##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780560_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 190194 Sequences flagged as poor quality 0 Sequence length 76 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.26426701157765 34.0 31.0 34.0 12.0 34.0 2 31.749823864054598 34.0 33.0 34.0 23.0 34.0 3 31.853775618578926 34.0 34.0 34.0 23.0 34.0 4 32.13321661040832 34.0 34.0 34.0 27.0 34.0 5 32.18140950818638 34.0 34.0 34.0 27.0 34.0 6 34.907783631449995 38.0 36.0 38.0 27.0 38.0 7 34.27377309484 38.0 34.0 38.0 24.0 38.0 8 33.11715406374544 37.0 34.0 38.0 21.0 38.0 9 33.90091695847398 37.0 34.0 38.0 23.0 38.0 10-11 34.35440655330873 37.5 34.5 38.0 25.0 38.0 12-13 34.766375385133074 38.0 35.5 38.0 26.0 38.0 14-15 34.32312533518407 38.0 34.0 38.0 24.5 38.0 16-17 34.21456512823748 38.0 34.0 38.0 24.0 38.0 18-19 34.188281438951805 37.5 34.5 38.0 24.0 38.0 20-21 34.28737236716195 38.0 34.0 38.0 24.0 38.0 22-23 34.28708844653354 37.5 34.5 38.0 24.0 38.0 24-25 34.69663080854286 38.0 35.5 38.0 26.0 38.0 26-27 34.77747720748289 38.0 36.0 38.0 26.5 38.0 28-29 34.92839416595687 38.0 36.0 38.0 27.0 38.0 30-31 34.77078404155757 38.0 36.0 38.0 26.0 38.0 32-33 34.478004037982274 38.0 35.0 38.0 25.0 38.0 34-35 34.33330704438626 37.0 34.5 38.0 24.0 38.0 36-37 34.32711599735008 37.5 34.5 38.0 24.5 38.0 38-39 34.910956707361954 38.0 36.0 38.0 27.0 38.0 40-41 34.37511961470919 37.5 34.5 38.0 24.5 38.0 42-43 34.706123747331674 38.0 35.5 38.0 26.5 38.0 44-45 33.760428825304686 37.0 34.0 38.0 22.5 38.0 46-47 34.50278399949525 37.0 35.0 38.0 26.0 38.0 48-49 34.22165262836893 37.0 34.0 38.0 24.0 38.0 50-51 34.19154389728382 37.0 34.0 38.0 24.5 38.0 52-53 34.19756932395344 37.0 34.0 38.0 24.0 38.0 54-55 33.89375059150131 37.0 34.0 38.0 23.5 38.0 56-57 34.018623090107994 37.0 34.0 38.0 24.0 38.0 58-59 33.86746690221563 37.0 34.0 38.0 23.5 38.0 60-61 33.90928735922269 37.0 34.0 38.0 23.5 38.0 62-63 34.02026877819489 37.0 34.0 38.0 24.0 38.0 64-65 34.032054113168655 37.0 34.0 38.0 24.0 38.0 66-67 33.92059686425439 37.0 34.0 38.0 23.5 38.0 68-69 33.51457985004785 37.0 33.5 38.0 22.5 38.0 70-71 33.35188544328423 37.0 33.0 38.0 22.0 38.0 72-73 33.50023397162897 37.0 33.0 38.0 22.5 38.0 74-75 33.08014448405312 37.0 32.0 38.0 21.0 38.0 76 32.33057299389045 36.0 31.0 38.0 20.0 38.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -1.8558544490694366 1101 2 -0.6752638569952474 1101 3 0.17897901762663437 1101 4 -2.948388531823298 1101 5 -0.6554732445054725 1101 6 -2.17312086279631 1101 7 -1.6966122971648332 1101 8 -0.1502763171712047 1101 9 -0.8354308274477447 1101 10-11 -1.0934995792594364 1101 12-13 -1.5234054651210656 1101 14-15 -0.3111617033089473 1101 16-17 -2.255634206398618 1101 18-19 -0.20525914099597742 1101 20-21 -0.718827035392529 1101 22-23 -0.828340265671315 1101 24-25 -1.0239339110608867 1101 26-27 -1.4104389992629365 1101 28-29 -0.32162896328394197 1101 30-31 -1.6665361041497277 1101 32-33 -0.24531004749206886 1101 34-35 -1.2884943607589605 1101 36-37 0.10149631837171569 1101 38-39 -0.7168307463837209 1101 40-41 0.28251133889929747 1101 42-43 -0.42071648070526635 1101 44-45 0.07177816516233548 1101 46-47 0.4453689930718596 1101 48-49 -1.2787116538190944 1101 50-51 -0.8212845371699018 1101 52-53 -0.11513689512221248 1101 54-55 -1.5558292396847406 1101 56-57 -0.8159296403016469 1101 58-59 -0.658788548423459 1101 60-61 -0.08704269295876799 1101 62-63 -1.6047165904585228 1101 64-65 -0.24859017394703642 1101 66-67 -0.9047491470076068 1101 68-69 0.1924676866142221 1101 70-71 0.08316328307944332 1101 72-73 -0.7739006307963692 1101 74-75 -1.91452075765973 1101 76 -2.0686112598054507 1102 1 0.5435957059976673 1102 2 -0.4598356957254559 1102 3 0.4865671485601517 1102 4 0.47191781035360236 1102 5 0.36340566772248195 1102 6 0.9658030121207375 1102 7 1.6303016642918493 1102 8 2.356854061084192 1102 9 1.2925096917900518 1102 10-11 -0.046410642518686984 1102 12-13 -3.918047862770006 1102 14-15 -0.7689820963261838 1102 16-17 -2.1211795144336705 1102 18-19 -0.16444060336680622 1102 20-21 0.1323153148559939 1102 22-23 -0.07245245570639014 1102 24-25 0.558942346743251 1102 26-27 -5.631189551139318 1102 28-29 -0.10586222754808716 1102 30-31 0.3566968757118616 1102 32-33 -6.6224887741812175 1102 34-35 -0.5812732445466651 1102 36-37 0.8825266681868484 1102 38-39 0.8131737227992346 1102 40-41 0.4911815538263724 1102 42-43 0.5328378740395152 1102 44-45 -6.021383984920952 1102 46-47 0.006718686323388567 1102 48-49 0.30547827291338336 1102 50-51 -6.588697421959779 1102 52-53 -1.55730160482846 1102 54-55 1.1268519992269432 1102 56-57 -0.7491627555930194 1102 58-59 1.1675295219729378 1102 60-61 0.715825709917759 1102 62-63 -4.537353814689617 1102 64-65 -0.5335615305472103 1102 66-67 0.6142042516028852 1102 68-69 -7.752920252008995 1102 70-71 -0.20157107139448271 1102 72-73 0.7915999854091567 1102 74-75 -6.669704332735229 1102 76 -1.9188665449528663 1103 1 -0.46574797656568734 1103 2 -0.15249971859318734 1103 3 0.0593224615791641 1103 4 0.03379287390646368 1103 5 0.24706721626557027 1103 6 -1.7443281609803023 1103 7 0.8395798167961175 1103 8 1.2953348935419058 1103 9 0.6524278921990501 1103 10-11 0.7445597672701467 1103 12-13 -1.1301279902146746 1103 14-15 -0.5730133247888318 1103 16-17 -0.4474005766985272 1103 18-19 -0.42919898951962665 1103 20-21 -0.7754172321166379 1103 22-23 -0.9948727745440209 1103 24-25 0.12443851097513914 1103 26-27 -0.9617604535334294 1103 28-29 0.47629890860756063 1103 30-31 -0.03918376919430244 1103 32-33 -5.7634518400841195 1103 34-35 -1.058608882730347 1103 36-37 -0.2077861813538746 1103 38-39 0.5381056506257806 1103 40-41 0.9650147233345621 1103 42-43 0.2808860951243801 1103 44-45 -4.707946933321679 1103 46-47 -0.06568073068504532 1103 48-49 0.4775907134561592 1103 50-51 -0.05086511112132541 1103 52-53 -0.4844672038070925 1103 54-55 0.15287760114313187 1103 56-57 0.9319939629327791 1103 58-59 0.7609606984634638 1103 60-61 0.8070763172006323 1103 62-63 0.25780825922376494 1103 64-65 0.6181179596741231 1103 66-67 0.11444151560993987 1103 68-69 -3.09019668717378 1103 70-71 0.31550628797010916 1103 72-73 1.3436398909554939 1103 74-75 -1.118410114068201 1103 76 -0.012325967158517415 1104 1 -1.4244766960067352 1104 2 0.11250386624085706 1104 3 0.05276783854906597 1104 4 0.2257853387258848 1104 5 -0.14034087843027976 1104 6 -0.31130481035541635 1104 7 1.4108477233936156 1104 8 1.5060563589658358 1104 9 0.012478782784292264 1104 10-11 -0.3543201478184912 1104 12-13 -0.5829680925581684 1104 14-15 -0.10834502737412777 1104 16-17 -0.5610767185736023 1104 18-19 1.0016523734202138 1104 20-21 0.9730363688843013 1104 22-23 0.44115023868472036 1104 24-25 0.6548129504054998 1104 26-27 0.6343956905909636 1104 28-29 -0.3089484449414286 1104 30-31 -1.0686180683241062 1104 32-33 -0.8772422046125357 1104 34-35 0.23194191647677087 1104 36-37 0.40642065654133575 1104 38-39 0.12298186841499614 1104 40-41 0.8535896463660606 1104 42-43 1.0169931059351143 