##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780558_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 50729 Sequences flagged as poor quality 0 Sequence length 76 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.596404423505295 34.0 30.0 34.0 12.0 34.0 2 30.97285576297581 34.0 32.0 34.0 23.0 34.0 3 31.09383193045398 34.0 33.0 34.0 23.0 34.0 4 31.58680439196515 34.0 34.0 34.0 23.0 34.0 5 31.56409548778805 34.0 34.0 34.0 23.0 34.0 6 34.09706479528475 38.0 34.0 38.0 23.0 38.0 7 33.583670090086535 37.0 34.0 38.0 21.0 38.0 8 32.70677521733131 37.0 32.0 38.0 11.0 38.0 9 33.26391216069704 37.0 34.0 38.0 21.0 38.0 10-11 33.710520609513296 37.0 34.0 38.0 21.5 38.0 12-13 34.07998383567585 38.0 34.5 38.0 22.5 38.0 14-15 33.6413294170987 37.0 34.0 38.0 21.0 38.0 16-17 33.51796802617832 37.0 34.0 38.0 21.0 38.0 18-19 33.45330087326776 37.0 34.0 38.0 21.0 38.0 20-21 33.5268386918725 37.0 34.0 38.0 21.0 38.0 22-23 33.55324370675551 37.0 34.0 38.0 21.0 38.0 24-25 33.96836129235743 37.5 34.0 38.0 22.0 38.0 26-27 34.0289676516391 38.0 34.0 38.0 22.5 38.0 28-29 34.18011393877269 38.0 34.5 38.0 23.0 38.0 30-31 34.023487551499144 38.0 34.0 38.0 22.5 38.0 32-33 33.76902757791402 37.0 34.0 38.0 22.0 38.0 34-35 33.634883400027604 37.0 34.0 38.0 21.0 38.0 36-37 33.60543278992292 37.0 34.0 38.0 21.0 38.0 38-39 34.144926964852445 38.0 34.5 38.0 23.0 38.0 40-41 33.62882177846991 37.5 34.0 38.0 21.0 38.0 42-43 33.94047783319206 37.0 34.0 38.0 22.5 38.0 44-45 32.96736580654064 37.0 33.5 38.0 16.0 38.0 46-47 33.71129925683534 37.0 34.0 38.0 22.0 38.0 48-49 33.44116777385716 37.0 34.0 38.0 21.0 38.0 50-51 33.40141733525202 37.0 34.0 38.0 21.5 38.0 52-53 33.40710441759151 37.0 34.0 38.0 21.0 38.0 54-55 33.145814031421864 37.0 33.5 38.0 21.0 38.0 56-57 33.2456188767766 37.0 33.5 38.0 21.0 38.0 58-59 33.09235348617162 37.0 33.0 38.0 16.0 38.0 60-61 33.11522009107217 37.0 32.5 38.0 20.5 38.0 62-63 33.23641309704509 37.0 33.0 38.0 20.5 38.0 64-65 33.20293126219717 37.0 33.0 38.0 21.0 38.0 66-67 33.16361450058152 37.0 32.5 38.0 20.5 38.0 68-69 32.76118196692228 37.0 31.5 38.0 15.5 38.0 70-71 32.62352894793905 37.0 31.5 38.0 15.0 38.0 72-73 32.698249521969686 37.0 31.5 38.0 19.0 38.0 74-75 32.313775158193536 37.0 31.0 38.0 15.0 38.0 76 31.53505884208244 36.0 28.0 38.0 11.0 38.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -1.6977642854143475 1101 2 -0.5657838655976377 1101 3 0.1480780145469538 1101 4 -2.205177307538605 1101 5 0.13772965015576233 1101 6 -1.772330308859992 1101 7 -1.3255306516504035 1101 8 -0.7969971606211281 1101 9 -0.32464456533335806 1101 10-11 -0.745299433962721 1101 12-13 -1.290553300149618 1101 14-15 -0.1666391396650866 1101 16-17 -1.9448629408584424 1101 18-19 0.08018073312203455 1101 20-21 -1.026032612047885 1101 22-23 -0.7402944772767341 1101 24-25 0.017474682973301014 1101 26-27 -1.1782041691875378 1101 28-29 -0.3169768745412327 1101 30-31 -1.097984281921157 1101 32-33 0.526282670578702 1101 34-35 -0.5977983153993449 1101 36-37 0.4757034612771065 1101 38-39 -0.6186298024829071 1101 40-41 0.11513062751733827 1101 42-43 -0.7739892870711103 1101 44-45 0.3485068227057937 1101 46-47 0.7232601081584065 1101 48-49 -0.2325613300833922 1101 50-51 -0.19743883124933603 1101 52-53 0.6885808362880681 1101 54-55 -1.1469664466076708 1101 56-57 -0.6639673995995636 1101 58-59 -0.2723505756647171 1101 60-61 0.11144328925077929 1101 62-63 -1.9047038834232914 1101 64-65 0.046725100573716816 1101 