##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780562_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 74105 Sequences flagged as poor quality 0 Sequence length 76 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.44157614196073 34.0 27.0 34.0 12.0 34.0 2 30.811780581607177 34.0 32.0 34.0 21.0 34.0 3 31.219256460427772 34.0 33.0 34.0 23.0 34.0 4 31.420106605492208 34.0 34.0 34.0 23.0 34.0 5 31.497712704945684 34.0 34.0 34.0 23.0 34.0 6 34.19230821132177 38.0 35.0 38.0 23.0 38.0 7 33.61924296606167 37.0 34.0 38.0 21.0 38.0 8 32.5711625396397 37.0 31.0 38.0 11.0 38.0 9 33.20282032251535 37.0 34.0 38.0 21.0 38.0 10-11 33.59439309088455 37.0 34.0 38.0 21.5 38.0 12-13 33.96355846434113 38.0 34.0 38.0 22.0 38.0 14-15 33.55343768976452 37.0 34.0 38.0 21.0 38.0 16-17 33.39122191485055 37.0 34.0 38.0 21.0 38.0 18-19 33.312637473854664 37.0 34.0 38.0 16.5 38.0 20-21 33.421300856892245 37.0 34.0 38.0 21.0 38.0 22-23 33.35304635314756 37.0 34.0 38.0 21.0 38.0 24-25 33.81765063086162 37.5 34.0 38.0 21.5 38.0 26-27 33.88518318601983 38.0 34.0 38.0 21.5 38.0 28-29 34.008811821064704 38.0 34.0 38.0 22.0 38.0 30-31 33.86909790162607 38.0 34.0 38.0 22.0 38.0 32-33 33.64571216517104 37.0 34.0 38.0 21.0 38.0 34-35 33.50471628095271 37.0 34.0 38.0 21.0 38.0 36-37 33.4529586397679 37.0 34.0 38.0 21.0 38.0 38-39 33.99493961271169 37.5 34.0 38.0 22.0 38.0 40-41 33.48152621280615 37.0 34.0 38.0 21.0 38.0 42-43 33.78927872613184 37.0 34.0 38.0 21.5 38.0 44-45 32.87330139666689 37.0 32.5 38.0 16.0 38.0 46-47 33.599284798596585 37.0 34.0 38.0 21.0 38.0 48-49 33.303400580257744 37.0 34.0 38.0 21.0 38.0 50-51 33.27234329667364 37.0 34.0 38.0 21.0 38.0 52-53 33.24248701167262 37.0 34.0 38.0 21.0 38.0 54-55 32.94533432292018 37.0 32.5 38.0 21.0 38.0 56-57 33.02196882801431 37.0 33.0 38.0 16.0 38.0 58-59 32.897867890155865 37.0 32.0 38.0 16.0 38.0 60-61 32.91950610620066 37.0 32.5 38.0 19.5 38.0 62-63 33.004331691518786 37.0 32.5 38.0 19.5 38.0 64-65 33.01948586465151 37.0 32.5 38.0 19.5 38.0 66-67 32.97835503677215 37.0 32.5 38.0 19.5 38.0 68-69 32.538755819445385 37.0 31.0 38.0 15.0 38.0 70-71 32.39877875986775 37.0 31.0 38.0 11.0 38.0 72-73 32.53265636596721 37.0 31.0 38.0 15.0 38.0 74-75 32.154220362998444 37.0 30.5 38.0 11.0 38.0 76 31.406436812630727 36.0 28.0 37.0 11.0 38.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -1.9673283285461984 1101 2 -0.17876991033486433 1101 3 0.6726157121966203 1101 4 -1.7672026650694868 1101 5 -0.7706573043246081 1101 6 -2.2583820951087787 1101 7 -0.2164403446795049 1101 8 0.20571263584074728 1101 9 -1.0997757637805634 1101 10-11 -0.34569925421855174 1101 12-13 -0.13804364424854754 1101 14-15 -0.031771377706306225 1101 16-17 -1.2551182396688532 1101 18-19 0.8998124960671845 1101 20-21 -0.35977742272627466 1101 22-23 -0.3344465992365997 1101 24-25 -1.1642885679826591 1101 26-27 -1.3482578257011255 1101 28-29 0.23087070059415282 1101 30-31 -0.7341934078742369 1101 32-33 0.8079842472979877 1101 34-35 -0.19555639342814857 1101 36-37 0.989398501443425 1101 38-39 0.37809434003764153 1101 40-41 0.7659173848638048 1101 42-43 0.41009723334188664 1101 44-45 1.122044501144586 1101 46-47 1.9320611316999816 1101 48-49 0.09087798811883374 1101 50-51 0.12283786672642805 1101 52-53 1.130054099342594 1101 54-55 0.13351424815523671 1101 56-57 0.4553490331793526 1101 58-59 0.919830210285042 1101 60-61 0.8779097617254266 1101 62-63 -0.9427489633797421 1101 64-65 0.16529708567409074 1101 66-67 -0.24356029641478472 