##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780556_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 117926 Sequences flagged as poor quality 0 Sequence length 76 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.88914234350355 34.0 34.0 34.0 31.0 34.0 2 33.335931007581024 34.0 34.0 34.0 33.0 34.0 3 33.46447772331801 34.0 34.0 34.0 34.0 34.0 4 33.490485558740225 34.0 34.0 34.0 34.0 34.0 5 33.50347675661008 34.0 34.0 34.0 34.0 34.0 6 37.22831267065787 38.0 37.0 38.0 36.0 38.0 7 36.82191374251649 38.0 37.0 38.0 35.0 38.0 8 36.8255855366925 38.0 37.0 38.0 35.0 38.0 9 36.6178705289758 38.0 37.0 38.0 34.0 38.0 10-11 36.34786221867951 38.0 37.0 38.0 33.5 38.0 12-13 36.219290063260004 38.0 37.0 38.0 34.0 38.0 14-15 36.46858199209674 38.0 37.0 38.0 34.0 38.0 16-17 36.62174160066482 38.0 37.0 38.0 34.0 38.0 18-19 36.63674253345318 38.0 37.0 38.0 34.0 38.0 20-21 36.357542017875616 38.0 37.0 38.0 34.0 38.0 22-23 36.41375099638756 38.0 37.0 38.0 34.0 38.0 24-25 35.75287468412394 38.0 36.5 38.0 31.5 38.0 26-27 35.95807540321897 38.0 37.0 38.0 33.0 38.0 28-29 35.80500907348676 38.0 36.5 38.0 31.5 38.0 30-31 35.53325814493835 38.0 36.0 38.0 31.0 38.0 32-33 36.038384240964675 38.0 37.0 38.0 33.5 38.0 34-35 36.10732154062717 38.0 37.0 38.0 33.0 38.0 36-37 36.26087546427421 38.0 37.0 38.0 34.0 38.0 38-39 35.92201465325713 38.0 36.5 38.0 32.5 38.0 40-41 35.405690856978104 38.0 36.0 38.0 31.0 38.0 42-43 35.81055916422163 38.0 36.5 38.0 32.5 38.0 44-45 36.01579804284043 38.0 37.0 38.0 33.0 38.0 46-47 36.3101012499364 38.0 37.0 38.0 34.0 38.0 48-49 36.099558197513694 38.0 37.0 38.0 33.5 38.0 50-51 36.26022251242304 38.0 37.0 38.0 34.0 38.0 52-53 36.19120465376592 38.0 37.0 38.0 34.0 38.0 54-55 36.35779217475366 38.0 37.0 38.0 34.0 38.0 56-57 36.41456506622797 38.0 37.0 38.0 34.0 38.0 58-59 36.47871546563099 38.0 37.0 38.0 34.0 38.0 60-61 36.168932211726 38.0 37.0 38.0 33.0 38.0 62-63 36.43532384715839 38.0 37.0 38.0 34.0 38.0 64-65 36.43391194477893 38.0 37.0 38.0 34.0 38.0 66-67 35.85038922714244 38.0 37.0 38.0 31.5 38.0 68-69 35.29504519783593 38.0 36.0 38.0 29.0 38.0 70-71 35.373785255160016 38.0 36.0 38.0 31.0 38.0 72-73 35.202817868832994 38.0 36.0 38.0 29.0 38.0 74-75 35.84764598137815 38.0 36.5 38.0 32.0 38.0 76 35.29564303037498 38.0 36.0 38.0 31.0 38.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.5168909416591418 1101 2 0.4656621413591111 1101 3 -0.04991549954154806 1101 4 0.31128913173041894 1101 5 0.37315427430552006 1101 6 -0.8409912403932012 1101 7 0.13456218752675397 1101 8 0.5566140995214681 1101 9 0.10887474522993301 1101 10-11 0.0517300165898007 1101 12-13 0.7369465357751608 1101 14-15 0.5652218475863222 1101 16-17 -0.04417865396153786 1101 18-19 -0.5722219826174211 1101 20-21 -0.029006006478169866 1101 22-23 0.5252867344747969 1101 24-25 -1.2830359009845935 1101 26-27 -0.25363361647949034 1101 28-29 0.802030368947598 1101 30-31 1.1293275795627409 1101 32-33 0.16651315517509602 1101 34-35 0.19168783343885565 1101 36-37 -0.17564433034657867 1101 38-39 0.05974610381404233 1101 40-41 1.1504338672729872 1101 42-43 0.261364973439278 1101 44-45 0.27605750504319104 1101 46-47 0.3546105929622314 1101 48-49 -1.78317085834248 1101 50-51 -1.788924766642367 1101 52-53 -1.8126975029210612 1101 54-55 -1.0987738925573538 1101 56-57 -1.4894134438143567 1101 58-59 -1.3728980132922999 1101 60-61 -1.9133559864004965 1101 62-63 -0.7356750970833232 1101 64-65 -2.0019893171110184 1101 66-67 -1.385299538049935 1101 68-69 -1.2504287287651437 1101 70-71 -1.0752954856292476 1101 72-73 -2.029754717584858 1101 74-75 -1.0467103650965441 1101 76 -0.8623612494789796 1102 1 -0.5262663357410702 1102 2 0.13639959838007343 1102 3 0.23934060376913635 1102 4 0.4105290208761332 1102 5 0.16984474474629252 1102 6 0.23025909021477275 1102 7 -0.022381729861500332 1102 8 0.554164725664215 1102 9 0.5960931534819025 1102 10-11 0.6462666479532899 1102 12-13 0.6293293280613597 1102 14-15 0.07805518666935995 1102 16-17 0.339746323902105 1102 18-19 -0.35532590276552867 1102 20-21 0.3383713235893353 1102 22-23 0.7326670543883225 1102 24-25 -4.552559020543935 1102 26-27 -0.7301058281605819 1102 28-29 0.24398135847162905 1102 30-31 1.6368136940278433 1102 32-33 0.5118288714157089 1102 34-35 0.6921880576773063 1102 36-37 -2.2064569934944274 1102 38-39 -0.045576972047548736 1102 40-41 0.6884681585069856 1102 42-43 0.902175221711488 1102 44-45 0.8737449741385319 1102 46-47 1.1873689559065355 1102 48-49 -2.851063361878545 1102 50-51 -0.4150560208827727 1102 52-53 -0.14967614852080402 1102 54-55 0.5809676007557343 1102 56-57 0.7068871967026524 1102 58-59 0.1343293644977379 1102 60-61 -5.703647036411418 1102 62-63 -2.2966679559510865 1102 64-65 -0.013431227852613858 1102 66-67 1.056685949697446 1102 68-69 -0.6782756946464019 1102 70-71 -0.8452383335725742 1102 72-73 -5.973948091626095 1102 74-75 -1.7982311617755116 1102 76 -0.28818431959086865 1103 1 0.8137467074794245 1103 2 0.48811201513073144 1103 3 0.06784201678468804 1103 4 0.4069618976967462 1103 5 0.21759083672075974 1103 6 0.2672939314234455 1103 7 0.7432750753399731 1103 8 0.6498248904830959 1103 9 0.24537874072326815 1103 10-11 -0.07751845510792066 1103 12-13 -0.6309570299157627 1103 14-15 -0.3100102665825446 1103 16-17 0.31528534487006965 1103 18-19 0.5766023199968657 1103 20-21 -0.08358674048054837 1103 22-23 0.47575513565153216 1103 24-25 -3.0016509357234753 1103 26-27 -0.29535157429208425 1103 28-29 -0.3391889346055734 1103 30-31 0.9136856926049433 1103 32-33 1.1067528847751262 1103 34-35 0.4753000515114252 1103 36-37 -0.8686277015832005 1103 38-39 -0.2435671303448217 1103 40-41 -0.12760761074658689 1103 42-43 -0.4571457403248331 1103 44-45 0.9048313163056818 1103 46-47 0.7366861630763566 1103 48-49 -2.4730767608232327 1103 50-51 -0.7237554537003064 1103 52-53 0.13061969543483798 1103 54-55 -0.17938792581488627 1103 56-57 0.37380089406040895 1103 58-59 -0.40238433681250996 1103 60-61 -3.9346948417907583 1103 62-63 -1.041924966682103 1103 64-65 0.22856003714215234 1103 66-67 -0.4925571482650426 1103 68-69 0.42341695964896786 1103 70-71 0.5276693164738688 1103 72-73 -4.774189193580604 1103 74-75 -2.3620935360368875 1103 76 -3.0409065978067105 1104 1 0.16916997173807857 1104 2 0.22168859375191374 1104 3 0.37502939130678214 1104 4 0.09625677732524451 1104 5 -0.24961469390894564 1104 6 0.009040758469062382 1104 7 0.596839084348197 1104 8 0.7166795194176458 1104 9 0.9082774331343586 1104 10-11 -0.7448994365852002 1104 12-13 0.17899304078831335 1104 14-15 0.3964456262964191 1104 16-17 0.10859813619703829 1104 18-19 0.5335164800150238 1104 20-21 -0.28016152094877356 1104 22-23 0.16209964779343267 1104 24-25 0.02504203402216376 1104 26-27 0.7957868485706427 1104 28-29 0.9432520144002012 1104 30-31 0.7690914990678266 1104 32-33 0.10151991049046671 1104 34-35 0.005916660858325429 1104 36-37 0.9892920274718122 1104 38-39 1.0110722788833684 1104 40-41 1.3445602983332137 1104 42-43 0.2083890792488461 1104 44-45 