942No Hit
TCGTGGATGTGGGTCAGGCCCGCCGCGAGCTCGCCCCTGTCTCTTATACA11.1764705882352942No Hit
AGTCTAATCCTTTTTGTAGTCACTCATAGGCCAGACTTAGGGCTGTCTCT11.1764705882352942No Hit
CGCCTGCTCCTCGAGCTCGGCGCGGCCGGCGTCGAGCTGCTCCTGGCCCT11.1764705882352942No Hit
CTGTCTCTTATACACATCTCCGAGCCCCGAGACATGATGATATCTCGTAT11.1764705882352942No Hit
GCAGGGCGCGCCCGCCGCGGCCCGCGCGTCGGACCATCCACCAGCGCTGT11.1764705882352942No Hit
GGCCACGTCCGTCCGCGGCGCGCGTGGACGAGCACGGAGAGCAGGTGCTG11.1764705882352942No Hit
GTACACCGCCGCGTCGTCGGGGCGGGCGGGGACCTGGGCCGCGTCGGCCG11.1764705882352942No Hit
ATCTCCGAGCCCACGAGACTAACAACAATCTCGTATGCCGTCTTCTGCTT11.1764705882352942RNA PCR Primer, Index 36 (100% over 28bp)
GCTGATGGTGGTCTCCAACGACACGTTCGGCTACATCGTGGGCCTGTCTC11.1764705882352942No Hit
CGCCCAGGTCATGCGCGTCATCACGCGGTCCTGGCGCTGTCTCTTATACA11.1764705882352942No Hit
AGCCCACGAGACGATCCAAAATCTCGTATGCCGTCTTCTGCTTGAAAAAA11.1764705882352942Illumina PCR Primer Index 6 (96% over 29bp)
GGCTGGACCCGGACGCGGAGCACGGCGACGGCACCGGCGAGGGCCTGTCT11.1764705882352942No Hit
CAGCAGCTAGGACTGGGAGAGATAGGAGAAGTAGGACTGCTGTGATTAGG11.1764705882352942No Hit
CCCTGGAAGTCCGGGTCGAGGCGGATGGCCGCGGCTGTCTCTTATACACA11.1764705882352942No Hit
GTAGTTCCCTGCTAAGGGAGGGTAGACTGTTCAACCTGTTCCTGCTCCGG11.1764705882352942No Hit
AACCCCAAGAGTCATATGACCTTTGCCACTTTGATTTCCTGCTTTCAATC11.1764705882352942No Hit
GTCGTGGAGCGGACCTCGCGCATCGTCACCGGCAACCCGCTCGACACAGA11.1764705882352942No Hit
ACACTAAAGGACGAACCTGATCTCTTATACTAGTATCCTTAATCATTTTT11.1764705882352942No Hit
GCCCCCGTGCGCAAGCGCTCTGAGGCCGCCGGATTTGAATCGGCGGCGTT11.1764705882352942No Hit
GCACCGGTTGGCGGCCCTTCCTCGCACCTTGTCGCCGCCCTGTCTCTATA11.1764705882352942No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTGCA50.044.011
GTGCAGC50.044.013
ATCTCCG50.044.042
CACATCT50.044.039
CAGCAGG50.044.019
CAGCAGC50.044.016
TATACAC50.044.035
TGTCTCT50.044.028
CCTGTCT50.044.026
GGCCGTG50.044.09
CATCTCC50.044.041
CTCTTAT50.044.031
CCGCAGG50.044.04
ACATCTC50.044.040
TTATACA50.044.034
TACACAT50.044.037
ACACATC50.044.038
AGCAGGC50.044.020
AGCAGCA50.044.017
CCCGCAG50.044.03
Produced by FastQC (version 0.11.5)