1104 44-45 -2.902961905275628 1104 46-47 0.3900254816214783 1104 48-49 0.8458902368841095 1104 50-51 1.1927735657234564 1104 52-53 -0.045189723593978215 1104 54-55 -0.22655072326326575 1104 56-57 0.6602196106630842 1104 58-59 0.8943516703049497 1104 60-61 0.4626704155797299 1104 62-63 0.8328771442700926 1104 64-65 0.49123592180140463 1104 66-67 0.5591261205049989 1104 68-69 -3.4237549512706096 1104 70-71 -0.32911364933044496 1104 72-73 1.0882503389894538 1104 74-75 -0.9382282716040891 1104 76 0.8659724316156101 1105 1 -0.7252650244650347 1105 2 0.10169556203881669 1105 3 -0.7810589432159318 1105 4 -0.09981928423195541 1105 5 0.34416365178303465 1105 6 0.4803874205071068 1105 7 1.6545089918533407 1105 8 0.8394907600502322 1105 9 1.29417244048566 1105 10-11 0.7677762351450568 1105 12-13 -1.2336012522927788 1105 14-15 0.007546390190256602 1105 16-17 0.9901271766964186 1105 18-19 0.5693380408240856 1105 20-21 -0.09691083807866363 1105 22-23 0.4449509806409182 1105 24-25 0.19185903729999154 1105 26-27 0.2610222868669112 1105 28-29 -0.7715866699539049 1105 30-31 0.24346368954135045 1105 32-33 0.8437287592170932 1105 34-35 0.639914378487191 1105 36-37 0.6594794423014889 1105 38-39 0.21432809085916915 1105 40-41 0.26554655583314 1105 42-43 0.5012265367691029 1105 44-45 1.7687866857210182 1105 46-47 0.9986400211092459 1105 48-49 0.511571195719803 1105 50-51 0.15910373623069063 1105 52-53 -0.531643672370457 1105 54-55 -0.5224360767625811 1105 56-57 0.09763310855849738 1105 58-59 0.23013678837585871 1105 60-61 0.5601752311608053 1105 62-63 0.690045225885271 1105 64-65 0.37494142226512395 1105 66-67 0.5769223679188542 1105 68-69 1.53737082457301 1105 70-71 1.5526192464389794 1105 72-73 0.9073788971688046 1105 74-75 -0.3323479120406958 1105 76 -0.8765830367442398 1106 1 -0.3700835509072533 1106 2 -0.03182919117966421 1106 3 -0.825010450178361 1106 4 -0.45709886986420045 1106 5 0.40484750870604813 1106 6 -0.2405561669267371 1106 7 0.7342635240006246 1106 8 0.7824213609585371 1106 9 1.3672873299500594 1106 10-11 0.572229562473062 1106 12-13 0.298431551820876 1106 14-15 0.376403125542879 1106 16-17 0.6011638467410876 1106 18-19 -0.07584080540193838 1106 20-21 -0.28080341774312245 1106 22-23 0.2470340793910566 1106 24-25 0.8144859937238209 1106 26-27 0.006360649663889717 1106 28-29 -1.072859049190484 1106 30-31 -0.46357192957061244 1106 32-33 0.9571487944984156 1106 34-35 0.7705697100939872 1106 36-37 0.6216364816739315 1106 38-39 0.19926409656423516 1106 40-41 0.3800006338273576 1106 42-43 0.6219242810171295 1106 44-45 1.1687754257277803 1106 46-47 0.9554091537144345 1106 48-49 0.7264813034404014 1106 50-51 0.7603798687983456 1106 52-53 0.41292901088592515 1106 54-55 0.5775320199232254 1106 56-57 1.410598990778965 1106 58-59 -0.08808188069293976 1106 60-61 1.03244186780082 1106 62-63 0.9106960534947746 1106 64-65 1.067295886852449 1106 66-67 1.107219256407383 1106 68-69 1.318987008936631 1106 70-71 0.518567150203566 1106 72-73 0.2675898343755776 1106 74-75 -1.5683479871073196 1106 76 -0.9023834345973327 1107 1 -1.096806659558073 1107 2 -0.22545775950703018 1107 3 -0.0507566783342277 1107 4 0.1846464899534439 1107 5 -0.13927492698265098 1107 6 0.06678129101312891 1107 7 -0.21173669308392817 1107 8 0.7553617712483458 1107 9 -1.3619865222153607 1107 10-11 -0.5759469272696975 1107 12-13 -0.04672746261524452 1107 14-15 -0.510219038790126 1107 16-17 0.5226606447439082 1107 18-19 0.7521956648032031 1107 20-21 0.5034091080356689 1107 