FastQCFastQC Report
Wed 25 May 2016
SRR1780556_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780556_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences117926
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGT6530.5537370893611249No Hit
ATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGTCTT3870.3281719044146329RNA PCR Primer, Index 30 (95% over 22bp)
ATAAAGAATATTGAGGCGCCATTGGCGTGAAGGTAGCGGATGATTCAGCC2340.19842952359954547No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTGTAC150.002219942270.010
CTGTACC150.002219942270.02
GTACTCG150.002219942270.013
GACGGTT150.002219942270.011
TACTCGT150.002219942270.014
CGTTTAA150.002219942270.055
ACGGTTT150.002219942270.012
AAATGCG252.3822158E-456.0000045
CTTCGAG200.006936650352.531
ACGCCTT200.006936650352.510
TACTTCG200.006936650352.529
CCCAGAT200.006936650352.529
ACTTCGA200.006936650352.530
TTCCGGC200.006936650352.541
CGCTCGT200.006936650352.512
ACGGCAT200.006936650352.557
TGCGAAT200.006936650352.530
CGGTGTA200.006936650352.59
GACGGCA200.006936650352.556
TCGTAGG200.006936650352.517
Produced by FastQC (version 0.11.5)