66-67 -1.0951134173166324 1101 68-69 0.42474227890284766 1101 70-71 0.8276592425091636 1101 72-73 0.5265259794035302 1101 74-75 -1.0980224029270218 1101 76 -1.8421783044217008 1102 1 -0.7066846579710848 1102 2 -1.160890778886369 1102 3 -0.2708515407462393 1102 4 -0.30526913490315977 1102 5 0.2900974843417785 1102 6 -0.04007266751143845 1102 7 1.2886632352707537 1102 8 0.8875102511590072 1102 9 0.5017361246836956 1102 10-11 -1.0072041958674802 1102 12-13 -5.034354953042175 1102 14-15 -1.1683445050068144 1102 16-17 -3.184761930757432 1102 18-19 -1.248692413932929 1102 20-21 -0.31207485263558254 1102 22-23 -1.3173966680152844 1102 24-25 -0.3733819351560328 1102 26-27 -5.700406714117356 1102 28-29 -0.34150789907224777 1102 30-31 0.1361099067184881 1102 32-33 -7.0814177098489495 1102 34-35 -1.107538575139607 1102 36-37 -0.15619998880815444 1102 38-39 -0.48056080077753904 1102 40-41 -0.3970364982861554 1102 42-43 0.44150986024622085 1102 44-45 -6.158806570971237 1102 46-47 -0.25844000990534255 1102 48-49 -0.2727030065887064 1102 50-51 -6.444913578724087 1102 52-53 -2.117138696704192 1102 54-55 0.1683293686881484 1102 56-57 -1.2500883493568757 1102 58-59 0.7310601550187386 1102 60-61 -0.12245412646481668 1102 62-63 -4.892831917005871 1102 64-65 -1.0471027887087132 1102 66-67 -0.09475266695588402 1102 68-69 -7.634748735133627 1102 70-71 -1.489177960691677 1102 72-73 -0.3684628701307773 1102 74-75 -5.802010334187678 1102 76 -2.2893775698027845 1103 1 -0.1878354516218792 1103 2 -0.4972003026958909 1103 3 0.2757754433807449 1103 4 1.3197308650968402 1103 5 1.3773990716433673 1103 6 -0.9944377468765211 1103 7 1.5882664098739312 1103 8 2.294256282905039 1103 9 1.1307043786519486 1103 10-11 0.8588672327039433 1103 12-13 -1.0794938419310611 1103 14-15 0.24038565372334375 1103 16-17 -0.23337304186854624 1103 18-19 -0.05672812821864426 1103 20-21 -0.17665818596891825 1103 22-23 -0.47215857277718953 1103 24-25 0.7144156838915805 1103 26-27 -0.06895830141894521 1103 28-29 1.3822024183880686 1103 30-31 1.0428559384645268 1103 32-33 -4.475266916198159 1103 34-35 -0.6258917497427774 1103 36-37 0.24756985246168028 1103 38-39 1.0898757071589742 1103 40-41 1.5925468350471803 1103 42-43 1.126033669770031 1103 44-45 -4.146901809066474 1103 46-47 0.14830602184068198 1103 48-49 0.7253128664271671 1103 50-51 0.18703086572035943 1103 52-53 0.049031938216437254 1103 54-55 1.0061269877357617 1103 56-57 1.23453466651614 1103 58-59 1.239988726447308 1103 60-61 1.7540339687732782 1103 62-63 0.9514141147401602 1103 64-65 1.733651179545248 1103 66-67 0.7688386028853884 1103 68-69 -2.9209590525939433 1103 70-71 0.7836394996257852 1103 72-73 1.132529193361286 1103 74-75 -0.6343516040289465 1103 76 0.3564557635305512 1104 1 -1.4578517914911586 1104 2 0.08899250776162404 1104 3 -0.40600560237088956 1104 4 -0.19660900418420368 1104 5 -0.19613034012133923 1104 6 -0.9135553939353471 1104 7 0.87421412229223 1104 8 1.0246484397677875 1104 9 -1.2998511769036085 1104 10-11 -0.793093551609779 1104 12-13 -1.2439137765715884 1104 14-15 -0.9581437580413663 1104 16-17 -0.916788074548279 1104 18-19 -0.16477551383955813 1104 20-21 0.23776174867160904 1104 22-23 -0.7634167427118328 1104 24-25 -0.13505327035679215 1104 26-27 0.2049387574045909 1104 28-29 -1.0248645750759877 1104 30-31 -2.1936636693786156 1104 32-33 -1.9568845632569847 1104 34-35 -0.6028116843833047 1104 36-37 -0.06738112793047435 1104 38-39 -1.1429674300959292 1104 40-41 -0.4150103544953083 1104 42-43 -0.005910774674411812 1104 44-45 -3.7747612861906603 1104 46-47 -0.37293580822467476 1104 48-49 -0.32207275448786277 1104 50-51 0.019138708857617814 1104 52-53 -1.0030513951168913 1104 54-55 -1.678717783506066 1104 56-57 0.2326964312220241 1104 58-59 -0.39154722126510677 1104 60-61 -0.2702715867822789 1104 62-63 -0.6768538591160436 1104 64-65 -1.2361200622848152 1104 66-67 -0.6161695670492549 1104 68-69 -5.281048921874987 1104 70-71 -1.3139912193284644 1104 72-73 0.14560108878612255 1104 74-75 -2.4076359177544404 1104 76 -0.8718939096720639 1105 1 0.09813198132831147 1105 2 0.6444184712504679 1105 3 -0.595153675393199 1105 4 -0.5895603226426225 1105 5 0.9842956233675082 1105 6 0.5357346669165821 1105 7 1.759051850486955 1105 8 0.6594861679625126 1105 9 1.0173902024067374 1105 10-11 1.0478040143131366 1105 12-13 -1.3446997806283818 1105 14-15 -0.36823503593182494 1105 16-17 1.1027859619628728 1105 18-19 0.7408532894058837 1105 20-21 -0.13084879899573565 1105 22-23 0.08914410921514104 1105 24-25 0.48857756128622043 1105 26-27 0.6898779054776014 1105 28-29 -0.7661931754816678 1105 30-31 0.05980996145302697 1105 32-33 0.9915434286294271 1105 34-35 0.8015747253530066 1105 36-37 0.7517365191283574 1105 38-39 0.5848469715267868 1105 40-41 0.10507078140733483 1105 42-43 0.7324512942911028 1105 44-45 1.6986920606653229 1105 46-47 1.0913136846759315 1105 48-49 0.6248339392241036 1105 50-51 0.15240442893874473 1105 52-53 -0.1155513951168885 1105 54-55 -0.8670291425324379 1105 56-57 0.3933708734126924 1105 58-59 -0.0036415417519251037 1105 60-61 0.4034353097694421 1105 62-63 0.725504153054338 1105 64-65 0.1891588423805004 1105 66-67 0.39328783660186417 1105 68-69 1.0772928631148702 1105 70-71 1.543361721848008 1105 72-73 -0.3685723392057625 1105 74-75 -0.8488630982818215 1105 76 -0.6840135851284543 1106 1 -0.7827080497839489 1106 2 -0.22563597521051193 1106 3 -1.708194899142228 1106 4 -1.023731958629142 1106 5 -0.02861596595061755 1106 6 -0.7284603032674966 1106 7 0.8032831183116897 1106 8 0.9142437515767199 1106 9 1.5948856652016516 1106 10-11 0.3298885359620911 1106 12-13 0.17617136274729717 1106 14-15 -0.38555419590071693 1106 16-17 -0.2534753810498316 1106 18-19 -0.23449024183602063 1106 20-21 0.16495104544294747 1106 22-23 -0.10958547336198876 1106 24-25 0.1483390339333539 1106 26-27 -0.2707910081858671 1106 28-29 -2.1539556852042487 1106 30-31 -1.4649761417359812 1106 32-33 0.7009548883131203 1106 34-35 0.168777214334078 1106 36-37 -0.14074468387916284 1106 38-39 -0.12425336552273336 1106 40-41 -0.35463883746744074 1106 42-43 -0.7554408498623815 1106 44-45 0.5657130614431338 1106 46-47 0.13253743370367488 1106 48-49 0.3475977452909902 1106 50-51 0.162438133890781 1106 52-53 -0.7478822221845576 1106 54-55 0.022548249222815286 1106 56-57 0.41616060636576435 1106 58-59 0.2970584841749684 1106 60-61 -0.030140007834909 1106 62-63 0.2057478657844385 1106 64-65 1.286021689152605 1106 66-67 0.9004436571878074 1106 68-69 1.3631627101462414 1106 70-71 0.17644442861492138 1106 72-73 0.5028821591089923 1106 74-75 -2.2535558646805782 1106 76 -2.105584754431021 1107 1 -0.6699260948967307 1107 2 -0.2190570278420907 1107 3 0.826579536947996 1107 4 0.5251402218219887 1107 5 0.3773990716433673 1107 6 0.47266751628137627 1107 7 0.9903131934996594 1107 8 0.8578527741331143 1107 9 -0.7669564400615059 1107 10-11 0.39614793445833385 1107 12-13 0.5347239943262494 1107 14-15 0.04959618003913846 