22-23 -0.13894225839250396 1107 24-25 -0.35412900681889425 1107 26-27 0.3147563948066221 1107 28-29 -1.0067133854544608 1107 30-31 -0.04111995952828096 1107 32-33 0.9450843203857389 1107 34-35 0.5101930683920415 1107 36-37 -0.10414773627896068 1107 38-39 0.40086509515317914 1107 40-41 0.5259574340010289 1107 42-43 0.35688032139485415 1107 44-45 0.00407337427873955 1107 46-47 0.2534771011799677 1107 48-49 0.5036819981109844 1107 50-51 0.8527384924822741 1107 52-53 1.0810447169534427 1107 54-55 0.6743897209977945 1107 56-57 0.6213175406541467 1107 58-59 0.7032781540405892 1107 60-61 -0.3314135538859304 1107 62-63 -0.4464623096876892 1107 64-65 0.09135970971145468 1107 66-67 1.0925660489830875 1107 68-69 1.4523746134085727 1107 70-71 0.06229340658042304 1107 72-73 0.2690443008902861 1107 74-75 -0.060418973756618755 1107 76 -0.6662605647395239 1108 1 -0.3680080848639733 1108 2 0.18960879958937937 1108 3 -0.1562158688680455 1108 4 0.023210427024849878 1108 5 0.30598189700551615 1108 6 0.9199815061536469 1108 7 1.738579294545488 1108 8 1.3955829625889393 1108 9 0.3458358523980607 1108 10-11 0.7731151945536183 1108 12-13 1.0583031276765311 1108 14-15 1.0330258852383807 1108 16-17 -0.49438251782164144 1108 18-19 0.2815102405729988 1108 20-21 0.876519905400194 1108 22-23 0.16467479149319786 1108 24-25 -0.27423456013340797 1108 26-27 -0.31371442676120864 1108 28-29 0.6702514525178458 1108 30-31 0.8097002636799928 1108 32-33 0.7889948353783467 1108 34-35 0.34145399349625194 1108 36-37 0.11098941579849253 1108 38-39 1.002297124197149 1108 40-41 0.6822469324547029 1108 42-43 0.7014026422231439 1108 44-45 0.5858654275260093 1108 46-47 1.0190630229226016 1108 48-49 0.23592954416449885 1108 50-51 0.705042849077671 1108 52-53 0.6116904746514393 1108 54-55 0.5551514528789312 1108 56-57 0.9808089350336004 1108 58-59 -0.8073544935323582 1108 60-61 1.3792207744722873 1108 62-63 0.6355432032879804 1108 64-65 0.5978996451692993 1108 66-67 0.14965392043663428 1108 68-69 1.5513005916255906 1108 70-71 2.0667861455880896 1108 72-73 1.024190865365206 1108 74-75 1.5235681850439704 1108 76 1.1897163076552602 1109 1 0.03432521168470615 1109 2 -0.579027225460969 1109 3 0.2771579275840743 1109 4 -0.1558940505870936 1109 5 0.4406654570242736 1109 6 0.9678820220584115 1109 7 -0.9030324250450974 1109 8 0.23023479584410467 1109 9 0.7750403382865443 1109 10-11 1.3475730807235138 1109 12-13 0.48038116172072876 1109 14-15 0.12389030160387193 1109 16-17 -0.6654794361872973 1109 18-19 0.043174999035770156 1109 20-21 1.0094722876663198 1109 22-23 1.0653424329531802 1109 24-25 0.8647665985831807 1109 26-27 0.5829201223049196 1109 28-29 0.20152128174508732 1109 30-31 0.7610548585430195 1109 32-33 1.1606893894104005 1109 34-35 0.27512953905864634 1109 36-37 0.6475683050858763 1109 38-39 1.0453972428276614 1109 40-41 0.45983197397538333 1109 42-43 0.9721440139228719 1109 44-45 0.4734544004065313 1109 46-47 -0.1544461640445931 1109 48-49 0.263949104403963 1109 50-51 1.183577279513301 1109 52-53 0.906364861497245 1109 54-55 0.6892487574834476 1109 56-57 0.40409051695762344 1109 58-59 -0.205301220158006 1109 60-61 0.5806012335447619 1109 62-63 -0.07849175132895425 1109 64-65 0.4165932611926948 1109 66-67 0.5471713011853865 1109 68-69 1.817958866793191 1109 70-71 0.465855378903953 1109 72-73 -0.20229339540120606 1109 74-75 1.7344287390208493 1109 76 0.07391362053632378 1110 1 0.289169430600527 1110 2 0.5539893682290753 1110 3 0.013155903449074913 1110 4 