FastQCFastQC Report
Wed 25 May 2016
SRR1780562_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780562_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences74105
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATAATAAAGGTGGATGCGACAATGGATTTTACATAATGGGGGTATGAGTT760.10255718237635787No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTAGAC150.00221735569.994653
TCGGCTC150.00221735569.994629
AGGTCAT200.006928638652.49595338
ATGGAGT200.006928638652.4959534
TTAGACA200.006928638652.49595354
CCGACGT200.006928638652.49595323
GATAGTC200.006928638652.49595325
GAGGAAG350.001248656339.99691429
AAAGTTG350.001248656339.99691433
TAAGACC459.122969E-538.885894
AGCCCGT400.002405815334.9973036
GGTTTAA400.002405815334.99730339
TAATAGT450.00428436531.1087114
CTAAGAC450.00428436531.1087113
TCAGAAT604.942194E-429.16441761
GAGTGGA500.00717029827.997847
ATCAGTG500.00717029827.9978419
AGAAGGT500.00717029827.9978436
TCCCTAA500.00717029827.9978463
AATTGTG500.00717029827.9978443
Produced by FastQC (version 0.11.5)