##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780556_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 117926 Sequences flagged as poor quality 0 Sequence length 76 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.60146193375507 34.0 31.0 34.0 12.0 34.0 2 30.95891491274189 34.0 32.0 34.0 23.0 34.0 3 31.350083950952293 34.0 33.0 34.0 23.0 34.0 4 31.73189966589217 34.0 34.0 34.0 23.0 34.0 5 31.33863609382155 34.0 33.0 34.0 23.0 34.0 6 34.49698963756932 38.0 35.0 38.0 26.0 38.0 7 33.62222919457965 37.0 34.0 38.0 21.0 38.0 8 32.61764157183318 37.0 32.0 38.0 11.0 38.0 9 33.45029934026423 37.0 34.0 38.0 21.0 38.0 10-11 33.86338466495938 37.0 34.0 38.0 22.0 38.0 12-13 34.24892305344029 38.0 34.5 38.0 24.0 38.0 14-15 33.820641758390856 37.0 34.0 38.0 22.0 38.0 16-17 33.68472601461934 37.0 34.0 38.0 21.0 38.0 18-19 33.66335244136153 37.0 34.0 38.0 22.0 38.0 20-21 33.76033699099435 37.0 34.0 38.0 21.5 38.0 22-23 33.75082678968167 37.0 34.0 38.0 22.0 38.0 24-25 34.20567983311568 37.5 34.5 38.0 23.5 38.0 26-27 34.314209758662216 38.0 34.5 38.0 23.5 38.0 28-29 34.461997354273024 38.0 35.0 38.0 24.5 38.0 30-31 34.27446025473602 38.0 34.5 38.0 24.0 38.0 32-33 34.006949273273065 37.0 34.0 38.0 23.0 38.0 34-35 33.86455489035497 37.0 34.0 38.0 23.0 38.0 36-37 33.82605617081899 37.0 34.0 38.0 22.5 38.0 38-39 34.453424181266215 37.5 35.0 38.0 25.0 38.0 40-41 33.85510828824856 37.0 34.0 38.0 22.5 38.0 42-43 34.23631345080813 37.0 34.0 38.0 24.0 38.0 44-45 33.199960144497396 37.0 34.0 38.0 21.0 38.0 46-47 33.9595254651222 37.0 34.0 38.0 23.0 38.0 48-49 33.64177535064363 37.0 34.0 38.0 22.5 38.0 50-51 33.578846056001225 37.0 34.0 38.0 22.0 38.0 52-53 33.59192205281278 37.0 34.0 38.0 22.0 38.0 54-55 33.29816155894375 37.0 33.5 38.0 21.0 38.0 56-57 33.442608076251204 37.0 33.5 38.0 22.0 38.0 58-59 33.28251615419839 37.0 33.0 38.0 22.0 38.0 60-61 33.29606702508353 37.0 33.5 38.0 21.5 38.0 62-63 33.40643708766514 37.0 33.0 38.0 22.0 38.0 64-65 33.42319335854688 37.0 33.0 38.0 21.5 38.0 66-67 33.3610187744857 37.0 33.5 38.0 21.5 38.0 68-69 32.9035327239116 37.0 31.5 38.0 16.5 38.0 70-71 32.72217746722521 37.0 31.5 38.0 19.5 38.0 72-73 32.86910435357767 37.0 32.0 38.0 20.0 38.0 74-75 32.43428506012245 37.0 31.0 38.0 15.0 38.0 76 31.561453793056664 36.0 28.0 37.0 11.0 38.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -2.7819793145765708 1101 2 -1.693340037772348 1101 3 -0.4993273590563341 1101 4 -3.4233368991614483 1101 5 -1.5866724028042896 1101 6 -3.131823191599622 1101 7 -1.2491265600764123 1101 8 -0.6111915650217696 1101 9 -1.4218348476280873 1101 10-11 -0.8720707438126141 1101 12-13 -1.7873827159256024 1101 14-15 -0.8661246710444672 1101 16-17 -2.027266326591409 1101 18-19 -0.1811308989672611 1101 20-21 -1.5976971503281803 1101 22-23 -0.47057954093485677 1101 24-25 -1.9453519844514844 1101 26-27 -1.6134629570362264 1101 28-29 -0.9144811977477971 1101 30-31 -1.5850233070037163 1101 32-33 -0.18208514344336635 1101 34-35 -1.4386945071801165 1101 36-37 -0.5009535423560507 1101 38-39 -1.0995380946489348 1101 40-41 -0.5842562713618662 1101 42-43 -1.405415496615504 1101 44-45 -0.09750517533789349 1101 46-47 -0.21855338505498878 1101 48-49 -1.6608785435742242 1101 50-51 -0.8862352328405478 1101 52-53 -0.7734029658401766 1101 54-55 -2.0893921945530565 1101 56-57 -1.2954832056125838 1101 58-59 -0.8641030771651188 1101 60-61 -0.6605574089555475 1101 62-63 -1.8344942771583206 1101 64-65 -0.8546389236002341 