0.614067926599219 1110 5 0.4928115321000064 1110 6 0.8347564905472638 1110 7 1.183871843209019 1110 8 2.3801964919005556 1110 9 -0.9096760368250258 1110 10-11 -0.3924046397350125 1110 12-13 0.26897150631565836 1110 14-15 -0.05977886477580796 1110 16-17 0.5381780633031568 1110 18-19 1.4419260796286721 1110 20-21 0.17717679206950976 1110 22-23 0.6209059929006102 1110 24-25 0.9189227521644199 1110 26-27 -0.9280843546881101 1110 28-29 0.5384378494306219 1110 30-31 0.4648986065663294 1110 32-33 1.6422771405823653 1110 34-35 1.1794065784800836 1110 36-37 0.036695959127477806 1110 38-39 0.7539428877746985 1110 40-41 0.951549185448286 1110 42-43 1.1235658281944296 1110 44-45 2.131737540345945 1110 46-47 0.76761233907947 1110 48-49 1.2714482434437642 1110 50-51 0.6769549142272098 1110 52-53 0.3277785230028414 1110 54-55 -0.285330074843678 1110 56-57 0.919990167653765 1110 58-59 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46-47 -0.033840986138123696 2116 48-49 0.35288504731402526 2116 50-51 -0.061606621862836164 2116 52-53 0.8671847030934288 2116 54-55 -0.03947029286221948 2116 56-57 -0.29143367209707094 2116 58-59 -0.7477266969642571 2116 60-61 -1.3368189592913282 2116 62-63 0.6807033174779491 2116 64-65 0.9398004581522059 2116 66-67 -0.3526868962698586 2116 68-69 0.2604827215166807 2116 70-71 -3.3747128304258105 2116 72-73 0.4676582995042864 2116 74-75 -1.8274321407697869 2116 76 -1.839094937573897 2117 1 -0.5989950902885965 2117 2 0.009638527745316594 2117 3 0.8186071413375977 2117 4 0.08024557980304792 2117 5 -1.0493883258024148 2117 6 -1.4269568002608963 2117 7 -2.875184535176537 2117 8 -2.427737549838657 2117 9 0.09048401814018803 2117 10-11 -0.10462776416408559 2117 12-13 0.7692408671107742 2117 14-15 -0.7728159272596997 2117 16-17 0.03625740372054764 2117 18-19 -0.2535908198333843 2117 20-21 -0.15425409931952316 2117 22-23 -0.03770652573993516 2117 24-25 0.5966969731651943 2117 26-27 0.7842793058079778 2117 28-29 0.39040262172315465 2117 30-31 0.1897992553745027 2117 32-33 1.2578856227147668 2117 34-35 -0.4077472538603928 2117 36-37 -1.4143369809651247 2117 38-39 0.13657184913508758 2117 40-41 -1.577096749545973 2117 42-43 -1.3707053751846843 2117 44-45 0.6326432172223022 2117 46-47 -0.5053496927597578 2117 48-49 -0.43871954100513477 2117 50-51 0.684932287920418 2117 52-53 0.9689704250023539 2117 54-55 -0.7999965749490769 2117 56-57 -0.6874750301507504 2117 58-59 -0.8662170084730505 2117 60-61 0.821153181227011 2117 62-63 -0.5784803647036867 2117 64-65 -0.9356728670131105 2117 66-67 -1.508780677620308 2117 68-69 -0.05274220772467686 2117 70-71 0.9181511123354653 2117 72-73 1.2177724952036968 2117 74-75 -0.7492048849244597 2117 76 -0.925350044362883 2118 1 0.29457508363089957 2118 2 0.4199780377359943 2118 3 0.3717766984160171 2118 4 0.20755976467395243 2118 5 -0.21965048326181957 2118 6 0.4341426282539871 2118 7 0.7041430420729142 2118 8 -4.342076631009334 2118 9 -1.1869295375198234 2118 10-11 -0.37857365037834967 2118 12-13 0.5976283389694643 2118 14-15 -0.5864482800796083 2118 16-17 0.17847308368888548 2118 18-19 -0.7174070704621798 2118 20-21 -2.4655423735664144 2118 22-23 -0.7549739527374584 2118 24-25 -0.44354613479023897 2118 26-27 0.5344730994630922 2118 28-29 -0.316834952804939 2118 30-31 -0.7677887970404953 2118 32-33 -0.27678694847347884 2118 34-35 0.4974773406160722 2118 36-37 -0.0711346026634132 2118 38-39 -0.504551164479949 2118 40-41 -1.5637744665742446 2118 42-43 -0.25960182340054416 2118 44-45 