##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780560_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 190194 Sequences flagged as poor quality 0 Sequence length 76 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.93216925875685 34.0 34.0 34.0 31.0 34.0 2 33.37005899239723 34.0 34.0 34.0 33.0 34.0 3 33.49986329747521 34.0 34.0 34.0 34.0 34.0 4 33.51705626886232 34.0 34.0 34.0 34.0 34.0 5 33.52459068109404 34.0 34.0 34.0 34.0 34.0 6 37.28079224370906 38.0 38.0 38.0 36.0 38.0 7 36.885648338012764 38.0 37.0 38.0 35.0 38.0 8 36.88831929503559 38.0 37.0 38.0 35.0 38.0 9 36.67915391652733 38.0 37.0 38.0 34.0 38.0 10-11 36.38708897231248 38.0 37.0 38.0 34.0 38.0 12-13 36.270996982028876 38.0 37.0 38.0 34.0 38.0 14-15 36.520815588294056 38.0 37.0 38.0 34.0 38.0 16-17 36.666180321145774 38.0 37.0 38.0 34.0 38.0 18-19 36.67831529911564 38.0 37.0 38.0 34.0 38.0 20-21 36.40681619819763 38.0 37.0 38.0 34.0 38.0 22-23 36.478845284288674 38.0 37.0 38.0 34.0 38.0 24-25 35.80523570669948 38.0 36.5 38.0 32.0 38.0 26-27 36.02222467585729 38.0 37.0 38.0 33.0 38.0 28-29 35.8621355037488 38.0 37.0 38.0 31.5 38.0 30-31 35.60017666172435 38.0 36.0 38.0 31.5 38.0 32-33 36.12575580722841 38.0 37.0 38.0 34.0 38.0 34-35 36.21142885685143 38.0 37.0 38.0 33.5 38.0 36-37 36.333738183118285 38.0 37.0 38.0 34.0 38.0 38-39 36.041320967012624 38.0 36.5 38.0 32.5 38.0 40-41 35.48903750906969 38.0 36.0 38.0 31.0 38.0 42-43 35.86313448373765 38.0 36.5 38.0 32.5 38.0 44-45 36.064439467070464 38.0 37.0 38.0 33.0 38.0 46-47 36.366354879754354 38.0 37.0 38.0 34.0 38.0 48-49 36.176606517555754 38.0 37.0 38.0 34.0 38.0 50-51 36.32772064313281 38.0 37.0 38.0 34.0 38.0 52-53 36.26351777658601 38.0 37.0 38.0 34.0 38.0 54-55 36.4223503370243 38.0 37.0 38.0 34.0 38.0 56-57 36.478495641292575 38.0 37.0 38.0 34.0 38.0 58-59 36.5719765081969 38.0 37.0 38.0 34.0 38.0 60-61 36.2303595276402 38.0 37.0 38.0 33.5 38.0 62-63 36.5043403051621 38.0 37.0 38.0 34.0 38.0 64-65 36.5101869669916 38.0 37.0 38.0 34.0 38.0 66-67 35.94759298400581 38.0 37.0 38.0 33.0 38.0 68-69 35.43920943878356 38.0 36.0 38.0 31.0 38.0 70-71 35.51400675100161 38.0 36.0 38.0 31.0 38.0 72-73 35.33414303290325 38.0 36.0 38.0 29.0 38.0 74-75 36.00254739897157 38.0 37.0 38.0 33.0 38.0 76 35.41269440676362 38.0 36.0 38.0 31.0 38.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.3411117467582514 1101 2 0.4123094810125352 1101 3 0.07401178068148084 1101 4 0.29451766027226256 1101 5 0.10205476596834018 1101 6 -1.105826635955502 1101 7 0.1302887599423812 1101 8 0.5899868398327399 1101 9 0.2430031413981908 1101 10-11 0.257979678308196 1101 12-13 0.5997337858116438 1101 14-15 0.5917486228958708 1101 16-17 -0.07029111226019324 1101 18-19 -0.47375306220414615 1101 20-21 -0.30374462284954973 1101 22-23 0.47062537907012825 1101 24-25 -1.5570417137865746 1101 26-27 -0.5371625370023665 1101 28-29 0.5166782049577563 1101 30-31 1.0687339175569122 1101 32-33 0.2684752149331544 1101 34-35 0.32492986229837584 1101 36-37 0.20173993261366974 1101 38-39 0.3385774881160657 1101 40-41 1.104337089060067 1101 42-43 0.3437111170819307 1101 44-45 0.17990804404936256 1101 46-47 0.5103774787599349 1101 48-49 -1.3430677518632663 1101 50-51 -1.6155930194236277 1101 52-53 -1.6446682973539453 1101 54-55 -1.0347972853673397 1101 56-57 -1.0963359509723887 1101 58-59 -1.3025436608444068 1101 60-61 -1.662002229268147 1101 62-63 -0.8271761001789883 1101 64-65 -1.402004713281194 1101 66-67 -1.4762910126299218 1101 