FastQCFastQC Report
Wed 25 May 2016
SRR1780561_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780561_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences71
Sequences flagged as poor quality0
Sequence length50
%GC61

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCCCGTGCTGCACGTGATCGCCTGCGCCCACGGCACGCACGACGCGGCC11.4084507042253522No Hit
GCCGCACGGGCAACCCGACCGTCGCGGTGTTCGAGAACCTGTCTCTTATA11.4084507042253522No Hit
GCGTGAACAAGCCCGGCACCGACGGCGACAAGCCCTCCTGGCTGTCTCTT11.4084507042253522No Hit
GCAGCAGGGGCAAGCTCTGACAGACTGGAAAAAAAATTCAAGCCTCATTT11.4084507042253522No Hit
GTCCTCCTGGGGGGATGCGCGGGCGCACGCCGGAGGACGTCGGTCGCGGG11.4084507042253522No Hit
GCATCGGCCTACGCAATTCCATAAAAGAACAGCAGGTGTCGCACCAGCCC11.4084507042253522No Hit
CACCAACACCGCCCGAGCCCACGAGACTAACAACAATCTCGTATGCCGTC11.4084507042253522No Hit
GATTACAGGTGTGAGCCACCACGCCTGGCCTCTTTCCAAATAGATTTGGA11.4084507042253522No Hit
GCTGGGATTACAGGCATGTGCCACCATGCCTGGCTAATTTTTTGTATTTT11.4084507042253522No Hit
GCACGGGGTAGCCCGGCCGCGTGACGGCGGCGACCTCGAAGCTGTCTCTT11.4084507042253522No Hit
GCCCAGACGCGCGGGTTGGTGCTGCGCAGATGGCTGTCGACGACCTGTCT11.4084507042253522No Hit
CGTCAGGACAACTCGGCCACAGCGAGAATGCCAGCAACGCATCATGATCA11.4084507042253522No Hit
CAAATCTCCGAGCCCACGAGACATGATGATATCTCGTATGCCGTCTTCTG11.4084507042253522TruSeq Adapter, Index 25 (100% over 24bp)
GCAGAAGCAGCGGATTCACCTCCGGCCCCGGCACGCCCGCGTCGGTCAGC11.4084507042253522No Hit
GCCCTGCGGGAGCTGCAGGAGGCCCTGGAGGTCACCGAGCCCCTGTTGCG11.4084507042253522No Hit
GCTCCCTTCCCCTACTCATCGCACTGATTTACACTCACAACACCCTCTGT11.4084507042253522No Hit
CCACCGGCCCCTGCGCACCGGCGTGCATGATCACCACCATCGAGTCCCTG11.4084507042253522No Hit
ACTCCTGCTCGCATCTACTATAGTGGAGGCCGGAGCAGGAACAGGTTGAA11.4084507042253522No Hit
CGGTAATCCTTGTCGCCGTGGATGACGAGCATCGGGGAGACGATCTCGCC11.4084507042253522No Hit
CCGCCTCGGCGCCCTGGTGCGCGGGCGCCCGGCAGCGCCCGAGGAGCTGT11.4084507042253522No Hit
GCGCTCATGGTGCTCGGGCTGGGGCTGTGGCTGTCTCTTATACACATCTC11.4084507042253522No Hit
CTCGTGGTGCGCGAGCCGTGGCCGTCCATGCTGCGCGGCATCTGGGCTGT11.4084507042253522No Hit
GTTCTCGCAGAGCATCGTGCGGATCGTCTCCACGCGGTCACGTGGATCGA11.4084507042253522No Hit
CGCTCCGGCAGCCAGATCTCACGGGCGCGGGCCGCGGTCTGGTGCCTGTC11.4084507042253522No Hit
TCTTATACACATTTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCCG11.4084507042253522No Hit
GTCGAGGCCCAGGTAGGAGCCGTCCGCGAGGATCAGCGTGGTCCTGCCCT11.4084507042253522No Hit
GCCTGCAGCTCCGGCTCCGTGAAGGGGTCGCCCGCCAGCACGAGTGCCCC11.4084507042253522No Hit
GTCCAGGGCGATGGTCTCCGTGACGAACTCGTCCAGCGGCAGGCCGCCCA11.4084507042253522No Hit
ATGGTCTTCTGGGCGTTCCGCGCGATGTGGCGGCGCTGTCTCTTATACAC11.4084507042253522No Hit
AGACCACGGCGTTCCGTTTCCCGGACCACGCCACCTGTCTCTTATATACA11.4084507042253522No Hit
ACCCGTGGTCGCGGTAGATCTCGAGGCGCTCGAGGATCCCAGCCCTGTCT11.4084507042253522No Hit
ATCAGCACCTGCACCCCCGGCCACCGCTCCCGCACCGCGCGGGTGGCGGT11.4084507042253522No Hit
ACCTCCGCCTCCCAGGTTCAAGCAATTCTCCTGCCTCAGCCTCCTGAGTA11.4084507042253522No Hit
GAACCAGAGTGTAGCTTAACACAAAGCACCCAACTTACACTTAGGAGATT11.4084507042253522No Hit
GAGATCAGGTTCGTCCTTTAGTGTTGTGTATGGTTATTATTTGTTTTGAG11.4084507042253522No Hit
ATTCATCACATTATATTATTAAATTTTTATGAGTAAAAATAGGCATTGCA11.4084507042253522No Hit