0.10251036548681469 2118 46-47 -0.913064741867899 2118 48-49 -1.1249243190495477 2118 50-51 0.005359788477065308 2118 52-53 -1.392291872648208 2118 54-55 -0.03592179533782058 2118 56-57 0.6626070229074799 2118 58-59 0.331596834166497 2118 60-61 -1.9494858430425523 2118 62-63 0.39262376433815405 2118 64-65 0.1004284169729317 2118 66-67 -2.2642666873677157 2118 68-69 0.7085452418683573 2118 70-71 -0.5878585526076776 2118 72-73 -2.0808037399216133 2118 74-75 0.12442309723002865 2118 76 0.03335953729021668 2119 1 0.49896787603947246 2119 2 0.13469531392038192 2119 3 -0.14614454407607624 2119 4 -0.06076130722426143 2119 5 0.17520326802652164 2119 6 0.48113591111996357 2119 7 -2.3192069952444996 2119 8 0.15491132079789338 2119 9 -0.5385750576534534 2119 10-11 -0.3539486015103037 2119 12-13 -0.2761721728121742 2119 14-15 -1.0378778923339382 2119 16-17 -0.9493257221539579 2119 18-19 -1.2694159911004874 2119 20-21 -1.8136960502199315 2119 22-23 0.22797554436600365 2119 24-25 -0.8312118418663346 2119 26-27 0.14572785299047553 2119 28-29 0.48506290410944075 2119 30-31 -0.3366971739108138 2119 32-33 -0.23369866614060442 2119 34-35 0.23815687290452558 2119 36-37 -0.9694490995891769 2119 38-39 -0.6156859691854919 2119 40-41 -0.41955599978674485 2119 42-43 -1.483142001813313 2119 44-45 -2.0402820430855293 2119 46-47 -0.8698964936538047 2119 48-49 -1.3462920910223986 2119 50-51 0.4660965407960731 2119 52-53 -1.5817532580126752 2119 54-55 -1.717599834929814 2119 56-57 0.5097433558498707 2119 58-59 1.1148981811473462 2119 60-61 0.26880768505213126 2119 62-63 -0.5683928685651409 2119 64-65 -0.0707785347276797 2119 66-67 0.1484238979825392 2119 68-69 -0.9000350971869793 2119 70-71 -1.5751617476888882 2119 72-73 -3.004636238055742 2119 74-75 -0.7637535029761082 2119 76 0.6576531720060714 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 6.0 12 17.0 13 134.0 14 383.0 15 687.0 16 879.0 17 1025.0 18 1060.0 19 1104.0 20 1108.0 21 1139.0 22 1242.0 23 1373.0 24 1721.0 25 2009.0 26 2357.0 27 2870.0 28 3715.0 29 4405.0 30 5606.0 31 6963.0 32 9057.0 33 11826.0 34 16268.0 35 24104.0 36 40482.0 37 48654.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 36.530874853714465 23.4311041447128 12.370064021435796 27.66795698013694 2 17.819699885380192 20.95912594508765 37.71359769498512 23.507576474547044 3 16.878555580091906 27.247442085449592 29.560869428057668 26.31313290640083 4 12.554549565180814 18.858113294846316 39.66108289430792 28.92625424566495 5 12.10606012807975 37.49750255002787 36.90705279872131 13.489384523171077 6 26.21376068645699 39.79725964015689 19.14571437584782 14.843265297538302 7 25.350431664510975 31.161866304930758 24.763136586853424 18.72456544370485 8 22.25149058329916 35.815009937221994 20.973847755449697 20.95965172402915 9 23.364564602458543 15.738666834915929 23.56541215811224 37.331356404513286 10-11 24.96004080044586 25.912752242447183 27.835788720990145 21.291418236116808 12-13 23.58696909471382 23.930039854043766 27.023986035311314 25.4590050159311 14-15 21.551941701630966 25.668790813590338 24.376951954320326 28.40231553045837 16-17 22.147649242352546 27.718014238093737 26.63569828701221 23.49863823254151 18-19 21.860836829763294 27.033975835199847 27.254802990630616 23.85038434440624 20-21 21.271438636339738 27.80792243709055 26.97140814116113 23.94923078540858 22-23 22.459173265192383 27.01899113536705 27.6078635498491 22.913972049591468 24-25 22.012786943857325 27.724060695921004 27.03897073514411 23.224181625077552 