FastQCFastQC Report
Wed 25 May 2016
SRR1780554_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780554_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6541
Sequences flagged as poor quality0
Sequence length76
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGTCAGATGTGTATAAGCTGTCTCTTATACACATCTGACGCTGCCGACG80.12230545788105794No Hit
CTTATACACATCTGACGCTGCCGACGATGCACGAAGTGTAGATCTCGGTG70.10701727564592571No Hit
ATTCCTTATATTCCCTAAATTCCCAGTCAGTCTGTGAACTGTCTCTTATA70.10701727564592571No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCCCT200.006646631352.540
CACATCT1000.00872069517.57
Produced by FastQC (version 0.11.5)