1101 66-67 -0.8926657060010896 1101 68-69 0.08241896261309734 1101 70-71 0.22530927253363586 1101 72-73 -0.6626986462686446 1101 74-75 -1.873214369197573 1101 76 -1.7654434572533368 1102 1 0.7215337545003742 1102 2 0.5227798320543542 1102 3 0.4994077060878084 1102 4 0.737401822794375 1102 5 0.893393373808216 1102 6 1.1477159121365332 1102 7 1.8375444763652098 1102 8 2.8468528456910818 1102 9 1.6522443372938938 1102 10-11 0.28304476773854503 1102 12-13 -3.325865484063044 1102 14-15 -0.3749102185887665 1102 16-17 -1.9151815989548844 1102 18-19 -0.33053695455540577 1102 20-21 0.8965071481504481 1102 22-23 -0.28479510883311576 1102 24-25 1.213845816861749 1102 26-27 -5.124858870146703 1102 28-29 0.5824944580059324 1102 30-31 0.9967202332039591 1102 32-33 -6.508145101355538 1102 34-35 -0.13632332929577728 1102 36-37 1.1073536298245799 1102 38-39 1.3052468089285298 1102 40-41 1.542208465704178 1102 42-43 1.075179252754907 1102 44-45 -5.20321348136013 1102 46-47 0.7330053596696544 1102 48-49 1.1255199569029983 1102 50-51 -5.644776417969517 1102 52-53 -1.0082266224218657 1102 54-55 1.6847824354534993 1102 56-57 -0.5718082248740899 1102 58-59 1.5229612390225995 1102 60-61 1.0180973903222892 1102 62-63 -4.581490056875666 1102 64-65 -0.35657082410735086 1102 66-67 0.780061148565899 1102 68-69 -8.115681335221563 1102 70-71 0.4852188871884806 1102 72-73 1.1359582229025946 1102 74-75 -6.573994029045089 1102 76 -0.9467201215051269 1103 1 -0.681580808452928 1103 2 -0.5683843741866283 1103 3 -0.3384018233681303 1103 4 0.3354730071583205 1103 5 0.15913911005848647 1103 6 -1.7411202768715839 1103 7 -0.34170099309191926 1103 8 0.8354795526731564 1103 9 -0.14845130113222638 1103 10-11 0.37792925618738593 1103 12-13 -1.5421606165703068 1103 14-15 -0.608848283053895 1103 16-17 -0.9656647909866649 1103 18-19 -0.21241675930234294 1103 20-21 -0.849668816751425 1103 22-23 -0.31456230710983135 1103 24-25 0.7880192521693488 1103 26-27 -0.8476113849191336 1103 28-29 0.4140985765016225 1103 30-31 -0.12252148138666286 1103 32-33 -4.746628530536782 1103 34-35 -0.8841346373726964 1103 36-37 -0.028666409305081686 1103 38-39 0.26049966954393255 1103 40-41 0.3969346568861667 1103 42-43 0.36762900671233467 1103 44-45 -5.617133166741844 1103 46-47 -0.22254887838887782 1103 48-49 0.1888783885545493 1103 50-51 -0.2063858723281271 1103 52-53 -0.2875541060684341 1103 54-55 0.0030518372439729546 1103 56-57 0.94223299960764 1103 58-59 0.4017432789032327 1103 60-61 0.8260112655421494 1103 62-63 0.4172896115103413 1103 64-65 0.6192547524622825 1103 66-67 -0.03950269404590756 1103 68-69 -3.0616952479900874 1103 70-71 -0.004598507724665524 1103 72-73 0.9019855741407099 1103 74-75 -1.765299536841379 1103 76 0.3151027673705862 1104 1 -1.8665985281156452 1104 2 0.009852737595640093 1104 3 0.33932726655697465 1104 4 0.3768479831538514 1104 5 -0.24087136461897884 1104 6 -0.39727153121421566 1104 7 1.437213481166566 1104 8 1.4901381654767007 1104 9 -0.6942518283576575 1104 10-11 -0.6796509187397248 1104 12-13 -0.6097179217849558 1104 14-15 -0.43410447620694725 1104 16-17 -1.024741971901797 1104 18-19 0.8664051886385096 1104 20-21 1.1963368395743998 1104 22-23 -0.014240210777707318 1104 24-25 0.36756133418607106 1104 26-27 0.47666717167031436 1104 28-29 -0.4865141921302083 1104 30-31 -1.333014489539444 1104 32-33 -0.5232996780376951 1104 34-35 0.02752891598706242 1104 36-37 0.23843350166016108 1104 38-39 0.18187412180559193 1104 40-41 0.7535450512577739 1104 42-43 0.8224875112775507 1104 44-45 -3.6787111769022793 1104 46-47 0.4720531711563467 1104 48-49 0.9279716306417995 1104 50-51 1.413522997977907 1104 52-53 -0.28005161833253567 1104 54-55 -0.7613683020692292 1104 56-57 0.29987340393019224 1104 58-59 1.015581200727219 1104 60-61 0.6731551701527536 1104 62-63 0.5840508412374703 1104 64-65 0.4105249816831389 1104 66-67 0.5458964784545728 1104 68-69 -3.8536899757357617 1104 70-71 -0.3967486098527715 1104 72-73 0.9072010906295986 1104 74-75 -0.7461788860147536 1104 76 -0.1947259716528258 1105 1 -1.638991635214392 1105 2 -0.20035386460061133 1105 3 -0.6778209677882217 1105 4 -0.24714274117211232 1105 5 0.1963960083656211 1105 6 -0.4679145269880678 1105 7 0.7544180080987886 1105 8 0.4852146645819033 1105 9 -0.12883060017103531 1105 10-11 0.6509840112306122 1105 12-13 -2.5297488406984208 1105 14-15 -0.7839418998548737 1105 16-17 0.6084866180109074 1105 18-19 0.2161669953784937 1105 20-21 -0.9653783199352617 1105 22-23 -0.11439060440572035 1105 24-25 0.06297222806194469 1105 26-27 -0.20193156300676662 1105 28-29 -1.4006091837703494 1105 30-31 -0.44019305466661507 1105 32-33 0.5056363920675793 1105 34-35 0.11878172011405752 1105 36-37 0.23662289680891746 1105 38-39 0.48925685258419094 1105 40-41 0.16450614215770543 1105 42-43 0.4355809989814077 1105 44-45 1.7613246151008113 1105 46-47 0.8047423604590804 1105 48-49 0.08997245402590437 1105 50-51 -0.8906082057592428 1105 52-53 -1.5448536280619116 1105 54-55 -1.4974442886598354 1105 56-57 -0.24965987171024295 1105 58-59 -0.254803857371769 1105 60-61 0.05268903392469326 1105 62-63 -0.14111950685236252 1105 64-65 -0.13762198397838432 1105 66-67 0.17833109083390042 1105 68-69 1.2629911141559447 1105 70-71 1.7060651793104853 1105 72-73 0.2104248914714688 1105 74-75 -1.1243264398077812 1105 76 -0.6789178369539073 1106 1 -0.5061890177768547 1106 2 0.27525507957910733 1106 3 -0.7382160562884259 1106 4 -0.36490840822872883 1106 5 0.12771680615145087 1106 6 0.24672581312663766 1106 7 0.9959603179493683 1106 8 1.4541135717636848 1106 9 1.2760067135315154 1106 10-11 0.4249589591576779 1106 12-13 0.03222032451781587 1106 14-15 0.02773004543763591 1106 16-17 0.45610552975798413 1106 18-19 0.047350834223472305 1106 20-21 -0.29986248881325395 1106 22-23 0.14589796047381753 1106 24-25 0.6362880610861694 1106 26-27 -0.16611299555924575 1106 28-29 -0.9967134627861398 1106 30-31 -0.6550949483141935 1106 32-33 1.2806337765322482 1106 34-35 1.2960199050276557 1106 36-37 0.8532282172833234 1106 38-39 0.26069235348298037 1106 40-41 -0.0848542405664574 1106 42-43 0.5817799128209131 1106 44-45 1.192826122600266 1106 46-47 1.0052825873924363 1106 48-49 0.8449919040977178 1106 50-51 0.6654546051327941 1106 52-53 -0.7111969194515666 1106 54-55 0.47851180839079177 1106 56-57 1.0602049764460446 1106 58-59 -0.16515757285859678 1106 60-61 0.9124868292661859 1106 62-63 0.8970577979098167 1106 64-65 1.022967129688027 1106 66-67 1.2371568581368493 1106 68-69 1.7259028231942821 1106 70-71 0.7178921545152122 1106 72-73 0.9824928763679353 1106 74-75 -1.3594725768998757 1106 76 -0.8048059300859869 1107 1 -1.086612849633724 1107 2 -0.3790787748589679 1107 3 0.44382320026880606 1107 4 0.1959450455376981 1107 5 0.08096213068390412 1107 6 0.39842519359099526 1107 7 0.165232508326298 1107 8 0.6390478546656624 1107 9 -0.9115041428172788 1107 10-11 -1.0518953052161208 1107 12-13 -0.2060258685225449 1107 14-15 -0.21963548058865712 1107 