FastQCFastQC Report
Wed 25 May 2016
SRR1780559_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780559_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40
Sequences flagged as poor quality0
Sequence length50
%GC61

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAGAACGGATGCCACGAACTCGGACTCCTCGGCCTGTCTCTTATACA12.5No Hit
GTACACGGGAGCGTCCGGCCCCCTGCGTCAGGCGATGCGCACCGGCATCA12.5No Hit
AGACAGCCCGAAGCGCCCGAGCTGAGGGAGTTGATCGTCATCTTCCTGTC12.5No Hit
GTCTAGTAAGGGCTTAGCTTAATTAAAGTGGCTGATTTGCGTTCAGTTGA12.5No Hit
ACTTCATGGTGGCCGTGCCGATCATGGCGGTCCCCGGCCTCCTCACGGCC12.5No Hit
CGCCAGGACCGCGTGATGACGCGCATGACCTGGGCGCTGTCTCTTATACA12.5No Hit
GCGCGGCTGGGTCATGGAGCGCGTGGAGGACGTCGCCGAGCCTGTCTCTT12.5No Hit
GCAGTAATGTTAGCGGTTAGGCGTACGGCCCGGGGTATTGGTTGGATGAG12.5No Hit
GCTCCGGGCCCATAACACTTGGGGGTAGCTAAAGTGAACCGTATCCGACA12.5No Hit
GCCCAGTCCGTGACCCAGGATCCGCGCCTGGCGGAATCGGTGGGCTCCGC12.5No Hit
GAAGGCTACAAAGTAAGCGCAAGTACCCACGTAAAGACGTTAGGTCAAGG12.5No Hit
CCCTTCCAGCAGCCCCCGGCGGCGCAGCATGCGCCGCTCGGCGGCTGTCT12.5No Hit
GGCCAACGCGCAGCAGCACCAGGACCGGGCGATGCTCGCCGAGGACGCGG12.5No Hit
ATCCTACACTCCAACTCATGAGACCCACAACAAATAGCCCTTCTAAACGC12.5No Hit
TCTCTTATACACATCTGACGCTGCCGACGATCCGTGCCCTGCCGCTTTCC12.5No Hit
GCGTGGTGATTGGTGCCGTGGTGGAGTGAACTGGGTATCGGTGGGGGGGT12.5No Hit
GGGGTTGGTGGGGCTGCGCGTAGCCGGTGCCTGCTTCGAAGCAGGGAGCC12.5No Hit
CGGTGCGCCTGCGCGGCCTGGAGGCGCACCAGGAGGTGCTGCTGGGCGGC12.5No Hit
AGCTTGGCGATGTCGGTGGTGAAGGCCTGCGCGGTGAGCTCTGTCTCTTA12.5No Hit
ATGATGGCCAGGCTGGTCTCGAACTCCTGGCCTCCCAAAGTGCTGGAATT12.5No Hit
CATAAATAGTATGGCTTTGAAGAAGGCGTGGGTACAGATGTGCAGGAATG12.5No Hit
CCGCGGCACCGGCTTCATCCGCGTGGTGTACCTCATGGTCTGTCTCTTAT12.5No Hit
ACATAACTGGAATACATACCAAAGAAGCATCTCTGCAAATATAAACATTT12.5No Hit
GAAATAGGGTCGACGCCGCACTCGTCCGGGGGGGATTTTATTCTGACACC12.5No Hit
GTGTTAGTCATGTTAGCTTGTTTCAGGTGCGAGATAGTAGTAGGGTCGTG12.5No Hit
GTGTGCGCCTCGACGGCGGCACCATCTACGCCGGTGCGGAGATCAGCCCC12.5No Hit
GGTTGTAATCCAGGGAGAATGCGTGCCCACCACGAGCACCGATACCGTGA12.5No Hit
CAGCCTGAGTCCACCGCCCACGCCCGCCACGGGGTCCCCGCCGGGGCGAC12.5No Hit
CTCTTACCGCGAGGATGAGCGGCTGCGTGAGGGAGGCAGGTGGCCCCACG12.5No Hit
GTGTAGGAAGAGGCAGATAAAGAATATTGAGGCGCCATTGGCGTGAAGGT12.5No Hit
GTTGGAGTGTAGGATAAATCATGCTAAGGCGAGGATGAAACCGATATCGC12.5No Hit
GCCATTCTCATCCAAACCCCCTGAAGCTTCACCGGCGCAGTCATTCTCAT12.5No Hit
ATCTTATCCACCTCTGACCCCGGCGACGCGGAGACGGTTATGGAGCTGGC12.5No Hit
GTTCGGCGCGCTGGACGTGGTGGACGAGCACGGCGCCCTGCGCCGCGCCC12.5No Hit
CAGCTCGAGTGCGCGGCCGGCGTCCACGTCCTCGCGCACGTCCGCGCCGA12.5No Hit
GTCCACCGCGAGCACGGGCGAGAAGAACACGACCCAGCCCAGGGCGGTCA12.5No Hit
NNTGAGAGGGCCCCTGTTAGGGGGCATGGGCTGGGGTTTATTATATGAGA12.5No Hit
ACCCGGTGCCGGGCGACCTCCACCGGCCCCTGCGCACCGGCGTCTGTCTC12.5No Hit
GCACGTGCACGTCCGCCGCACGCCAGGTGCGCTCCGGGACTGTCTCTTAT12.5No Hit
GCCCCACCCGCACTCTCGAACGTGATGTGGGGCCTATCTCTGTCTCTTAT12.5No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers