FastQCFastQC Report
Wed 25 May 2016
SRR1780560_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780560_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences190194
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTTACG207.916351E-569.9915858
TAAACCG150.002223102169.9915857
CCCGAAC150.002223102169.99158540
CAGGGCA200.006946464552.4936919
AGCGCAG200.006946464552.4936962
GGTATTG200.006946464552.4936933
GGTATGA200.006946464552.4936956
ACTCGTC200.006946464552.4936915
GGGCACG200.006946464552.4936921
CGCCGGC200.006946464552.4936918
AACAGTG200.006946464552.493696
TGTAAAC200.006946464552.493695
CCCGCGT200.006946464552.4936913
GGTTGCG305.8704434E-446.66105751
ACAAATG551.3061253E-744.540124
AGCATGA404.5835346E-543.744745
TAGTGTC404.5835346E-543.7447456
TGTGACG503.4683962E-641.99495338
ATAACGT503.4683962E-641.99495345
TCCGGCG350.001253869239.9951922
Produced by FastQC (version 0.11.5)