FastQCFastQC Report
Wed 25 May 2016
SRR1780555_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780555_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences73
Sequences flagged as poor quality0
Sequence length50
%GC59

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGCAGACGACGGGGCGCCACGGGCGGCAGCGGATGGGCGGGGTGCGTGG11.36986301369863No Hit
ATACACATCTCCGAGCCCACGAGACCATCCTGTATCTCGTATGCCGTCTT11.36986301369863TruSeq Adapter, Index 2 (95% over 21bp)
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCC11.36986301369863No Hit
GCACCTGGATCAGGACCGCATTGGTGGACTCCGCGGCCGAGCCGCCGCGC11.36986301369863No Hit
AGCTCGGCCGGCTGCGCACCCGGATCCGCCAGGCGGCCAACGCGTGCGAC11.36986301369863No Hit
GGCAAGGAACCCACCCTTCCGGCGGTGGCGGGCCTCGGTAACCGCCCTCC11.36986301369863No Hit
ATGTGGGAGGGACCACCGCCATGGCAGAGCTGCGACCTGTCTCTTATACA11.36986301369863No Hit
CTTCGACGAGGCCTCCGGCACGTGGTCCTCCGGCACCTTCGCGACGGCGG11.36986301369863No Hit
GAGTAGTAGAATGTTTAGTGAGCCTAGGGTGTTGTGAGTGTAAATCAGTG11.36986301369863No Hit
CTTCCACCGTTCCGTGGTCGAGTCCTTCGGCGGCTCCCTGGTCTCGTTCT11.36986301369863No Hit
AACCGCGGGTCCACGGCGCGGTAGTCCGCGACGTCGTAGCCGGCGTCGTG11.36986301369863No Hit
CTGTCCGACCCGGAGGAGCGGCTCACCGACTGTCTCTTATACACATCTAC11.36986301369863No Hit
CTTATACACATCTCCGAGCCCAAGAGACGATCCAAAATCTCGTATGCCGT11.36986301369863No Hit
TCCTTGTACTATCCCTATGAGGCATAATTATAACAAGCTCCATCTGCCTA11.36986301369863No Hit
GTCGCCCTGCGCGTGCCCGACGACGCGGTGGCCCGCGAGCTGCTCTACGC11.36986301369863No Hit
CGCCGGACGCGTCGGCGCGGGCGGCCGACGTCGTGCGCTGTCTCTTATAC11.36986301369863No Hit
GAGCCGAACACGATCAGGGTGCGGCCGCGCGTCCCGGCCGCCGCCCGGGC11.36986301369863No Hit
AGTCAGAGAGGAGGGGAAGGGTGTGGCAGGGAACAAGGAGGGTAAAGGGA11.36986301369863No Hit
TCTGAACACACAATAGCTAAGACCCAAACTGGGATTAGATACCCCACTAT11.36986301369863No Hit
CCGCTGTTCTTCGGCGGTCAGGTGCTGCAGTCCGCCGTCGTCGAGTTCAC11.36986301369863No Hit
ATAGTTGAGGGTTGATTGCTGTACTTGCTTGTAAGCATGGGGAGGGGGTT11.36986301369863No Hit
GGCGAACGCGGGGGCGGGCTCGAGACGCGCCGTGGCGTAGGCGGCCTGCT11.36986301369863No Hit
ATCAAAGACGCCCTCGGCTTACTTCTCTTCATTCTCTCCTTAATGACATT11.36986301369863No Hit
AGAGAGAGGAGAGGAAGAGAGAGAGAGAAAGAGAAGGAGAAGCAGCTTAG11.36986301369863No Hit
CCAGGGAACTCTACGATCTGTAGATCAGCTGTGGCTATATGACTCTTATT11.36986301369863No Hit
ACCGACGACGCCGCAGAGGGGGAGGCCTCGGCATGAGCGCGCGCACCACG11.36986301369863No Hit
GTTTGAGTTTGATGCTCACCCTGATCAGAGGATTGAGTAAACGGCTAGGC11.36986301369863No Hit
ATCCTGATGATCAGCCTGTACGTCCTCGTGGCCCTCGTGCTCATCGAGAG11.36986301369863No Hit
CTTATACACATCTCCGAGCCCACGAACTTGACCCTATCTCGTATGCCGTC11.36986301369863No Hit
GAGGAAAAGGTTGGGGAACAGCTAAATAGGTTGTTGTTGATTTGGTTAAA11.36986301369863No Hit
CCTCACGCCGGCAGCGTGGTCGCTGACCCCCGTGACGGCTGTCTCTTATA11.36986301369863No Hit
CCTCACCCCCACTGATGTTCGCCGACCGTTGACTATTCTCTACAAACCAC11.36986301369863No Hit
GCGAAGTGGTCCTCCCGCCACGCCGCGTGCAGTCGCACCGCGAGCGCCCG11.36986301369863No Hit
GGCCCCTCAGAATGATATTTGGCCTCACGGGAGGACATAGCCCTGTCTCT11.36986301369863No Hit
CCAGCACGGAGCGCTTGAGGCGCACGGTGGTGGCCGGCGGCGTCTCGAGC11.36986301369863No Hit
AGCCCACGAGACCGAGCCCACGAGACATGATGATATCTCGTATGCCGTCT11.36986301369863No Hit
AGCCGGGATCCCTCGACGATCGTGGACTGCACCGTCACCCCGCTGTCTCT11.36986301369863No Hit
GCCGAGACGGACCCGGGCACCCACGGCCTGCCCGCGTCCGAGCCGATGGC11.36986301369863No Hit