26-27 20.82873276759519 28.190163727562386 27.680421043776356 23.300682461066067 28-29 21.848481024638 27.2066942174832 28.87025878839501 22.07456596948379 30-31 21.68207198965267 27.320262468847602 27.944624961881026 23.053040579618706 32-33 20.82873276759519 28.22092179564024 27.623636918094157 23.32670851867041 34-35 22.259114377950933 27.326571816145616 27.769803463831664 22.64451034207178 36-37 22.711547157113262 27.121255139489154 26.803684658822046 23.363513044575537 38-39 21.6760255318254 27.881005709959304 27.89914508344112 22.543823674774178 40-41 21.936023218398056 26.416711357876693 28.234066269177788 23.413199154547463 42-43 21.47281197093494 26.622553813474664 28.457785208786817 23.44684900680358 44-45 20.866325961912572 26.396994647570377 28.424398246001452 24.312281144515598 46-47 21.459141718455893 25.944036089466543 28.997760181709204 23.59906201036836 48-49 20.40968695121823 26.265024133253416 29.81850110939357 23.50678780613479 50-51 20.447805924477112 26.12963605581669 29.090297275413523 24.332260744292668 52-53 19.554507502865494 26.488480183391694 29.612395764324845 24.344616549417964 54-55 19.21432852771381 26.910154894476168 29.457028087111052 24.41848849069897 56-57 19.30187072147386 26.861783231858 29.29219638895023 24.54414965771791 58-59 19.921764093504528 26.641744744839478 28.612889996529862 24.823601165126135 60-61 19.88785135177766 26.474809930912645 28.427552919650463 25.209785797659233 62-63 19.51559986119436 26.26975614372693 28.725669579482005 25.488974415596704 64-65 20.175715322249914 26.342313637654186 27.956980767006318 25.524990273089582 66-67 20.648653480130815 26.103872887683107 27.477733261827396 25.769740370358683 68-69 20.136807680578777 26.08967685626255 27.365479457816754 26.408036005341913 70-71 21.076900427984057 26.12832160846294 26.855473884559977 25.939304078993032 72-73 21.25566526809468 25.74345142328359 26.316813358991347 26.68406994963038 74-75 20.995141802580523 26.069960145956234 26.373860374144293 26.56103767731895 76 21.691536010599705 25.5586401253457 26.243204307181088 26.506619556873506 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.5 8 1.0 9 0.5 10 0.5 11 1.0 12 1.0 13 1.0 14 1.0 15 2.5 16 6.0 17 8.0 18 8.0 19 11.5 20 17.5 21 20.0 22 32.5 23 82.0 24 124.5 25 130.0 26 258.0 27 508.0 28 759.0 29 888.0 30 1090.5 31 1564.0 32 2296.5 33 2758.0 34 3211.0 35 4254.5 36 5365.5 37 5886.0 38 6845.0 39 8900.5 40 10844.5 41 12270.5 42 12849.0 43 13034.5 44 13982.5 45 14863.0 46 14981.0 47 15053.5 48 14545.5 49 13076.5 50 12188.0 51 11309.0 52 9246.0 53 7201.0 54 6340.0 55 5860.5 56 4512.0 57 3166.0 58 2689.0 59 2097.5 60 1261.5 61 854.5 62 692.0 63 568.5 64 353.5 65 237.0 66 161.5 67 111.0 68 94.0 69 68.0 70 47.5 71 36.0 72 25.0 73 13.5 74 12.5 75 12.0 76 15.0 77 12.5 78 7.0 79 7.0 80 6.0 81 5.5 82 4.5 83 3.0 84 4.5 85 4.5 86 2.5 87 2.0 88 1.0 89 1.0 90 2.5 91 2.0 92 1.0 93 2.0 94 1.5 95 0.5 96 1.0 97 1.0 98 4.5 99 9.5 100 11.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.7103273499689791 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 190194.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 43.0087174148501 #Duplication Level Percentage of deduplicated Percentage of total 1 76.01100244498778 32.6913572457596 2 7.779951100244499 6.692114367435356 3 4.1283618581907096 5.32666645635509 4 2.4413202933985327 4.199922184716658 5 1.6772616136919316 3.606843538702588 6 1.1907090464547676 3.0726521341367237 7 0.9547677261613692 2.874433473190532 8 0.823960880195599 2.835000052577894 9 0.6259168704156479 2.4227893624404557 >10 4.2530562347188265 33.20662060843139 >50 0.11124694376528119 2.954351872298811 >100 0.0024449877750611247 0.11724870395490919 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.00525778941501835 0.0 2 0.0 0.0 0.0 0.00841246306402936 0.0 3 0.0 0.0 0.0 0.00946402094703303 0.0 4 0.0 0.0 0.0 0.012092915654542204 0.0 5 0.0 0.0 0.0 0.014196031420549544 0.0 6 0.0 0.0 0.0 0.014196031420549544 0.0 7 0.0 0.0 0.0 0.014721810362051379 0.0 8 0.0 0.0 0.0 0.01682492612805872 0.0 9 0.0 0.0 0.0 0.032072515431611937 0.0 10 0.0 0.0 0.0 0.043639652144652305 0.0 11 0.0 0.0 0.0 0.04942322050117249 0.0 12 0.0 0.0 0.0 0.052052115208681665 0.0 13 0.0 0.0 0.0 0.054155230974689 0.0 14 0.0 0.0 0.0 0.05678412568219818 0.0 15 0.0 0.0 0.0 0.05836146250670368 0.0 16 0.0 0.0 0.0 0.06256769403871835 0.0 17 0.0 0.0 0.0 0.06729970451223488 0.0 18 0.0 0.0 0.0 0.0720317149857514 0.0 19 0.0 0.0 0.0 0.07571216757626424 0.0 20 0.0 0.0 0.0 0.07939262016677708 0.0 21 0.0 0.0 0.0 0.08359885169879176 0.0 22 0.0 0.0 0.0 0.08938242005531194 0.0 23 0.0 0.0 0.0 0.09306287264582479 0.0 24 0.0 0.0 0.0 0.0977948831193413 0.0 25 0.0 0.0 0.0 0.10200111465135599 0.0 26 0.0 0.0 0.0 0.10568156724186883 0.0 27 0.0 0.0 0.0 0.11830026183791287 0.0 28 0.0 0.0 0.0 0.13302207219996426 0.0 29 0.0 0.0 0.0 0.15510478774304132 0.0 30 0.0 0.0 0.0 0.18402262952564225 0.0 31 0.0 0.0 0.0 0.20557956612721748 0.0 32 0.0 0.0 0.0 0.23712630261732756 0.0 33 0.0 0.0 0.0 0.2944362072410276 0.0 34 0.0 0.0 0.0 0.3412305330346909 0.0 35 0.0 0.0 0.0 0.42482938473348264 0.0 36 0.0 0.0 0.0 0.5794083935350222 0.0 37 0.0 0.0 0.0 0.8780508323080644 0.0 38 0.0 0.0 0.0 1.2823748383229754 0.0 39 0.0 0.0 0.0 1.7697719170951765 0.0 40 0.0 0.0 0.0 2.337613173917158 0.0 41 0.0 0.0 0.0 3.070023239429214 0.0 42 0.0 0.0 0.0 3.8523823043839447 0.0 43 0.0 0.0 0.0 4.614761769561605 0.0 44 0.0 0.0 0.0 5.351378066605676 0.0 45 0.0 0.0 0.0 6.25519206704733 0.0 46 0.0 0.0 0.0 7.126933552057373 0.0 47 0.0 0.0 0.0 8.070180973111665 0.0 48 0.0 0.0 0.0 9.00869638369244 0.0 49 0.0 0.0 0.0 9.821024848312776 0.0 50 0.0 0.0 0.0 10.691188996498312 0.0 51 0.0 0.0 0.0 11.559775807859344 0.0 52 0.0 0.0 0.0 12.324258388803011 0.0 53 0.0 0.0 0.0 13.086112075039171 0.0 54 0.0 0.0 0.0 13.861110234812875 0.0 55 0.0 0.0 0.0 14.57301492160636 0.0 56 0.0 0.0 0.0 15.238651061547683 0.0 57 0.0 0.0 0.0 15.90533875937201 0.0 58 0.0 0.0 0.0 16.591480278031906 0.0 59 0.0 0.0 0.0 17.251332849616706 0.0 60 0.0 0.0 0.0 17.89383471613195 0.0 61 0.0 0.0 0.0 18.58365668738236 0.0 62 0.0 0.0 0.0 19.327633889607455 0.0 63 0.0 0.0 0.0 19.98538334542625 0.0 64 0.0 0.0 0.0 20.587926012387353 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCTTACG 20 7.916351E-5 69.991585 8 TAAACCG 15 0.0022231021 69.991585 7 CCCGAAC 15 0.0022231021 69.991585 40 CAGGGCA 20 0.0069464645 52.49369 19 AGCGCAG 20 0.0069464645 52.49369 62 GGTATTG 20 0.0069464645 52.49369 33 GGTATGA 20 0.0069464645 52.49369 56 ACTCGTC 20 0.0069464645 52.49369 15 GGGCACG 20 0.0069464645 52.49369 21 CGCCGGC 20 0.0069464645 52.49369 18 AACAGTG 20 0.0069464645 52.49369 6 TGTAAAC 20 0.0069464645 52.49369 5 CCCGCGT 20 0.0069464645 52.49369 13 GGTTGCG 30 5.8704434E-4 46.661057 51 ACAAATG 55 1.3061253E-7 44.5401 24 AGCATGA 40 4.5835346E-5 43.74474 5 TAGTGTC 40 4.5835346E-5 43.74474 56 TGTGACG 50 3.4683962E-6 41.994953 38 ATAACGT 50 3.4683962E-6 41.994953 45 TCCGGCG 35 0.0012538692 39.99519 22 >>END_MODULE