FastQCFastQC Report
Wed 25 May 2016
SRR1780561_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780561_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences71
Sequences flagged as poor quality0
Sequence length50
%GC61

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACATCTACCAGTGTCCTCTTGCAAGCCCCGCCCCAGGGCTCTCCCTCGGT11.4084507042253522No Hit
CCACAGCCCCAGCCCGAGCACCATGAGCGCCTGTCTCTTATACACATCTG11.4084507042253522No Hit
GTTGTTCCCACTGTGTTGTGTGATTGTGGCTGCTATGTTGCTGTTGTGCT11.4084507042253522No Hit
CCAGGAGGGCTTGTCGCCGTCGGTGCCGGGCTTGTTCACGCCTGTCTCTT11.4084507042253522No Hit
CCGGTGACCACCGCAGACCCCGGCTTCGCCCTGCCCTCCGGCTTCGAACC11.4084507042253522No Hit
GGACTCGATGGTGGTGATCATGCACGCCGGTGCGCAGGGGCCGGTGGCTG11.4084507042253522No Hit
ACCCTCGGGGGACTGGCGCACGCGCACGACCTCCTGTCTCTTATACACAT11.4084507042253522No Hit
CGCCGCCACATCGCGCGGAACGCCCAGAAGACCATCTGTCTCTTATACAC11.4084507042253522No Hit
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCT11.4084507042253522No Hit
CTTCGAGGTCGCCGCCGTCACGCGGCCGGGCTACCCCGTGCCTGTCTCTT11.4084507042253522No Hit
CCCAGATGCCGCGCAGCATGGACGGCCACGGCTCGCGCACCACGAGCTGT11.4084507042253522No Hit
CATCTGCACCGATCTGCCGGCAGAACTCAGCCAGCAGGCTGTCTCTTATA11.4084507042253522No Hit
CTCCTTCGTCGTGCAGCATCAGCTCCATCCCGCCGAGCTCGCCGCGTTCG11.4084507042253522No Hit
GTCGATCACGACGCGGCCGGCCGCGTCGTGCGTGCCGTGGGCGCAGGCGA11.4084507042253522No Hit
GGCTGGGATCCTCGAGCGCCTCGAGATCTACCGCGACCACGGGTCTGTCT11.4084507042253522No Hit
GTTCTCGAACACCGCGACGGTCGGGTTGCCCGTGCGGCCTGTCTCTTATA11.4084507042253522No Hit
GTGGACCGCCGTGCCGTGGTGCAGGCGCGCGTGGTCCACACGCTGTCTCT11.4084507042253522No Hit
GTGCTGGGATTACAGCGTGAGGCACCGCACCCAGCCTATGTCACTCTTTA11.4084507042253522No Hit
GGTGGGCGTCGCCGTCGTCGGCGGCCCCCGCGTCGGCCCGCCGGGCCGCG11.4084507042253522No Hit
GCGTAGCGCAGGCCGGCGATCCGCTGGTACGCCACGGCGTGGGAGGAGAC11.4084507042253522No Hit
CACCACCAGTACGCCCAGCGTGGAGATGGCGATGACCACTTCAGAAGCTG11.4084507042253522No Hit
CGCGTGTTCCAGCCCATGGAGATGGTGGCGATGTTCTACTCCTGTCTCTT11.4084507042253522No Hit
ACGTCGACCCGCGACCGACGTCCTCCGGCGTGCGCCCGCGCATCCCCCCA11.4084507042253522No Hit
CTCCTCGGGCGCTGCCGGGCGCCCGCGCACCAGGGCGCCGAGGCGGCTGT11.4084507042253522No Hit
GTACTCATCTTGCGCTGCCGTCGACCGTGCCTTCGGGGTTATTGGTTATG11.4084507042253522No Hit
GTGCAACTCCAAATAAAAGTAATAACCATGCACACTACTATAACCCTGTC11.4084507042253522No Hit
CAGTTATTATCTTGGCTTCATCAGCCTGAAATTGCAGAAATATGCATTTA11.4084507042253522No Hit
ACCTTGGAGATCGCCGTGGTGTCCACGACGACGCCGGCCAGGCCCTTCTT11.4084507042253522No Hit
CTTATACACATCTGACGCTGCCGACGAGAATGGTCGTGTGGAGATCGGGG11.4084507042253522No Hit
GAACACACAATAGCTAAGACCCAAACTGGGATTAGATACCCCACTATGCT11.4084507042253522No Hit
AGGGTGTTGTGAGTGTAAATCAGTGCGATGAGTAGGGGAAGGGAGCCTGT11.4084507042253522No Hit
CTCCGGCGCCGATCGCGGCCGCGCCGACCCAGGCCCCCATGGCGTTGGCC11.4084507042253522No Hit
GTCTGGGGCATGCGCCCATGGTAGGACCGACCCCGGACCTGTCTCTTATA11.4084507042253522No Hit
CGCCGAAGAAGCGGCTATCCATTTCGTCATCGACGAGGGCGACTATGACC11.4084507042253522No Hit
GTCAGAAGTAAAAGGAGCCTGACAAATGAGGCTTGAATTTTTTTTCCAGT11.4084507042253522No Hit
AACAATGACTAATCAAACTAACCTCAAAACAAATAATAACCATACACAAC11.4084507042253522No Hit
