##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780554_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 6541 Sequences flagged as poor quality 0 Sequence length 76 %GC 41 >>END_MODULE >>Per base sequence quality warn #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.731386638128726 34.0 34.0 34.0 31.0 34.0 2 33.279009325791165 34.0 34.0 34.0 32.0 34.0 3 33.45772817611986 34.0 34.0 34.0 34.0 34.0 4 33.48769301330072 34.0 34.0 34.0 34.0 34.0 5 33.483106558630176 34.0 34.0 34.0 34.0 34.0 6 37.3699740100902 38.0 37.0 38.0 37.0 38.0 7 36.805228558324416 38.0 37.0 38.0 34.0 38.0 8 36.92004280691026 38.0 37.0 38.0 35.0 38.0 9 36.678336645772816 38.0 37.0 38.0 34.0 38.0 10-11 36.347271059471026 38.0 37.0 38.0 34.0 38.0 12-13 36.118942057789326 38.0 37.0 38.0 33.0 38.0 14-15 36.096774193548384 38.0 36.5 38.0 32.5 38.0 16-17 36.000917290934105 38.0 36.0 38.0 32.0 38.0 18-19 35.889925087907045 38.0 36.5 38.0 31.5 38.0 20-21 35.698975691790245 38.0 36.0 38.0 31.0 38.0 22-23 36.08408500229323 38.0 36.5 38.0 32.5 38.0 24-25 34.66281914080416 37.0 34.0 38.0 28.0 38.0 26-27 34.85124598685216 37.0 34.0 38.0 28.5 38.0 28-29 34.938465066503596 37.0 35.0 38.0 29.0 38.0 30-31 34.61924782143403 37.0 34.5 38.0 26.5 38.0 32-33 34.957422412475154 37.0 35.5 38.0 27.0 38.0 34-35 33.84375477755695 37.0 34.0 38.0 25.5 38.0 36-37 34.0087907047852 37.0 34.0 38.0 25.5 38.0 38-39 30.890536615196453 34.5 27.0 37.0 11.0 38.0 40-41 32.78734138510931 36.5 32.5 37.5 18.5 38.0 42-43 34.57682311573154 37.0 34.5 38.0 28.0 38.0 44-45 35.161443204403 37.0 35.0 38.0 29.0 38.0 46-47 35.112138816694696 37.0 36.0 38.0 29.0 38.0 48-49 34.80820975386027 37.0 35.0 38.0 28.0 38.0 50-51 34.49205014523773 37.0 34.0 38.0 26.0 38.0 52-53 34.017734291392756 37.0 34.0 38.0 23.5 38.0 54-55 34.63736431738266 37.0 34.5 38.0 27.0 38.0 56-57 34.68888549151506 37.0 34.5 38.0 27.0 38.0 58-59 34.13239565815624 37.0 34.0 38.0 24.0 38.0 60-61 33.830530499923555 37.0 34.0 38.0 23.5 38.0 62-63 34.8608775416603 37.0 35.0 38.0 27.0 38.0 64-65 34.8096621311726 37.0 35.0 38.0 26.0 38.0 66-67 32.8900779697294 37.0 31.5 38.0 21.5 38.0 68-69 29.89175966977526 34.0 24.0 37.5 11.0 38.0 70-71 26.432731998165416 29.0 17.0 35.5 10.5 37.5 72-73 21.508026295673446 22.0 11.0 29.5 10.5 36.5 74-75 20.511007491209295 21.0 11.0 26.0 11.0 35.5 76 20.993884727105947 21.0 11.0 28.0 11.0 36.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.7969368402947481 1101 2 0.6314821981424146 1101 3 0.18325412344069747 1101 4 0.19930623449749163 1101 5 0.4153961399887649 1101 6 -0.15245109354914632 1101 7 0.9798239956185668 1101 8 0.45330769195952314 1101 9 0.2153910479242569 1101 10-11 0.6770993450473313 1101 12-13 1.1200904011188868 1101 14-15 1.2851101243821574 1101 16-17 1.2293351574240106 1101 18-19 -0.5223885064182738 1101 20-21 -0.4620615732442559 1101 22-23 1.1412493436894664 1101 24-25 0.6623827127804134 1101 26-27 0.1965259106874626 1101 28-29 0.6864850383828411 1101 30-31 2.240330848405847 1101 32-33 2.231390484402432 1101 34-35 1.8293367416218587 1101 36-37 2.8401714102076667 1101 38-39 -1.5530244600148464 1101 40-41 1.8662780855647938 1101 42-43 1.2960366198649353 1101 44-45 1.4980352551916454 1101 46-47 1.4953774238227027 1101 48-49 -2.001373160067338 1101 50-51 -1.9454192919088271 1101 52-53 -2.016149822117221 1101 54-55 0.6477970031502807 