0.6859629306280581 1104 46-47 0.7741156772259075 1104 48-49 -0.3531417038235247 1104 50-51 0.5463166469962601 1104 52-53 0.3655361598820406 1104 54-55 -0.0321095961767881 1104 56-57 -0.1528920756852301 1104 58-59 -0.8294151149064675 1104 60-61 0.18911148142287004 1104 62-63 0.04367497046983004 1104 64-65 0.3139461162958028 1104 66-67 -0.2949916378863904 1104 68-69 1.5156595880947066 1104 70-71 1.3750707235722004 1104 72-73 1.0378339903721923 1104 74-75 0.31192577228524243 1104 76 -0.38884919657419204 1105 1 0.6846504331692529 1105 2 0.3622772293708394 1105 3 0.2589238301032921 1105 4 -0.13464365201075168 1105 5 -0.5058383855263884 1105 6 -0.08606850796290644 1105 7 0.5803041871451811 1105 8 -1.1077464222229025 1105 9 0.37790044083888574 1105 10-11 0.062298452862890485 1105 12-13 -0.5603302719044052 1105 14-15 0.059694128661540447 1105 16-17 -0.032334976446954045 1105 18-19 0.43519349499557336 1105 20-21 0.30833546697773073 1105 22-23 0.210116384335727 1105 24-25 0.4597720396514191 1105 26-27 0.31764433590480934 1105 28-29 0.5600759362291257 1105 30-31 0.9787687829084888 1105 32-33 1.014106764911105 1105 34-35 -0.9475243285469048 1105 36-37 0.2547997950086298 1105 38-39 -0.2008399046808691 1105 40-41 0.49003462639296913 1105 42-43 0.14824002992141772 1105 44-45 0.6355600605463039 1105 46-47 0.7158402468396687 1105 48-49 -0.4593750576014344 1105 50-51 0.6125402545949186 1105 52-53 0.40087026058696296 1105 54-55 0.6681029713533135 1105 56-57 0.39652777459329513 1105 58-59 0.16337607320319592 1105 60-61 0.6418509139599848 1105 62-63 -0.4123411947447053 1105 64-65 0.6543482302084698 1105 66-67 0.49029415201624005 1105 68-69 0.26501474390754254 1105 70-71 0.9647691801470941 1105 72-73 0.7171048753420735 1105 74-75 -0.35842651848697926 1105 76 0.9262800663289923 1106 1 -1.4206557746849633 1106 2 -0.13422746432620158 1106 3 0.2591425839671544 1106 4 0.18280624859890082 1106 5 0.04113187345916458 1106 6 0.22035810011576729 1106 7 -0.9596754592344396 1106 8 0.3462439335850078 1106 9 -0.285094965329975 1106 10-11 0.5604580670951975 1106 12-13 1.2992963247610234 1106 14-15 -0.2239250113504383 1106 16-17 0.25063741301101317 1106 18-19 0.6760272325479946 1106 20-21 0.4868861750744813 1106 22-23 0.6930630939922793 1106 24-25 -0.4436481294548287 1106 26-27 0.8144486272849534 1106 28-29 0.6911760779435809 1106 30-31 0.6879688095393988 1106 32-33 0.036581346663233205 1106 34-35 0.9628151203835813 1106 36-37 1.1747311253174573 1106 38-39 0.36696428207786624 1106 40-41 1.1604153532264618 1106 42-43 1.1381488190712332 1106 44-45 1.2367812777688911 1106 46-47 0.8903392529362435 1106 48-49 1.218243568814522 1106 50-51 -0.0635708723679187 1106 52-53 0.6110499240864513 1106 54-55 -0.021342630267362495 1106 56-57 0.2728937973627197 1106 58-59 0.6491808496462568 1106 60-61 0.8491582441166372 1106 62-63 0.8369954103855477 1106 64-65 -1.201550039733796 1106 66-67 0.44117439854233 1106 68-69 1.240866219545012 1106 70-71 0.2603722274835292 1106 72-73 -0.08285898271520864 1106 74-75 -1.0540067393332606 1106 76 -0.4532008212410332 1107 1 0.6809596500154029 1107 2 0.03032583310881165 1107 3 -0.008531551867484666 1107 4 0.2820188014331322 1107 5 0.022980058277646265 1107 6 0.2553879188871164 1107 7 0.7209162841504266 1107 8 -0.11221243836797612 1107 9 -0.47034770112987445 1107 10-11 0.40305548473170205 1107 12-13 -0.08011135284884574 1107 14-15 0.05094326495087387 1107 16-17 0.5420073394773439 1107 18-19 0.6743191670046045 1107 20-21 0.6045545208564249 1107 