68-69 -1.0705005727403574 1101 70-71 -0.8766239740178747 1101 72-73 -1.9074609495167323 1101 74-75 -1.0180655850164015 1101 76 -0.8047794411912434 1102 1 -0.7129924529194298 1102 2 0.08042524639397186 1102 3 0.23846417034005896 1102 4 0.3300138072948329 1102 5 0.11497476759349468 1102 6 0.33311484483378706 1102 7 -0.11517772795696146 1102 8 0.3188699503840766 1102 9 0.36019053559367364 1102 10-11 0.41721124970001 1102 12-13 0.3656772574187954 1102 14-15 -0.4241372910520411 1102 16-17 0.10865226454610166 1102 18-19 -0.48464838852221703 1102 20-21 0.6158236269913218 1102 22-23 0.41018983687697386 1102 24-25 -4.890672992650053 1102 26-27 -0.980471628403933 1102 28-29 -0.0972927318100858 1102 30-31 1.4669381185888852 1102 32-33 0.3245634609819916 1102 34-35 0.5044149582503792 1102 36-37 -2.6722020221798672 1102 38-39 -0.27722852225785033 1102 40-41 1.0796550104836982 1102 42-43 0.898682067392734 1102 44-45 0.6368752294121123 1102 46-47 0.9658210790253747 1102 48-49 -3.2636381821236995 1102 50-51 -0.6258992261707448 1102 52-53 -0.10777045204184788 1102 54-55 0.34559789874945324 1102 56-57 0.5726668795101517 1102 58-59 3.179705428024704E-4 1102 60-61 -5.530154560691919 1102 62-63 -2.399366947834025 1102 64-65 -0.07304576204946045 1102 66-67 0.8507786930811605 1102 68-69 -1.0146696432857283 1102 70-71 -1.62823897422102 1102 72-73 -6.344580899678569 1102 74-75 -2.089037564593589 1102 76 -0.5993324551811412 1103 1 0.6640539491413762 1103 2 0.41649276495140697 1103 3 0.12105238170839527 1103 4 0.35325438169969914 1103 5 0.24900633500472225 1103 6 0.2755839722671567 1103 7 0.6724935823052718 1103 8 0.6029879496835804 1103 9 0.47460827842208175 1103 10-11 0.26241908167190076 1103 12-13 -0.2932387850175715 1103 14-15 -0.343648301231795 1103 16-17 0.048969068486947265 1103 18-19 0.5565056184149384 1103 20-21 -0.21871297380634047 1103 22-23 0.6665140524934685 1103 24-25 -2.975760384892645 1103 26-27 -0.4785904637766052 1103 28-29 -0.02766704402691289 1103 30-31 1.009442270709009 1103 32-33 1.1530385313138183 1103 34-35 0.24375220079069493 1103 36-37 -1.060495868003457 1103 38-39 -0.25087527004621535 1103 40-41 -0.14598306022097773 1103 42-43 -0.2977216881575444 1103 44-45 1.0061371123844651 1103 46-47 0.8925686414201621 1103 48-49 -1.9515343282783277 1103 50-51 -0.3119085585550181 1103 52-53 0.18379876690764263 1103 54-55 -0.42884311854822244 1103 56-57 0.17457741968120644 1103 58-59 -0.27446199533637383 1103 60-61 -3.9356048538824453 1103 62-63 -0.8465192148351122 1103 64-65 0.21521870823039535 1103 66-67 -0.21829337868555854 1103 68-69 0.4552882141072985 1103 70-71 0.5117750356536845 1103 72-73 -4.8183801743286025 1103 74-75 -2.2473717452983806 1103 76 -3.4380042286731047 1104 1 0.28643385720093306 1104 2 0.2752924781646797 1104 3 0.34387090475676985 1104 4 -0.16819487532587374 1104 5 -0.35158340534082555 1104 6 0.021008037047216988 1104 7 0.46418660804578593 1104 8 0.5237279718954966 1104 9 0.925341412129363 1104 10-11 -0.5427592462490409 1104 12-13 0.335330233581395 1104 14-15 0.593840276502867 1104 16-17 0.2543343701050347 1104 18-19 0.5902961938159379 1104 20-21 -0.07577123087408921 1104 22-23 0.4579153711500652 1104 24-25 0.7405439323721907 1104 26-27 0.9274394379567639 1104 28-29 1.3752735742515227 1104 30-31 0.9086539586738098 1104 32-33 0.4635466639667385 1104 34-35 0.3250646717957153 1104 36-37 1.0086000509090027 1104 38-39 1.230028459587004 1104 40-41 1.569008575166258 1104 42-43 0.5029316654195597 1104 44-45 