FastQCFastQC Report
Wed 25 May 2016
SRR1780558_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780558_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50729
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGT7201.4193065110686196No Hit
ATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGTCTT4220.8318713162096631Illumina PCR Primer Index 9 (95% over 21bp)
ATAAAGAATATTGAGGCGCCATTGGCGTGAAGGTAGCGGATGATTCAGCC920.18135583196987914No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCC740.14587316919316368No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCAATA150.002212624870.053
GTACATG207.855863E-570.01
TTCACGC150.002212624870.037
CACGCCA150.002212624870.039
GCGCCTC150.002212624870.049
AATGGCG150.002212624870.045
TACATGC207.855863E-570.02
CTTTATC150.002212624870.062
GCCAATG150.002212624870.042
CGCCAAT150.002212624870.041
CTTGTAC252.3692285E-455.9999963
ATCCTAC252.3692285E-455.99999655
AACAAGT252.3692285E-455.99999670
CGGAACA252.3692285E-455.99999667
GCGAACT252.3692285E-455.99999628
ACGGAAC252.3692285E-455.99999666
TACCTTC252.3692285E-455.99999633
AGAATAT252.3692285E-455.9999965
AAAGCGA252.3692285E-455.99999625
CAAAGCG252.3692285E-455.99999624
Produced by FastQC (version 0.11.5)