1101 68-69 0.7100606331556492 1101 70-71 1.3763121911466882 1101 72-73 0.20648230368701803 1101 74-75 -0.7429121135353753 1101 76 -0.6013772217226965 1102 1 0.40498641553325143 1102 2 -0.18182567809651573 1102 3 -0.062265876802616305 1102 4 0.2774421019282194 1102 5 0.5296329936127506 1102 6 1.333520120322845 1102 7 1.9487850181929147 1102 8 2.3962703134572507 1102 9 1.8268246945846016 1102 10-11 0.3396483393643379 1102 12-13 -3.8767296641110462 1102 14-15 -0.8720005602884342 1102 16-17 -1.9507179340920793 1102 18-19 -0.38900339392517225 1102 20-21 0.5141110417504251 1102 22-23 -0.05757876119228911 1102 24-25 0.8865746864100075 1102 26-27 -4.685125663745435 1102 28-29 0.06789132702654399 1102 30-31 0.3749433377330931 1102 32-33 -6.261641421151211 1102 34-35 -0.07881078609430858 1102 36-37 0.6657315801294459 1102 38-39 1.0193013683034948 1102 40-41 0.5346263229845079 1102 42-43 0.15101396367037978 1102 44-45 -5.412821809015842 1102 46-47 0.09896716684131235 1102 48-49 0.38145094457414075 1102 50-51 -6.204786273838884 1102 52-53 -0.5864776042479392 1102 54-55 1.2828343398282698 1102 56-57 -0.8662705237343218 1102 58-59 1.0784398359534961 1102 60-61 0.7429823362097636 1102 62-63 -4.364964395006936 1102 64-65 0.01987309789715397 1102 66-67 0.634453454540143 1102 68-69 -6.464454263712199 1102 70-71 0.34149171750268437 1102 72-73 0.9588276054440783 1102 74-75 -6.416189424459745 1102 76 -1.7362282530443167 1103 1 -0.027328328546197156 1103 2 0.09265866109370435 1103 3 0.11832999791090515 1103 4 0.4689878111209893 1103 5 0.8017236480563454 1103 6 -0.8631439998706867 1103 7 0.5607025124633509 1103 8 1.6799983501264606 1103 9 1.2849861409813386 1103 10-11 0.7462055076862129 1103 12-13 -0.7813769775818855 1103 14-15 -0.09319994913487761 1103 16-17 -0.936546811097422 1103 18-19 -0.39733036107567443 1103 20-21 -0.31787266082151433 1103 22-23 -0.5520656468556382 1103 24-25 0.586663812969725 1103 26-27 -0.03111496855827056 1103 28-29 1.080394510117955 1103 30-31 0.53437802069719 1103 32-33 -4.3186824193686775 1103 34-35 -0.448889726761486 1103 36-37 0.3146365966815168 1103 38-39 0.9928562447995475 1103 40-41 1.3330602420066597 1103 42-43 0.7929543761990345 1103 44-45 -4.435574546474463 1103 46-47 -0.07174839210954076 1103 48-49 0.554687511928357 1103 50-51 -0.036209752321184396 1103 52-53 -0.2656601863716972 1103 54-55 0.8997047243457104 1103 56-57 1.6172537950841104 1103 58-59 0.9831635436183745 1103 60-61 1.3098145236301875 1103 62-63 1.0348700842393086 1103 64-65 1.0252970856740902 1103 66-67 -0.1111793440338289 1103 68-69 -2.72374889065388 1103 70-71 0.39678838162287633 1103 72-73 0.9507680179727274 1103 74-75 -1.5038644944877575 1103 76 0.2424323020868293 1104 1 -1.5227953615132286 1104 2 -0.21517100923596288 1104 3 0.03481351439442193 1104 4 0.23139623602941484 1104 5 -0.9885693922366947 1104 6 -0.4272007764274619 1104 7 0.6932574575182997 1104 8 1.3883774710055832 1104 9 -0.10576477476957535 1104 10-11 -0.36281463883393883 1104 12-13 -1.0779200178749306 1104 14-15 0.20879180910687722 1104 16-17 -0.5716429649435781 1104 18-19 1.0938097488144365 1104 20-21 1.219054994856144 1104 22-23 0.46485285131285536 1104 24-25 0.6219202232261338 1104 26-27 0.5712888775955776 1104 28-29 0.05088443685788491 1104 30-31 -0.5935752760061064 1104 32-33 -1.0510542142404802 1104 34-35 -0.04453990991166279 1104 36-37 -0.16418666339173882 1104 38-39 -0.19226280281949926 1104 40-41 0.8803816705780889 1104 42-43 0.7220752553199077 1104 44-45 -3.0759362680861813 