1107 16-17 0.8583667242133259 1107 18-19 1.227445848389543 1107 20-21 0.7091239777737712 1107 22-23 -0.3234012628356737 1107 24-25 0.017230011376959453 1107 26-27 0.2616338038442123 1107 28-29 -0.4641060611441006 1107 30-31 0.10645242969259527 1107 32-33 1.5773646627492113 1107 34-35 1.3633260865145331 1107 36-37 0.8550259928125641 1107 38-39 0.7389985141765081 1107 40-41 0.7078611625325593 1107 42-43 1.0072471200624307 1107 44-45 0.4740777230972668 1107 46-47 1.1574434487412688 1107 48-49 1.3302105272458817 1107 50-51 1.3964606902817636 1107 52-53 1.0199749206725883 1107 54-55 1.1386196777942459 1107 56-57 1.0914425612529826 1107 58-59 1.4037314165057921 1107 60-61 1.2955178869019264 1107 62-63 0.22078545976940234 1107 64-65 0.4993675538142526 1107 66-67 1.3731880180900689 1107 68-69 1.6479371462364654 1107 70-71 0.5255985639532668 1107 72-73 1.1438596027180097 1107 74-75 0.5147712029885909 1107 76 -0.3010734762355334 1108 1 -1.6957120362602076 1108 2 -0.06314922141326562 1108 3 -0.0290810070293972 1108 4 -1.1650752497428307 1108 5 -0.4212586464774368 1108 6 0.3503609108416015 1108 7 0.3118868423869756 1108 8 1.0915157907350164 1108 9 -0.3602538614673634 1108 10-11 0.24798915664130305 1108 12-13 0.06967189259540874 1108 14-15 1.205150754394488 1108 16-17 -0.42991480174923424 1108 18-19 -0.2362816330657722 1108 20-21 0.47241433453817194 1108 22-23 -0.7342763952976981 1108 24-25 -1.0019107516043135 1108 26-27 -1.2625405161840462 1108 28-29 -0.07140030346130288 1108 30-31 0.35353826508644204 1108 32-33 -0.2340756410303726 1108 34-35 -0.27412534407537237 1108 36-37 -0.20531046073742232 1108 38-39 0.48822581901578843 1108 40-41 0.17697543310832486 1108 42-43 0.5565892253255882 1108 44-45 0.07159185238020882 1108 46-47 0.22669901214642607 1108 48-49 0.2062039924525223 1108 50-51 -0.20628568908949063 1108 52-53 0.040153302869683216 1108 54-55 0.20385722636365955 1108 56-57 0.936925606113455 1108 58-59 -0.6770041892872172 1108 60-61 0.8400472051640335 1108 62-63 -0.45989274581166484 1108 64-65 0.15474551213287668 1108 66-67 -0.7061567364136394 1108 68-69 1.1510720808885324 1108 70-71 1.7353080305728383 1108 72-73 0.48958549462899725 1108 74-75 0.9292669642037126 1108 76 0.0790042348206299 1109 1 -0.3115987697345126 1109 2 -0.620012327049011 1109 3 -0.5363440391154484 1109 4 -0.31516259000209246 1109 5 0.1239744141091208 1109 6 0.7846718421645775 1109 7 -0.14099538616869012 1109 8 0.6498498902109802 1109 9 1.10425841213749 1109 10-11 0.7923717989139973 1109 12-13 0.12317929353316259 1109 14-15 0.0451801742712874 1109 16-17 -0.5451120068609328 1109 18-19 0.2912075643232086 1109 20-21 0.8424152539093228 1109 22-23 0.3893330558377244 1109 24-25 0.47816187871654137 1109 26-27 0.17462046570433643 1109 28-29 -0.10776143244907388 1109 30-31 -0.1494956597089896 1109 32-33 0.5512741796922498 1109 34-35 0.014281385721446327 1109 36-37 -0.20648265895384554 1109 38-39 0.32303780761559153 1109 40-41 0.059203988776246774 1109 42-43 0.3819316910790107 1109 44-45 -0.5905852185033069 1109 46-47 -0.4511993054043728 1109 48-49 -0.7585151396611067 1109 50-51 0.5703071214281223 1109 52-53 0.13307874186940438 1109 54-55 -0.30014203813941975 1109 56-57 -0.08353040197700778 1109 58-59 -0.34324947751007073 1109 60-61 0.35900923513552385 1109 62-63 -0.5589549872385291 1109 64-65 -0.6252719559189828 1109 66-67 0.22151173834961924 1109 68-69 0.9522810539509621 1109 70-71 0.15911514650554182 1109 72-73 -0.266919056258196 