0.6592042542173715 1104 46-47 0.755237866674257 1104 48-49 0.07490909460557305 1104 50-51 0.361674729089259 1104 52-53 0.4558700980760193 1104 54-55 0.23221609447526959 1104 56-57 0.24169465078351493 1104 58-59 -0.4076087064268634 1104 60-61 0.3007903494690325 1104 62-63 0.2893477526764485 1104 64-65 0.4783560706360319 1104 66-67 0.10359835932633388 1104 68-69 1.287212215625587 1104 70-71 1.1101857592278321 1104 72-73 1.2126690573922616 1104 74-75 0.17290259994222623 1104 76 -0.13202107036401856 1105 1 0.7943064404926474 1105 2 0.30494218922981986 1105 3 0.2798379447796009 1105 4 -0.2167323655163571 1105 5 -0.2548472991840214 1105 6 0.1624327356275117 1105 7 0.583834178852662 1105 8 -0.4109085409103841 1105 9 0.452815193965705 1105 10-11 0.21944736001479015 1105 12-13 -0.5927998941206809 1105 14-15 0.14527960018126151 1105 16-17 0.13444780379270327 1105 18-19 0.5116113825153761 1105 20-21 0.21239492450866493 1105 22-23 0.1592887035321695 1105 24-25 0.45687588642798005 1105 26-27 0.3672607263922387 1105 28-29 0.6472593772050743 1105 30-31 1.1046213554063513 1105 32-33 0.822162987003054 1105 34-35 -0.7821836695041355 1105 36-37 0.44694634469512096 1105 38-39 0.1216725346387264 1105 40-41 0.6334802880862824 1105 42-43 0.2662565566230981 1105 44-45 0.7799843783523457 1105 46-47 0.9348519067684364 1105 48-49 -0.06399366632757619 1105 50-51 0.7390664676923819 1105 52-53 0.4789313808041271 1105 54-55 0.7411677246386503 1105 56-57 0.4398361931962924 1105 58-59 0.2623652537643295 1105 60-61 0.8583418940220682 1105 62-63 -0.37991278809548135 1105 64-65 0.32294835143791545 1105 66-67 0.45454185650833523 1105 68-69 0.23924546097052257 1105 70-71 1.1313065411355154 1105 72-73 1.217739685914765 1105 74-75 0.04606554493759063 1105 76 0.6642762828499329 1106 1 -0.8894528959184171 1106 2 0.1605740625173624 1106 3 0.30347267059876515 1106 4 0.19601770016694076 1106 5 0.10887675591487778 1106 6 0.07299460929038304 1106 7 -0.6413806993517426 1106 8 0.4625366416891694 1106 9 -1.0885615278749299 1106 10-11 0.38188589921832516 1106 12-13 1.1897095830402975 1106 14-15 -0.2585731941737279 1106 16-17 -0.05195950769624602 1106 18-19 0.5126229052417912 1106 20-21 0.21751259143806578 1106 22-23 0.2797293446011224 1106 24-25 -0.665841149941798 1106 26-27 0.7031031615534431 1106 28-29 0.5416988172080792 1106 30-31 0.6674333510524804 1106 32-33 0.23666585830132902 1106 34-35 0.6943279845888668 1106 36-37 0.9879908967350559 1106 38-39 0.015211175182209047 1106 40-41 1.1149557225343543 1106 42-43 0.9237331921371137 1106 44-45 0.9887859397380865 1106 46-47 0.9336001708528059 1106 48-49 1.198701976055034 1106 50-51 0.3762751153671928 1106 52-53 0.729184730652122 1106 54-55 0.07366969513746113 1106 56-57 0.514212201904023 1106 58-59 0.7132679298893905 1106 60-61 0.9021883077142192 1106 62-63 0.6120776033769602 1106 64-65 -0.7904060005494671 1106 66-67 0.2517137215385432 1106 68-69 1.5514907036233723 1106 70-71 0.3863284981223387 1106 72-73 0.5276503566350357 1106 74-75 -0.3273158310335873 1106 76 -0.013455203844507935 1107 1 0.800941112547882 1107 2 -0.25284176294665883 1107 3 0.1292948783486807 1107 4 0.28192891258417774 1107 5 0.1433172204758293 1107 6 0.20766301263348907 1107 7 0.8133028999101697 1107 8 0.004162605001880593 1107 9 -0.08036658594504331 1107 10-11 0.5898691837076413 1107 12-13 0.15792840427116772 1107 14-15 0.20974874222049777 1107 16-17 0.58573179806735 1107 18-19 0.6844833198733014 1107 20-21 0.8462165966480981 1107 22-23 