##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780558_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 50729 Sequences flagged as poor quality 0 Sequence length 76 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.89601608547379 34.0 34.0 34.0 31.0 34.0 2 33.33700644601707 34.0 34.0 34.0 33.0 34.0 3 33.47828658163969 34.0 34.0 34.0 34.0 34.0 4 33.51501113761359 34.0 34.0 34.0 34.0 34.0 5 33.51615446785862 34.0 34.0 34.0 34.0 34.0 6 37.25147351613476 38.0 37.0 38.0 36.0 38.0 7 36.84823276626781 38.0 37.0 38.0 35.0 38.0 8 36.86508703108675 38.0 37.0 38.0 35.0 38.0 9 36.65016065761202 38.0 37.0 38.0 34.0 38.0 10-11 36.334887342545684 38.0 37.0 38.0 33.0 38.0 12-13 36.23669892960634 38.0 37.0 38.0 34.0 38.0 14-15 36.480464822882375 38.0 37.0 38.0 34.0 38.0 16-17 36.62316426501607 38.0 37.0 38.0 34.0 38.0 18-19 36.60734491119478 38.0 37.0 38.0 34.0 38.0 20-21 36.340032328648306 38.0 37.0 38.0 34.0 38.0 22-23 36.38864357665241 38.0 37.0 38.0 34.0 38.0 24-25 35.7261034122494 38.0 36.5 38.0 31.5 38.0 26-27 35.963679552129946 38.0 37.0 38.0 33.0 38.0 28-29 35.81137022216089 38.0 36.5 38.0 31.5 38.0 30-31 35.52174298724596 38.0 36.0 38.0 30.5 38.0 32-33 36.03343255337184 38.0 37.0 38.0 33.0 38.0 34-35 36.063316840466 38.0 37.0 38.0 33.0 38.0 36-37 36.17246545368527 38.0 37.0 38.0 34.0 38.0 38-39 35.795205898007055 38.0 36.5 38.0 32.5 38.0 40-41 35.31045358670583 38.0 36.0 38.0 29.0 38.0 42-43 35.80628437382956 38.0 36.5 38.0 32.5 38.0 44-45 35.97734037729898 38.0 37.0 38.0 33.0 38.0 46-47 36.284994776163536 38.0 37.0 38.0 34.0 38.0 48-49 36.100199097163355 38.0 37.0 38.0 33.5 38.0 50-51 36.2328549744722 38.0 37.0 38.0 34.0 38.0 52-53 36.11015395533127 38.0 37.0 38.0 34.0 38.0 54-55 36.33324134124465 38.0 37.0 38.0 34.0 38.0 56-57 36.412771787340574 38.0 37.0 38.0 34.0 38.0 58-59 36.46355142029214 38.0 37.0 38.0 34.0 38.0 60-61 36.12474127225059 38.0 37.0 38.0 33.0 38.0 62-63 36.393502730193774 38.0 37.0 38.0 34.0 38.0 64-65 36.39267480139565 38.0 37.0 38.0 34.0 38.0 66-67 35.73241144118748 38.0 36.5 38.0 31.5 38.0 68-69 35.134035758639044 38.0 36.0 38.0 29.0 38.0 70-71 35.08693252380296 38.0 36.0 38.0 27.5 38.0 72-73 34.80350489857872 38.0 35.5 38.0 26.0 38.0 74-75 35.44383882986063 38.0 36.5 38.0 31.0 38.0 76 34.89562183366516 37.0 36.0 38.0 27.0 38.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.3489253311035512 1101 2 0.5396611010919017 1101 3 0.15476389469227314 1101 4 0.41666426601921813 1101 5 0.36968459462670467 1101 6 -1.186052781367465 1101 7 0.2430765501328338 1101 8 0.6669852248953632 1101 9 -0.13550658261227255 1101 10-11 -0.3397264510722522 1101 12-13 0.7921938797667138 1101 14-15 0.7068211086609963 1101 16-17 -0.2983344146444651 1101 18-19 -0.8626404330581963 1101 20-21 -0.4616127051239758 1101 22-23 0.3695410787931408 1101 24-25 -2.8515981877655605 1101 26-27 -1.1594726381678697 1101 28-29 0.789450289176564 1101 30-31 1.170404930521265 1101 32-33 0.716990204127498 1101 34-35 0.5349766733727463 1101 36-37 0.5493579007903477 1101 38-39 0.3771753705085885 1101 40-41 1.1717657169890856 1101 42-43 0.7982726257796671 1101 44-45 0.5542376847162913 1101 46-47 0.44530556095038776 1101 48-49 -1.5243993343617035 1101 50-51 -1.3724261125965 1101 52-53 -1.7839875687347089 1101 54-55 -0.5846491945387626 1101 56-57 -1.2116863501322115 1101 58-59 -0.5518774603839063 1101 60-61 -1.8357397008839982 1101 62-63 -0.7498781680554316 1101 64-65 -1.2370154717923398 1101 66-67 -0.9256148854060768 1101 68-69 -0.8206998152465488 1101 