1109 74-75 0.5697009073865758 1109 76 -1.6828326687373334 1110 1 0.4382814869950593 1110 2 0.7438156258200372 1110 3 0.38266161276691335 1110 4 0.5843967547627322 1110 5 0.40537109961539386 1110 6 0.8875552601164856 1110 7 1.5829245295320504 1110 8 2.6162259798747414 1110 9 -0.7124385894910219 1110 10-11 -0.08795677662006085 1110 12-13 0.25585483716761104 1110 14-15 0.05681922015691043 1110 16-17 0.5516405556450508 1110 18-19 1.0785914133508996 1110 20-21 -0.39793343849416374 1110 22-23 0.8747722741536563 1110 24-25 0.6007550389809353 1110 26-27 -0.04120480491545209 1110 28-29 1.431274873710521 1110 30-31 1.312378082986669 1110 32-33 1.6143309858997483 1110 34-35 0.7588450412440153 1110 36-37 0.47061763800947176 1110 38-39 0.6229468027300626 1110 40-41 1.3071204558708018 1110 42-43 1.1300157987521615 1110 44-45 1.6425309803663168 1110 46-47 0.7165849651196226 1110 48-49 1.3656586317729378 1110 50-51 -0.2009353347458429 1110 52-53 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1.2767017553160542 2116 50-51 0.9245308657203566 2116 52-53 1.9469486048831115 2116 54-55 1.3651547655135445 2116 56-57 0.6032846665161458 2116 58-59 0.19276650422509078 2116 60-61 -1.4327715867822803 2116 62-63 1.1903030036290545 2116 64-65 1.7197622906563623 2116 66-67 0.6806441584409413 2116 68-69 0.6589020585171639 2116 70-71 -2.3524716114853277 2116 72-73 0.9103069711390646 2116 74-75 -1.080184937362283 2116 76 -1.0699331253583395 2117 1 0.26962982615589937 2117 2 0.6078344195263305 2117 3 0.5613657211585235 2117 4 -0.3165538571253812 2117 5 -1.5522884283566327 2117 6 -1.705375246876521 2117 7 -2.327532201237183 2117 8 -2.4270978837616255 2117 9 -0.2998511769036085 2117 10-11 -0.643736933962721 2117 12-13 0.7742387969578246 2117 14-15 -0.26352059627665625 2117 16-17 -0.2950049863129891 2117 18-19 -0.21601632266308712 2117 20-21 0.14539042514219602 2117 22-23 0.4401678161116962 2117 24-25 0.7413254061138019 2117 26-27 0.6966666985810548 2117 28-29 0.4820288072769543 2117 30-31 -0.37771697820214456 2117 32-33 0.40363933380184136 2117 34-35 0.09502838914610834 2117 36-37 -2.0623259808716483 2117 38-39 -0.4596034595076972 2117 40-41 -1.0094496927305983 2117 42-43 -1.5410670246744118 2117 44-45 0.8821780520446367 2117 46-47 0.22426088295179625 2117 48-49 -0.3949128280172758 2117 50-51 0.2521350323870237 2117 52-53 0.5555423548831087 2117 54-55 -1.546824401153124 2117 56-57 -0.7342153334838599 2117 58-59 -0.7035876624415778 2117 60-61 0.5539471632177211 2117 62-63 -1.5047490797042826 2117 64-65 -0.7255502093436377 2117 66-67 -1.2524287582257259 2117 68-69 0.14197497518383528 2117 70-71 0.9589867218480066 2117 72-73 0.10561947113906456 2117 74-75 -1.2619557706956144 2117 76 -1.472537292025006 2118 1 -0.012634009721963935 2118 2 -0.04644754612252555 2118 3 0.013953984517300455 2118 4 0.22823383371431305 2118 5 0.03388762126168743 2118 6 -0.3713461438230894 2118 7 1.5887965201368601 2118 8 -4.831379849410482 2118 9 -1.5670267494226913 2118 10-11 -1.1956811133520375 2118 12-13 -0.030538159149045896 2118 14-15 -2.2309883920781814 2118 16-17 0.3417435823892987 2118 18-19 -1.300552343655454 2118 20-21 -3.992312342033376 2118 22-23 -0.1825063251096779 2118 24-25 -0.32982201755032037 2118 26-27 0.01954360697800439 2118 28-29 -1.0206548759291536 2118 30-31 -0.18965042285863376 2118 32-33 -0.09692722154166944 2118 34-35 0.29186164296289974 2118 36-37 -1.304752030489972 2118 38-39 -0.6087566083626612 2118 40-41 -2.0914809427305983 2118 42-43 -0.733487110552268 2118 44-45 -0.2838823010088021 2118 46-47 -1.1308500426207218 2118 48-49 -1.4586353948111679 2118 50-51 0.07459447895190863 2118 52-53 -1.6705132271779597 2118 54-55 0.6389461141140558 2118 56-57 0.3115861932336941 2118 58-59 0.4021494558790266 2118 60-61 -1.4644509760952573 2118 62-63 0.6219505863262498 2118 64-65 -0.39712702232073127 2118 66-67 -3.1020530425768698 2118 68-69 0.5469427709853605 2118 70-71 -1.144424537693979 2118 72-73 -2.3707044792426153 2118 74-75 -0.4560755226040101 2118 76 -1.1146532271395095 2119 1 1.3614759396012772 2119 2 1.5333202388540634 2119 3 1.8229859942677678 2119 4 1.1699876344712585 2119 5 1.1505083153408435 2119 6 2.4015706564848216 2119 7 -1.530197642413654 2119 8 1.8072114649778683 2119 9 0.2715773945249609 2119 10-11 1.258902246709546 2119 12-13 1.3648047108233712 2119 14-15 -0.03482443031027316 2119 16-17 0.08951444646011453 2119 18-19 0.2648463348999357 2119 20-21 -0.8914399320006581 2119 22-23 1.0974025850192533 2119 24-25 1.2830139565339636 2119 26-27 1.2984261523625662 2119 28-29 1.7261858450920826 2119 30-31 0.8205170028902913 2119 32-33 1.3256784619531032 2119 34-35 1.4449824332637604 2119 36-37 0.683669292237596 2119 38-39 0.8817174438536455 2119 40-41 0.5261661160929307 2119 42-43 0.47675729255248456 2119 44-45 -0.6996562441738519 2119 46-47 -0.39709547209022134 2119 48-49 -0.07942569566433377 2119 50-51 1.7418277844878602 2119 52-53 -0.5172320673857982 2119 54-55 0.045546922376281884 2119 56-57 1.3666250026505935 2119 58-59 1.9766250476424574 2119 60-61 1.299581354394192 2119 62-63 0.5348058047494959 2119 64-65 1.244447164605944 2119 66-67 0.5408682480767979 2119 68-69 -0.476952283219525 2119 70-71 -0.3465542445385452 2119 72-73 -1.2078652977684996 2119 74-75 1.0261876116573276 2119 76 2.160669115538017 >>END_MODULE >>Per sequence quality scores pass #Quality Count 12 13.0 13 56.0 14 188.0 15 359.0 16 445.0 17 535.0 18 567.0 19 577.0 20 501.0 21 460.0 22 466.0 23 456.0 24 481.0 25 650.0 26 685.0 27 810.0 28 989.0 29 1174.0 30 1501.0 31 1944.0 32 2365.0 33 3136.0 34 4235.0 35 6203.0 36 10264.0 37 11669.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 33.92609058071687 22.702734886674868 11.7096018735363 31.66157265907196 2 16.85820733702616 20.455755090776478 38.75101027025962 23.935027301937748 3 16.28062843738296 25.027104811843326 31.5026907685939 27.18957598217982 4 12.286857615959313 20.148238680044944 39.048670385775395 28.516233318220348 5 12.67125312937373 37.39872656665813 35.49251907193124 14.437501232036901 6 25.68156281416941 37.974334207258174 18.14346823316052 18.200634745411897 7 24.83392142561454 31.490863214334997 23.424471209761673 20.250744150288792 8 21.98939462634785 37.416467898046484 19.373533876086658 21.220603599519013 9 22.878432454808888 16.588144848114492 22.659622701019142 37.873799996057485 10-11 24.377574957125116 26.41289991917838 27.16099272605413 22.048532397642372 12-13 23.01149244022157 23.95178300380453 26.63860907961915 26.39811547635475 14-15 21.412801356226222 25.264641526543002 24.00993514557748 29.312621971653297 16-17 21.816909460072146 27.910071162451455 25.865875534704013 24.407143842772378 18-19 21.38520372962211 27.434997733052104 26.202960831082812 24.97683770624298 20-21 20.89140333931282 27.682390742967534 26.750970845078754 24.6752350726409 22-23 22.743401210353053 26.912614086617122 26.450353840998247 23.89363086203158 24-25 22.070216247117035 