1101 56-57 -1.4998898982492292 1101 58-59 -2.4394304016620474 1101 60-61 -5.990898330708092 1101 62-63 -0.7129570976590145 1101 64-65 -2.86802805840712 1101 66-67 -0.14796979387323006 1101 68-69 0.04701344757663506 1101 70-71 -1.3538434337262153 1101 72-73 -4.301901772943715 1101 74-75 -1.1743845957127128 1101 76 -1.310428887621537 1102 1 -1.6867232904242044 1102 2 0.5334429824561369 1102 3 0.6538423587348134 1102 4 0.49342388155631767 1102 5 0.4186641138449758 1102 6 -0.005392270019733303 1102 7 -0.12475116778012563 1102 8 0.8879482148353404 1102 9 1.1173518322379792 1102 10-11 1.1672954234787127 1102 12-13 1.0825087017724826 1102 14-15 0.26550228124490616 1102 16-17 0.8698580332410089 1102 18-19 0.6998337158039476 1102 20-21 1.9268273156446298 1102 22-23 0.9941905201600534 1102 24-25 -5.409512712056191 1102 26-27 -1.5551080762406428 1102 28-29 2.068837979559312 1102 30-31 3.5099386915430983 1102 32-33 2.231390484402432 1102 34-35 0.8963302056741469 1102 36-37 -1.2186521192041013 1102 38-39 1.9845572393315614 1102 40-41 2.3613761247804703 1102 42-43 1.838520279995656 1102 44-45 2.4424796996360882 1102 46-47 2.3908022604240102 1102 48-49 -4.507909107779756 1102 50-51 -2.3359421677258254 1102 52-53 0.7534580210200374 1102 54-55 1.3847251077254512 1102 56-57 0.6912865723390027 1102 58-59 0.8759290754621318 1102 60-61 -4.051355847047958 1102 62-63 -1.949885202234178 1102 64-65 0.7398150788477764 1102 66-67 2.6036641930548754 1102 68-69 1.0077977613021254 1102 70-71 -0.20678461019680228 1102 72-73 -3.844385433074436 1102 74-75 -1.7234042035558517 1102 76 0.7745384326399041 1103 1 0.6910544873535756 1103 2 0.6167763157894726 1103 3 0.303842358734812 1103 4 0.4434238815563205 1103 5 0.32421966940053437 1103 6 0.3946077299802653 1103 7 0.897471054442093 1103 8 1.026837103724226 1103 9 0.10068516557131346 1103 10-11 0.47562875681204275 1103 12-13 -0.8063801871164031 1103 14-15 -1.1928310520884295 1103 16-17 -0.4162530778701026 1103 18-19 0.22761149358172617 1103 20-21 -0.5620615732442502 1103 22-23 0.5441905201600505 1103 24-25 -4.531734934278415 1103 26-27 -0.25788585401841857 1103 28-29 -0.6311620204406907 1103 30-31 0.3932720248764312 1103 32-33 2.1563904844024293 1103 34-35 0.48521909456303547 1103 36-37 0.08134788079589583 1103 38-39 0.1234461282204471 1103 40-41 -1.3719572085528604 1103 42-43 -1.6392574977821255 1103 44-45 0.39803525519164396 1103 46-47 1.1380244826462302 1103 48-49 -0.46902021889086853 1103 50-51 -0.19983105661471257 1103 52-53 0.8867913543533632 1103 54-55 1.3125028855032213 1103 56-57 1.6162865723389999 1103 58-59 -0.3879598134267539 1103 60-61 -3.854133624825735 1103 62-63 -1.6526629800119537 1103 64-65 0.5731484121811121 1103 66-67 -1.9435580291673453 1103 68-69 -0.5338689053645389 1103 70-71 0.0682153898031963 1103 72-73 -0.09160765529665582 1103 74-75 -0.3567375368891845 1103 76 0.7189828770843469 1104 1 -1.4878928810674772 1104 2 0.2878289473684177 1104 3 0.6012107797874435 1104 4 -0.19078664475946994 1104 5 0.15843019571632055 1104 6 -0.29486595423026074 1104 7 0.6237868439157808 1104 8 0.6452581563558084 1104 9 0.5033167445186848 1104 10-11 0.29010244102256877 1104 12-13 0.6015145497257066 1104 14-15 0.6953268426484129 1104 16-17 0.6797995537088397 1104 18-19 1.3723483356869863 1104 20-21 0.2221489530715388 1104 22-23 0.5731378885811083 1104 24-25 0.7366861183531626 1104 26-27 1.8250088828236812 1104 28-29 2.3319958742961546 1104 30-31 2.014324656455379 1104 32-33 0.9945483791392746 1104 34-35 0.4299559366682928 1104 36-37 1.4918741965853712 1104 38-39 4.228709286115187 1104 40-41 2.4096217388155665 1104 42-43 -1.1234680240979174 1104 44-45 0.9717194657179604 1104 46-47 1.0077613247514918 1104 48-49 -1.3558623241540246 1104 50-51 -1.4221994776673412 1104 52-53 -1.9000507509097915 1104 54-55 -0.6664444829178322 1104 56-57 -0.45345026976625746 1104 58-59 -0.07874928711096629 1104 60-61 -0.5001862564046817 1104 62-63 0.12760017788278333 1104 64-65 0.7836747279705847 1104 66-67 -2.7501369765357673 1104 68-69 3.393762673582831 1104 70-71 3.0300574950663552 1104 72-73 -1.3034497605598148 1104 74-75 0.15378877890028875 1104 76 -1.833648701863023 1105 1 1.0910544873535741 1105 2 0.8667763157894726 1105 3 0.6538423587348134 1105 4 0.32675721488965337 1105 5 -0.7480025528216885 1105 6 -0.338725603353069 1105 7 0.6530266099976529 1105 8 -2.723162896275774 1105 9 0.7840184989046506 1105 10-11 0.3617398679231556 1105 12-13 -0.8341579648941746 1105 14-15 0.5710578368004562 1105 16-17 1.0643024776854517 1105 18-19 0.08872260469284043 1105 20-21 0.23238287120018697 1105 22-23 -0.39469836872883235 1105 24-25 0.06270951016603021 1105 26-27 -0.47177474290730714 1105 28-29 0.263282424003755 1105 30-31 1.287716469320877 1105 32-33 1.6480571510691036 1105 34-35 -2.992558683214746 1105 36-37 -0.5797632303152156 1105 38-39 0.2901127948871114 1105 40-41 -0.05529054188618687 1105 42-43 -0.10592416444879404 1105 44-45 1.6091463663027596 1105 46-47 1.835246704868453 1105 48-49 -0.3690202188908671 1105 50-51 1.8029467211630674 1105 52-53 0.19790246546448031 1105 54-55 0.10694732994766554 1105 56-57 -0.41982453877211157 1105 58-59 1.5703735199065818 1105 60-61 0.47642193072982053 1105 62-63 -2.755440757789735 1105 64-65 2.1009261899588907 1105 66-67 0.7147753041659826 1105 68-69 0.31335331685768253 1105 70-71 0.9043265009143084 1105 72-73 2.0722812335922285 1105 74-75 2.9710402408885948 1105 76 2.1078717659732362 1106 1 -0.5756121793130902 1106 2 0.8001096491228026 1106 3 0.6538423587348134 1106 4 0.49342388155631767 1106 5 0.47421966940053295 1106 6 0.4279410633135967 1106 7 0.030804387775425823 1106 8 0.9435037703908904 1106 9 -1.682648167762018 1106 10-11 0.7006287568120442 1106 12-13 1.743619812883594 1106 14-15 -0.7011643854217624 1106 16-17 1.0254135887965603 1106 18-19 1.2442781602483919 1106 20-21 2.0712717600890755 1106 22-23 1.5941905201600477 1106 24-25 2.268265065721586 1106 26-27 2.6004474793149157 1106 28-29 1.8021713128926464 1106 30-31 0.8599386915430998 1106 32-33 1.298057151069095 1106 34-35 0.7352190945630355 1106 36-37 2.2480145474625672 1106 38-39 0.5901127948871157 1106 40-41 2.4780427914471446 1106 42-43 1.827409168884543 1106 44-45 2.264701921858311 1106 46-47 2.0796911493129002 1106 48-49 1.4476464477757958 1106 50-51 -0.8748310566147097 1106 52-53 2.1701246876867017 1106 54-55 0.7791695521698898 1106 56-57 1.3746199056723398 1106 58-59 1.370373519906579 1106 60-61 2.6541997085075977 1106 62-63 2.2223370199880463 1106 64-65 -1.293518254485555 1106 66-67 2.0481086374993254 1106 68-69 2.2077977613021282 1106 70-71 -0.17345127686347084 1106 72-73 -3.533274321963326 1106 74-75 -2.5900708702225153 1106 76 -6.447683789582319 1107 1 0.9410544873535756 1107 2 0.01677631578947114 1107 3 0.553842358734812 1107 4 0.49342388155631767 1107 5 0.47421966940053295 1107 6 -0.005392270019733303 1107 7 0.4974710544420944 1107 8 -0.02316289627577106 1107 9 0.9506851655713149 1107 10-11 0.37562875681204133 1107 12-13 -0.2313801871164003 1107 14-15 0.5321689479115719 1107 16-17 0.8087469221298917 1107 18-19 1.302611493581729 1107 20-21 1.4379384267557498 1107 22-23 1.0441905201600505 1107 24-25 0.9932650657215873 1107 26-27 -0.85788585401842 1107 28-29 1.4438379795593121 1107 30-31 1.7432720248764326 1107 32-33 2.5313904844024293 1107 34-35 1.1602190945630326 1107 36-37 1.7813478807958987 1107 38-39 0.39844612822044567 1107 40-41 -0.37195720855286396 1107 42-43 1.7607425022178802 1107 44-45 1.3730352551916454 1107 46-47 2.0630244826462345 1107 48-49 1.7309797811091272 1107 50-51 2.2251689433852917 1107 52-53 1.2367913543533646 1107 54-55 0.1375028855032241 1107 56-57 2.2162865723390084 1107 58-59 0.6120401865732461 1107 60-61 2.570866375174269 1107 62-63 1.0223370199880435 1107 64-65 1.6981484121811121 1107 66-67 -1.4435580291673489 1107 68-69 0.8161310946354625 1107 70-71 -0.7817846101968016 1107 72-73 -1.4416076552966572 1107 74-75 -3.1067375368891845 1107 76 1.8189828770843484 1108 1 -1.7089455126464266 1108 2 0.3167763157894754 1108 3 0.15384235873481344 1108 4 0.39342388155631625 1108 5 0.37421966940053153 1108 6 -0.05539227001973046 1108 7 -1.0525289455579028 1108 8 -1.8231628962757753 1108 9 -0.24931483442868796 1108 10-11 -0.22437124318795298 1108 12-13 0.743619812883594 1108 14-15 0.8071689479115705 1108 16-17 -0.09125307787010684 1108 18-19 1.177611493581729 1108 20-21 1.5629384267557427 1108 22-23 -0.23080947983994804 1108 24-25 0.09326506572158166 1108 26-27 -2.60788585401842 1108 28-29 0.3688379795593093 1108 30-31 -0.5817279751235631 1108 32-33 1.4063904844024293 1108 34-35 -0.23978090543696595 1108 36-37 2.1563478807958987 1108 38-39 2.4734461282204414 1108 40-41 0.6030427914471446 1108 42-43 -0.5392574977821241 1108 44-45 -0.026964744808353203 1108 46-47 1.2630244826462302 1108 48-49 1.8559797811091343 1108 50-51 0.450168943385286 1108 52-53 -1.8132086456466396 1108 54-55 -0.6874971144967716 1108 56-57 1.2662865723390055 1108 58-59 0.8620401865732461 1108 60-61 2.0958663751742606 1108 62-63 2.1473370199880435 1108 64-65 2.348148412181118 1108 66-67 2.2814419708326525 1108 68-69 -0.4838689053645382 1108 70-71 -0.256784610196803 1108 72-73 3.4333923447033428 1108 74-75 1.3182624631108162 1108 76 -0.28101712291565306 1109 1 -0.15894551264642587 1109 2 0.7667763157894711 1109 3 0.6538423587348134 1109 4 0.49342388155631767 1109 5 0.47421966940053295 1109 6 0.3946077299802653 1109 7 0.647471054442093 1109 8 0.8768371037242275 1109 9 1.1006851655713135 1109 10-11 -0.04937124318795583 1109 12-13 0.44361981288359686 1109 14-15 1.2571689479115733 1109 16-17 1.2087469221298974 1109 18-19 1.6526114935817233 1109 20-21 2.237938426755747 1109 22-23 0.9691905201600548 1109 24-25 1.0432650657215845 1109 26-27 0.8171141459815843 1109 28-29 -1.3061620204406879 1109 30-31 1.9182720248764369 1109 32-33 1.7313904844024322 1109 34-35 -0.4147809054369702 1109 36-37 1.7813478807958987 1109 38-39 4.2234461282204485 1109 40-41 4.028042791447142 1109 42-43 2.6107425022178745 1109 44-45 1.9980352551916454 1109 46-47 1.588024482646233 1109 48-49 1.8309797811091357 1109 50-51 1.7751689433852889 1109 52-53 0.8117913543533675 1109 54-55 0.8375028855032198 1109 56-57 2.041286572338997 1109 58-59 1.3870401865732447 1109 60-61 2.5458663751742634 1109 62-63 1.7723370199880435 1109 64-65 1.898148412181115 1109 66-67 0.13144197083265396 1109 68-69 -0.5088689053645403 1109 70-71 -3.106784610196801 1109 72-73 -1.76660765529666 1109 74-75 2.1682624631108176 1109 76 -0.23101712291565235 1110 1 -0.964501068201983 1110 2 -2.1887792397660846 1110 3 0.5427312476236992 1110 4 -0.2843538962214609 1110 5 0.47421966940053295 1110 6 0.21682995220248813 1110 7 0.9863599433309815 1110 8 0.9435037703908904 1110 9 1.006240721126872 1110 10-11 -0.27714902096573013 1110 12-13 -0.27860240933862457 1110 14-15 0.8766133923560133 1110 16-17 0.6476358110187803 1110 18-19 -0.1334996175293881 1110 20-21 1.7601606489779655 1110 22-23 0.43863496460449625 1110 24-25 -1.8261793787228555 1110 26-27 4.474793149071843E-4 1110 28-29 0.5410602017815336 1110 30-31 1.0099386915431054 1110 32-33 0.5925015955135464 1110 34-35 1.8407746501185898 1110 36-37 1.75357010301812 1110 38-39 1.9567794615537828 1110 40-41 0.08359834700269886 1110 42-43 -1.550368608893237 1110 44-45 1.6091463663027596 1110 46-47 1.7519133715351174 1110 48-49 2.2143131144424686 1110 50-51 0.13628005449640312 1110 52-53 -0.10765309009107682 1110 54-55 0.8847251077254441 1110 56-57 0.2746199056723384 1110 58-59 -0.1796264800934253 1110 60-61 -0.051355847047958036 1110 62-63 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-1.1000453759527744 2116 70-71 3.852038919214962 2116 72-73 6.815745285879814 2116 74-75 1.0314977572284612 2116 76 -0.4280759464450661 2117 1 1.0910544873535741 2117 2 0.7334429824561397 2117 3 0.6538423587348134 2117 4 0.4267572148896477 2117 5 0.47421966940053295 2117 6 -0.03872560335306474 2117 7 0.897471054442093 2117 8 0.21017043705755611 2117 9 -2.2159815010953494 2117 10-11 -0.9993712431879587 2117 12-13 -2.689713520449736 2117 14-15 -0.734497718755101 2117 16-17 -3.6079197445367726 2117 18-19 -1.6890551730849381 2117 20-21 0.5046050934224127 2117 22-23 0.39419052016005196 2117 24-25 -1.3317349342784155 2117 26-27 -1.4995525206850928 2117 28-29 -0.19782868710735357 2117 30-31 -3.3733946417902345 2117 32-33 -4.835276182264231 2117 34-35 -2.864780905436966 2117 36-37 -2.985318785870767 2117 38-39 -0.5765538717795557 2117 40-41 1.3113761247804732 2117 42-43 -0.8059241644487898 2117 44-45 -2.035298078141686 2117 46-47 -2.7536421840204355 2117 48-49 -2.0190202188908657 2117 50-51 -1.2748310566147083 2117 52-53 0.27012468768670317 2117 54-55 -1.1874971144967716 2117 56-57 -1.6253800943276602 2117 58-59 -2.7962931467600853 2117 60-61 -1.4124669581590652 2117 62-63 0.9223370199880492 2117 64-65 0.17314841218111354 2117 66-67 -0.6852246958340089 2117 68-69 -0.19220223869787034 2117 70-71 -2.2401179435301337 2117 72-73 0.5000590113700092 2117 74-75 3.2765957964441483 2117 76 0.28564954375101337 2118 1 -0.708945512646423 2118 2 0.8001096491228026 2118 3 -0.4794909745985194 2118 4 0.2934238815563148 2118 5 0.47421966940053295 2118 6 0.4279410633135967 2118 7 1.0308043877754258 2118 8 1.0768371037242233 2118 9 -3.4159815010953523 2118 10-11 -0.9993712431879587 2118 12-13 -1.4563801871164017 2118 14-15 1.2321689479115676 2118 16-17 -2.907919744536777 2118 18-19 -0.889055173084941 2118 20-21 -1.2953949065775916 2118 22-23 -1.0058094798399466 2118 24-25 0.9682650657215817 2118 26-27 -3.899552520685088 2118 28-29 -0.7978286871073479 2118 30-31 -1.7400613084568946 2118 32-33 -1.5352761822642336 2118 34-35 -0.9981142387702988 2118 36-37 -3.9186521192041006 2118 38-39 -2.1432205384462186 2118 40-41 -1.8219572085528597 2118 42-43 0.3274091688845431 2118 44-45 -4.668631411475019 2118 46-47 -0.48697551735376976 2118 48-49 0.4143131144424643 2118 50-51 -0.47483105661471114 2118 52-53 -2.6965419789799654 2118 54-55 -1.3874971144967745 2118 56-57 0.7079532390056684 2118 58-59 0.7037068532399147 2118 60-61 -3.9124669581590688 2118 62-63 -1.7443296466786222 2118 64-65 1.8398150788477778 2118 66-67 1.881441970832654 2118 68-69 -2.325535572031203 2118 70-71 -1.6067846101968009 2118 72-73 2.80005901137001 2118 74-75 -1.923404203555851 2118 76 -0.8476837895823195 2119 1 0.7577211540202384 2119 2 -3.3332236842105267 2119 3 -1.7461576412651851 2119 4 0.026757214889649106 2119 5 -0.3924469972661342 2119 6 0.29460772998026385 2119 7 -1.7025289455579014 2119 8 0.2768371037242261 2119 9 -0.01598150109535368 2119 10-11 0.20062875681204417 2119 12-13 1.0769531462169368 2119 14-15 -0.9344977187550967 2119 16-17 -2.6745864112034425 2119 18-19 -0.38905517308493387 2119 20-21 -4.528728239910922 2119 22-23 -1.105809479839948 2119 24-25 0.6682650657215845 2119 26-27 0.6671141459815786 2119 28-29 0.10217131289264358 2119 30-31 -0.7067279751235631 2119 32-33 -1.568609515597565 2119 34-35 -1.8314475721036345 2119 36-37 -1.551985452537437 2119 38-39 -2.043220538446217 2119 40-41 -4.655290541886192 2119 42-43 -4.30592416444879 2119 44-45 0.0980352551916468 2119 46-47 -1.9869755173537698 2119 48-49 -1.3523535522242014 2119 50-51 -1.4414977232813797 2119 52-53 -1.8632086456466297 2119 54-55 -0.1541637811634402 2119 56-57 -4.992046760994331 2119 58-59 -2.5629598134267546 2119 60-61 -2.7458002914924045 2119 62-63 -0.44432964667862507 2119 64-65 -1.4601849211522193 2119 66-67 -1.3852246958340118 2119 68-69 -1.8255355720312032 2119 70-71 -1.2067846101968023 2119 72-73 -2.7332743219633215 2119 74-75 -1.6900708702225167 2119 76 -1.3810171229156545 >>END_MODULE >>Per sequence quality scores pass #Quality Count 22 4.0 23 8.0 24 13.0 25 26.0 26 62.0 27 99.0 28 175.0 29 242.0 30 372.0 31 543.0 32 727.0 33 990.0 34 1362.0 35 1266.0 36 438.0 37 214.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 4.1430973857208375 17.50496865922642 12.643326708454364 65.70860724659838 2 1.9110227793915302 4.204250114661367 81.3178413086684 12.566885797278704 3 2.277939153034704 18.666870509096466 68.33817459104112 10.717015746827702 4 1.9415991438617948 55.90888243387862 23.131019721755084 19.01849870050451 5 1.4523773123375632 30.515211741323956 62.94144626203945 5.090964684299037 6 3.715028283137135 64.71487540131479 10.56413392447638 21.0059623910717 7 3.7456046476073994 29.888396269683536 9.753860266014371 56.61213881669469 8 2.507261886561688 64.71487540131479 17.10747592111298 15.670386791010548 9 2.828313713499465 14.600214034551293 5.014523773123376 77.55694847882587 10-11 3.294603271670998 36.12597462161749 38.47271059471029 22.106711512001223 12-13 10.42654028436019 7.093716557101361 38.35804922794679 44.121693930591654 14-15 13.04846353768537 11.099220302706009 10.571778015593946 