16-17 0.28722416793759464 1107 18-19 0.8997623911686432 1107 20-21 0.7223713135143512 1107 22-23 -0.5078424135635871 1107 24-25 -0.3983798793920883 1107 26-27 0.5124667045160294 1107 28-29 -1.3303073484905 1107 30-31 -0.4355795757242973 1107 32-33 0.7573722398522662 1107 34-35 -7.347704398910082E-4 1107 36-37 -0.26439670310345065 1107 38-39 0.5797821572001993 1107 40-41 0.6982793359490955 1107 42-43 0.41478031812459903 1107 44-45 0.5506803290735434 1107 46-47 0.22319122036099515 1107 48-49 0.08607522295591963 1107 50-51 0.8444685591597789 1107 52-53 1.1665026926148983 1107 54-55 0.8452971711375952 1107 56-57 0.7652886427074108 1107 58-59 0.8089464164877072 1107 60-61 -0.5959955979238956 1107 62-63 -0.28281018888885967 1107 64-65 0.02982834212505736 1107 66-67 1.1493651842326216 1107 68-69 1.010353636696685 1107 70-71 -0.09314947596362799 1107 72-73 -0.01766635008102213 1107 74-75 0.027737196709061607 1107 76 -1.3343351988023322 1108 1 -1.062877039520206 1108 2 0.6185476441296807 1108 3 0.21054146652267747 1108 4 0.3074173537592344 1108 5 0.22736735944207354 1108 6 0.5803309383450426 1108 7 1.3047061925368268 1108 8 1.789495051088089 1108 9 0.41780442271419815 1108 10-11 0.8735174914815076 1108 12-13 0.989364568353956 1108 14-15 1.2395578458647307 1108 16-17 -0.06275003226880216 1108 18-19 0.5567713350970962 1108 20-21 0.8366643991696705 1108 22-23 -0.012580975655090754 1108 24-25 -0.4864259750233302 1108 26-27 -0.5697142380364255 1108 28-29 0.8922158713237351 1108 30-31 0.8545391033262675 1108 32-33 0.8530808741831848 1108 34-35 0.2981214387104387 1108 36-37 0.5504811778735004 1108 38-39 1.6105613109669719 1108 40-41 0.8000079221204004 1108 42-43 1.3921710989983538 1108 44-45 0.6090996617188793 1108 46-47 1.1284285784621275 1108 48-49 0.6844842356838186 1108 50-51 0.7742587208384819 1108 52-53 0.35866815529119833 1108 54-55 0.8840398611960723 1108 56-57 1.3278617421226144 1108 58-59 -0.7520872952425961 1108 60-61 1.7036002053096766 1108 62-63 0.541699615032698 1108 64-65 0.977893357604934 1108 66-67 0.3125402788318681 1108 68-69 1.9228063370750803 1108 70-71 2.553229953000944 1108 72-73 1.1074540489557876 1108 74-75 1.464226704792999 1108 76 1.204570889948961 1109 1 -0.05901835318853088 1109 2 -0.9289227726084412 1109 3 -0.08049780632932269 1109 4 -0.16834152121932888 1109 5 0.17312280447723083 1109 6 -0.3506243008935144 1109 7 -2.011766110670173 1109 8 0.5863480780131454 1109 9 0.43111260312922184 1109 10-11 0.9879875652252821 1109 12-13 -0.025169816828686464 1109 14-15 -0.3014514149824592 1109 16-17 -0.983925645371194 1109 18-19 -0.24438198337315242 1109 20-21 0.47038931770851633 1109 22-23 0.3880921507383235 1109 24-25 0.4244126461394231 1109 26-27 0.42418903755952186 1109 28-29 -0.017126437028174735 1109 30-31 0.1013878428220707 1109 32-33 1.096233849659093 1109 34-35 0.055225708989979694 1109 36-37 0.18303991565432653 1109 38-39 0.5127779118930533 1109 40-41 0.011562543969148464 1109 42-43 0.004703254717782102 1109 44-45 -0.7618015559110276 1109 46-47 -0.62707219327514 1109 48-49 0.06645559565637882 1109 50-51 0.8654180417096882 1109 52-53 0.856974620734519 1109 54-55 -0.013554890990519652 1109 56-57 -0.20158432201733234 1109 58-59 0.32607682754937883 1109 60-61 0.7956041497445909 1109 62-63 -0.39262554360217905 1109 64-65 -0.1361222486375624 1109 66-67 0.12169822772570882 1109 68-69 1.2439776627482004 1109 70-71 0.34669162338337856 1109 72-73 -0.5884257315278418 1109 74-75 1.6590689273963264 1109 76 -0.40463850809597446 1110 1 -0.18420056893197057 1110 2 0.658804997070856 1110 3 0.27359293711091226 1110 4 0.561277647876878 1110 5 0.6545732417950134 1110 6 0.1869485854038615 1110 7 0.8203311925368268 1110 8 2.066149462852792 1110 9 -0.48146028316815404 1110 10-11 -0.8736332438126126 1110 12-13 0.26041236247160526 1110 14-15 0.5049990223353262 1110 16-17 0.6640882030253152 1110 18-19 1.2019919233323932 1110 20-21 -0.013703247889161219 1110 22-23 0.7258381419919715 1110 24-25 0.8725813779178537 1110 26-27 -1.2011480615658456 1110 28-29 0.6548077830884438 1110 30-31 0.7973699856792109 1110 32-33 1.7212794035949557 1110 34-35 1.110897174004549 1110 36-37 0.43761353081468 1110 38-39 0.9457635168493255 1110 40-41 0.8716071868262887 1110 42-43 1.2567850695865843 1110 44-45 1.8912687793659373 1110 46-47 0.7765903431680101 1110 48-49 1.173730559213233 1110 50-51 1.0676410737796616 1110 52-53 0.5999365376441403 1110 54-55 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50-51 0.8847162371783526 2116 52-53 1.5980472402585235 2116 54-55 -0.013835955797389943 2116 56-57 -0.1188702840211775 2116 58-59 -1.0212049423014165 2116 60-61 -2.0615958731217 2116 62-63 1.0194773928104865 2116 64-65 1.6496253837487274 2116 66-67 0.10894550759002897 2116 68-69 0.26267561812083073 2116 70-71 -3.026672007783368 2116 72-73 0.29846712085120686 2116 74-75 -1.865021661220073 2116 76 -1.7718996982863366 2117 1 -0.4078847794582856 2117 2 -0.6203068450344063 2117 3 0.26948109500564854 2117 4 -0.7181631415968042 2117 5 -1.051347810836564 2117 6 -0.9729198356487672 2117 7 -3.2163464390421233 2117 8 -3.335001852936678 2117 9 -0.40711817790499794 2117 10-11 -0.7536496911810389 2117 12-13 0.08582354668213554 2117 14-15 -1.1262509776646752 2117 16-17 -0.6595960075010012 2117 18-19 -0.12070544508866732 2117 20-21 -0.4870585110470458 2117 22-23 -0.5707079106396051 2117 24-25 0.16485111475996206 2117 26-27 0.3828979910657324 2117 28-29 0.041403177825287685 2117 30-31 -0.38897869853131795 2117 32-33 0.3619044035949557 2117 34-35 -0.317803483890188 2117 36-37 -2.071596995501114 2117 38-39 -0.3451081936769924 2117 40-41 -1.8258434710684526 2117 42-43 -1.5771787462028826 2117 44-45 0.08649904252384033 2117 46-47 -1.3464359726214639 2117 48-49 -0.7322727302604548 2117 50-51 0.3212594948322902 2117 52-53 0.7352161429072979 2117 54-55 -1.4810917177512977 2117 56-57 -1.4329935210352822 2117 58-59 -1.5751910104128726 2117 60-61 0.33784943131586687 2117 62-63 -1.2455294871344762 2117 64-65 -1.348461131559148 2117 66-67 -2.0969798450071444 2117 68-69 -0.5983817434202763 2117 70-71 0.1226765474281919 2117 72-73 0.3763394978721948 2117 74-75 -1.1656107565073128 2117 76 -0.7148176549426815 2118 1 0.9260701317049715 2118 2 0.13271025184792862 2118 3 0.22592175876696174 2118 4 0.09915583259025595 2118 5 -0.13595223591199002 2118 6 0.41393903126373743 2118 7 1.1840055555941476 2118 8 -4.188567893835103 2118 9 -1.4226227035503207 2118 10-11 -1.0520070495450966 2118 12-13 0.5314215185225635 2118 14-15 -0.9481060732060769 2118 16-17 -0.1853146632167224 2118 18-19 -1.0401657199797185 2118 20-21 -3.4383449676343787 2118 22-23 -0.6067454567341457 2118 24-25 -0.29360094692291483 2118 26-27 0.39723967728765786 2118 28-29 -0.45702541754850046 2118 30-31 -0.9643537404354419 2118 32-33 -0.32519750723306373 2118 34-35 0.40549311349499817 2118 36-37 -0.2984135392490188 2118 38-39 -0.8007631551251961 2118 40-41 -1.4474911411991869 2118 42-43 -0.861079182005767 2118 44-45 -0.01436418241749493 2118 46-47 -0.6400895931377235 2118 48-49 -1.4491894248632065 2118 50-51 -0.12272516825855462 2118 52-53 -1.081243796750762 2118 54-55 -0.22503657192494586 2118 56-57 0.6390082389378975 2118 58-59 0.5907283660537743 2118 60-61 -1.8839700457206732 2118 62-63 0.4326887495400058 2118 64-65 -0.4772115506003871 2118 66-67 -2.628617458148277 2118 68-69 1.0637767379742868 2118 70-71 -1.0445313808319554 2118 72-73 -1.9903634857013905 2118 74-75 -0.027390005585417043 2118 76 -0.5598352546744962 2119 1 1.120836744500867 2119 2 0.9498031313992144 2119 3 0.6763448027825518 2119 4 0.3837123493694179 2119 5 1.34878965970546 2119 6 1.1572844062993823 2119 7 -1.5676818671646657 2119 8 0.9995916270318972 2119 9 -0.16261699958606357 2119 10-11 0.12046656962021984 2119 12-13 0.5496147878447388 2119 14-15 0.08130499248458278 2119 16-17 -0.46343045369110314 2119 18-19 -0.5305640468168704 2119 20-21 -0.6141696657996008 2119 22-23 0.9357728434845072 2119 24-25 -0.07233466685826784 2119 26-27 0.37501798321027024 2119 28-29 0.814625880103371 2119 30-31 0.4811852841866724 2119 32-33 0.4869044035949557 2119 34-35 0.7216947486314211 2119 36-37 -0.2768454244092027 2119 38-39 -0.040733684643214474 2119 40-41 -0.12543105944236999 2119 42-43 -0.7808315348910213 2119 44-45 -0.4682927877982408 2119 46-47 -0.36865219414542594 2119 48-49 -0.8598748512345296 2119 50-51 1.237230626018473 2119 52-53 -0.9997602907140646 2119 54-55 -0.647676556714373 2119 56-57 1.3256229361524632 2119 58-59 1.6772147240725914 2119 60-61 0.5753297926670058 2119 62-63 -0.29276043764508586 2119 64-65 0.5994473523371582 2119 66-67 1.161464774003072 2119 68-69 0.023991587294567296 2119 70-71 -0.61571210143277 2119 72-73 -1.4373879176816153 2119 74-75 0.20698350900722318 2119 76 1.4658088179559812 >>END_MODULE >>Per sequence quality scores pass #Quality Count 12 24.0 13 102.0 14 297.0 15 549.0 16 733.0 17 860.0 18 883.0 19 900.0 20 887.0 21 930.0 22 959.0 23 1046.0 24 1225.0 25 1540.0 26 1793.0 27 2154.0 28 2608.0 29 3220.0 30 3860.0 31 4749.0 32 6136.0 33 7951.0 34 10601.0 35 15219.0 36 24221.0 37 24479.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 36.4883331625158 21.572546206142597 10.781148577089953 31.157972054251648 2 17.92649627732646 19.420653630242697 35.66897885114394 26.98387124128691 3 18.122381832674726 23.79543103302071 29.32262605362685 28.759561080677713 4 14.245374217729761 17.63224394959551 37.47858826721843 30.6437935654563 5 13.20658718179197 35.405254142428305 34.98295541271645 16.405203263063278 6 28.931702932347402 37.74061699709988 16.902973050896325 16.424707019656395 7 26.778657802350626 30.87868663399081 22.387768600647863 19.9548869630107 8 23.12551939351797 34.48433763546631 20.46792056035141 21.922222410664315 9 23.671624578125265 16.142326543764735 21.905262622322475 38.28078625578753 10-11 24.95717653443685 25.55840103115513 27.0381425639808 22.446279870427215 12-13 24.054491799942337 23.5126265624205 26.254600342587725 26.178281295049437 14-15 21.547411088309616 25.256092803961806 24.012092329087732 29.184403778640842 16-17 22.103268151213474 27.595695605718838 26.19015314688873 24.11088309617896 18-19 22.00532537353934 26.62220375489714 26.61329986601767 24.759171005545852 20-21 21.540203178264335 27.26243576480166 26.359751030307145 24.83761002662687 22-23 22.670149076539523 26.818513304953957 26.670115156962844 23.84122246154368 24-25 22.148211590319352 27.51004867459254 26.553940606821225 23.78779912826688 26-27 21.07338500415515 28.011634414802504 27.002527008462934 23.912453572579416 28-29 21.982853653986396 26.782049760018996 28.27493512880959 22.960161457185013 30-31 22.22665061140037 27.0593422994081 27.129301426318197 23.58470566287333 32-33 21.159031935281448 27.764869494428712 27.076726082458492 23.99937248783135 34-35 22.185523124671406 27.16491698183607 27.396418092702202 23.253141800790324 36-37 22.485287383613453 27.128877431609656 26.853704865763273 23.532130319013618 38-39 21.91416651120194 27.323914997540832 27.68346251038787 23.078455980869357 40-41 22.081644421077627 26.097298305717143 27.885283991655786 23.935773281549448 42-43 21.35788545358954 26.312687617658533 27.96287502331971 24.36655190543222 44-45 20.658718179197123 25.864949205433916 28.589963197259298 24.88636941810966 46-47 20.954666485762257 25.501161745501417 29.357393619727624 24.1867781490087 48-49 20.033325984091718 26.22322473415532 29.516815630140936 24.22663365161203 50-51 20.2118277563896 26.440734019639432 28.857079863643303 24.49035836032766 52-53 19.135729186099756 26.2681681732612 29.46000033919577 25.13610230144328 54-55 19.003442837033393 26.777385818224992 29.11741261469057 25.10175873005105 56-57 19.3294947679053 26.66036327866628 28.834608144090364 25.175533809338056 58-59 19.273103471668673 26.388158675779728 28.344894255719687 25.993843596831912 60-61 19.488492783610063 26.082458490918032 28.65610637179248 25.772942353679422 62-63 19.543188101012497 26.2304326442006 28.589963197259298 25.6364160575276 64-65 20.29620270339026 26.2995437816936 27.397690076827843 26.006563438088293 66-67 20.59935892000068 26.17997727388362 26.71929854315418 26.501365262961517 68-69 19.94555907942269 25.899716771534692 26.75109814629513 27.403626002747483 70-71 21.13444024218578 25.801773993860554 26.75067415158659 26.31311161236708 72-73 21.299374183810187 25.922188491087635 25.795838067940913 26.982599257161272 74-75 21.169207808286554 26.447517934976172 25.54907314756712 26.83420110917016 76 21.7526245272459 25.806861930363105 25.6152163221003 26.82529722029069 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 1.0 14 3.0 15 4.0 16 10.0 17 16.0 18 19.0 19 27.0 20 34.0 21 36.0 22 45.5 23 81.0 24 117.5 25 128.0 26 190.5 27 314.0 28 520.0 29 665.0 30 729.5 31 963.0 32 1382.0 33 1632.0 34 1866.5 35 2453.0 36 3110.5 37 3416.0 38 3865.5 39 4820.0 40 5885.0 41 6797.0 42 7149.0 43 7390.5 44 8024.5 45 8526.0 46 8635.0 47 8636.0 48 8334.0 49 7725.5 50 7420.0 51 6977.0 52 6005.0 53 5026.5 54 4577.0 55 4292.5 56 3519.5 57 2680.5 58 2330.0 59 1993.0 60 1501.0 61 1170.0 62 994.0 63 835.0 64 606.5 65 456.0 66 315.0 67 255.0 68 208.0 69 133.0 70 91.5 71 78.0 72 66.5 73 40.5 74 22.0 75 18.0 76 18.5 77 20.0 78 17.0 79 13.0 80 12.5 81 7.5 82 5.5 83 8.0 84 6.0 85 3.0 86 1.5 87 1.0 88 1.5 89 1.0 90 1.5 91 2.5 92 2.0 93 2.5 94 2.5 95 1.0 96 0.0 97 0.5 98 0.5 99 3.5 100 7.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.7140070891915269 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 117926.