FastQCFastQC Report
Wed 25 May 2016
SRR1780562_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780562_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences74105
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGT8691.1726604142770392No Hit
ATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGTCTT5160.6963092908710613RNA PCR Primer, Index 42 (95% over 22bp)
ATAAAGAATATTGAGGCGCCATTGGCGTGAAGGTAGCGGATGATTCAGCC2310.3117198569597193No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCC1020.137642534241954No Hit
TCCTTGTACTATCCCTATGAGGCATAATTATAACAAGCTCCATCTGCCTA860.11605154847851022No Hit
CTTATACACATCTCCGAGCCCACGAGACATCACTCGATATCGTATGCCGT840.11335267525807975No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGTCT207.879847E-570.055
GTCTCGA301.01110345E-770.060
CGTCTCG301.01110345E-770.059
TCTCGAG301.01110345E-770.061
CTCGAGT301.01110345E-770.062
CTCTAGC150.00221667770.02
CGGATGA401.0490112E-861.2537
ATGATTC352.9378498E-759.99999640
TTCAGCC352.9378498E-759.99999644
ACGTCTC352.9378498E-759.99999658
TCGAGTG352.9378498E-759.99999663
CGAGTGA308.303303E-658.33333264
TCCGGCG252.376418E-455.99999622
TGCGGTC252.376418E-455.99999654
CGGGGTC252.376418E-455.99999627
TCTGTTA252.376418E-455.99999659
GGTTGCG252.376418E-455.99999651
GTTGCGG252.376418E-455.99999652
GCGGATG452.6577254E-854.44444336
CGCCATT452.6577254E-854.44444317