0.11750205707262751 1107 24-25 0.2680403218914833 1107 26-27 -0.11425817568741081 1107 28-29 0.6653447522998022 1107 30-31 0.6024490896826791 1107 32-33 0.7810162327721883 1107 34-35 0.14927208531609892 1107 36-37 1.1053196712927473 1107 38-39 0.453683985341776 1107 40-41 0.4602884046140261 1107 42-43 0.9737331921371108 1107 44-45 0.7714300296110892 1107 46-47 0.887735631614774 1107 48-49 0.7462109307928984 1107 50-51 1.0085284530422172 1107 52-53 0.6845304378050585 1107 54-55 0.7992042658527581 1107 56-57 0.9856221660850721 1107 58-59 0.6727382435765747 1107 60-61 1.1051298034267987 1107 62-63 0.8558201410953927 1107 64-65 0.7423303502591665 1107 66-67 0.037652069525087484 1107 68-69 1.087944627426694 1107 70-71 0.23578578673625827 1107 72-73 1.1627257935176942 1107 74-75 -0.6806752145134496 1107 76 0.6960856623228651 1108 1 -0.3077754398253916 1108 2 0.2850196058848624 1108 3 -0.023851551663419457 1108 4 0.265894221176346 1108 5 0.10653155421255178 1108 6 -0.17608379387451123 1108 7 -0.027140155085128015 1108 8 -0.5012663313972041 1108 9 -0.0045463627367610115 1108 10-11 0.4337275943570873 1108 12-13 1.1792760578949384 1108 14-15 0.5936266499790008 1108 16-17 0.01075028670523892 1108 18-19 0.3731733158394164 1108 20-21 0.8194164756315558 1108 22-23 0.1599704583096866 1108 24-25 1.1831068809878929 1108 26-27 -0.18854552273595004 1108 28-29 -0.36952818033432777 1108 30-31 0.44191560844024025 1108 32-33 0.7784748854547132 1108 34-35 0.6069448700411257 1108 36-37 1.1470300383762506 1108 38-39 1.2501022319465775 1108 40-41 0.9867338799400969 1108 42-43 0.05806155034607485 1108 44-45 0.8381235888419596 1108 46-47 0.8130095996126201 1108 48-49 0.8210495754076703 1108 50-51 0.540330591001073 1108 52-53 -0.07371717547987089 1108 54-55 -0.11454959995334235 1108 56-57 0.312262612501641 1108 58-59 0.5714843777704672 1108 60-61 1.0307735441824804 1108 62-63 0.8456312208318479 1108 64-65 0.7104256843992829 1108 66-67 0.8791338498128383 1108 68-69 1.416207031621937 1108 70-71 -0.15167051284690558 1108 72-73 -0.2749406042233318 1108 74-75 -0.5277069477392118 1108 76 0.9906432796416738 1109 1 -1.3356294691243669 1109 2 0.13105858842134666 1109 3 0.22685195483366982 1109 4 0.18276320343694863 1109 5 0.06344178126582278 1109 6 -0.21773206821445967 1109 7 -0.21818700034128824 1109 8 0.3643397877298966 1109 9 0.6341927053240397 1109 10-11 0.36662893791655193 1109 12-13 0.5874150073252409 1109 14-15 0.6598528204206886 1109 16-17 0.6264788297570831 1109 18-19 0.4723935961384669 1109 20-21 0.9169814465257033 1109 22-23 0.2132490054997973 1109 24-25 -0.4552149111604251 1109 26-27 -0.19923261408144555 1109 28-29 -1.0458807060606006 1109 30-31 0.5838049372122782 1109 32-33 0.709407100534591 1109 34-35 0.024539774447006835 1109 36-37 0.9422682649881082 1109 38-39 0.9461270753819022 1109 40-41 0.5485700318974978 1109 42-43 0.9546943751315737 1109 44-45 0.5498100732406712 1109 46-47 0.5654529052655093 1109 48-49 0.3478345507159659 1109 50-51 0.07061068314662577 1109 52-53 -0.2591790757750658 1109 54-55 -0.7708228777418569 1109 56-57 0.13340987986169495 1109 58-59 -0.021133631995859048 1109 60-61 0.6846638661401627 1109 62-63 0.2630623185778731 1109 64-65 0.6364919795409492 1109 66-67 -0.03716267127856554 1109 68-69 -0.120997771534185 1109 70-71 0.19312871191651482 1109 72-73 -0.05390507024810631 1109 74-75 -0.44977980894152125 1109 76 0.46944497357496573 1110 1 -0.9596042236628541 1110 2 -0.5275548791046418 1110 3 -0.20875876464792498 