FastQCFastQC Report
Wed 25 May 2016
SRR1780558_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780558_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50729
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCAGC150.002213490469.99309525
TGAAGTT200.006916659452.49482339
CTATCGG200.006916659452.49482340
TTCCGTT200.006916659452.49482323
AAGGTCA200.006916659452.49482323
CAACATG200.006916659452.49482333
GTCAGCT200.006916659452.49482326
TTGCGCT200.006916659452.49482353
TCCGTTG200.006916659452.49482324
TTCATAC200.006916659452.4948233
ATCGGGC200.006916659452.49482342
GTAAGGT200.006916659452.49482321
TCCTAAA350.001245055139.99605614
TTAGAAT400.00239892134.9965483
TGCTAAG450.004129482431.3242871
AAAATCG450.00427216131.10804665
GATGCAC802.409619E-630.62197970
CATCTGC604.921041E-429.16379241
GCTAAGA657.858847E-426.9204222
CAGCAGC700.001211148724.99753620
Produced by FastQC (version 0.11.5)