70-71 -1.0487688212242716 1101 72-73 -1.2600770621516233 1101 74-75 0.47416518139244346 1101 76 -0.1117914452711588 1102 1 -0.7591030041975131 1102 2 0.07632645593907483 1102 3 0.15463271274290946 1102 4 0.19640977303745188 1102 5 -0.15182924506896 1102 6 0.22664563133094617 1102 7 -0.12495440880721276 1102 8 0.3120068699170062 1102 9 0.4883291775871186 1102 10-11 0.41870198917437307 1102 12-13 0.5043478873752605 1102 14-15 -0.3111508184018419 1102 16-17 0.14984871535684618 1102 18-19 -0.2803893508071127 1102 20-21 0.6348716186330776 1102 22-23 0.6244276064069396 1102 24-25 -4.360420173860277 1102 26-27 -1.0634802467209283 1102 28-29 -0.27981378008750823 1102 30-31 1.2347824721715384 1102 32-33 0.2975687165241965 1102 34-35 0.5955171430041233 1102 36-37 -2.028793745543112 1102 38-39 -0.4496644563312415 1102 40-41 0.3887078657494101 1102 42-43 0.015050797103292268 1102 44-45 0.8392632651964149 1102 46-47 0.9529797049881594 1102 48-49 -3.08159466428431 1102 50-51 -0.606126755388047 1102 52-53 -0.2662124145959197 1102 54-55 0.8153901600460429 1102 56-57 0.8382939725753857 1102 58-59 0.0778614875368504 1102 60-61 -5.214953921007314 1102 62-63 -2.4478973206200365 1102 64-65 0.04263164876387293 1102 66-67 0.5936688611503911 1102 68-69 -0.6922726868194147 1102 70-71 -1.6180000950010012 1102 72-73 -6.935467328450983 1102 74-75 -2.7905599924236455 1102 76 -0.920724936022836 1103 1 0.6307779481834359 1103 2 0.38585026546288503 1103 3 0.030624776234972728 1103 4 0.42458437621206 1103 5 0.4136469454072369 1103 6 0.2792249964103135 1103 7 0.8700852737324638 1103 8 0.7187529016630378 1103 9 0.14904346330140328 1103 10-11 0.27683690980929754 1103 12-13 -0.4490251284977518 1103 14-15 0.09906743556641118 1103 16-17 0.23963046138859312 1103 18-19 0.6600868396690771 1103 20-21 0.21423669799815315 1103 22-23 0.5857371302164651 1103 24-25 -2.58016223735234 1103 26-27 -0.3189365959272763 1103 28-29 0.07732907705534586 1103 30-31 1.003135646774716 1103 32-33 1.1586798276353107 1103 34-35 0.4467076191946049 1103 36-37 -1.0402024757018395 1103 38-39 -0.3807160436328232 1103 40-41 -0.15295880091726133 1103 42-43 0.42427698757948207 1103 44-45 1.567437868371023 1103 46-47 1.1345273240357798 1103 48-49 -1.676832759522405 1103 50-51 -0.36654342205471835 1103 52-53 1.0021407600072578 1103 54-55 0.24842587433175112 1103 56-57 0.3943455598769745 1103 58-59 -0.03176152833616186 1103 60-61 -4.397493603546998 1103 62-63 -1.1205163682390875 1103 64-65 0.5753697440019678 1103 66-67 -0.03133113884960892 1103 68-69 -0.10100284554957994 1103 70-71 0.4559086351577335 1103 72-73 -4.938939550673204 1103 74-75 -2.3614925321061833 1103 76 -3.3413598566577534 1104 1 0.1442583403403006 1104 2 0.3989221608877216 1104 3 0.33086987427419245 1104 4 0.26772163111402136 1104 5 -0.00628769511564542 1104 6 0.019829571573708904 1104 7 0.27899050249063606 1104 8 -0.010822261735654592 1104 9 0.6939781038242856 1104 10-11 -0.7133591686220768 1104 12-13 -0.0335839520271648 1104 14-15 0.5465756055010544 1104 16-17 0.09808634374153513 1104 18-19 0.4027338984926061 1104 20-21 -0.4875606876227607 1104 22-23 0.13679922171973402 1104 24-25 0.40329364500060194 1104 26-27 0.7213003321773002 1104 28-29 0.6667898613690753 1104 30-31 0.6177189801080445 1104 32-33 0.051245187112428425 1104 34-35 -0.32984466838709636 1104 36-37 1.0784658249517491 1104 38-39 0.9209587929684773 1104 40-41 1.2600313951611781 1104 42-43 -0.036711574512018785 1104 44-45 0.5633529010507559 1104 46-47 0.23869399070245123 1104 48-49 -0.7752804719407038 1104 50-51 0.36772945376227995 1104 52-53 0.37571102144517 1104 54-55 -0.4213045178251136 1104 56-57 -0.07277045319492004 1104 58-59 -1.0552500904276627 1104 60-61 0.05879724612621118 1104 62-63 0.20709801084588264 1104 64-65 0.0990625544594792 1104 66-67 -0.3021644721829375 1104 68-69 1.3891932328817944 1104 70-71 1.1632207266610024 1104 72-73 0.6200882271045742 1104 74-75 -0.18440919877284756 1104 76 -0.8233860004486075 1105 1 0.612818178068494 1105 2 0.3325935604820458 1105 3 0.2962856957752038 1105 4 0.1765958704649293 1105 5 -0.5586710622555984 1105 6 0.3380851496670161 1105 7 0.7549032813953005 1105 8 0.259461713923578 1105 9 0.9644649192401005 1105 10-11 0.45542886383228165 1105 12-13 -0.4235940940149945 1105 14-15 0.09530785702234823 1105 16-17 0.21657011656100877 1105 18-19 0.4238799431173561 1105 20-21 0.6766888052778484 1105 22-23 0.38578502293676564 1105 24-25 0.8847084523028315 1105 26-27 0.6216045918121864 1105 28-29 0.957788847170292 1105 30-31 0.972029324935626 1105 32-33 0.9268790613517837 1105 34-35 -0.6531965953647827 1105 36-37 0.4730398614629081 1105 38-39 0.6304669065587447 1105 40-41 0.8111216588528549 1105 42-43 0.4024618534798705 1105 44-45 0.5817098990223641 1105 46-47 0.9490388182886562 1105 48-49 0.2860024895197455 1105 50-51 0.9865935511253596 1105 52-53 0.8122221776317744 1105 54-55 0.8239287478949748 1105 56-57 0.5926453683060942 1105 58-59 0.3934060961849113 1105 60-61 0.5046136761464908 1105 62-63 -0.7985815023386991 1105 64-65 0.3997471386379772 1105 66-67 0.32930104505844326 1105 68-69 0.8156638211170915 1105 70-71 1.3859134244297664 1105 72-73 1.2718123650356148 1105 74-75 0.21688390467542717 1105 76 0.3444638981315151 1106 1 -0.7553436057012703 1106 2 -0.5694546635262512 1106 3 0.2431874328765815 1106 4 0.03767961430729372 1106 5 -0.5123137897640291 1106 6 -0.35898511219453155 1106 7 -1.6734088783143193 1106 8 -0.25440917018324427 1106 9 -1.1035672300987613 1106 10-11 -0.8198109598648884 1106 12-13 0.7497530669909693 1106 14-15 -1.043815814224729 1106 16-17 -0.27329247094223064 1106 18-19 0.29354548628562327 1106 20-21 -0.3730648893034285 1106 22-23 0.10020037165339346 1106 24-25 -0.35436336517189204 1106 26-27 0.6911145736633628 1106 28-29 0.4996348414663814 1106 30-31 0.45888439364188116 1106 32-33 -0.042656847385572405 1106 34-35 0.5245062825195816 1106 36-37 0.676928894390052 1106 38-39 -0.35317322826106334 1106 40-41 0.6161514747719679 1106 42-43 0.01672164087940331 1106 44-45 0.5804913353718533 1106 46-47 0.471776697469366 1106 48-49 0.6072106824157757 1106 50-51 -0.8722086267330766 1106 52-53 -0.4495875190236447 1106 54-55 -0.7021536996030804 1106 56-57 -0.13789650361508876 1106 58-59 -0.02907251413398626 1106 60-61 0.35167097456495355 1106 62-63 -0.007629985615857038 1106 64-65 -1.4613366052043801 1106 66-67 0.027252821050140597 1106 68-69 0.6389720917937822 1106 70-71 -0.47159240911962996 1106 72-73 -0.3122425999630991 1106 74-75 -0.5269843867427753 1106 76 -0.543531953566692 1107 1 0.842400755200984 1107 2 -0.2047930093909187 1107 3 0.2554054779893633 1107 4 -0.014012115016015514 1107 5 -0.34877995517756233 1107 6 0.37973669231674734 1107 7 0.8265911216856807 1107 8 -0.14820616266444375 1107 9 0.3456808902019901 1107 10-11 0.5838544536689412 1107 12-13 0.370053818870673 1107 14-15 0.7414285466775254 1107 16-17 0.7675909877043807 1107 18-19 0.9331131554585497 1107 20-21 1.016502779869505 1107 22-23 0.3953131536082779 1107 24-25 0.6813509205423998 1107 26-27 0.5891408894528354 