1104 46-47 0.07307761521646938 1104 48-49 0.4018257903166287 1104 50-51 0.5742527018912682 1104 52-53 -0.12884699955851175 1104 54-55 -0.6132989386579482 1104 56-57 0.4402391430694621 1104 58-59 0.6406955949004285 1104 60-61 -0.6822413371756753 1104 62-63 0.44727850914772915 1104 64-65 -0.3228485187215142 1104 66-67 0.6711924508379639 1104 68-69 -3.7562580481630405 1104 70-71 -0.8377812154467179 1104 72-73 0.5130619740166864 1104 74-75 -1.2067720036452627 1104 76 -0.30857502392049696 1105 1 -1.71271038834686 1105 2 -0.5116602757833704 1105 3 -0.29828129777015633 1105 4 0.25990696948200664 1105 5 -0.16361411495583766 1105 6 -0.4710066798263881 1105 7 0.8191742732606926 1105 8 1.0248488484653322 1105 9 0.5974999837277437 1105 10-11 0.9412774899674972 1105 12-13 -1.4571798568731396 1105 14-15 -0.8142962946498287 1105 16-17 1.2925229563444347 1105 18-19 0.42758658244592596 1105 20-21 0.28932556730694614 1105 22-23 0.31399193896938726 1105 24-25 0.5258110997914258 1105 26-27 0.06423386865103708 1105 28-29 -1.1307904289739525 1105 30-31 -0.006153540764607612 1105 32-33 0.650309828693338 1105 34-35 0.8243107162063978 1105 36-37 0.8076044815098697 1105 38-39 0.737097662296776 1105 40-41 0.15581771708971814 1105 42-43 0.8108945755345758 1105 44-45 1.2992538034701653 1105 46-47 0.43843986924151324 1105 48-49 -0.1139725102200444 1105 50-51 0.19546245144404395 1105 52-53 -1.1930688242454508 1105 54-55 -1.4025654860640344 1105 56-57 -0.39647820934556677 1105 58-59 0.0765743963315515 1105 60-61 0.1603682334862242 1105 62-63 0.5455566844607915 1105 64-65 0.31964924514252857 1105 66-67 0.4547121288343874 1105 68-69 0.9676686065775684 1105 70-71 1.2606311280237676 1105 72-73 0.7001035661454864 1105 74-75 -1.4119486583858745 1105 76 -1.090015095476847 1106 1 -0.9018181244645653 1106 2 0.08653621211411533 1106 3 -0.9408536755584826 1106 4 -0.21434552221234426 1106 5 -0.08433077371236308 1106 6 -0.6655249522516371 1106 7 0.3668249614429442 1106 8 0.49326365624891366 1106 9 1.106754848464334 1106 10-11 0.17868850088348864 1106 12-13 0.324405335343279 1106 14-15 0.22924903045695544 1106 16-17 1.0777389031882834 1106 18-19 0.16950637361820498 1106 20-21 -0.6864100757874994 1106 22-23 -0.4212833339304751 1106 24-25 0.5041808197724365 1106 26-27 0.3086809498090801 1106 28-29 -0.6668844014466657 1106 30-31 -0.6941934078742378 1106 32-33 1.1311475126041088 1106 34-35 0.378831361673889 1106 36-37 0.5459291136883238 1106 38-39 0.31666576860907014 1106 40-41 0.7177541195576822 1106 42-43 0.7613217231378044 1106 44-45 1.1875547052262192 1106 46-47 0.2566529684346719 1106 48-49 0.6206739064861821 1106 50-51 0.5675317442774528 1106 52-53 -0.08096630882067757 1106 54-55 0.34392241142054303 1106 56-57 1.2206551556283287 1106 58-59 0.14319755722382155 1106 60-61 0.8994403739703287 1106 62-63 0.7005163427427092 1106 64-65 0.979888922408783 1106 66-67 0.8231743974627719 1106 68-69 1.4505708372372794 1106 70-71 0.6950877013507721 1106 72-73 0.7196455689931369 1106 74-75 -1.3019937461884368 1106 76 0.10841869664465165 1107 1 -0.9941697440114652 1107 2 -1.0627803952627808 1107 3 -0.3106346154573778 1107 4 -1.0324975536671275 1107 5 -0.9252313541280159 1107 6 -0.8480282287916125 1107 7 -0.42684663563625236 1107 8 0.9076523475052269 1107 9 -0.4117810062445244 1107 10-11 -0.2895524652277288 1107 12-13 -0.11291913572954826 1107 14-15 -1.1838290448098476 1107 16-17 -0.303978003757976 1107 18-19 0.549327568151071 1107 20-21 -0.1308259155178888 1107 22-23 -0.9744334930767025 