65.28053814401467 16-17 35.85078734138511 31.080874484023852 2.7442287112062376 30.3241094633848 18-19 36.69163736431738 18.39168322886409 2.5378382510319524 42.37884115578658 20-21 14.68429903684452 6.765020639046017 2.4766855220914232 76.07399480201803 22-23 12.964378535392143 41.46919431279621 2.7289405289711053 42.837486622840544 24-25 36.095398257147224 42.41706161137441 2.9353309891453905 18.552209142332977 26-27 43.20440299648372 46.575447179330375 3.8831982877235895 6.3369515364623155 28-29 10.189573459715639 45.65051215410488 11.221525760587065 32.93838862559242 30-31 40.40666564745452 20.799571930897418 30.973857208377925 7.819905213270142 32-33 34.000917290934105 36.02660143708913 25.745298883962697 4.227182388014064 34-35 10.824033022473628 44.4045253019416 40.60541201651124 4.166029659073536 36-37 3.5468582785506806 34.51307139581104 50.298119553585074 11.641950772053203 38-39 9.723283901544107 13.017887173215104 37.822962849717165 39.43586607552362 40-41 6.466901085460938 10.732303929062835 44.52683075982266 38.273964225653565 42-43 39.51230698669928 5.32028741782602 43.06680935636753 12.100596239107169 44-45 8.981807063140193 31.837639504662896 39.940376089282985 19.240177342913928 46-47 40.07032563828161 6.512765632166335 19.37012689191255 34.04678183763951 48-49 33.61106864393824 3.13407735820211 14.095704020791928 49.15914997706773 50-51 10.304234826479131 3.5086378229628497 44.37394893747133 41.81317841308668 52-53 9.157621158844213 3.7073841920195685 72.3131019721755 14.82189267696071 54-55 4.976303317535545 3.6997401009020026 50.152881822351326 41.17107475921113 56-57 38.49564286806299 4.059012383427611 22.09142332976609 35.35392141874331 58-59 8.607246598379453 19.446567803088215 60.22779391530347 11.718391683228864 60-61 31.654181317841307 48.85338633236508 15.066503592722825 4.425928757070785 62-63 5.778932884879988 86.70692554655251 2.866534169087296 4.647607399480202 64-65 1.8498700504510013 90.16969882280996 2.851245986852163 5.129185139886867 66-67 2.140345512918514 88.05992967436171 2.958263262498089 6.841461550221678 68-69 2.446109157621159 83.04540590123834 3.768536920960098 10.739948020180401 70-71 3.5621464607858124 76.32624980889771 5.297355144473323 14.814248585843142 72-73 5.04510013759364 61.26739030729246 10.074912092952149 23.612597462161748 74-75 5.38144014676655 58.70661978290781 10.258370279773734 25.653569790551906 76 4.341843754777557 59.8379452683076 10.09020027518728 25.73001070172756 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.5 17 1.0 18 0.5 19 0.0 20 0.0 21 0.0 22 0.0 23 2.5 24 3.0 25 1.0 26 0.5 27 1.5 28 6.5 29 10.0 30 8.0 31 5.0 32 5.0 33 6.0 34 14.5 35 30.0 36 106.5 37 176.0 38 538.5 39 1237.5 40 1503.0 41 1174.0 42 916.0 43 720.5 44 398.0 45 214.5 46 158.0 47 125.5 48 73.5 49 43.0 50 32.0 51 25.5 52 19.0 53 13.5 54 8.0 55 19.0 56 29.5 57 24.0 58 19.0 59 24.0 60 29.0 61 30.5 62 32.0 63 35.0 64 27.0 65 18.5 66 14.5 67 8.0 68 7.5 69 5.5 70 3.0 71 2.0 72 2.5 73 1.5 74 0.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 6541.