1110 4 -0.15625832408215246 1110 5 0.22938922403832862 1110 6 -0.2957350622474806 1110 7 0.3620474619627103 1110 8 0.5261370509307 1110 9 0.8526481778681969 1110 10-11 -0.4444078690104831 1110 12-13 0.3150054432873688 1110 14-15 0.6712754780818315 1110 16-17 0.3515900517583006 1110 18-19 -0.07522277405333 1110 20-21 0.6947676191534882 1110 22-23 -0.8431614581536309 1110 24-25 -1.5618281397260247 1110 26-27 0.8619553001458371 1110 28-29 0.32023119768054187 1110 30-31 -0.19596320093492636 1110 32-33 0.6334774392257856 1110 34-35 0.642424553373516 1110 36-37 1.1081559676651622 1110 38-39 0.7251994171233562 1110 40-41 -1.083430980169993 1110 42-43 0.03685106285954731 1110 44-45 0.5452627977404276 1110 46-47 0.4211015069958606 1110 48-49 1.1271686946404031 1110 50-51 -0.39458016888769976 1110 52-53 0.3904839198041685 1110 54-55 -0.629592020928321 1110 56-57 0.5527923781222057 1110 58-59 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44-45 -0.1634279052468557 2116 46-47 -0.7665956227164799 2116 48-49 0.023965608547577233 2116 50-51 -0.7999954554816924 2116 52-53 0.05244450571912296 2116 54-55 -0.34743468078618633 2116 56-57 -0.2400188595559598 2116 58-59 -2.825252054413724 2116 60-61 -1.7869006886036871 2116 62-63 0.4545034397786978 2116 64-65 -1.7657084577796383 2116 66-67 -1.027559295686281 2116 68-69 -1.5403298008477293 2116 70-71 0.973277124227593 2116 72-73 0.08185261264451071 2116 74-75 0.14642111258287827 2116 76 -1.3739074076702025 2117 1 0.842278959635216 2117 2 0.43136564774945896 2117 3 0.3338900279130286 2117 4 0.13025567877541988 2117 5 0.18959799817343992 2117 6 0.3479630662805633 2117 7 0.6210983750567678 2117 8 0.38113506000308206 2117 9 -0.6560182129313716 2117 10-11 -0.3308644863073482 2117 12-13 -1.1625536794552644 2117 14-15 0.1636104067597799 2117 16-17 -0.7978114988229663 2117 18-19 -1.1511512880405732 2117 20-21 -0.1745670206447656 2117 22-23 -0.03278560224993754 2117 24-25 -1.6847484011231089 2117 26-27 -1.7779483545726578 2117 28-29 -0.8642184865036882 2117 30-31 -0.10873910056095326 2117 32-33 -1.4950701205215609 2117 34-35 0.4529311858951175 2117 36-37 -1.1408427128573138 2117 38-39 0.4384297166994102 2117 40-41 -0.29774848237563845 2117 42-43 0.35032456790712985 2117 44-45 -0.09538647888920337 2117 46-47 -0.5204686712507183 2117 48-49 -0.50935819068998 2117 50-51 -0.28952360312072756 2117 52-53 0.28702595179954216 2117 54-55 -0.08245583367182263 2117 56-57 0.31809714619865304 2117 58-59 -0.7941374616974883 2117 60-61 -0.7901622031588431 2117 62-63 -0.4667634281000801 2117 64-65 -0.7992627829150791 2117 66-67 -0.4538827918249737 2117 68-69 0.40481570795280675 2117 70-71 -0.33700219631866446 2117 72-73 0.9716996544422756 2117 74-75 0.9208472525120968 2117 76 0.8536600745173999 2118 1 0.07239449363981265 2118 2 0.224831090629813 2118 3 -0.9334751205658947 2118 4 0.06750364394203956 2118 5 0.19321410531247096 2118 6 0.08905886631849569 2118 7 0.7321132765518712 2118 8 0.6231792119702959 2118 9 -1.2070354234572562 2118 10-11 -0.5558651047977392 2118 12-13 -1.2108928265982541 2118 14-15 0.49343483795478704 2118 16-17 -2.4395097084994504 2118 18-19 -0.3699072152401328 2118 20-21 -0.41401351297960076 2118 22-23 0.6562353686975086 2118 24-25 0.6273315408212596 2118 26-27 -2.263764308326074 2118 28-29 -0.3729598173300701 2118 30-31 -0.9229280947306506 2118 32-33 -0.10670763567458152 2118 34-35 -0.23237884272036524 2118 36-37 -2.6515673361966705 2118 38-39 -0.22976874449650353 2118 40-41 -0.24950210879094925 2118 42-43 0.6295564853097986 2118 44-45 -1.8656317309446493 2118 46-47 0.1956483636238957 2118 48-49 0.20974615276185915 2118 50-51 -1.0514357280062967 2118 52-53 -1.4234257111149446 2118 54-55 -0.7124748831757941 2118 56-57 0.2863005553176805 2118 58-59 -0.49757544360458894 2118 60-61 -0.701225346904252 2118 62-63 -1.1961553283499526 2118 64-65 0.16742532475173277 2118 66-67 -0.012342503361054469 2118 68-69 -1.40533660561357 2118 70-71 -0.7150771243676175 2118 72-73 0.9764455373579253 2118 74-75 -0.09237607199744247 2118 76 0.1893560411354116 2119 1 0.5319584130342037 2119 2 -1.0571908022514478 2119 3 -0.06631621947480681 2119 4 -0.5200323407045104 2119 5 -0.17746329455247434 2119 6 -0.4218076084300577 2119 7 -0.43159465250951 2119 8 0.07988212983644871 2119 9 -0.4651030923629662 2119 10-11 -0.30245322719884626 2119 12-13 -0.12513386153445794 2119 14-15 -0.23649816840694626 2119 16-17 -0.2282620630504013 2119 18-19 -0.07282952394397313 2119 20-21 -1.147505001055805 2119 22-23 -0.3325916206759132 2119 24-25 0.812927908593224 2119 26-27 0.20953597244302102 2119 28-29 -0.07764137542083915 2119 30-31 1.1432347868843067 2119 32-33 0.6498095836451796 2119 34-35 0.5938108718902839 2119 36-37 -0.1201407448756342 2119 38-39 -1.6970893543696235 2119 40-41 -0.7880812039325775 2119 42-43 0.2578039885972885 2119 44-45 0.07091978485305361 2119 46-47 0.15392394382078578 2119 48-49 -0.5126745033137752 2119 50-51 0.5341144372565196 2119 52-53 -0.0460045219334404 2119 54-55 0.1961507698789049 2119 56-57 -1.4384968644498883 2119 58-59 -0.11283737629108259 2119 60-61 0.4708656720387765 2119 62-63 -0.37991692340273175 2119 64-65 -0.3265905618033358 2119 66-67 0.6442573473692974 2119 68-69 0.6979482150851481 2119 70-71 -0.11076607485985335 2119 72-73 0.7130068267633334 2119 74-75 -0.29466309620044484 2119 76 -0.04018157836915748 >>END_MODULE >>Per sequence quality scores pass #Quality Count 20 3.0 21 5.0 22 6.0 23 24.0 24 43.0 25 126.0 26 238.0 27 439.0 28 830.0 29 1445.0 30 2357.0 31 3951.0 32 6335.0 33 10054.0 34 15977.0 35 25290.0 36 45714.0 37 77357.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 36.95384712451497 23.919261385742978 12.006687908135904 27.12020358160615 2 17.390664269114694 20.006414503086322 38.462832686625234 24.14008854117375 3 16.917463221763043 26.019748257042806 30.066668769782435 26.99611975141172 4 12.810603909692208 18.859164852729318 40.30621365553067 28.024017582047804 5 12.112895254319273 37.87343449320168 37.161004027466696 12.852666225012355 6 26.975088593751646 40.72788836661514 18.100991619083672 14.196031420549543 7 26.290524411916255 30.311155977580782 25.0423252047909 18.35599440571206 8 22.399760244802675 36.95857913498848 20.889197345867903 19.752463274340936 9 23.648485230869536 14.952101538429183 23.511256927137556 37.88815630356373 10-11 24.878807953983827 26.183528397320632 27.74824652723009 21.18941712146545 12-13 23.805167355437078 23.6818721936549 27.173044365227085 25.339916085680937 14-15 21.704943373608003 25.516577810025552 24.2265791770508 28.551899639315646 16-17 22.34376478753273 28.222499132464744 26.35282921648422 23.0809068635183 18-19 22.1500152475893 27.54634741369339 27.323680031967363 22.979957306749952 20-21 21.20676782653501 28.146524075417734 26.824452927011365 23.822255171035888 22-23 21.929713871100034 27.692251069960143 27.842098068288166 22.53593699065165 24-25 22.073514411600787 28.0663427868387 27.21799846472549 22.64214433683502 26-27 