1107 24-25 -0.5845900096602534 1107 26-27 -0.2751516658059714 1107 28-29 -1.6189064946876286 1107 30-31 -0.7685708652792229 1107 32-33 -0.1726579545368736 1107 34-35 -0.18705049565619447 1107 36-37 -0.8051755483599763 1107 38-39 -0.004762802819499257 1107 40-41 0.028604147642312228 1107 42-43 -0.4846996211797432 1107 44-45 -0.8608912786719287 1107 46-47 -0.5442036127299019 1107 48-49 0.08992123844648603 1107 50-51 0.45505280774871437 1107 52-53 -0.04698390852110634 1107 54-55 -0.35571248841094416 1107 56-57 0.47631888901158703 1107 58-59 0.05166507267036735 1107 60-61 -0.9292199892575326 1107 62-63 -0.13083546403505153 1107 64-65 -0.3891196902105776 1107 66-67 0.3845393104004202 1107 68-69 0.2489466095645554 1107 70-71 -0.3432946240564547 1107 72-73 -0.19523460325924447 1107 74-75 -0.4427679457765272 1107 76 -1.3289525821420938 1108 1 -1.251184084440787 1108 2 -0.9998517411254326 1108 3 -0.35129552220005067 1108 4 -0.4040126511998636 1108 5 -0.7116836566130971 1108 6 0.342450082422431 1108 7 0.3963474500500368 1108 8 0.6202202641347796 1108 9 0.06385807810570299 1108 10-11 0.13284443510183053 1108 12-13 0.7192933876515823 1108 14-15 0.0020843781882859957 1108 16-17 -1.020041956728491 1108 18-19 -0.24774644984127292 1108 20-21 0.08436959530423849 1108 22-23 -0.34799722336974526 1108 24-25 -0.8542746983571377 1108 26-27 -1.2320858423446737 1108 28-29 -0.015606414523745116 1108 30-31 0.768705343859466 1108 32-33 0.5300230822494427 1108 34-35 0.16135068008086506 1108 36-37 0.27244981905784726 1108 38-39 1.1020333136853537 1108 40-41 0.20289380650041977 1108 42-43 0.6643829476276011 1108 44-45 0.22029692832905567 1108 46-47 0.1309099527818134 1108 48-49 -0.4336157705496859 1108 50-51 -0.33255741760089563 1108 52-53 -0.16170734309846324 1108 54-55 0.6889788806101649 1108 56-57 1.4054183813069514 1108 58-59 -1.51424745961787 1108 60-61 0.7311968629736967 1108 62-63 -0.4138585334213474 1108 64-65 0.21544965155481322 1108 66-67 -0.15988484565195193 1108 68-69 1.147494752434426 1108 70-71 1.6973662826862181 1108 72-73 0.1615169777508143 1108 74-75 0.6980310209999914 1108 76 0.6151553718972771 1109 1 -0.7277288625915901 1109 2 -1.0752986152747859 1109 3 -0.09782487525331263 1109 4 -0.06447903356080786 1109 5 0.39874189460730136 1109 6 0.22399440689389394 1109 7 -1.8511799975499983 1109 8 0.17679407776331146 1109 9 0.8623337155251747 1109 10-11 1.0071979420431276 1109 12-13 -0.25632134785336547 1109 14-15 0.06975065166619032 1109 16-17 -1.150565502686213 1109 18-19 -0.41281767749756426 1109 20-21 0.9397819898237927 1109 22-23 0.17205540343363168 1109 24-25 0.3569130341535214 1109 26-27 0.5011280220959335 1109 28-29 0.07701222262352303 1109 30-31 -0.39679687916929396 1109 32-33 0.7942459295409776 1109 34-35 0.33751436758653597 1109 36-37 0.3318818125248697 1109 38-39 0.4298166364328324 1109 40-41 0.18032325936313498 1109 42-43 0.07559790089862872 1109 44-45 -0.3169274614722397 1109 46-47 -0.7224382007432766 1109 48-49 -0.3958242815740931 1109 50-51 0.6696202432284366 1109 52-53 1.005768385056875 1109 54-55 0.5254501627079691 1109 56-57 0.3889271373182055 1109 58-59 -0.27166511681776484 1109 60-61 0.8013810567855089 1109 62-63 -0.839250966049967 1109 64-65 0.4216922792655424 1109 66-67 0.029163335093898013 1109 68-69 0.9515960136629857 1109 70-71 -0.21731931753154754 1109 72-73 -0.47640154143982016 1109 74-75 1.1148715980801143 1109 76 -0.5322450454877163 1110 1 0.8983019235546443 1110 2 0.3030228067519687 1110 3 -0.2498772850022597 1110 4 0.09517828731146594 1110 5 0.30592532872861256 1110 6 0.07327056595564585 1110 7 0.8730274424353439 1110 8 2.1262168375214188 1110 9 -0.9543696013155767 1110 10-11 -0.33971886206168733 1110 12-13 -0.0634498067135425 1110 14-15 -0.01749966902283262 1110 16-17 0.36835514968688443 1110 18-19 0.7912550730980001 1110 20-21 -0.003937086591818684 1110 22-23 0.011939955385251722 1110 24-25 0.23862459728344732 1110 26-27 -1.1051345764014044 1110 28-29 0.3969911487734237 1110 30-31 -0.07024382804230811 1110 32-33 0.8357293453371994 1110 34-35 0.6310822620340346 1110 36-37 0.0093144678299808 1110 38-39 0.27464896188638477 1110 40-41 0.1469257882251469 1110 42-43 0.46830451625505276 1110 44-45 1.3973386187916432 1110 46-47 0.33693508127981175 1110 48-49 0.5797575399395569 1110 50-51 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-0.7964294694861707 2116 40-41 -0.6586808294219111 2116 42-43 -0.3720753857057275 2116 44-45 1.6125653344779138 2116 46-47 -0.3602156540143042 2116 48-49 -0.4279017737859263 2116 50-51 -0.5763883237497609 2116 52-53 0.25316421839020364 2116 54-55 -0.7765601566066707 2116 56-57 -1.5643682287254137 2116 58-59 -0.8767322897149583 2116 60-61 -2.1153342858936206 2116 62-63 -0.14727277290355545 2116 64-65 0.3938982761502814 2116 66-67 -0.39972101070049604 2116 68-69 -0.22040067636817184 2116 70-71 -3.18974435647236 2116 72-73 -0.2856159105987004 2116 74-75 -2.479608542106803 2116 76 -2.015305793151267 2117 1 -0.24744799945120732 2117 2 0.211656417264237 2117 3 0.30666208467231115 2117 4 -0.6356245050096518 2117 5 -0.900470318535529 2117 6 -1.9970507562905269 2117 7 -2.0653259093765897 2117 8 -3.0568752474801215 2117 9 -0.1711594586197549 2117 10-11 -0.47983537987300195 2117 12-13 0.04601020765870345 2117 14-15 -0.41919097381700254 2117 16-17 0.13177779623242003 2117 18-19 0.04634951925342534 2117 20-21 -0.4842650816641907 2117 22-23 0.6203477163954147 2117 24-25 0.36109662274284204 2117 26-27 0.5060578063108423 2117 28-29 0.32614369984620595 2117 30-31 0.08628527574580858 2117 32-33 0.9265182787115975 2117 34-35 0.17412199101462988 2117 36-37 -0.5971684394690655 2117 38-39 0.22822149037421724 2117 40-41 -1.3501484341339562 2117 42-43 -0.9350934921629772 2117 44-45 0.952620417374952 2117 46-47 -0.10485734249598266 2117 48-49 -0.1065939640128093 2117 50-51 1.0738625488505917 2117 52-53 0.9468678614966635 2117 54-55 -0.6183331714408737 2117 56-57 -0.7454737042925998 2117 58-59 -1.0703268577777862 2117 60-61 0.5804826861831671 2117 62-63 -1.0349254779646344 2117 64-65 -0.240073146188287 2117 66-67 -1.9883172148889798 2117 68-69 -0.49593787095804487 2117 70-71 0.714801345970919 2117 72-73 0.5899976365217228 2117 74-75 -0.09650972011727532 2117 76 -0.939716787915664 2118 1 -0.457306122772696 2118 2 0.3401049971410792 2118 3 -0.2011370635250671 2118 4 0.12928882271733855 2118 5 -0.823862337633269 2118 6 0.14072967395117786 2118 7 0.6687706101687567 2118 8 -5.330808459644079 2118 9 -1.37225540848819 2118 10-11 -1.0102588397107795 2118 12-13 0.8647986947595925 2118 14-15 -0.5936070549823995 2118 16-17 -0.14561416861037912 2118 18-19 -1.1533629295434693 2118 20-21 -3.3746700948210204 2118 22-23 -0.777910699902769 2118 24-25 0.15445310485227282 2118 26-27 0.6025637879184202 2118 28-29 -0.6549620159121474 2118 30-31 -0.4654739408128066 2118 32-33 -0.11574632265019602 2118 34-35 1.0168640358834722 2118 36-37 -0.7426666354921778 2118 38-39 -0.6342964815759728 2118 40-41 -1.787850194706884 2118 42-43 -0.10245643061074361 2118 44-45 -0.07888814134246047 2118 46-47 -0.4574873509054953 2118 48-49 -2.199247844597675 2118 50-51 -0.6369992909345612 2118 52-53 -1.8582212522488248 2118 54-55 0.3051944850168198 2118 56-57 1.2314408170431577 2118 58-59 1.1101929045855599 2118 60-61 -1.9619718074825698 2118 62-63 0.5990571061983516 2118 64-65 -0.8051248240224353 2118 66-67 -3.0555662179543823 2118 68-69 0.5859599669824433 2118 70-71 -0.4035693780613059 2118 72-73 -1.3671816489406687 2118 74-75 0.6360812247325782 2118 76 0.11196104622696978 2119 1 0.8018211347073105 2119 2 0.3439385950326006 2119 3 0.14508474605293742 2119 4 -0.13073693426849786 2119 5 0.8505813414227106 2119 6 1.3883561377566238 2119 7 -0.6787689986844612 2119 8 1.0866044607788155 2119 9 0.36816808427889214 2119 10-11 0.08863600589705811 2119 12-13 0.6429142417960421 2119 14-15 -0.3124815346014387 2119 16-17 -0.2073560596523336 2119 18-19 -0.7316821364679029 2119 20-21 -0.905458677887296 2119 22-23 0.39072268234887275 2119 24-25 -0.17682366294550178 2119 26-27 -0.09874502636173332 2119 28-29 0.8214074470846526 2119 30-31 0.19556712061461923 2119 32-33 0.23526748429220845 2119 34-35 0.2848564884876197 2119 36-37 -0.4253166100346917 2119 38-39 -0.5336645369235455 2119 40-41 -0.5796978091906979 2119 42-43 -1.0957326593088155 2119 44-45 -1.7541404699536827 2119 46-47 0.005075169685120784 2119 48-49 -0.6618552901635795 2119 50-51 1.2574869170980278 2119 52-53 -1.4320928866194258 2119 54-55 -2.2377987163369184 2119 56-57 0.13660419420330072 2119 58-59 1.0420267420769491 2119 60-61 0.5385373422373974 2119 62-63 -0.48710899641029215 2119 64-65 0.3841740468631869 2119 66-67 0.10018867138042964 2119 68-69 -0.5956866831119072 2119 70-71 -1.1342741011737054 2119 72-73 -2.4093063007721085 2119 74-75 -0.19772631667327545 2119 76 0.9691595247678073 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 2.0 12 32.0 13 139.0 14 377.0 15 614.0 16 787.0 17 815.0 18 842.0 19 849.0 20 701.0 21 685.0 22 641.0 23 677.0 24 792.0 25 822.0 26 946.0 27 1220.0 28 1528.0 29 1821.0 30 2215.0 31 2737.0 32 3617.0 33 4578.0 34 6319.0 35 9067.0 36 14856.0 37 16426.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 35.0088351230121 22.303928231616148 10.608943862987632 32.078292782384125 2 17.795020578908304 20.182173942379055 36.78024424802645 25.24256123068619 3 16.048849605289792 26.687807840226707 30.321840631536332 26.941501922947168 4 13.958572296066393 19.574927467782203 39.327980568112814 27.138519668038597 5 12.34194723702854 37.08116861210445 35.68450172053168 14.892382430335335 6 26.62708319276702 39.09857634437623 18.469738884015925 15.804601578840835 7 24.608326023885027 32.82099723365495 23.385736455030028 19.184940287429995 8 22.03899871803522 34.65758046015788 20.914918021725928 22.388502800080968 9 22.94312124687943 16.062343971391943 23.343903920113352 37.65063086161528 10-11 23.850617367249175 25.39504756764051 27.81661156467175 22.937723500438565 12-13 22.26907766007692 23.41542406045476 27.240402132109843 27.07509614735848 14-15 19.94062478915053 25.097496795088052 24.95310707779502 30.0087713379664 16-17 22.13818230888604 27.834828958909657 26.3153633358073 23.711625396397004 18-19 21.677349706497537 26.882126712097698 26.444234532082856 24.996289049321906 20-21 21.128803724445046 27.162809527022468 26.268133054449766 25.44025369408272 22-23 21.90607921192902 26.982659739558734 27.025166992780512 24.08609405573173 24-25 22.051818365832265 27.869239592470148 26.08056136562985 23.99838067606774 