CCCTCTCAGTTCCAGTTTCTGTCTGGAAGAGCAGGCCACCTGACTTCCTG11.4084507042253522No Hit
GAGGAGGGACACCATGACCGAGACCGAGATCAAGAAGGGCCTGGCCGGCG11.4084507042253522No Hit
ATGGAGGTCTCCTCCCACGCCGTGGCGTACCAGCGGATCGCCGGCCTGCG11.4084507042253522No Hit
GTCCGGGGTCGGTCCTACCATGGGCGCATGCCCCAGACCTGTCTCTTATA11.4084507042253522No Hit
GCGCTCCACCGGGCGGGGATCGGCGTCATCGTGGACCTGTCTCTTATACA11.4084507042253522No Hit
GCACCACGGAGAAGTCGTGGTCGCGGACGTAGGAGGAGAAGTTGTTCCCC11.4084507042253522No Hit
CTTCTGAAGTGGTCATCGCCATCTCCACGCTGGGCGTACTGGTGGTGCTG11.4084507042253522No Hit
GCACGAGCCGCACGGGCGTGCCCTCCCCTGTCTCTTATACACATCTCCGA11.4084507042253522No Hit
AAGCTGGAGTGCAATGGCGCAATCTCAGCTCACTGCGACCTCTACCTCCT11.4084507042253522No Hit
CATTAACACTATTCTCACCAGACCTCCTAGGCGACCCAGACAATTATACC11.4084507042253522No Hit
CGTGTGGACCACGCGCGCCTGCACCACGGCACGGCGGTCCACCTGTCTCT11.4084507042253522No Hit
GCCCAGCGTTGGGCCTGGCGGTCGGCGGCCGCGGCGACCCTGTCTCTTAT11.4084507042253522No Hit
ATGAGAGAGTCTTTTAAAGAGTGACATAGGCTGGGTGCGGTGCCTCACGC11.4084507042253522No Hit
CGACCCGATGGTCCAGGTCGCGCACGGGGGCGGCTCCCACGCTGTCTCTT11.4084507042253522No Hit
GTCCACCGCCACCTGCACGACGGGATCCCGCAGGCACACGTGGCCGCCGA11.4084507042253522No Hit
GGATAACAGCGCAATCCTATTCTAGAGTCCATATCAACAATAGGGTTTAC11.4084507042253522No Hit
GACATCGACACCGCGAACTCACGACCTCAGATACTCTGAAATGGCCCCTC11.4084507042253522No Hit
GTGCCGGACTGGCCCACCCGCACGACGCAGATCGGCGACCTGTCTCTTAT11.4084507042253522No Hit
GCTGAAGGTGGAGGGCCCGCTGCAGTACGACGCCGCCGTCAACGCCCTGT11.4084507042253522No Hit
GTTCGAAGCCGGAGGGCAGGGCGAAGCCGGGGTCTGCGGTGGTCACCGGC11.4084507042253522No Hit
ATGCCCTCCAGGGCGCGGGTGAAGGCCTCCCACTGCAGCCTGTCTCTTAT11.4084507042253522No Hit
GGTTATAGTAGTGTGCATGGTTATTACTTTTATTTGGAGTTGCACCTGTC11.4084507042253522No Hit
CGGCGAGGTCCGCGCCGGTGACGGTGGTGCCGCGGCGCCTGTCTCTTATA11.4084507042253522No Hit
GAATTTTGGATTCTCAGGGATGGGTTCGATTCTCATAGTCCTAGAAATAA11.4084507042253522No Hit
GAGTAGAACATCGCCACCATCTCCATGGGCTGGAACACGCGCTGTCTCTT11.4084507042253522No Hit
AAATTATAACAAACTCTCTCTCCGAGCCCACGAGACGGTAAACCATCTCG11.4084507042253522No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTATGCCGT11.4084507042253522No Hit
CCTGCACGTGGGTGCGGGCCTGGGCGCGGACTGGACGGCCAGCGGCGTGA11.4084507042253522No Hit
CACGTACTCCGCGGGGTCCACGAGCGGTTTGCCCTCGGCCGGGCCCTTGC11.4084507042253522No Hit
CCTGCTGGCTGAGTTCTGCCGGCAGATCGGTGCAGATGCTGTCTCTTATA11.4084507042253522No Hit
GAACATGGCCAACGCCATGGGGGCCTGGGTCGGCGCGGCCGCGATCGGCG11.4084507042253522No Hit
GATTTAACTGTTGAGGTTTAGGGCTAAGCATAGTGGGGTATCTAATCCCA11.4084507042253522No Hit
GAGGTCGTGCGCGTGCGCCAGTCCCCCGAGGGTCTGTCTCTTATACACAT11.4084507042253522No Hit
GGCTGGACGGACCTGCACGTGGGCGACCCGGCCGGCGACGTCGCCTGGCT11.4084507042253522No Hit
AGGCATACCAGGTTGGAGAGGTGCTCCTGTTCTAAGCAAATTGACTCACC11.4084507042253522No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATGAT50.044.024
AGCCCAC50.044.011
CATGATG50.044.022
GTATGCC50.044.036
AATCTCC50.044.03
ATCTCGT50.044.031
ATCTCCG50.044.04
AAATCTC50.044.02
GCCCACG50.044.012
ACATGAT50.044.021
TGCCGTC50.044.039
GATATCT50.044.028
ATGATGA50.044.023
ATGATAT50.044.026
CCACGAG50.044.014
TATGCCG50.044.037
CCGTCTT50.044.041
GAGCCCA50.044.010
CACGAGA50.044.015
CCCACGA50.044.013