1107 28-29 0.9545220595114898 1107 30-31 0.7953505590554144 1107 32-33 0.7646739796820867 1107 34-35 0.2204649291361207 1107 36-37 1.0345416763449435 1107 38-39 0.6947591025660032 1107 40-41 1.3360762868020402 1107 42-43 1.3170035957666215 1107 44-45 1.0447770496575757 1107 46-47 0.9163255696498211 1107 48-49 1.1415151936939694 1107 50-51 1.1376597943195534 1107 52-53 0.6711831576680751 1107 54-55 0.8114741199457924 1107 56-57 0.8024230452570862 1107 58-59 0.5846492903772855 1107 60-61 1.1251672151664494 1107 62-63 0.8659602399480448 1107 64-65 0.7350919662241893 1107 66-67 0.2504671067644324 1107 68-69 0.6643480316434065 1107 70-71 0.04673465854954628 1107 72-73 0.9687724376308893 1107 74-75 -0.06927761982547764 1107 76 1.3465056404182718 1108 1 -0.3760732822416202 1108 2 0.18711797612657222 1108 3 -0.0525687360692757 1108 4 0.4125597677109383 1108 5 0.18592509604108898 1108 6 -0.48806620418625357 1108 7 -0.03264589703561427 1108 8 -0.5093418037806572 1108 9 -0.17860381484922527 1108 10-11 0.1424689008607487 1108 12-13 0.7787433278780895 1108 14-15 0.2854721708386023 1108 16-17 -0.486669874181878 1108 18-19 0.004046571212704464 1108 20-21 0.7966378911375998 1108 22-23 0.10610439345285982 1108 24-25 0.6918595747281984 1108 26-27 0.0058443511271590864 1108 28-29 -0.5848633166806678 1108 30-31 -0.2925159192208113 1108 32-33 0.2589781124973527 1108 34-35 0.5545562396271109 1108 36-37 1.228929701777652 1108 38-39 0.700543277769107 1108 40-41 0.9071642415883332 1108 42-43 -0.44697114814012906 1108 44-45 0.7375534537550621 1108 46-47 0.42661345378969884 1108 48-49 0.779915935481327 1108 50-51 0.5665056085194777 1108 52-53 -0.8212885837660409 1108 54-55 -1.0049997408807698 1108 56-57 0.1746634196830854 1108 58-59 -0.204603623787321 1108 60-61 0.7906122875790302 1108 62-63 0.40903247590707537 1108 64-65 0.180561764881908 1108 66-67 0.7133556620452524 1108 68-69 1.0807644922580337 1108 70-71 -0.9584975915387659 1108 72-73 -0.6148111017544835 1108 74-75 -1.141204500786266 1108 76 -0.01100937194485141 1109 1 -0.08982707464760864 1109 2 0.4786965363913467 1109 3 0.4624170136779 1109 4 -0.1707352584911419 1109 5 0.002878300049545146 1109 6 -0.41892949369623267 1109 7 -0.5025364918687529 1109 8 -0.0634008274085005 1109 9 0.6111819716727922 1109 10-11 0.21719079108783035 1109 12-13 0.32751596739265665 1109 14-15 1.0025586836638212 1109 16-17 0.8650585435803748 1109 18-19 0.9032375246005842 1109 20-21 1.0369155412249498 1109 22-23 0.25364047877049956 1109 24-25 -0.06432319625645277 1109 26-27 0.5184397358839874 1109 28-29 -1.149839872716342 1109 30-31 0.5338148705190022 1109 32-33 0.3866098124146049 1109 34-35 -0.686663765889115 1109 36-37 1.0614432625021166 1109 38-39 0.8264281725011102 1109 40-41 0.3887078657494101 1109 42-43 0.545329118477504 1109 44-45 0.7909919018565645 1109 46-47 0.8517076893325921 1109 48-49 0.7733194474791176 1109 50-51 0.33146837398790296 1109 52-53 -0.25523176662895963 1109 54-55 -0.35160266448102817 1109 56-57 -0.12273016310628293 1109 58-59 0.018228958726240307 1109 60-61 0.5518975684469112 1109 62-63 0.15250494074112453 1109 64-65 0.7402289525255554 1109 66-67 0.22951361000884418 1109 68-69 -0.5781717953212606 1109 70-71 0.09084965494464114 1109 72-73 -0.7189528687858342 1109 74-75 -1.1475941302796997 1109 76 -0.29417568618591616 1110 1 -1.029332774427285 1110 2 -0.46923908962646976 1110 3 -0.0988915407813451 1110 4 0.004712581340264421 1110 5 0.07089247765276951 1110 6 -0.06017865549333834 1110 7 0.5043607205079113 1110 