FastQCFastQC Report
Wed 25 May 2016
SRR1780559_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780559_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40
Sequences flagged as poor quality0
Sequence length50
%GC61

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACAGTGCAAGGATGTGATAGTGATTTTGCATATACCAAAGGCACTCAA12.5No Hit
CTCCACCGTGGTCCGATTCCCGGGCCGCACCGCGCGCCGGCGCCGCGCCA12.5No Hit
GCCGCGGCGTCCCGGTAGCGGGCGGCGGCGTCGGCGGACTGCCGGGCCAC12.5No Hit
GGACATCGCGGACTCGCTGCCCGAGGGCGGCGCGCAGTCGCCCGCGATGA12.5No Hit
AGCTCACCGCGCAGGCCTTCACCACCGACATCGCCAAGCTCTGTCTCTTA12.5No Hit
GACTAACACCCTTAATTCCATCCACCCTCCTCTCCCTAGGAGGCCTGCCC12.5No Hit
CCGCCGAGCGGCGCATGCTGCGCCGCCGGGGGCTGCTGGAAGGGCTGTCT12.5No Hit
GAATTATATCCTTCTTGCTCATCAGTTGATGATACGCCCGAGCAGATGCC12.5No Hit
CCGCCCAGCAGCACCTCCTGGTGCGCCTCCAGGCCGCGCAGGCGCACCGC12.5No Hit
GAAGATGACGATCAACGCCCTCAACTCGGGCGCCTCGGGCTGGCTCTGTC12.5No Hit
CTGCTGGATCGCGTCGCGGTGGTTGCGCAGCAGCAGACCCGCGAACTGGA12.5No Hit
TCTCTTATACACATCTCCGAGCCCACGACAAACGGTCATCTCGTATGCCG12.5No Hit
AGATAGGCCCCACATCACGTTCGAGAGTGCGGGTGGGGCCTGTCTCTTAT12.5No Hit
CCTTTTCATCAATCGCCCACATCACTCGAGACGTAAATTATGGCTGAATC12.5No Hit
GCTCGGCGACGTCCTCCACGCGCTCCATGACCCAGCCGCGCCTGTCTCTT12.5No Hit
CTTAAAGACCTTTCTTTCTGCTACGGCCTGGCATAGTGGCTCCCACCTGT12.5No Hit
TCCCGGAGCGCACCTGGCGTGCGGCGGACGTGCACGTGCCTGTCTCTTAT12.5No Hit
ATAGTGGGGGGTAAGGCGAGGTTAGCGAGGCTTGCTAGAAGTCATCAAAA12.5No Hit
GCACCCTCCTGCGTGACGGCGTGCGGCAGGTCGTGGCGGCCCGCCGCGAC12.5No Hit
CTTATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCGT12.5No Hit
GGCTTGGATTAGCGTTTAGAAGGGCTATTTGTTGTGGGTCTCATGAGTTG12.5No Hit
AACTAAAGAAGCAGCCAGGGAAAATCAGACATCTCCGAGCCCACGAGGCT12.5No Hit
ACCATGAGGTACACCACGCGGATGAAGCCGGTGCCGCGGCTGTCTCTTAT12.5No Hit
CGCCAGGGCCGTTCCGCGTCCTCGGCGAGCATCGCCCGGTCCTGGTGCTG12.5No Hit
CCCACGAGACCCAACACGATATCGTATGCCGTCTTCTGCTTGGAAAAAAA12.5RNA PCR Primer, Index 21 (96% over 26bp)
GCCATGATGATGCCGCCGACCGCCATGATCGGCACGGCCACCATGAAGTC12.5No Hit
GGGCTGATCTCCGCACCGGCGTAGATGGTGCCGCCGTCGAGGCGCACACC12.5No Hit
CTACCGTACAACCCTAACATAACCATTCTTAATTTAACTATTTATATTAT12.5No Hit
CCCCGCCCGGCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCCGTC12.5No Hit
TCGCGATACTCCGTGCCGTGCTCGGCCGTGACGTGCTCCCAGAACGCGGC12.5No Hit
CGCCCAGGTCATGCGCGTCATCACGCGGTCCTGGCGCTGTCTCTTATACA12.5No Hit
CTTCAGGGCCCTGTTCAACTAAGCACTCTACTCTCAGTTTACTGCTAAAT12.5No Hit
ACGCCGGTGCGGGGGGGCCGGTGGAGGTCGCCCGGCACCGGGTCTGTCTC12.5No Hit
ACCAACCACACCTAGCATTCCTGCACATCTGTACCCACGCCTTCTTCAAA12.5No Hit
CTATTTAGGCTTTATGGCCCTGAAGTAGGAACCAGATGTCGGATACGGTT12.5No Hit
CAACCCGTCCTACCTCTCCGAGGGCCTGGCCGTGGTGGACCAGCCGCAGG12.5No Hit
GCCGAGGAGTCCGAGTTCGTGGCATCCGTTCTGGGCCTGTCTCTTATACA12.5No Hit
CCACCCGGCGCCGAGGCAACCGCAGGCAAAGGAGCCAGCACCATGACCGT12.5No Hit
GAACAGCGAGTCGACGAGGCCGTGACGGCGCTCGGTGGTCCAGGCGGGGT12.5No Hit
GGACATGGTTCTTGTTCCCATTGCCCGTGGGGCCACCTGCCTCCCTCACG12.5No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers