FastQCFastQC Report
Wed 25 May 2016
SRR1780554_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780554_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6541
Sequences flagged as poor quality0
Sequence length76
%GC41

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGT237636.32472099067421No Hit
ATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGTCTT66810.212505733068339Illumina PCR Primer Index 10 (95% over 23bp)
TCTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCG3405.197981959944962No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCC2864.372420119247821No Hit
CTTTACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGTC1051.6052591346888856Illumina PCR Primer Index 10 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACAGTAGATTATATCGTATGCCGT981.4982418590429598No Hit
TATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGTCT811.2383427610457116Illumina PCR Primer Index 10 (95% over 22bp)
CTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGTC741.131325485399786Illumina PCR Primer Index 10 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACAGTAGATTAACTCGTATGCCGT600.9172909341079345No Hit
CTTATACACATCTCCGAGCCCACGAGACAGTAGATTACCTCGTATGCCGT550.8408500229322734No Hit
TCTTATACACATCTCCGAGCCCACGAGACAGTAGATTAACTCGTATGCCG270.41278092034857056No Hit
CTTATACACATCTCCGAGCCCACGAGACAGTAGCTTATCTCGTATGCCGT260.3974927381134383TruSeq Adapter, Index 10 (95% over 23bp)
ATACACATCTCCGAGCCCACGAGACAGTAGATTAACTCGTATGCCGTCTT250.38220455587830604No Hit
ACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGTCTTCT230.3516281914080416Illumina PCR Primer Index 10 (96% over 25bp)
ATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGTCTTCTGCTT200.3057636447026449Illumina PCR Primer Index 10 (96% over 29bp)
TCTTAATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCC190.2904754624675126No Hit
CTTATACACATCTCCGAGCCCACGAGCCAGTAGATTATCTCGTATGCCGT190.2904754624675126No Hit
CTTATACACATCTCCGAGCCCACGCGACAGTAGATTATCTCGTATGCCGT170.25989909799724814No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAGTAGATTATATCGTATGCC170.25989909799724814No Hit
CTTATACACATCTCCGAGCCCACGAGACAGTAGATTAGCTCGTATGCCGT160.24461091576211588No Hit
TCTTATACACATCTCCGAGCCCACGAGACAGTAGATTATATCGTATGCCG160.24461091576211588No Hit
ATACACATCTCCGAGCCCACGAGACAGTAGATTACCTCGTATGCCGTCTT150.22932273352698362No Hit
CTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTAGTATGCCGT150.22932273352698362No Hit
CTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCAGT150.22932273352698362No Hit
CTTATACACATCTCCGAGCCCACGAGACCGTAGATTATCTCGTATGCCGT140.21403455129185142No Hit
TTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGTC140.21403455129185142Illumina PCR Primer Index 10 (95% over 21bp)
CTTATACACATCTACGAGCCCACGAGACAGTAGATTATCTCGTATGCCGT130.19874636905671916No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAGTAGATTAACTCGTATGCC130.19874636905671916No Hit
ATACACATCTCCGAGCCCACGAGACAGTAGATTATATCGTATGCCGTCTT130.19874636905671916No Hit
CTTATACACATCTCCGAGCCCAAGAGACAGTAGATTATCTCGTATGCCGT120.1834581868215869No Hit
CTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTCTGCCGT120.1834581868215869No Hit
CTTATACACATCTCCGAGCACACGAGACAGTAGATTATCTCGTATGCCGT120.1834581868215869No Hit
CTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGC110.16817000458645467No Hit
CTTATACACATCTCCGAGCCCACGAGAAAGTAGATTATCTCGTATGCCGT110.16817000458645467No Hit
TCTTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCC110.16817000458645467No Hit
CTTATACACATCTGACGCCTGTCTCTTATACACATCTCCGAGCCCACGAG100.15288182235132244No Hit
CTTATACACATCTCCGAGCCCACGAGACAGTAGATTATTTCGTATGCCGT100.15288182235132244No Hit
ATTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGT100.15288182235132244No Hit
CTTATACACATCTCCGAGCCCACGTGACAGTAGATTATCTCGTATGCCGT100.15288182235132244No Hit
CTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGACGT100.15288182235132244No Hit
CTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCCCGTATGCCGT90.13759364011619019No Hit
CTTATCACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGTC90.13759364011619019Illumina PCR Primer Index 10 (95% over 21bp)
CTTATACACATATCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGT90.13759364011619019No Hit
TCTTATACACATCTCCGAGCCCACGAGACAGTAGATTACCTCGTATGCCG80.12230545788105794No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAGTAGATTACCTCGTATGCC80.12230545788105794No Hit
CTTATTACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCG80.12230545788105794No Hit
GACAACATTCTTTTACTTCAAATGCTTGACATAGTGCTTTCCTTTAACTG80.12230545788105794No Hit
CTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCACGTATGCCGT80.12230545788105794No Hit
TTCACAGACTGACTGGGAATTTAGGGAATATAAGGAATCTGTCTCTTATA70.10701727564592571No Hit
CTTATACACATCTCCGAGCCCACGAGACAGTAGATTACATCGTATGCCGT70.10701727564592571No Hit
CTTATACACATCTCCGAGCCCACGAGACAGTAGATCATCTCGTATGCCGT70.10701727564592571No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAGTAGATTCTCTCGTATGCC70.10701727564592571No Hit
ATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGTCCT70.10701727564592571Illumina PCR Primer Index 10 (95% over 21bp)

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGCAAA150.002126429270.0000158
CTATACA150.002126429270.000011
AGCCAGT150.002126429270.0000125
TTACCTC150.002126429270.0000135
CCAGTAG150.002126429270.0000127
TGCAAAA150.002126429270.0000159
GAGCCAG150.002126429270.0000124
CACGAGC150.002126429270.0000121
GCCAGTA150.002126429270.0000126
ATTACCT150.002126429270.0000134
GCAAAAA150.002126429270.0000160
TGCTTGC150.002126429270.0000155
GCTTGCA150.002126429270.0000156
ACCTCGT150.002126429270.0000137
CTTGCAA150.002126429270.0000157
GATTACC150.002126429270.0000133
TACCTCG150.002126429270.0000136
AGATTAC150.002126429270.0000132
CCTCGTA200.006646631352.538
ACGAGCC200.006646631352.522
Produced by FastQC (version 0.11.5)