CTTAACCACATCCCACGCTGCCGAGAAGTGTCACGGGGTAGATCCCGGGG11.4084507042253522No Hit
ACGTAGGACTTTAATCGTTGAACAAACGAACCTTTAATAGCGGCTGCACC11.4084507042253522No Hit
ACCCCATCTCTACTAAAAATACAAAAAATTAGCCAGGCATGGTGGCACAT11.4084507042253522No Hit
CTATAGGGCTGGTGCGACACCTGCTGTTCTTTTATGGAATTGCGTAGGCC11.4084507042253522No Hit
GTCGTCGTGAAGGACACCCCCGCCGACGCGATCGCCACCGGCGTGCCGGC11.4084507042253522No Hit
GTCTACGGAGGCTCCAGGGTGGGAGTAGTTCCCTGCTAAGGGAGGGTAGA11.4084507042253522No Hit
GTCGAGCGTGCGACGGGCGCTGTGCTCGGCCGCAGTGTGGGGAACAACTT11.4084507042253522No Hit
GTCGCCGATCTGCGTCGTGCGGGTGGGCCAGTCCGGCACCTGTCTCTTAT11.4084507042253522No Hit
GGGATGGACGGCGTGACCGCCACCCGCGCGGTGCGGGAGCGGTGGCCGGG11.4084507042253522No Hit
CCTGTAATCTCAGCACTTTGAGAGGCTAAGGCGGGTGGATCACCTGAGGT11.4084507042253522No Hit
CGCCTCGCTGTGGGCGCTGGACGGCTTCGGCCCGACGACGGACGCCGCGT11.4084507042253522No Hit
GGTGGCCACCGTGGGCCGGCTGACGCGGAACTCGGCGGCCACGTGTGCCT11.4084507042253522No Hit
TCCGGCAGGCGCCAAGGAGCGAGCCGTAGGTTCATAATACGGAGATGTTT11.4084507042253522No Hit
GGCGTTGACGGCGGCGTCGTACTGCAGCGGGCCCTCCACCTTCAGCCTGT11.4084507042253522No Hit
GCACCAGACCGCGGCCCGCGCCCGTGAGATCTGGCTGCCGGAGCGCTGTC11.4084507042253522No Hit
CCAATGCTAACCACAATGGCATTCCTCATGCGTAACGACCGAACCATTCT11.4084507042253522No Hit
GGGAGGGCACGCCCGTGCGGCTCGTGCCTGTCTCTTATACACATCTGACG11.4084507042253522No Hit
CGTGGGAGCCGCCCCCGTGCGCGACCTGGACCATCGGGTCGCTGTCTCTT11.4084507042253522No Hit
GGTCGCCGCGGCCGCCGACCGCCAGGCCCAACGCTGGGCCTGTCTCTTAT11.4084507042253522No Hit
TCTTATACCCACCAGACGCTGCCGACGAACTTCTAGGTGTCTATGTCGGG11.4084507042253522No Hit
GCTGCAGTGGGAGGCCTTCACCCGCGCCCTGGAGGGCATCTGTCTCTTAT11.4084507042253522No Hit
ACGGAAATGTTCACTAGATGGTGAAATTATGGATTTTTTTATTGTTTTGT11.4084507042253522No Hit
CCTATGAAAAAACTTCCTACCACTCACCCTAGCATTACTTATATGACATG11.4084507042253522No Hit
GCATTATATAGTAGCAAATAAAGTGAAGATTTATGCTAGAACCTAGGAGG11.4084507042253522No Hit
GTCCACGATGACGCCGATCCCCGCCCGGTGGAGCGCCTGTCTCTTATACA11.4084507042253522No Hit
GACTGGCGCTGACCGACGCGGGCGTGCCGGGGCCGGAGGTGAATCCGCTG11.4084507042253522No Hit
GTACCGCCTGGGCCGCCTGCCGCTGGAGGAGTTCGTCACGGAGACCATCG11.4084507042253522No Hit
GGGTATTACTTTCCAAATTCCTCTAGCCATTCCTTAAAAGTTAGCTGTTC11.4084507042253522No Hit
GTCCACAGGCGGCCGCGGGGGCGTCCCCGTCTCTGGCAGGATCCGGGCAT11.4084507042253522No Hit
GCGCCGCGGCACCACCGTCACCGGCGCGGACCTCGCCGCTGTCTCTTATA11.4084507042253522No Hit
GAAGTCAGGTGGCCTGCTCTTCCAGACAGAAACTGGAACTGAGAGGGCTG11.4084507042253522No Hit
GTCGTCGACAGCCATCTGCGCAGCACCAACCCGCGCGTCTGGGCCTGTCT11.4084507042253522No Hit
GGAGATGGGCATGAGGGGGCGGCGTAACGAGAGAAAGAACGTCTAAAAGC11.4084507042253522No Hit
CTTGTCCCCGATCTGCTCACGGATGCGCTCGACCTGCTCCTCGATCACGC11.4084507042253522No Hit
ATGCGCAACAGGGGCTCGGTGACCTCCAGGGCCTCCTGCAGCTCCCGCAG11.4084507042253522No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCCAG50.044.030
AGCCCCG50.044.024
TCCTCTT50.044.014
TTGCAAG50.044.019
GTGTCCT50.044.011
CCCAGGG50.044.032
CTCCCTC50.044.041
GCAAGCC50.044.021
TGTCCTC50.044.012
CCAGTGT50.044.08
CGCCCCA50.044.029
TGCAAGC50.044.020
CCTCGGT50.044.044
ATCTACC50.044.03
CCGCCCC50.044.028
CATCTAC50.044.02
AGGGCTC50.044.035
CTCTTGC50.044.016
CTCTCCC50.044.039
ACATCTA50.044.01
Produced by FastQC (version 0.11.5)