22-23 0.027843940497980668 1107 24-25 0.08874721534280638 1107 26-27 -0.13629249945929445 1107 28-29 0.6559436073280978 1107 30-31 0.6910520125965434 1107 32-33 0.9248622800197239 1107 34-35 -0.08739911156592939 1107 36-37 1.0396347209401782 1107 38-39 0.477105559363487 1107 40-41 0.36869802359876047 1107 42-43 0.7185292255329259 1107 44-45 0.5620519627321272 1107 46-47 0.6426986994072053 1107 48-49 0.6372552068204271 1107 50-51 0.7517409219694073 1107 52-53 0.36424892819739085 1107 54-55 0.7025587072348074 1107 56-57 0.8067945558596179 1107 58-59 0.6212438454195208 1107 60-61 0.8465671955380927 1107 62-63 0.6268164977574386 1107 64-65 0.9110809602083023 1107 66-67 -0.011361644535661242 1107 68-69 1.0865468402386682 1107 70-71 0.3318504279351515 1107 72-73 1.1685306299302738 1107 74-75 -0.20078457501156777 1107 76 0.7951055883472975 1108 1 -0.718782352048585 1108 2 0.15598802781124732 1108 3 -0.05301039603673274 1108 4 0.5038798265449316 1108 5 0.03180358768941005 1108 6 -0.1210998692105818 1108 7 0.12420145786903447 1108 8 -0.5199179767236686 1108 9 -0.3960442955571182 1108 10-11 0.4030468848005029 1108 12-13 1.0868133117538434 1108 14-15 0.45384144176545504 1108 16-17 -0.28205526802180003 1108 18-19 0.206938706048291 1108 20-21 0.93812865226338 1108 22-23 -0.26362492775107427 1108 24-25 0.7849288458897661 1108 26-27 -0.30513494871970437 1108 28-29 -0.9840374728232604 1108 30-31 0.26594881342213483 1108 32-33 0.7331354138346597 1108 34-35 0.6419696534839474 1108 36-37 1.1294438024675273 1108 38-39 1.1644550605674766 1108 40-41 1.0129876692815927 1108 42-43 0.11692963832962278 1108 44-45 0.929690421624457 1108 46-47 0.8319573853377165 1108 48-49 0.9825854441785253 1108 50-51 0.9632734297093464 1108 52-53 0.22194586662188698 1108 54-55 -0.2916105394112165 1108 56-57 0.3666328771530516 1108 58-59 0.5319851594890039 1108 60-61 1.0598454893117406 1108 62-63 1.022507932225956 1108 64-65 0.5570647923376484 1108 66-67 0.8800212244013821 1108 68-69 1.5163370019173783 1108 70-71 0.027000066738047224 1108 72-73 0.10723032033274649 1108 74-75 -0.761723687498673 1108 76 1.2427320073359454 1109 1 -1.6529874626642567 1109 2 0.15754865206095303 1109 3 0.20010227760415233 1109 4 0.24786027586751658 1109 5 0.1119013410764822 1109 6 -0.2504344601898296 1109 7 -0.06496849582672581 1109 8 0.39306435906779313 1109 9 0.7974902611226966 1109 10-11 0.3673454613739864 1109 12-13 0.5696635947241759 1109 14-15 0.6078158886870852 1109 16-17 0.6669071740979078 1109 18-19 0.6064027773911107 1109 20-21 0.9384973711795155 1109 22-23 0.4594757410878927 1109 24-25 -0.40207166860174226 1109 26-27 -0.3747670872894133 1109 28-29 -1.0159316590691887 1109 30-31 0.17288158653137486 1109 32-33 0.5446685985371076 1109 34-35 -0.07134281436032808 1109 36-37 0.775006313186104 1109 38-39 1.0548040573090276 1109 40-41 0.7133649630562431 1109 42-43 1.202558175458762 1109 44-45 0.6495197819400076 1109 46-47 0.6137658229985021 1109 48-49 0.29700406877122276 1109 50-51 0.12940702600501197 1109 52-53 -0.4916066972608135 1109 54-55 -0.8484361267890321 1109 56-57 0.07013999428561846 1109 58-59 -0.12679120820092749 1109 60-61 0.6439219770496933 1109 62-63 -0.09772444643979128 1109 64-65 0.7570948043424437 1109 66-67 0.11317162741973874 1109 68-69 -0.39833458100150665 1109 70-71 0.1534963795488764 1109 72-73 0.13550162885616146 1109 74-75 -0.1603774347118474 1109 76 0.004736809256712604 1110 1 -0.5941499991074082 1110 2 -0.41662226630639765 1110 3 0.027871956904448325 1110 4 