26-27 20.27258619526348 28.44612374333716 27.384117131097767 23.8971729303016 28-29 21.890560690911546 27.371297483300722 27.778152621280615 22.95998920450712 30-31 21.801497874637338 27.000877133796642 27.73429593144862 23.463329060117402 32-33 20.342756898994672 28.295661561298157 27.06632480939208 24.295256730315092 34-35 21.725929424465285 27.80784022670535 27.181026921260376 23.28520342756899 36-37 22.394575264826937 26.962418190405508 26.79778692395925 23.84521962080831 38-39 21.319749004790502 27.520410228729503 28.113487618919102 23.046353147560893 40-41 21.43917414479455 26.74380945955064 28.13912691451319 23.677889481141623 42-43 21.184805343768975 26.12239390054652 28.413062546386886 24.27973820929762 44-45 20.450711827811887 26.484042912084206 28.345590715876124 24.719654544227783 46-47 20.912893866810606 26.265434181229335 28.77875986775521 24.042912084204847 48-49 20.051953309493285 27.058902908035893 29.055394372849335 23.833749409621483 50-51 20.31981647662101 26.54206868632346 28.423858039268605 24.714256797786923 52-53 19.3711625396397 27.05687875312057 28.74907226233048 24.82288644490925 54-55 19.310437892180015 27.367249173470075 28.244382970109978 25.07792996423993 56-57 19.871803522029552 27.02449227447541 28.123608393495715 24.980095809999327 58-59 19.705148100667973 27.09398825990149 28.143849942648945 25.057013696781596 60-61 19.781391269145132 26.52250185547534 27.934012549760475 25.762094325619056 62-63 19.975035422711017 26.375413264961878 28.26192564604278 25.387625666284325 64-65 20.394035490182848 26.659469671412182 27.254571216517103 25.691923621887863 66-67 20.381890560690913 26.702651642939074 26.847041360232105 26.068416436137916 68-69 20.2482963362796 26.831522839214628 26.665542136158155 26.254638688347615 70-71 20.9830645705418 26.888873895148773 26.13049052020781 25.997571014101613 72-73 21.00330611969503 26.534646784967276 25.87679643748735 26.585250657850345 74-75 20.805613656298497 26.830173402604412 25.78638418460293 26.577828756494164 76 20.99723365494906 26.70939882599015 25.85925376155455 26.43411375750624 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.5 12 1.0 13 1.0 14 1.5 15 5.0 16 6.0 17 4.0 18 8.0 19 12.5 20 17.0 21 21.0 22 24.0 23 34.0 24 61.0 25 81.0 26 139.0 27 236.0 28 332.5 29 390.0 30 439.0 31 611.0 32 865.5 33 997.0 34 1200.0 35 1607.0 36 2027.5 37 2244.0 38 2565.0 39 3306.5 40 4048.5 41 4587.5 42 4805.0 43 4988.5 44 5366.0 45 5569.0 46 5578.0 47 5591.0 48 5373.5 49 4897.0 50 4651.0 51 4366.5 52 3683.5 53 2946.5 54 2608.0 55 2420.0 56 1931.0 57 1443.5 58 1257.0 59 1048.5 60 716.5 61 474.5 62 356.0 63 308.5 64 227.0 65 163.5 66 111.5 67 89.0 68 78.0 69 58.5 70 45.0 71 40.0 72 36.5 73 27.0 74 20.5 75 20.0 76 15.0 77 9.5 78 7.5 79 6.0 80 7.0 81 8.5 82 6.5 83 4.0 84 3.0 85 2.0 86 2.5 87 3.0 88 2.5 89 1.5 90 1.0 91 0.5 92 0.0 93 1.0 94 1.5 95 1.0 96 1.0 97 1.5 98 2.5 99 2.5 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.7219485864651508 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 74105.