28.203788759880936 26.060044550454375 23.665950442547658 26-27 20.771156537680614 28.128880916241204 27.047645331072957 24.05231721500522 28-29 21.85337775237044 27.066372291982887 28.016519150781605 23.063730804865067 30-31 21.719332137436183 27.17577716887776 27.642965562104514 23.46192513158154 32-33 20.61247018470697 28.41668473654123 27.221116126870232 23.749728951881565 34-35 21.923357448402296 27.56312957085691 27.136351988014745 23.377160992726054 36-37 22.291982889471505 27.266455084862702 26.875160164797258 23.566401860868535 38-39 21.16048019870291 27.656764375406574 28.020461668867906 23.16229375702261 40-41 21.677935697530014 26.433598139131465 27.87360287015317 24.014863293185357 42-43 21.12006938831832 26.1152398036626 28.35459007668198 24.410100731337106 44-45 20.514892862071004 26.424727473437283 28.13380906384908 24.92657060064263 46-47 21.10725620453784 26.24238601194583 28.50144887539672 24.148908908119616 48-49 20.096985944923023 26.38037414496639 29.0396025941769 24.483037315933686 50-51 19.80819649510142 26.700703739478403 28.432454808886437 25.058644956533737 52-53 19.414930315992823 26.555816199806813 28.91442764493682 25.11482583926354 54-55 19.035462950186282 26.908671568530824 28.649293303633026 25.406572177649867 56-57 18.877762226734216 26.732243884168817 28.743913737704275 25.6460801513927 58-59 19.51940704527982 26.79926669163595 28.194918094186754 25.486408168897473 60-61 19.62782629265312 26.543003016026333 27.805594433164458 26.023576258156083 62-63 19.823966567446625 25.94472589643005 28.0007490784364 26.230558457686925 64-65 20.40844487374086 26.415856807743104 27.26054130773325 25.915157010782785 66-67 20.40351672613298 26.76082714029451 26.62776715488182 26.20788897869069 68-69 19.520392674801396 26.775611583118135 26.66423544718011 27.03976029490035 70-71 21.231445524256344 26.18226261112973 25.91220012221806 26.674091742395866 72-73 21.075716059847423 26.249285418596855 25.72690177216188 26.94809674939384 74-75 20.732716986339174 26.43655502769619 25.895444420351275 26.935283565613354 76 21.50840741981904 25.717045476946126 26.036389441936564 26.738157661298274 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.5 8 1.0 9 1.0 10 1.5 11 1.0 12 0.0 13 0.5 14 1.5 15 3.0 16 4.0 17 4.0 18 3.5 19 6.5 20 12.5 21 15.0 22 24.5 23 34.5 24 40.0 25 45.0 26 97.5 27 168.5 28 232.0 29 277.0 30 311.0 31 423.5 32 612.5 33 723.0 34 861.5 35 1159.0 36 1376.5 37 1435.0 38 1671.0 39 2159.5 40 2649.0 41 3049.0 42 3212.0 43 3332.5 44 3566.0 45 3729.5 46 3780.0 47 3869.5 48 3752.0 49 3399.5 50 3254.0 51 3012.0 52 2491.5 53 2024.0 54 1835.0 55 1714.0 56 1386.5 57 1032.5 58 885.0 59 725.5 60 490.0 61 348.5 62 283.0 63 254.0 64 202.0 65 154.5 66 102.5 67 75.0 68 63.5 69 51.5 70 40.0 71 29.0 72 22.5 73 12.0 74 7.5 75 7.0 76 5.5 77 4.0 78 5.5 79 7.0 80 4.5 81 3.0 82 2.5 83 1.0 84 2.5 85 2.0 86 0.5 87 1.0 88 1.0 89 1.5 90 2.5 91 1.5 92 0.0 93 0.0 94 1.0 95 1.0 96 0.0 97 0.0 98 0.0 99 1.0 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.6761418518007452 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 50729.