0.24064453242728234 1110 5 0.4479800582776434 1110 6 0.020811895495306487 1110 7 0.4499367519866837 1110 8 0.7539790820998604 1110 9 0.9824483515017093 1110 10-11 -0.6236075269642072 1110 12-13 0.5551956646950202 1110 14-15 0.7941907064713405 1110 16-17 0.5170256143311533 1110 18-19 -0.10225247042230023 1110 20-21 0.9047646816751396 1110 22-23 -0.4505734571628395 1110 24-25 -0.46994247763964836 1110 26-27 1.251298874809713 1110 28-29 0.3246022330590961 1110 30-31 -0.4257791277543319 1110 32-33 0.6356170314817149 1110 34-35 0.5949108299545358 1110 36-37 1.1939658612910549 1110 38-39 0.9980028546851187 1110 40-41 -0.8464792424831131 1110 42-43 0.11077155009432715 1110 44-45 0.8666389510362222 1110 46-47 0.47184709122007007 1110 48-49 1.1623648559432382 1110 50-51 -0.3525353938200624 1110 52-53 0.4838943960336479 1110 54-55 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36-37 0.40607778939563133 2116 38-39 -3.908257357733177 2116 40-41 -0.45465938954193774 2116 42-43 -0.8608481394481515 2116 44-45 -0.20282591824481955 2116 46-47 -0.5721275819825422 2116 48-49 0.25464426770794546 2116 50-51 -0.6646677467612392 2116 52-53 -0.0030541333781215485 2116 54-55 -0.7641595590190633 2116 56-57 -0.2791187568338813 2116 58-59 -2.7374592849554347 2116 60-61 -1.6395009159170115 2116 62-63 0.28247525248739436 2116 64-65 -1.1986541619107172 2116 66-67 -0.3487369455332541 2116 68-69 -1.00882639677544 2116 70-71 0.9276536615092894 2116 72-73 0.6533087517052962 2116 74-75 0.22422402491962856 2116 76 -1.3406013259973903 2117 1 0.8476263166820672 2117 2 0.5690685231672887 2117 3 0.4245824832202345 2117 4 -0.07580283599376969 2117 5 0.13482216354080379 2117 6 0.32410136917951604 2117 7 0.6459893835656274 2117 8 0.26680802946827953 2117 9 -0.832189806393032 2117 10-11 -0.36357463222736186 2117 12-13 -1.3602648616207702 2117 14-15 0.10430583805028704 2117 16-17 -1.0301783330372771 2117 18-19 -0.9861340493696673 2117 20-21 -0.061929397272230347 2117 22-23 0.1795252270476908 2117 24-25 -1.2736431355343854 2117 26-27 -1.9248524409797696 2117 28-29 -1.4995753985198519 2117 30-31 -0.8933778119648608 2117 32-33 -1.8861757316761754 2117 34-35 0.24556872469138114 2117 36-37 -1.1358038755510478 2117 38-39 0.16387456521143662 2117 40-41 -0.20330490037785154 2117 42-43 0.36381431325222735 2117 44-45 -0.4611571015953544 2117 46-47 -0.9770015929904545 2117 48-49 -0.5244772493199221 2117 50-51 -0.19891697276743514 2117 52-53 0.6577430802441739 2117 54-55 -0.25084041557220615 2117 56-57 0.4928325675555243 2117 58-59 -0.9613584937617716 2117 60-61 -0.4403480091402656 2117 62-63 -0.3298720987337944 2117 64-65 -0.6841310280957913 2117 66-67 -0.6188177848865379 2117 68-69 0.34294303906906975 2117 70-71 -0.43075922118764964 2117 72-73 0.5895832615092189 2117 74-75 0.17202244253228827 2117 76 0.37624593922448923 2118 1 0.09888344038304098 2118 2 0.3015106954770417 2118 3 -0.7433063870445977 2118 4 0.2885744687330174 2118 5 0.4592857907617258 2118 6 0.39206985727874155 2118 7 0.9395068156809501 2118 8 0.802824623501138 2118 9 -1.0159393873517928 2118 10-11 -0.4266927180470077 2118 12-13 -0.643610067789055 2118 14-15 0.787552569528664 2118 16-17 -2.6316805958599403 2118 18-19 -0.3534865468554216 2118 20-21 -0.15878030240128993 2118 22-23 0.6380610969772889 2118 24-25 1.0674878567552568 2118 26-27 -2.049318163406852 2118 28-29 -0.2224320026096933 2118 30-31 0.0827996939017197 2118 32-33 0.13785628307407194 2118 34-35 0.3291464987443433 2118 36-37 -2.891921877562446 2118 38-39 -0.5793160147416287 2118 40-41 -0.26404492719648687 2118 42-43 0.6417329354446508 2118 44-45 -2.186486048963779 2118 46-47 0.053116995678678336 2118 48-49 0.4581849196375032 2118 50-51 -0.9253459033742075 2118 52-53 -0.9681159542848192 2118 54-55 -1.0816629935138806 2118 56-57 0.3301398085880436 2118 58-59 -0.9197476992595881 2118 60-61 -0.7546917905683586 2118 62-63 -1.0037846867324518 2118 64-65 0.12567663197796008 2118 66-67 0.20514299285399318 2118 68-69 -1.3328761865427268 2118 70-71 -1.002823837345879 2118 72-73 0.7297106500442538 2118 74-75 -0.38731379615359884 2118 76 -0.02113086265281794 2119 1 0.5997865680567713 2119 2 -0.6979189080974422 2119 3 -0.189665356528387 2119 4 -0.7664450198115205 2119 5 0.037905431411971335 2119 6 -0.6595985522658907 2119 7 -0.6509960838342153 2119 8 0.029818634338667493 2119 9 -0.2884938126773946 2119 10-11 0.03884583124475682 2119 12-13 -0.1811849323199084 2119 14-15 -0.5182393308420927 2119 16-17 -0.3972001319375096 2119 18-19 -0.24227112713871435 2119 20-21 -1.4892418481756025 2119 22-23 -0.29558278552104156 2119 24-25 0.8740640522110965 2119 26-27 0.7478940240634344 2119 28-29 0.14862275544714976 2119 30-31 1.0891928871710377 2119 32-33 0.5613399792429092 2119 34-35 0.5038660538351323 2119 36-37 -0.06932704915670485 2119 38-39 -1.1540260632253236 2119 40-41 -1.076586518602518 2119 42-43 0.0688172590495526 2119 44-45 0.3732154435735353 2119 46-47 0.1273041807723132 2119 48-49 -0.5446314127134855 2119 50-51 0.8397220688665072 2119 52-53 0.13785894827245215 2119 54-55 0.2542410848205634 2119 56-57 -1.259376780441329 2119 58-59 -0.16186909863215249 2119 60-61 0.12470501521166 2119 62-63 -0.2619960186081087 2119 64-65 -0.05915557640687297 2119 66-67 0.26884914362877765 2119 68-69 0.6901298026197438 2119 70-71 -0.3790359297501098 2119 72-73 0.856374306285332 2119 74-75 0.3230436522730571 2119 76 0.03443525579950801 >>END_MODULE >>Per sequence quality scores pass #Quality Count 19 1.0 20 2.0 21 8.0 22 11.0 23 18.0 24 47.0 25 84.0 26 190.0 27 306.0 28 590.0 29 1005.0 30 1562.0 31 2579.0 32 4218.0 33 6402.0 34 10373.0 35 15934.0 36 28301.0 37 46295.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 37.54642742058579 22.963553414853383 12.136424537421773 27.353594627139056 2 18.334379186947746 19.399453894815395 37.42346895510744 24.84269796312942 3 17.71449892305344 24.733307328324543 29.192035683394675 28.36015806522735 4 13.490663636517816 17.683971304038128 38.487695673557994 30.337669385886063 5 13.227786917219273 36.65434255380493 36.239675728846905 13.878194800128895 6 29.656733883961127 38.55468683750827 16.975900140766242 14.812679137764363 7 28.06675372691349 29.682173566473892 22.917761986330408 19.33331072028221 8 24.21264182623001 35.30773535946272 20.349202041958517 20.13042077234876 9 23.80051896952326 15.681020300866647 22.351305055712906 38.16715567389719 10-11 25.01399182538202 25.800926004443465 27.53167240472839 21.653409765446128 12-13 24.49290232857894 23.300205213438936 26.75279412512932 25.454098332852805 14-15 21.80943981819107 25.286196428268575 23.944253175720366 28.960110577819986 16-17 22.650221325237858 27.920475552465106 26.157929549039228 23.271373573257804 18-19 22.367840849346198 27.228516188117975 26.888048437155504 23.515594525380322 20-21 21.579634686159118 27.48927293387379 26.704882723063616 24.22620965690348 22-23 22.44288791275885 27.278547563726406 27.30992317215881 22.968641351355934 24-25 22.291521801807914 27.967962959822263 