21.22648453684133 28.432022040653226 27.784525274193715 22.556968148311725 28-29 21.631597211268495 27.920702020042693 28.531920039538576 21.915780729150235 30-31 21.94206967622533 27.597610860489812 28.09552351809205 22.364795945192803 32-33 21.474126418288694 28.711736437532203 27.481939493359413 22.33219765081969 34-35 22.000168249261282 27.416217125671682 28.670988569565814 21.912626055501224 36-37 22.304857145861597 27.645193854695734 27.13203360778994 22.917915391652734 38-39 21.564297506756258 27.940155840878262 27.739834064166065 22.755712588199415 40-41 21.514611396784336 26.514243351525284 28.829248030957867 23.141897220732517 42-43 21.861099719234044 26.606254666288105 28.57608547062473 22.956560143853117 44-45 20.590292017624108 26.58680084545254 28.998285960650705 23.824621176272647 46-47 21.356877714333784 26.018170920218303 29.020105786723033 23.60484557872488 48-49 20.664426848375868 25.89461286896537 29.742263162875798 23.698697119782956 50-51 20.20699916926927 26.450886989074313 29.423378234854937 23.918735606801476 52-53 19.51244518754535 26.394891531804372 30.62399444777438 23.468668832875906 54-55 18.749014164484684 27.022671587957557 29.947054060590766 24.28126018696699 56-57 19.17515799657192 26.636749844895213 29.583215033071497 24.60487712546137 58-59 19.195137596348992 26.923299368013716 29.312701767668802 24.568861267968494 60-61 19.44356814620861 26.872561700158787 28.416248672408173 25.267621481224435 62-63 18.365984205600597 27.369422799878016 28.301365973690025 25.963227020831365 64-65 19.20039538576401 27.855768320767215 26.97167103063188 25.972165262836892 66-67 19.124157439246243 27.487460172245182 26.74111696478333 26.647265423725248 68-69 19.218797648716574 27.989579061379434 26.484273951859684 26.30734933804431 70-71 19.77060264782275 27.808973994973552 25.827576053923888 26.59284730327981 72-73 19.624436102085237 27.818438015920588 25.499752883897493 27.057372998096678 74-75 19.536368129383682 28.469089456029106 25.30495178607106 26.689590628516147 76 19.91072273573299 28.29216484221374 25.48082484200343 26.316287580049845 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.5 12 1.0 13 0.5 14 0.0 15 1.0 16 1.0 17 0.0 18 3.0 19 5.5 20 7.5 21 10.0 22 24.0 23 74.5 24 102.5 25 94.0 26 254.0 27 488.0 28 743.0 29 924.0 30 1191.0 31 1686.5 32 2365.0 33 2815.0 34 3307.0 35 4506.0 36 5808.0 37 6403.0 38 7475.0 39 9441.0 40 11372.5 41 12853.0 42 13296.0 43 13526.0 44 14447.5 45 15506.0 46 15873.0 47 15630.5 48 14548.0 49 12796.5 50 11885.0 51 10799.0 52 8392.0 53 6497.0 54 5923.0 55 5547.5 56 4099.0 57 2643.5 58 2261.0 59 1700.0 60 931.0 61 586.0 62 449.0 63 361.0 64 209.0 65 117.0 66 68.5 67 48.0 68 40.5 69 24.5 70 10.0 71 4.0 72 2.5 73 0.5 74 0.5 75 1.0 76 0.5 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 190194.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 28.98882193970367 #Duplication Level Percentage of deduplicated Percentage of total 1 61.541670445270704 17.840205264098763 2 11.330370907771833 6.569082095123926 3 6.542123877754602 5.689453925991357 4 4.013784347510656 4.654195190174243 5 2.7623106919379707 4.0038066395364735 6 1.9570146005259816 3.4038928672828797 7 1.5307880656570236 3.106301986392841 8 1.235150086152172 2.864443673301997 9 1.0084338442005985 2.630997823275182 >10 7.7863426135848375 42.52868124125892 >50 0.26299084066382517 4.965982102484831 >100 0.02539221909857622 0.984258178491435 >500 0.0036274598712251744 0.7586990125871478 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGT 899 0.4726752684101497 No Hit ATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGTCTT 544 0.2860237441769982 RNA PCR Primer, Index 15 (95% over 21bp) ATAAAGAATATTGAGGCGCCATTGGCGTGAAGGTAGCGGATGATTCAGCC 327 0.17192971387110004 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.005783568356520184 0.0 2 0.0 0.0 0.0 0.008938242005531194 0.0 3 0.0 0.0 0.0 0.009989799888534864 0.0 4 0.0 0.0 0.0 0.013144473537545874 0.0 5 0.0 0.0 0.0 0.015247589303553214 0.0 6 0.0 0.0 0.0 0.015247589303553214 0.0 7 0.0 0.0 0.0 0.01577336824505505 0.0 8 0.0 0.0 0.0 0.01787648401106239 0.0 9 0.0 0.0 0.0 0.0331240733146156 0.0 10 0.0 0.0 0.0 0.04679432579366331 0.0 11 0.0 0.0 0.0 0.05310367309168533 0.0 12 0.0 0.0 0.0 0.055732567799194506 0.0 13 0.0 0.0 0.0 0.05730990462370001 0.0 14 0.0 0.0 0.0 0.058887241448205514 0.0 15 0.0 0.0 0.0 0.06099035721421286 0.0 16 0.0 0.0 0.0 0.06414503086322387 0.0 17 0.0 0.0 0.0 0.06940282027824221 0.0 18 0.0 0.0 0.0 0.0751863886347624 0.0 19 0.0 0.0 0.0 0.07886684122527525 0.0 20 0.0 0.0 0.0 0.08254729381578808 0.0 21 0.0 0.0 0.0 0.08675352534780277 0.0 22 0.0 0.0 0.0 0.09043397793831562 0.0 23 0.0 0.0 0.0 0.09569176735333397 0.0 24 0.0 0.0 0.0 0.0993722199438468 0.0 25 0.0 0.0 0.0 0.10357845147586149 0.0 26 0.0 0.0 0.0 0.10568156724186883 0.0 27 0.0 0.0 0.0 0.1156713671304037 0.0 28 0.0 0.0 0.0 0.13144473537545875 0.0 29 0.0 0.0 0.0 0.15510478774304132 0.0 30 0.0 0.0 0.0 0.18559996635014775 0.0 31 0.0 0.0 0.0 0.2061053450687193 0.0 32 0.0 0.0 0.0 0.24028097626633857 0.0 33 0.0 0.0 0.0 0.3023228913635551 0.0 34 0.0 0.0 0.0 0.35595234339674225 0.0 35 0.0 0.0 0.0 0.4400769740370359 0.0 36 0.0 0.0 0.0 0.6030684459026047 0.0 37 0.0 0.0 0.0 0.9153811371546947 0.0 38 0.0 0.0 0.0 1.334426953531657 0.0 39 0.0 0.0 0.0 1.8433809689054335 0.0 40 0.0 0.0 0.0 2.4511814252815545 0.0 41 0.0 0.0 0.0 3.2314373744702776 0.0 42 0.0 0.0 0.0 4.056384533686657 0.0 43 0.0 0.0 0.0 4.880280135020032 0.0 44 0.0 0.0 0.0 5.67630945245381 0.0 45 0.0 0.0 0.0 6.622185768215611 0.0 46 0.0 0.0 0.0 7.529680221247778 0.0 47 0.0 0.0 0.0 8.531289104808774 0.0 48 0.0 0.0 0.0 9.541836230375301 0.0 49 0.0 0.0 0.0 10.405691031262815 0.0 50 0.0 0.0 0.0 11.325278399949525 0.0 51 0.0 0.0 0.0 12.250649336992755 0.0 52 0.0 0.0 0.0 13.084008959273163 0.0 53 0.0 0.0 0.0 13.886347624004964 0.0 54 0.0 0.0 0.0 14.704985435923321 0.0 55 0.0 0.0 0.0 15.463684448510469 0.0 56 0.0 0.0 0.0 16.161393103883402 0.0 57 0.0 0.0 0.0 16.881710253740916 0.0 58 0.0 0.0 0.0 17.596769614183412 0.0 59 0.0 0.0 0.0 18.303942290503382 0.0 60 0.0 0.0 0.0 18.987454914455768 0.0 61 0.0 0.0 0.0 19.717761864201815 0.0 62 0.0 0.0 0.0 20.499595150215043 0.0 63 0.0 0.0 0.0 21.19835536347098 0.0 64 0.0 0.0 0.0 21.837702556337213 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTCTGCC 15 0.0022220423 70.0 42 TCTGTGA 15 0.0022220423 70.0 8 GAGCCAG 15 0.0022220423 70.0 18 GACTGTG 15 0.0022220423 70.0 69 GGAGGCG 25 2.385948E-4 56.000004 16 CGTGCCC 20 0.0069431635 52.5 37 GGCGTCC 20 0.0069431635 52.5 19 CGTCTGC 20 0.0069431635 52.5 41 TCAGATC 20 0.0069431635 52.5 20 CCGGTCT 20 0.0069431635 52.5 9 CGCAACC 20 0.0069431635 52.5 37 GCGTCCT 20 0.0069431635 52.5 20 TTCTGAT 60 2.5845657E-7 40.833332 19 TACCCGC 35 0.0012531282 40.0 11 TACGGAC 35 0.0012531282 40.0 38 CGGATGC 35 0.0012531282 40.0 9 GAAAATT 35 0.0012531282 40.0 70 TGCTCGT 35 0.0012531282 40.0 25 CGTGTGT 35 0.0012531282 40.0 29 GCTCGTG 35 0.0012531282 40.0 26 >>END_MODULE