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.822211726604145 #Duplication Level Percentage of deduplicated Percentage of total 1 75.7519814896864 41.528911679373856 2 9.242849406783833 10.134268942716416 3 4.876187663072909 8.019701774509143 4 2.9119283217643877 6.385534039538493 5 1.9593363855658938 5.370757708656636 6 1.2356618913995965 4.064503069968288 7 0.8861320336730172 3.4005802577423925 8 0.622753901442426 2.731259699075636 9 0.5562939989169498 2.744754065177788 >10 1.9544134298232658 15.51717158086499 >50 0.002461477871313937 0.10255718237635787 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source ATAATAAAGGTGGATGCGACAATGGATTTTACATAATGGGGGTATGAGTT 76 0.10255718237635787 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.016193239322582823 0.0 2 0.0 0.0 0.0 0.018892112543013293 0.0 3 0.0 0.0 0.0 0.022940422373658998 0.0 4 0.0 0.0 0.0 0.026988732204304703 0.0 5 0.0 0.0 0.0 0.031037042034950408 0.0 6 0.0 0.0 0.0 0.031037042034950408 0.0 7 0.0 0.0 0.0 0.031037042034950408 0.0 8 0.0 0.0 0.0 0.03643478847581135 0.0 9 0.0 0.0 0.0 0.07556845017205317 0.0 10 0.0 0.0 0.0 0.09176168949463599 0.0 11 0.0 0.0 0.0 0.10525605559678834 0.0 12 0.0 0.0 0.0 0.10660549220700358 0.0 13 0.0 0.0 0.0 0.10795492881721881 0.0 14 0.0 0.0 0.0 0.12414816813980163 0.0 15 0.0 0.0 0.0 0.1308953511908778 0.0 16 0.0 0.0 0.0 0.1457391539032454 0.0 17 0.0 0.0 0.0 0.15653464678496729 0.0 18 0.0 0.0 0.0 0.1646312664462587 0.0 19 0.0 0.0 0.0 0.1727278861075501 0.0 20 0.0 0.0 0.0 0.1916199986505634 0.0 21 0.0 0.0 0.0 0.1997166183118548 0.0 22 0.0 0.0 0.0 0.21051211119357668 0.0 23 0.0 0.0 0.0 0.2186087308548681 0.0 24 0.0 0.0 0.0 0.22265704068551379 0.0 25 0.0 0.0 0.0 0.2334525335672357 0.0 26 0.0 0.0 0.0 0.24424802644895757 0.0 27 0.0 0.0 0.0 0.26314013899197086 0.0 28 0.0 0.0 0.0 0.2847311247554146 0.0 29 0.0 0.0 0.0 0.31711760340058026 0.0 30 0.0 0.0 0.0 0.3454557722151002 0.0 31 0.0 0.0 0.0 0.38593887052155723 0.0 32 0.0 0.0 0.0 0.42102422238715337 0.0 33 0.0 0.0 0.0 0.45206126442210376 0.0 34 0.0 0.0 0.0 0.5181836583226503 0.0 35 0.0 0.0 0.0 0.5924026718844883 0.0 36 0.0 0.0 0.0 0.7179002766345051 0.0 37 0.0 0.0 0.0 0.9203157681667904 0.0 38 0.0 0.0 0.0 1.2752175966533972 0.0 39 0.0 0.0 0.0 1.7313271709061466 0.0 40 0.0 0.0 0.0 2.2292692800755685 0.0 41 0.0 0.0 0.0 2.7676944875514473 0.0 42 0.0 0.0 0.0 3.318264624519263 0.0 43 0.0 0.0 0.0 3.9295594089467647 0.0 44 0.0 0.0 0.0 4.585385601511369 0.0 45 0.0 0.0 0.0 5.160245597463059 0.0 46 0.0 0.0 0.0 5.770190945280345 0.0 47 0.0 0.0 0.0 6.39767896903043 0.0 48 0.0 0.0 0.0 7.042709668713313 0.0 49 0.0 0.0 0.0 7.601376425342419 0.0 50 0.0 0.0 0.0 8.145199379259159 0.0 51 0.0 0.0 0.0 8.72545712165171 0.0 52 0.0 0.0 0.0 9.313811483705553 0.0 53 0.0 0.0 0.0 9.80770528304433 0.0 54 0.0 0.0 0.0 10.397409081708387 0.0 55 0.0 0.0 0.0 10.979016260711154 0.0 56 0.0 0.0 0.0 11.498549355644018 0.0 57 0.0 0.0 0.0 12.04777005600162 0.0 58 0.0 0.0 0.0 12.598340192969435 0.0 59 0.0 0.0 0.0 13.03015990823831 0.0 60 0.0 0.0 0.0 13.545644693340531 0.0 61 0.0 0.0 0.0 14.088118210647055 0.0 62 0.0 0.0 0.0 14.549625531340665 0.0 63 0.0 0.0 0.0 15.074556372714392 0.0 64 0.0 0.0 0.0 15.503677214762837 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTTAGAC 15 0.002217355 69.9946 53 TCGGCTC 15 0.002217355 69.9946 29 AGGTCAT 20 0.0069286386 52.495953 38 ATGGAGT 20 0.0069286386 52.495953 4 TTAGACA 20 0.0069286386 52.495953 54 CCGACGT 20 0.0069286386 52.495953 23 GATAGTC 20 0.0069286386 52.495953 25 GAGGAAG 35 0.0012486563 39.996914 29 AAAGTTG 35 0.0012486563 39.996914 33 TAAGACC 45 9.122969E-5 38.88589 4 AGCCCGT 40 0.0024058153 34.997303 6 GGTTTAA 40 0.0024058153 34.997303 39 TAATAGT 45 0.004284365 31.108711 4 CTAAGAC 45 0.004284365 31.108711 3 TCAGAAT 60 4.942194E-4 29.164417 61 GAGTGGA 50 0.007170298 27.99784 7 ATCAGTG 50 0.007170298 27.99784 19 AGAAGGT 50 0.007170298 27.99784 36 TCCCTAA 50 0.007170298 27.99784 63 AATTGTG 50 0.007170298 27.99784 43 >>END_MODULE