8 0.869782427692563 1110 9 0.9236161128740576 1110 10-11 -0.6525045820322006 1110 12-13 0.6895238225511946 1110 14-15 0.3301523229012986 1110 16-17 0.11965668516481998 1110 18-19 0.14784907743131726 1110 20-21 0.8502118339732903 1110 22-23 -0.23948625750692543 1110 24-25 -0.38370244714255364 1110 26-27 0.8182768850362052 1110 28-29 0.43630343602971067 1110 30-31 0.04167002905909811 1110 32-33 0.8133029822584632 1110 34-35 0.8871699346569173 1110 36-37 1.2400762733269062 1110 38-39 1.0485040418372549 1110 40-41 -0.9312222041806564 1110 42-43 0.6352084172609125 1110 44-45 0.4375816135147659 1110 46-47 0.6582744102828713 1110 48-49 1.0251874424978027 1110 50-51 -0.822521471782764 1110 52-53 0.6003099519264552 1110 54-55 -0.0833777387218575 1110 56-57 0.6967688310502496 1110 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-1.2019034919165037 2116 36-37 0.9757697465203776 2116 38-39 -3.5216882658550546 2116 40-41 -0.8696254675839228 2116 42-43 -0.9972507901982937 2116 44-45 -0.07561768718453266 2116 46-47 -0.1946393426308859 2116 48-49 0.17177835158870636 2116 50-51 -0.003348977610272641 2116 52-53 0.2639463155628121 2116 54-55 -0.39532412566824604 2116 56-57 -0.26884888456747547 2116 58-59 -2.0324559727806104 2116 60-61 -1.025271381324778 2116 62-63 0.5058725206498025 2116 64-65 -1.6190747004424821 2116 66-67 -0.522997805516269 2116 68-69 -1.96766951221624 2116 70-71 1.085769746268845 2116 72-73 0.22425489377124563 2116 74-75 0.21559080122715102 2116 76 -0.9135820788799762 2117 1 0.6750487815167716 2117 2 0.0620655432406636 2117 3 -0.01104189043169157 2117 4 0.09125104287872432 2117 5 0.31816083429612263 2117 6 0.5326972186325349 2117 7 0.5827588848435781 2117 8 0.2074681794408164 2117 9 -1.0289079255874825 2117 10-11 -0.36639225685737387 2117 12-13 -0.9529313784977518 2117 14-15 -0.25857145332247455 2117 16-17 -0.9595882886114069 2117 18-19 -1.332100660330923 2117 20-21 -0.3356764964462897 2117 22-23 -0.032318425339092016 2117 24-25 -1.51375598735234 2117 26-27 -2.479526873705055 2117 28-29 -0.49819175627798273 2117 30-31 -0.8744685198919555 2117 32-33 -2.3977437834758035 2117 34-35 0.6559090080834906 2117 36-37 -1.3856885868129538 2117 38-39 -0.2826257658550517 2117 40-41 -0.6073858842505899 2117 42-43 0.014988793135039202 2117 44-45 -0.5545239371845341 2117 46-47 -0.48005600929754877 2117 48-49 -0.4407216484112908 2117 50-51 -0.2905885609436041 2117 52-53 0.510821315562815 2117 54-55 -0.6200637090015775 2117 56-57 0.17906778209919594 2117 58-59 -1.4840184727806047 2117 60-61 -0.8185005479914409 2117 62-63 -0.7212108126835304 2117 64-65 -1.1985017837758107 2117 66-67 -1.1615394721829375 2117 68-69 0.35863257111709146 2117 70-71 -0.47464692039782364 2117 72-73 0.7568069771045742 2117 74-75 0.6710595512271524 2117 76 0.6812095877866895 2118 1 -0.1413276497809406 2118 2 0.2514143027826492 2118 3 -0.9218243331797851 2118 4 0.19144188257337902 2118 5 0.38173430757856863 2118 6 0.12692431786917524 2118 7 0.8862536367519738 2118 8 0.5719123588301329 2118 9 -1.3705706164271803 2118 10-11 -0.6861083828115753 2118 12-13 -0.9103800903298094 2118 14-15 0.42034076041798585 2118 16-17 -2.348394347771709 2118 18-19 -0.2387681221629805 2118 20-21 -0.5126504945378869 2118 22-23 0.646116689164721 2118 24-25 0.5818129534873577 2118 26-27 -2.0816857477508535 2118 28-29 -0.8666912791787453 2118 30-31 -0.4004107908843224 2118 32-33 0.19642367835625407 2118 34-35 -0.4439836445882648 2118 36-37 -2.91013992269081 2118 38-39 0.458368986053344 2118 40-41 -0.3154906075330359 2118 42-43 0.9328382683258809 2118 44-45 -2.324949748253239 2118 46-47 0.14327414337420663 2118 48-49 0.3006905653291696 2118 50-51 -0.2761861277374962 2118 52-53 -1.4253666615364224 2118 54-55 -0.7196879933527285 2118 56-57 0.10201625538164194 2118 58-59 -0.9893262017882378 2118 60-61 -0.5995716357776999 2118 62-63 -1.520113484439257 2118 64-65 0.15207669904862087 2118 66-67 0.008427130870501287 2118 68-69 -1.3980766368981747 2118 70-71 -0.26591600436729124 2118 72-73 1.3796302118373944 2118 74-75 0.8046175187844042 2118 76 1.2557563435118837 2119 1 0.7493003571470211 2119 2 -0.6388929651626967 2119 3 0.17330684906410454 2119 4 -0.6177825705666535 2119 5 -0.30355660267866824 2119 6 -0.5473973191825934 2119 7 -0.5960094975093639 2119 8 -0.23141049702977057 2119 9 -0.7829126524782382 2119 10-11 0.3113257053274978 2119 12-13 -0.24892008648095043 2119 14-15 -0.2191806970199508 2119 16-17 0.2278132344978374 2119 18-19 -0.19915685780991055 2119 20-21 -1.6251657296395692 2119 22-23 -0.6678226270197669 2119 24-25 0.49415498953841563 2119 26-27 0.13516587839578165 2119 28-29 0.07172683615899444 2119 30-31 1.3834752826290497 2119 32-33 0.4341233383729346 2119 34-35 0.7505124744700495 2119 36-37 0.035398598060993436 2119 38-39 -1.2591882658550517 2119 40-41 -0.6217963359312648 2119 42-43 0.3325111145636086 2119 44-45 0.6915041615549598 2119 46-47 0.05802172179488707 2119 48-49 -0.2337888753020465 2119 50-51 0.6492420588042904 2119 52-53 0.20344211388214006 2119 54-55 -0.143993593455356 2119 56-57 -1.0328544868083682 2119 58-59 -0.5043047122764079 2119 60-61 0.5173056494875468 2119 62-63 0.16087952345092305 2119 64-65 0.6479070922746075 2119 66-67 0.13428510764899215 2119 68-69 0.8702856698565853 2119 70-71 0.4074434157366298 2119 72-73 0.9898361262642368 2119 74-75 -0.4338839886888124 2119 76 0.5316560163581201 >>END_MODULE >>Per sequence quality scores pass #Quality Count 21 1.0 22 7.0 23 4.0 24 20.0 25 45.0 26 78.0 27 146.0 28 276.0 29 451.0 30 794.0 31 1231.0 32 1948.0 33 2871.0 34 4501.0 35 6874.0 36 11899.0 37 19583.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 35.6344497230381 24.199176013719963 11.819669222732559 28.346705040509374 2 17.34510832068442 18.83538015730647 40.20579944410495 23.613712077904157 3 16.164324153837057 26.194090165388634 31.08478385144592 26.556801829328393 4 12.470184706972343 20.20540519229632 38.99938890969663 28.325021191034715 5 12.411046935677819 37.944765321610916 37.353387608665656 12.290800134045615 6 26.5765144197599 40.339845059039206 18.05081905813243 15.032821463068464 7 25.90628634508861 30.067614185180076 24.26422756214394 19.761871907587373 8 21.731159691695083 37.948707839697214 21.029391472333376 19.29074099627432 9 22.937570226103414 15.32062528336849 22.71876047231367 39.02304401821443 10-11 24.20213290228469 26.55778745884997 27.928798123361393 21.311281515503953 12-13 23.907429675333635 22.93165644897396 26.869246387667804 26.2916674880246 14-15 21.636539257623845 25.07047251079264 23.68664866250074 29.606339569082774 16-17 22.72467424944312 28.003705967001125 25.397701511955685 23.87391827160007 18-19 22.378718287370145 27.675491336316504 26.07384336375643 23.87194701255692 20-21 21.612884149106034 27.12452443375584 25.90628634508861 25.35630507204952 22-23 22.620197520156125 28.05791559068777 26.42571310295886 22.896173786197245 24-25 22.662579589583867 28.511305170612474 25.874746200398196 22.951369039405467 26-27 21.57247333872144 28.408799700368625 28.07565692207613 