FastQCFastQC Report
Wed 25 May 2016
SRR1780555_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780555_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences73
Sequences flagged as poor quality0
Sequence length50
%GC58

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTATGCAATGAGCGATTTTAGGTCTGTTTGTCGTAGGCAGATGGAGCTT11.36986301369863No Hit
GTCGCAGCTCTGCCATGGCGGTGGTCCCTCCCACATCTGTCTCTTATACA11.36986301369863No Hit
CAGCACGACGCCGGCTACGACGTCGCGGACTACCGCGCCGTGGACCCGCG11.36986301369863No Hit
CTTATACACATCTGACGCTGCCGACGAACTCCCAGGAGGAGAACTCGGTG11.36986301369863No Hit
TTTTTGACAGGGTGCTGATTGGTGCGTTTACAATACCTGAGCTAGATACA11.36986301369863No Hit
CTATTAGCTCTTAGTAAGATTACACATGCAAGCATCCCCATTCCAGTGAG11.36986301369863No Hit
CGCCAGGACCGCGTGATGACGCGCATGACCTGGGCGCTGTCTCTTATACA11.36986301369863No Hit
GAATAATAGGTATAGTGTTCCAATGTCTTTGTGGTTTGTAGAGAATAGTC11.36986301369863No Hit
ACCCTAGCAATATCAACCATTAACCTTCCCTCTACACTTATCATCTTCAC11.36986301369863No Hit
ACCAAAGAGCCCCTAAAACCCGCCACATCTGGCCTCACCCTCTACATCAC11.36986301369863No Hit
AGATGGAGGCCTACCGTGGGAAGGTCCGTGATGTGGCTGAGGCATCGTCC11.36986301369863No Hit
GGATTATGATGCGACTGTGAGTGCGCTCGTAGTTTGAGTTTGCTAGGCAG11.36986301369863No Hit
GTCCATGCCGGTGCGGTTCGCGCTGGAGACGGCCATGGGGCCGACGGCGT11.36986301369863No Hit
GGCCGGTAGAAGTCGTGGCCGCGCAGCAGCTCCACCACGTCCGCGATCGC11.36986301369863No Hit
TCCGAACTCTGGGAAGTCCCCAAGCAAGACTGACCTAGGTGCACACACCT11.36986301369863No Hit
GTCGTGATGACCACCGAGGTGCTGCGCAACATGCTGTACGCCTGTCTCTT11.36986301369863No Hit
CTCGAAGGCGCCCGGGGTGATGTGGTAGGCGAGGTTGAAGCCGTCCACGT11.36986301369863No Hit
GACCCAAACATTAATCAGTTCTTCAAATATCTACTCATCTTCCTAATTAC11.36986301369863No Hit
GTGGTAGACGACGTGCCCGGCCAGGACCCCCGCGTACCGGGACCGCTCCC11.36986301369863No Hit
CTCTTGTGCGGGATATTGATTTCACGGAGGATGGTGGTCAAGGCACCCCT11.36986301369863No Hit
CCCGGGGGAGGGCGGTTACCGAGGCCCGCCACCGCCGGAAGGGTGGGTTC11.36986301369863No Hit
GCAGAGTCCAGGCGCTGCGCTCCCTCGCCGACGCCCGGGCGGCGGCCGGG11.36986301369863No Hit
TCCCCACCTTGGCTATCATCACCCGATGAGGCAACCAGCCAGAACGCCTG11.36986301369863No Hit
GCCGCGAGATGGCCGTCTCCATCCCCCTGCGCCGCGACGACGGCTCCACC11.36986301369863No Hit
CCCTTGCGGTGCGTGGTGCCGATCGGGTGGATCGGCGGCAGGTAGGCCAC11.36986301369863No Hit
GAACTAGTCAGTTGCCAAAGCCTCCGATTATGATGGGTCTGTCTCTTATA11.36986301369863No Hit
CTAGAAAAAGAGCCTGAATTGAGGCATAGGCCTTATTTTTATTACTTTGT11.36986301369863No Hit
CCGTTAGTCCGAGTAGACTTCTTGCAGTGCCCCCCATACCCGGCCTAAAA11.36986301369863No Hit
GTGCTGTGGGTGAAAGAGTATGGTGGGGGGGGGGTTGTGGTAAACTTTAA11.36986301369863No Hit
ATCCAGATAGGACCATACCCCTCCCTAAACCATGGCTGTCTGGAGGGTAG11.36986301369863No Hit
TAGCCTAGCCGTTTACTCAATCCTCTGATCAGGGTGAGCATCAAACTCAA11.36986301369863No Hit
ACCCCCGGGGCTGCCTGGCCTCATCCGCCCTCCACCCTAAAAAATCCACC11.36986301369863No Hit
CTTCTAAACGCTAATCCAAGCCTCACCCCACTACTAGGCCTCCTCCTAGC11.36986301369863No Hit
CGTCGGTGGAGCCGCCCGGGACGACGACGCCGGACACGGTCTGTCTCTTA11.36986301369863No Hit
TCGGTGAGCCGCTCCTCCGGGTCGGACAGCTGTCTCTTATACACATCTGA11.36986301369863No Hit
CGCGGCGGCCTTGGCGACCACGAGCAGGCCCGGGACCGTGGTGCGCGCGC11.36986301369863No Hit
GTTGTGGTAGTCAAAATGTAATAATTATTAGTAGTAAGGCTAGGAGGGTG11.36986301369863No Hit
CAGAAGGAGGGGGGTGGGAAGAGGGAGGAGCGGAGCGCAGCGGCCGGGGG11.36986301369863No Hit
ATACTGTACATAAAATATGAAAGTACATCAAATATATATTTTATTCTGTA11.36986301369863No Hit
GCGGTGTGTGTGTGCTGGGTAGGATGGGCGGGGGTTGTATTGATGAGATT11.36986301369863No Hit
GTCGTAGGCGCCGAAGAGGTAGCCGTGGTCCGTGATGGCCAGCGAGTCCA11.36986301369863No Hit
GGTGTATATCGCCAAGAATTTTTCGTTCGTTAAGCATTAGGGAAGCCCTT11.36986301369863No Hit
CTTTAGTGTTGTGTATGGTTCTTATTTGTTTTGCGGTTACTTTGACCTGT11.36986301369863No Hit
TTACCACTGCGTAAAAAAAGAACCATTTGGATACATAGGTATGGTCTGCG11.36986301369863No Hit
GTGAATAAGAGTCCTATAGCCACAGCTGATCTACAGATCGTAGAGTTCCC11.36986301369863No Hit
GGTGGCCACCGTGGGCCGGCTGACGCGGAACTCGGCGGCCACGTGTGCCT11.36986301369863No Hit
GTGCGGGGGCCCCTGGGCGTCGTGGGCGCCGTGTACGAGGCCCGTCCGAA11.36986301369863No Hit
TTTTGGTTCTCAGGGTTTGTTATAATTTTTTATTTTTATGGGCTTTGGTG11.36986301369863No Hit
GATGAAAAATTATAACCAAGCATAATATAGCAAGGACTAACCCCTATACC11.36986301369863No Hit
CTACTATGCCTAGAAGGAATAATACTATCGCTGTTCATTATAGCTACTCT11.36986301369863No Hit
CGCACGACGTCGGCCGCCCGCGCCGACGCGTCCGGCGCTGTCTCTTATAC11.36986301369863No Hit
GCTCATGGGTGAACTNGNNNNTCNCCTTGGCGACGNGCANCCGGAGGGCT11.36986301369863No Hit
AGCTCGTAGTGTTCTGGCGAGCAGTTTTGTTGATTTAACTGTTGAGGTTT11.36986301369863No Hit
GGCTCGACGAGGCCGGGGTCCGCGCCGCCCTGGACGACCTGTCTCTTATA11.36986301369863No Hit
GGCTATGTCCTCCCGTGAGGCCAAATATCATTCTGAGGGGCCCTGTCTCT11.36986301369863No Hit
TCCACGCACCCCGCCCATCCGCTGCCGCCCGTGGCGCCCCGTCGTCTGCT11.36986301369863No Hit
GGCGGGCGCTCGCGGTGCGACTGCACGCGGCGTGGCGGGGGGGCCCCCTT11.36986301369863No Hit
GTACACATCTGACGCTGCCGACGAACCTGGCGGCGGAGCTCCCGGGTGTC11.36986301369863No Hit
AGCCCGGACCCACCGGGGCCCGGGCCGTGGCGCGGTGTCGTGTCAGGCGG11.36986301369863No Hit
CGCCCCCGCTGGGGCTGCTCGCCGTTCCAGGCGATGCAGGCCCTGGCCGC11.36986301369863No Hit
CGGGGTGACGGTGCAGTCCACGATCGTCGAGGGATCCCGGCTCTGTCTCT11.36986301369863No Hit
GAGCGGCACCCTCCGGCCAGCCACTCTCCCGCCCCCCCGCTGCCCCTTCC11.36986301369863No Hit
GAACCACTATCACGAAAAAAACTCTACCTCTCTATACTAATCCTGTCTCT11.36986301369863No Hit
CTGCAGGCCCAGCTCGAGGCGGACCGGGCCTGGGTGCCCACCGGCCCTGT11.36986301369863No Hit
TCGCCGAACAGCGGCAGGTCGGTGAACTCGACGACGGCGGACTGCCGCAC11.36986301369863No Hit
CCCGCCCCCTCTACCTTTATCCTCCTTGTTACCTGCCACACCCTTCCCCT11.36986301369863No Hit
GAGGTGCGTTTGGAGTTGGGTTATTTCGGGGGGGGGCGCATTTCTACGTT11.36986301369863No Hit
CCCCAAATAAGCTTCTTCCTGACTGGTTTCTAGAATCTCATCTTGCACCC11.36986301369863No Hit
ACCCCGAGGCGTACCAGCAGGCCGCCTACGCCACGGCGCGTCTCGAGCCC11.36986301369863No Hit
GGTCGCCTAGGAGGTCTGGTGAGAATAGTGTTAATGTCATTAAGGAGAGA11.36986301369863No Hit
ATATAGGTAGGAGTGACGGGGGAAGGGCGATGAGTGTGGGGCGGAAAGAG11.36986301369863No Hit
CGGTCCGGGATGCGGTCCGCGGGCAGGGAGCGCACGGCGGTGACGGTCAT11.36986301369863No Hit
CTGCTGGCTTGATCGACTCGGGGTTCACGGCGTTCTTCTCCGGCACGAGC11.36986301369863No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTTTG50.044.032
GAGAATA50.044.041
CAATGTC50.044.021
GTAGAGA50.044.038
GTTTGTA50.044.034
CCAATGT50.044.020
GTGTTCC50.044.015
TAGTGTT50.044.013
TGTCTTT50.044.024
TTGTAGA50.044.036
ATAGGTA50.044.06
GTTCCAA50.044.017
TGTTCCA50.044.016
GGTTTGT50.044.033
GTATAGT50.044.010
ATAATAG50.044.03
AGAATAG50.044.042
AATAGTC50.044.044
AGTGTTC50.044.014
ATAGTGT50.044.012
Produced by FastQC (version 0.11.5)