26.695978834184146 23.044536404185674 26-27 21.530451299967776 28.094737377677525 27.348930685345046 23.02588063700965 28-29 21.894662754608824 27.08011804012686 28.56706748299781 22.458151722266507 30-31 21.776792225633024 27.037718569272258 27.965842986279533 23.21964621881519 32-33 22.339433203873615 27.40277801333039 26.985143225412543 23.272645557383445 34-35 22.442039923341756 26.987263198955276 27.84500449434391 22.725692383359057 36-37 22.956769499516646 27.05340637348846 26.996167087834742 22.99365703916015 38-39 21.625850109390633 27.06061428353374 27.79370113460984 23.519834472465785 40-41 21.55037905126944 26.140121771280295 28.827824228753624 23.48167494869664 42-43 22.28346590234554 26.117650051727352 28.38220579007174 23.216678255855367 44-45 20.61165476654851 26.263928226175736 29.010989942845512 24.113427064430233 46-47 21.643657887149566 25.189101640011536 29.23061920187236 23.936621270966537 48-49 20.69560571884063 25.571120872411512 29.675389651137152 24.057883757610703 50-51 20.025270084629344 26.010379390465204 29.627054254362907 24.337296270542545 52-53 19.434221460916167 26.257568305547547 30.497939385716467 23.81027084781982 54-55 18.88514831334905 26.66799518342011 30.152807692959993 24.294048810270848 56-57 19.326526804945473 26.51917304072045 29.24885097433984 24.905449179994232 58-59 18.83172498007225 26.53994878143921 29.04787748248902 25.580448755999523 60-61 19.309991011312178 27.0703661618303 28.47675661007751 25.142886216780013 62-63 18.464969557179927 27.38751420382274 28.221087800824247 25.926428438173094 64-65 19.119193392466464 28.223631769075524 26.205416956396384 26.45175788206163 66-67 19.2705595034174 27.826348727167883 25.804741956820376 27.098349812594343 68-69 19.131065244305752 27.79370113460984 26.11722605701881 26.9580075640656 70-71 19.544460085138137 27.83610060546444 25.77803029018198 26.84140901921544 72-73 19.627139053304614 27.31331512982718 25.432898597425503 27.626647219442702 74-75 20.074453470820682 27.674558621508403 25.371419364686325 26.879568542984583 76 20.517103946542747 27.48842494445669 25.253124841001984 26.74134626799858 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.5 12 1.0 13 0.5 14 0.0 15 4.0 16 8.0 17 8.0 18 9.5 19 17.5 20 23.0 21 22.0 22 39.0 23 92.0 24 131.0 25 134.0 26 231.0 27 376.0 28 554.0 29 684.0 30 789.0 31 1098.0 32 1522.5 33 1743.0 34 1981.0 35 2608.5 36 3252.0 37 3506.0 38 3996.0 39 5062.0 40 6188.0 41 7097.5 42 7457.0 43 7831.0 44 8593.5 45 9098.0 46 9214.0 47 9071.5 48 8474.0 49 7681.0 50 7343.0 51 6795.5 52 5602.5 53 4615.0 54 4273.0 55 4028.0 56 3186.5 57 2306.5 58 2023.0 59 1719.5 60 1195.0 61 841.5 62 709.0 63 614.0 64 445.0 65 321.0 66 217.0 67 163.0 68 118.0 69 50.0 70 21.0 71 15.0 72 11.5 73 6.0 74 2.5 75 1.0 76 0.5 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 117926.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 40.25744958702916 #Duplication Level Percentage of deduplicated Percentage of total 1 61.16189914479505 24.62222071468548 2 15.121961494712895 12.175432050608007 3 8.107595736613725 9.791733799162186 4 4.423473901503981 7.123111103573428 5 2.563508446728736 5.160015603005275 6 1.779921641319459 4.299306344656819 7 1.2743817668618613 3.5912351813849366 8 0.8889076125879429 2.862812272102844 9 0.714075072671357 2.5872157115479197 >10 3.907401946328517 25.511761613215068 >50 0.050553987445759785 1.1948170886827334 >100 0.004212832287146649 0.5266014280141784 >500 0.0021064161435733245 0.5537370893611249 