FastQCFastQC Report
Wed 25 May 2016
SRR1780556_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780556_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences117926
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGGCG150.002220902669.9923622
CCGCGGA150.002220902669.9923636
GGTCTAA150.002220902669.9923621
TTGCTAA252.3835003E-455.99389327
CCGGCAC200.006732371652.898081
TCTGCCA200.00693964252.49427413
AGTCCCC200.00693964252.49427444
CAAGTTG200.00693964252.4942745
ACGCAGG200.00693964252.49427470
GGTGCAA200.00693964252.49427411
GCTCGCG200.00693964252.49427429
TGTGTCC200.00693964252.49427439
GGCTCGC200.00693964252.49427428
GTTGCCA200.00693964252.49427466
CGCCAAG200.00693964252.49427436
GATTTCC305.8609934E-446.66157560
CGGGGTC305.8609934E-446.66157527
ACGATGT305.8609934E-446.6615758
TACGATG305.8609934E-446.6615757
CGAGCGC305.8609934E-446.66157544
Produced by FastQC (version 0.11.5)