21.943070038833802 28-29 21.649352441404325 26.705631887086284 28.65914959884878 22.985866072660606 30-31 22.41420095014686 26.946125490350685 28.344733781466225 22.294939778036234 32-33 21.250172485166274 27.91795619862406 27.655778745884994 23.176092570324666 34-35 21.615841037670762 28.834591653689213 27.75335606852096 21.796211240119064 36-37 22.490094423308165 27.524690019515464 26.914585345660274 23.070630211516097 38-39 21.53403378738 26.918527863746576 27.80460880364289 23.74282954523054 40-41 20.643024699875813 26.08862780658006 29.351061522994737 23.91728597054939 42-43 22.350135031244456 25.4371266928187 29.241656646099862 22.971081629836977 44-45 20.001379881330205 26.81602239350273 29.345147745865287 23.83744997930178 46-47 22.08795757850539 25.36616136726527 28.781367659524136 23.764513394705197 48-49 21.064874135110095 25.49823572315638 29.10465414260088 24.332235999132646 50-51 19.81509590175245 25.297167300754992 29.952295531155748 24.93544126633681 52-53 19.38141891225926 26.183248240651302 31.23558516824696 23.199747678842478 54-55 18.75751542510201 26.27688304520097 30.290366457056123 24.6752350726409 56-57 20.03292002602062 26.134952394094107 28.977507934317646 24.854619645567624 58-59 18.804825642137633 27.070314810069192 29.81627865717834 24.308580890614838 60-61 20.038833803150073 28.283624751128546 27.701117703877465 23.976423741843917 62-63 18.44704212580575 29.42005558950502 27.076228587198642 25.056673697490588 64-65 19.1271264956928 29.388515444814605 25.68451970273414 25.799838356758464 66-67 19.02166413688423 29.608310828125923 25.555402235407755 25.814622799582093 68-69 19.099528869088687 29.554101204439277 25.55145971732145 25.794910209150583 70-71 19.380433282737684 28.745884996747424 25.35334818348479 26.5203335370301 72-73 19.507579491020916 27.978079599440164 25.35531944252794 27.15902146701098 74-75 19.322281140964733 27.965266415659684 26.19113327682391 26.521319166551677 76 19.70273413629285 27.790809990340833 26.724358847996214 25.782097025370103 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.5 10 0.5 11 0.0 12 0.0 13 2.5 14 4.0 15 1.5 16 1.0 17 2.0 18 1.0 19 2.5 20 9.0 21 13.0 22 18.0 23 26.0 24 37.0 25 45.0 26 109.0 27 174.5 28 216.5 29 257.0 30 313.5 31 439.0 32 658.5 33 809.0 34 902.0 35 1163.0 36 1417.5 37 1504.0 38 1758.5 39 2365.0 40 3173.5 41 3734.0 42 3838.0 43 3799.5 44 3828.0 45 3843.5 46 3792.0 47 3807.0 48 3631.0 49 3259.5 50 3079.0 51 2780.5 52 2216.5 53 1762.0 54 1573.0 55 1501.0 56 1209.0 57 815.5 58 642.0 59 562.0 60 393.0 61 261.5 62 219.0 63 173.0 64 111.5 65 86.5 66 71.5 67 66.0 68 48.0 69 20.0 70 10.0 71 10.0 72 6.5 73 2.0 74 1.0 75 1.0 76 1.0 77 0.5 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 50729.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 43.69295669143882 #Duplication Level Percentage of deduplicated Percentage of total 1 59.733814572524246 26.09946973131739 2 16.17866004962779 14.137869857477972 3 8.563049853372434 11.224348991701 4 4.899616512519738 8.563149283447338 5 3.099481163997293 6.771274813223206 6 2.174599593954433 5.700881152792289 7 1.3986013986013985 4.277632123637367 8 0.9023234829686443 3.1540144690413765 9 0.6406496729077374 2.5192690571467997 >10 2.3911572298669075 14.973683691773937 >50 0.009023234829686443 0.3272290011630428 >100 0.004511617414843221 0.8318713162096631 >500 0.004511617414843221 1.4193065110686196 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGT 720 1.4193065110686196 No Hit ATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGTCTT 422 0.8318713162096631 Illumina PCR Primer Index 9 (95% over 21bp) ATAAAGAATATTGAGGCGCCATTGGCGTGAAGGTAGCGGATGATTCAGCC 92 0.18135583196987914 No Hit CTCTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCC 74 0.14587316919316368 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0019712590431508604 0.0 2 0.0 0.0 0.0 0.003942518086301721 0.0 3 0.0 0.0 0.0 0.003942518086301721 0.0 4 0.0 0.0 0.0 0.003942518086301721 0.0 5 0.0 0.0 0.0 0.005913777129452582 0.0 6 0.0 0.0 0.0 0.005913777129452582 0.0 7 0.0 0.0 0.0 0.005913777129452582 0.0 8 0.0 0.0 0.0 0.005913777129452582 0.0 9 0.0 0.0 0.0 0.017741331388357744 0.0 10 0.0 0.0 0.0 0.021683849474659466 0.0 11 0.0 0.0 0.0 0.023655108517810327 0.0 12 0.0 0.0 0.0 0.023655108517810327 0.0 13 0.0 0.0 0.0 0.023655108517810327 0.0 14 0.0 0.0 0.0 0.023655108517810327 0.0 15 0.0 0.0 0.0 0.03351140373356463 0.0 16 0.0 0.0 0.0 0.03548266277671549 0.0 17 0.0 0.0 0.0 0.03942518086301721 0.0 18 0.0 0.0 0.0 0.04336769894931893 0.0 19 0.0 0.0 0.0 0.04533895799246979 0.0 20 0.0 0.0 0.0 0.05322399416507323 0.0 21 0.0 0.0 0.0 0.05519525320822409 0.0 22 0.0 0.0 0.0 0.06308028938082753 0.0 23 0.0 0.0 0.0 0.0650515484239784 0.0 24 0.0 0.0 0.0 0.06702280746712926 0.0 25 0.0 0.0 0.0 0.0749078436397327 0.0 26 0.0 0.0 0.0 0.07885036172603442 0.0 27 0.0 0.0 0.0 0.08673539789863786 0.0 28 0.0 0.0 0.0 0.10053421120069388 0.0 29 0.0 0.0 0.0 0.13798813302056023 0.0 30 0.0 0.0 0.0 0.17347079579727573 0.0 31 0.0 0.0 0.0 0.19121212718563346 0.0 32 0.0 0.0 0.0 0.25034989848015926 0.0 33 0.0 0.0 0.0 0.29568885647262905 0.0 34 0.0 0.0 0.0 0.34102781446509883 0.0 35 0.0 0.0 0.0 0.4553608389678488 0.0 36 0.0 0.0 0.0 0.6308028938082754 0.0 37 0.0 0.0 0.0 0.946204340712413 0.0 38 0.0 0.0 0.0 1.3483411855151886 0.0 39 0.0 0.0 0.0 1.8332709101303002 0.0 40 0.0 0.0 0.0 2.414792327859804 0.0 41 0.0 0.0 0.0 3.1717558004297346 0.0 42 0.0 0.0 0.0 3.968144453862682 0.0 43 0.0 0.0 0.0 4.752705553036725 0.0 44 0.0 0.0 0.0 5.533324134124466 0.0 45 0.0 0.0 0.0 6.442074553017012 0.0 46 0.0 0.0 0.0 7.279859646356128 0.0 47 0.0 0.0 0.0 8.168897474817166 0.0 48 0.0 0.0 0.0 8.973171164422716 0.0 49 0.0 0.0 0.0 9.718307082733743 0.0 50 0.0 0.0 0.0 10.485126850519427 0.0 51 0.0 0.0 0.0 11.29728557629758 0.0 52 0.0 0.0 0.0 12.001025054702438 0.0 53 0.0 0.0 0.0 12.756017268229218 0.0 54 0.0 0.0 0.0 13.37893512586489 0.0 55 0.0 0.0 0.0 14.023536832975221 0.0 56 0.0 0.0 0.0 14.543949220367049 0.0 57 0.0 0.0 0.0 15.155039523743815 0.0 58 0.0 0.0 0.0 15.821325080328807 0.0 59 0.0 0.0 0.0 16.45015671509393 0.0 60 0.0 0.0 0.0 17.00408050621932 0.0 61 0.0 0.0 0.0 17.465355122316623 0.0 62 0.0 0.0 0.0 18.072502907607088 0.0 63 0.0 0.0 0.0 18.703305801415365 0.0 64 0.0 0.0 0.0 19.35776380374145 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTCAATA 15 0.0022126248 70.0 53 GTACATG 20 7.855863E-5 70.0 1 TTCACGC 15 0.0022126248 70.0 37 CACGCCA 15 0.0022126248 70.0 39 GCGCCTC 15 0.0022126248 70.0 49 AATGGCG 15 0.0022126248 70.0 45 TACATGC 20 7.855863E-5 70.0 2 CTTTATC 15 0.0022126248 70.0 62 GCCAATG 15 0.0022126248 70.0 42 CGCCAAT 15 0.0022126248 70.0 41 CTTGTAC 25 2.3692285E-4 55.999996 3 ATCCTAC 25 2.3692285E-4 55.999996 55 AACAAGT 25 2.3692285E-4 55.999996 70 CGGAACA 25 2.3692285E-4 55.999996 67 GCGAACT 25 2.3692285E-4 55.999996 28 ACGGAAC 25 2.3692285E-4 55.999996 66 TACCTTC 25 2.3692285E-4 55.999996 33 AGAATAT 25 2.3692285E-4 55.999996 5 AAAGCGA 25 2.3692285E-4 55.999996 25 CAAAGCG 25 2.3692285E-4 55.999996 24 >>END_MODULE