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.25137798280277 #Duplication Level Percentage of deduplicated Percentage of total 1 76.41818044772745 42.98627953123145 2 10.05954624255672 11.31726676051083 3 4.876761890404763 8.229737292878585 4 2.6079746740031657 5.868086766277157 5 1.4758423155197105 4.150908196665705 6 0.9828898771387654 3.3173345996641963 7 0.7009874123765735 2.7602055526347034 8 0.512549935931258 2.3065312144904433 9 0.38290495213688097 1.9385038074724827 >10 1.977839752770031 16.98353204552007 >50 0.004522499434687571 0.14161423265437648 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0033919576683682988 0.0 2 0.0 0.0 0.0 0.0033919576683682988 0.0 3 0.0 0.0 0.0 0.0033919576683682988 0.0 4 0.0 0.0 0.0 0.0033919576683682988 0.0 5 0.0 0.0 0.0 0.0033919576683682988 0.0 6 0.0 0.0 0.0 0.005935925919644523 0.0 7 0.0 0.0 0.0 0.0067839153367365975 0.0 8 0.0 0.0 0.0 0.0067839153367365975 0.0 9 0.0 0.0 0.0 0.01271984125638112 0.0 10 0.0 0.0 0.0 0.027983650764038463 0.0 11 0.0 0.0 0.0 0.03137560843240676 0.0 12 0.0 0.0 0.0 0.033919576683682985 0.0 13 0.0 0.0 0.0 0.036463544934959215 0.0 14 0.0 0.0 0.0 0.039855502603327514 0.0 15 0.0 0.0 0.0 0.04155148143751166 0.0 16 0.0 0.0 0.0 0.04155148143751166 0.0 17 0.0 0.0 0.0 0.045791428522972036 0.0 18 0.0 0.0 0.0 0.049183386191340335 0.0 19 0.0 0.0 0.0 0.058511269779353156 0.0 20 0.0 0.0 0.0 0.06529518511608975 0.0 21 0.0 0.0 0.0 0.07207910045282635 0.0 22 0.0 0.0 0.0 0.07547105812119465 0.0 23 0.0 0.0 0.0 0.07631904753828672 0.0 24 0.0 0.0 0.0 0.08140698404083917 0.0 25 0.0 0.0 0.0 0.08564693112629955 0.0 26 0.0 0.0 0.0 0.08903888879466784 0.0 27 0.0 0.0 0.0 0.12465444431253497 0.0 28 0.0 0.0 0.0 0.15348608449366552 0.0 29 0.0 0.0 0.0 0.18316571409188814 0.0 30 0.0 0.0 0.0 0.2238692061123077 0.0 31 0.0 0.0 0.0 0.26966063463527973 0.0 32 0.0 0.0 0.0 0.3129080949069756 0.0 33 0.0 0.0 0.0 0.3620914810983159 0.0 34 0.0 0.0 0.0 0.4350185709682343 0.0 35 0.0 0.0 0.0 0.5664569306175059 0.0 36 0.0 0.0 0.0 0.7564065600461306 0.0 37 0.0 0.0 0.0 1.0752505808727506 0.0 38 0.0 0.0 0.0 1.5280769295999186 0.0 39 0.0 0.0 0.0 2.130149415735292 0.0 40 0.0 0.0 0.0 2.7483337007954143 0.0 41 0.0 0.0 0.0 3.5242440174346625 0.0 42 0.0 0.0 0.0 4.490103963502535 0.0 43 0.0 0.0 0.0 5.455963909570409 0.0 44 0.0 0.0 0.0 6.3785763953665855 0.0 45 0.0 0.0 0.0 7.317300680087513 0.0 46 0.0 0.0 0.0 8.18733782202398 0.0 47 0.0 0.0 0.0 9.136237979750012 0.0 48 0.0 0.0 0.0 9.889252582127774 0.0 49 0.0 0.0 0.0 10.584603904143277 0.0 50 0.0 0.0 0.0 11.258755490731476 0.0 51 0.0 0.0 0.0 12.030425860285265 0.0 52 0.0 0.0 0.0 13.009005647609518 0.0 53 0.0 0.0 0.0 13.87225887420925 0.0 54 0.0 0.0 0.0 14.791479402337059 0.0 55 0.0 0.0 0.0 15.611485168665094 0.0 56 0.0 0.0 0.0 16.325492257856624 0.0 57 0.0 0.0 0.0 16.95809236300731 0.0 58 0.0 0.0 0.0 17.625460034258772 0.0 59 0.0 0.0 0.0 18.210572732052302 0.0 60 0.0 0.0 0.0 18.799925376931295 0.0 61 0.0 0.0 0.0 19.396061937147024 0.0 62 0.0 0.0 0.0 19.993046486779846 0.0 63 0.0 0.0 0.0 20.667198073368045 0.0 64 0.0 0.0 0.0 21.358309448298087 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCCGGCG 15 0.0022209026 69.99236 22 CCGCGGA 15 0.0022209026 69.99236 36 GGTCTAA 15 0.0022209026 69.99236 21 TTGCTAA 25 2.3835003E-4 55.993893 27 CCGGCAC 20 0.0067323716 52.89808 1 TCTGCCA 20 0.006939642 52.494274 13 AGTCCCC 20 0.006939642 52.494274 44 CAAGTTG 20 0.006939642 52.494274 5 ACGCAGG 20 0.006939642 52.494274 70 GGTGCAA 20 0.006939642 52.494274 11 GCTCGCG 20 0.006939642 52.494274 29 TGTGTCC 20 0.006939642 52.494274 39 GGCTCGC 20 0.006939642 52.494274 28 GTTGCCA 20 0.006939642 52.494274 66 CGCCAAG 20 0.006939642 52.494274 36 GATTTCC 30 5.8609934E-4 46.661575 60 CGGGGTC 30 5.8609934E-4 46.661575 27 ACGATGT 30 5.8609934E-4 46.661575 8 TACGATG 30 5.8609934E-4 46.661575 7 CGAGCGC 30 5.8609934E-4 46.661575 44 >>END_MODULE