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.78631551972244 #Duplication Level Percentage of deduplicated Percentage of total 1 75.14589996373108 44.92696485245126 2 10.676250453361469 12.765873563444972 3 5.667842658841373 10.165782885528987 4 2.8751360084407662 6.875751542510201 5 1.8002571795970854 5.381537187801849 6 1.2826008111048832 4.600918606714108 7 0.6891094332157341 2.8839519801297087 8 0.48138745095453195 2.302430562400205 9 0.32642025783903195 1.7563918074474167 >10 1.0550957829140426 8.34039701157129 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0019712590431508604 0.0 2 0.0 0.0 0.0 0.0019712590431508604 0.0 3 0.0 0.0 0.0 0.0019712590431508604 0.0 4 0.0 0.0 0.0 0.0019712590431508604 0.0 5 0.0 0.0 0.0 0.003942518086301721 0.0 6 0.0 0.0 0.0 0.003942518086301721 0.0 7 0.0 0.0 0.0 0.003942518086301721 0.0 8 0.0 0.0 0.0 0.003942518086301721 0.0 9 0.0 0.0 0.0 0.015770072345206883 0.0 10 0.0 0.0 0.0 0.017741331388357744 0.0 11 0.0 0.0 0.0 0.019712590431508605 0.0 12 0.0 0.0 0.0 0.019712590431508605 0.0 13 0.0 0.0 0.0 0.019712590431508605 0.0 14 0.0 0.0 0.0 0.021683849474659466 0.0 15 0.0 0.0 0.0 0.03154014469041377 0.0 16 0.0 0.0 0.0 0.03154014469041377 0.0 17 0.0 0.0 0.0 0.03745392181986635 0.0 18 0.0 0.0 0.0 0.04336769894931893 0.0 19 0.0 0.0 0.0 0.04533895799246979 0.0 20 0.0 0.0 0.0 0.05322399416507323 0.0 21 0.0 0.0 0.0 0.05519525320822409 0.0 22 0.0 0.0 0.0 0.0650515484239784 0.0 23 0.0 0.0 0.0 0.06702280746712926 0.0 24 0.0 0.0 0.0 0.06899406651028012 0.0 25 0.0 0.0 0.0 0.07687910268288356 0.0 26 0.0 0.0 0.0 0.07885036172603442 0.0 27 0.0 0.0 0.0 0.084764138855487 0.0 28 0.0 0.0 0.0 0.09659169311439217 0.0 29 0.0 0.0 0.0 0.12616057876165507 0.0 30 0.0 0.0 0.0 0.15375820536576712 0.0 31 0.0 0.0 0.0 0.17544205484042658 0.0 32 0.0 0.0 0.0 0.2286660490054998 0.0 33 0.0 0.0 0.0 0.27794752508427134 0.0 34 0.0 0.0 0.0 0.31145892881783593 0.0 35 0.0 0.0 0.0 0.4139643990616807 0.0 36 0.0 0.0 0.0 0.5775788996432021 0.0 37 0.0 0.0 0.0 0.8712964970726803 0.0 38 0.0 0.0 0.0 1.2616057876165507 0.0 39 0.0 0.0 0.0 1.7189378856275503 0.0 40 0.0 0.0 0.0 2.272861676752942 0.0 41 0.0 0.0 0.0 2.994342486546157 0.0 42 0.0 0.0 0.0 3.755248477202389 0.0 43 0.0 0.0 0.0 4.478700546038755 0.0 44 0.0 0.0 0.0 5.192296319659366 0.0 45 0.0 0.0 0.0 6.039937708214237 0.0 46 0.0 0.0 0.0 6.822527548345128 0.0 47 0.0 0.0 0.0 7.646513828382187 0.0 48 0.0 0.0 0.0 8.40544855999527 0.0 49 0.0 0.0 0.0 9.083561670839165 0.0 50 0.0 0.0 0.0 9.816870034891284 0.0 51 0.0 0.0 0.0 10.599459875022177 0.0 52 0.0 0.0 0.0 11.301228094383884 0.0 53 0.0 0.0 0.0 11.993140018529836 0.0 54 0.0 0.0 0.0 12.588460249561395 0.0 55 0.0 0.0 0.0 13.191665516765559 0.0 56 0.0 0.0 0.0 13.684480277553273 0.0 57 0.0 0.0 0.0 14.25023162293757 0.0 58 0.0 0.0 0.0 14.886948293875298 0.0 59 0.0 0.0 0.0 15.468469711604802 0.0 60 0.0 0.0 0.0 15.998738394212383 0.0 61 0.0 0.0 0.0 16.434386642748724 0.0 62 0.0 0.0 0.0 17.008023024305626 0.0 63 0.0 0.0 0.0 17.634883400027597 0.0 64 0.0 0.0 0.0 18.25385873957697 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTCAGC 15 0.0022134904 69.993095 25 TGAAGTT 20 0.0069166594 52.494823 39 CTATCGG 20 0.0069166594 52.494823 40 TTCCGTT 20 0.0069166594 52.494823 23 AAGGTCA 20 0.0069166594 52.494823 23 CAACATG 20 0.0069166594 52.494823 33 GTCAGCT 20 0.0069166594 52.494823 26 TTGCGCT 20 0.0069166594 52.494823 53 TCCGTTG 20 0.0069166594 52.494823 24 TTCATAC 20 0.0069166594 52.494823 3 ATCGGGC 20 0.0069166594 52.494823 42 GTAAGGT 20 0.0069166594 52.494823 21 TCCTAAA 35 0.0012450551 39.996056 14 TTAGAAT 40 0.002398921 34.996548 3 TGCTAAG 45 0.0041294824 31.324287 1 AAAATCG 45 0.004272161 31.108046 65 GATGCAC 80 2.409619E-6 30.621979 70 CATCTGC 60 4.921041E-4 29.163792 41 GCTAAGA 65 7.858847E-4 26.920422 2 CAGCAGC 70 0.0012111487 24.997536 20 >>END_MODULE