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGT 653 0.5537370893611249 No Hit ATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGTCTT 387 0.3281719044146329 RNA PCR Primer, Index 30 (95% over 22bp) ATAAAGAATATTGAGGCGCCATTGGCGTGAAGGTAGCGGATGATTCAGCC 234 0.19842952359954547 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0033919576683682988 0.0 2 0.0 0.0 0.0 0.0033919576683682988 0.0 3 0.0 0.0 0.0 0.0033919576683682988 0.0 4 0.0 0.0 0.0 0.004239947085460373 0.0 5 0.0 0.0 0.0 0.004239947085460373 0.0 6 0.0 0.0 0.0 0.005087936502552448 0.0 7 0.0 0.0 0.0 0.005935925919644523 0.0 8 0.0 0.0 0.0 0.005935925919644523 0.0 9 0.0 0.0 0.0 0.011871851839289045 0.0 10 0.0 0.0 0.0 0.027983650764038463 0.0 11 0.0 0.0 0.0 0.03137560843240676 0.0 12 0.0 0.0 0.0 0.03476756610077506 0.0 13 0.0 0.0 0.0 0.037311534352051284 0.0 14 0.0 0.0 0.0 0.04239947085460374 0.0 15 0.0 0.0 0.0 0.045791428522972036 0.0 16 0.0 0.0 0.0 0.046639417940064105 0.0 17 0.0 0.0 0.0 0.05087936502552448 0.0 18 0.0 0.0 0.0 0.05511931211098486 0.0 19 0.0 0.0 0.0 0.06614317453318183 0.0 20 0.0 0.0 0.0 0.07207910045282635 0.0 21 0.0 0.0 0.0 0.07886301578956295 0.0 22 0.0 0.0 0.0 0.08140698404083917 0.0 23 0.0 0.0 0.0 0.0839509522921154 0.0 24 0.0 0.0 0.0 0.08734290996048369 0.0 25 0.0 0.0 0.0 0.090734867628852 0.0 26 0.0 0.0 0.0 0.09497481471431236 0.0 27 0.0 0.0 0.0 0.12804640198090328 0.0 28 0.0 0.0 0.0 0.1602699998304021 0.0 29 0.0 0.0 0.0 0.1874056611773485 0.0 30 0.0 0.0 0.0 0.2315011108661364 0.0 31 0.0 0.0 0.0 0.2823804758916609 0.0 32 0.0 0.0 0.0 0.32562793616335667 0.0 33 0.0 0.0 0.0 0.3790512694401574 0.0 34 0.0 0.0 0.0 0.4562183063955362 0.0 35 0.0 0.0 0.0 0.5918966131302681 0.0 36 0.0 0.0 0.0 0.7945660838152739 0.0 37 0.0 0.0 0.0 1.1405457659888405 0.0 38 0.0 0.0 0.0 1.6196597866458626 0.0 39 0.0 0.0 0.0 2.2649797330529315 0.0 40 0.0 0.0 0.0 2.9238675101334737 0.0 41 0.0 0.0 0.0 3.767616980140088 0.0 42 0.0 0.0 0.0 4.798772111324051 0.0 43 0.0 0.0 0.0 5.8434950731814865 0.0 44 0.0 0.0 0.0 6.8432745959330425 0.0 45 0.0 0.0 0.0 7.873581737699913 0.0 46 0.0 0.0 0.0 8.820785916591761 0.0 47 0.0 0.0 0.0 9.87059681495175 0.0 48 0.0 0.0 0.0 10.69653850719943 0.0 49 0.0 0.0 0.0 11.441921204823364 0.0 50 0.0 0.0 0.0 12.16780014585418 0.0 51 0.0 0.0 0.0 13.01409358411207 0.0 52 0.0 0.0 0.0 14.091040143819004 0.0 53 0.0 0.0 0.0 15.034004375625392 0.0 54 0.0 0.0 0.0 16.032087919542764 0.0 55 0.0 0.0 0.0 16.948764479419296 0.0 56 0.0 0.0 0.0 17.723826806641455 0.0 57 0.0 0.0 0.0 18.424266065159507 0.0 58 0.0 0.0 0.0 19.13233722843139 0.0 59 0.0 0.0 0.0 19.769177280667538 0.0 60 0.0 0.0 0.0 20.401777385818225 0.0 61 0.0 0.0 0.0 21.038617438054374 0.0 62 0.0 0.0 0.0 21.680545426793074 0.0 63 0.0 0.0 0.0 22.40727235724098 0.0 64 0.0 0.0 0.0 23.14163119244272 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTGTAC 15 0.0022199422 70.0 10 CTGTACC 15 0.0022199422 70.0 2 GTACTCG 15 0.0022199422 70.0 13 GACGGTT 15 0.0022199422 70.0 11 TACTCGT 15 0.0022199422 70.0 14 CGTTTAA 15 0.0022199422 70.0 55 ACGGTTT 15 0.0022199422 70.0 12 AAATGCG 25 2.3822158E-4 56.000004 5 CTTCGAG 20 0.0069366503 52.5 31 ACGCCTT 20 0.0069366503 52.5 10 TACTTCG 20 0.0069366503 52.5 29 CCCAGAT 20 0.0069366503 52.5 29 ACTTCGA 20 0.0069366503 52.5 30 TTCCGGC 20 0.0069366503 52.5 41 CGCTCGT 20 0.0069366503 52.5 12 ACGGCAT 20 0.0069366503 52.5 57 TGCGAAT 20 0.0069366503 52.5 30 CGGTGTA 20 0.0069366503 52.5 9 GACGGCA 20 0.0069366503 52.5 56 TCGTAGG 20 0.0069366503 52.5 17 >>END_MODULE