FastQCFastQC Report
Wed 25 May 2016
SRR1780560_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780560_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences190194
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGT8990.4726752684101497No Hit
ATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGTCTT5440.2860237441769982RNA PCR Primer, Index 15 (95% over 21bp)
ATAAAGAATATTGAGGCGCCATTGGCGTGAAGGTAGCGGATGATTCAGCC3270.17192971387110004No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTGCC150.002222042370.042
TCTGTGA150.002222042370.08
GAGCCAG150.002222042370.018
GACTGTG150.002222042370.069
GGAGGCG252.385948E-456.00000416
CGTGCCC200.006943163552.537
GGCGTCC200.006943163552.519
CGTCTGC200.006943163552.541
TCAGATC200.006943163552.520
CCGGTCT200.006943163552.59
CGCAACC200.006943163552.537
GCGTCCT200.006943163552.520
TTCTGAT602.5845657E-740.83333219
TACCCGC350.001253128240.011
TACGGAC350.001253128240.038
CGGATGC350.001253128240.09
GAAAATT350.001253128240.070
TGCTCGT350.001253128240.025
CGTGTGT350.001253128240.029
GCTCGTG350.001253128240.026
Produced by FastQC (version 0.11.5)