AGCCGCGGAGCACCTCGGTGGAGCCGTCGTCGCGGCGCAGGGGGATGGAG11.36986301369863No Hit
CTTATACACATCTCCGACCCACGAGACAATTCGTTATCTCGTATGCCGTC11.36986301369863No Hit
TTCTAGGATAGTCAGTAGGATTAGAATTGTGAAGATGATAAGTGTAGAGG11.36986301369863No Hit
TCTCCGAGCCCACGAGACGATCCAAAATCTCGTATGCCGTCTTCTGCTTG11.36986301369863Illumina PCR Primer Index 6 (96% over 29bp)
GTGCTGGGCGGCATGATGCTCTCCAAGGACGCGATCGCGGACGTGGTGGA11.36986301369863No Hit
ACCCATCATAATCGGAGGCTTTGGCAACTGACTAGTTCCTGTCTCTTATA11.36986301369863No Hit
CTCGCGATCCGGGCACGCCTTTCCCGAATGTTGGAAGCTGAGCTGCGAGC11.36986301369863No Hit
GCCTCGACCTGTCCGAGGCCATCACCGCCTCCGAGCCCACGAGACTAACA11.36986301369863No Hit
CCTTTTGATCGTGGTGATTTAGAGGGTGAACTCACTGGAATGGGGATGCT11.36986301369863No Hit
GATTAGTATAGAGAGGTAGAGTTTTTTTCGTGATAGTGGTTCCTGTCTCT11.36986301369863No Hit
GTCCACCGCCACCTGCACGACGGGATCCCGCAGGCACACGTGGCCGCCGA11.36986301369863No Hit
ACCGTGTCCGGCGTCGTCGTCCCGGGCGGCTCCACCGACGCTGTCTCTTA11.36986301369863No Hit
GGGGTGGAGACCTAATTGGGCTGATTTGCCTGCTGCTGCTAGGAGGAGGC11.36986301369863No Hit
CTAAAAATATTAAACACAAACTACCACCTACCTCCCTCACCAAAGCCCAT11.36986301369863No Hit
GTAATAAACTTCGCCTTAATTTTAATAATCAACACCCTCCTAGCCTTACT11.36986301369863No Hit
GATGAGGAGTGGACCGTGCGCAAGCTCGCCGAATGGGTGGGCATCGGCGG11.36986301369863No Hit
GTCCCGGAGGAGGCAGTTTGCCCGGAGCCCTGGGGGGCGGCGTCGCGGGG11.36986301369863No Hit
ATCTCCGAGCCCATGAGACGGTAAACCATCTCGTATGCCGTCTTCTGCTT11.36986301369863RNA PCR Primer, Index 36 (96% over 27bp)
TGAGATGGCCGCGCCCACCCCGAGCCCACGAGACATGATGATATCTCGTA11.36986301369863No Hit
GCGTACAGCATGTTGCGCAGCACCTCGGTGGTCATCACGACCTGTCTCTT11.36986301369863No Hit
CGCCCAGGTCATGCGCGTCATCACGCGGTCCTGGCGCTGTCTCTTATACA11.36986301369863No Hit
CGGCTGGACCGTGCTCCTGGACGGCACCCACCGGCTCCGCCGGCTCTCCG11.36986301369863No Hit
CTCCCATACTACTAATCTCATCAATACAACCCCCGCCCATCCTACCCAGC11.36986301369863No Hit
CCCCTGGGGCGAGGACGGCACCCTCCAGGGCATGTTCGAGTCGCTGGGTC11.36986301369863No Hit
GTATCTAGCTGCTCTGGTGGGGCCTTGGAGAACCTGTGTGTCAAAACTCT11.36986301369863No Hit
GCCTAAGCGATACCTGTCTCTCAAAGCGAGGCTCGGCCGCCGCCACCACC11.36986301369863No Hit
CCCACCGGCTGCTCGTCGCCAAGGCGATCCGCGAGTTCACCCATGAGCCT11.36986301369863No Hit
ACGCCGGGGGAGCGGTCCCGGTACGCGGGGGTCCTGGCCGGGCACGTCGT11.36986301369863No Hit
GGCCGGTGGGCACCCAGGCCCGGTCCGCCTCGAGCTGGGCCTGCAGCTGT11.36986301369863No Hit
GCTGAGAAGTTCAGGTGTGTGCACCTAGGTCAGTCTTGCTTGGGGACTTC11.36986301369863No Hit
CCAGGGAACTCTACGATCTGTAGATCAGCTGTGGCTATAGGACTCTTATT11.36986301369863No Hit
CGACCTGTTCGCCGAGGCCCAGCGGCTGGGCATGGACTCGCTGGCCATCA11.36986301369863No Hit
CCTCTCCTTCATAAATTATTCAGCTTCCTACACTATTAAAGTTTACCACA11.36986301369863No Hit
GTCGTCCAGGGCGGCGCGGACCCCGGCCTCGTCGAGCCCTGTCTCTTATA11.36986301369863No Hit
CCCTATGAGGCATAATTATAACAAGCTCCATCTGCCTACGACAAACAGAC11.36986301369863No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACAAA50.044.040
ACAAACC50.044.042
GTTGACT50.044.028
AAACCAC50.044.044
ACTGATG50.044.011
TTCGCCG50.044.018
TGACTAT50.044.030
GATGTTC50.044.014
ACCGTTG50.044.025
TGTTCGC50.044.016
CCCACTG50.044.08
CCGTTGA50.044.026
CTGATGT50.044.012
CACTGAT50.044.010
TCTACAA50.044.039
CTCTACA50.044.038
CCACTGA50.044.09
CACCCCC50.044.04
TTCTCTA50.044.036
TCGCCGA50.044.019