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 20.562605106252864 #Duplication Level Percentage of deduplicated Percentage of total 1 73.82899628252788 15.181164959486319 2 12.565055762081784 5.167405595474698 3 4.907063197026022 3.0270600825561838 4 1.858736059479554 1.5288182235132242 5 1.7100371747211895 1.758140957040208 6 1.1895910780669146 1.4676654945726952 7 0.37174721189591076 0.5350863782296285 8 0.37174721189591076 0.6115272894052898 9 0.22304832713754646 0.41278092034857056 >10 2.2304832713754648 6.971411099220302 >50 0.37174721189591076 5.626051062528665 >100 0.22304832713754646 11.17566121388167 >500 0.07434944237918216 10.212505733068339 >1k 0.07434944237918216 36.32472099067421 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGT 2376 36.32472099067421 No Hit ATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGTCTT 668 10.212505733068339 Illumina PCR Primer Index 10 (95% over 23bp) TCTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCG 340 5.197981959944962 No Hit CTCTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCC 286 4.372420119247821 No Hit CTTTACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGTC 105 1.6052591346888856 Illumina PCR Primer Index 10 (95% over 21bp) CTTATACACATCTCCGAGCCCACGAGACAGTAGATTATATCGTATGCCGT 98 1.4982418590429598 No Hit TATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGTCT 81 1.2383427610457116 Illumina PCR Primer Index 10 (95% over 22bp) CTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGTC 74 1.131325485399786 Illumina PCR Primer Index 10 (95% over 21bp) CTTATACACATCTCCGAGCCCACGAGACAGTAGATTAACTCGTATGCCGT 60 0.9172909341079345 No Hit CTTATACACATCTCCGAGCCCACGAGACAGTAGATTACCTCGTATGCCGT 55 0.8408500229322734 No Hit TCTTATACACATCTCCGAGCCCACGAGACAGTAGATTAACTCGTATGCCG 27 0.41278092034857056 No Hit CTTATACACATCTCCGAGCCCACGAGACAGTAGCTTATCTCGTATGCCGT 26 0.3974927381134383 TruSeq Adapter, Index 10 (95% over 23bp) ATACACATCTCCGAGCCCACGAGACAGTAGATTAACTCGTATGCCGTCTT 25 0.38220455587830604 No Hit ACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGTCTTCT 23 0.3516281914080416 Illumina PCR Primer Index 10 (96% over 25bp) ATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGTCTTCTGCTT 20 0.3057636447026449 Illumina PCR Primer Index 10 (96% over 29bp) TCTTAATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCC 19 0.2904754624675126 No Hit CTTATACACATCTCCGAGCCCACGAGCCAGTAGATTATCTCGTATGCCGT 19 0.2904754624675126 No Hit CTTATACACATCTCCGAGCCCACGCGACAGTAGATTATCTCGTATGCCGT 17 0.25989909799724814 No Hit CTCTTATACACATCTCCGAGCCCACGAGACAGTAGATTATATCGTATGCC 17 0.25989909799724814 No Hit CTTATACACATCTCCGAGCCCACGAGACAGTAGATTAGCTCGTATGCCGT 16 0.24461091576211588 No Hit TCTTATACACATCTCCGAGCCCACGAGACAGTAGATTATATCGTATGCCG 16 0.24461091576211588 No Hit ATACACATCTCCGAGCCCACGAGACAGTAGATTACCTCGTATGCCGTCTT 15 0.22932273352698362 No Hit CTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTAGTATGCCGT 15 0.22932273352698362 No Hit CTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCAGT 15 0.22932273352698362 No Hit CTTATACACATCTCCGAGCCCACGAGACCGTAGATTATCTCGTATGCCGT 14 0.21403455129185142 No Hit TTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGTC 14 0.21403455129185142 Illumina PCR Primer Index 10 (95% over 21bp) CTTATACACATCTACGAGCCCACGAGACAGTAGATTATCTCGTATGCCGT 13 0.19874636905671916 No Hit CTCTTATACACATCTCCGAGCCCACGAGACAGTAGATTAACTCGTATGCC 13 0.19874636905671916 No Hit ATACACATCTCCGAGCCCACGAGACAGTAGATTATATCGTATGCCGTCTT 13 0.19874636905671916 No Hit CTTATACACATCTCCGAGCCCAAGAGACAGTAGATTATCTCGTATGCCGT 12 0.1834581868215869 No Hit CTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTCTGCCGT 12 0.1834581868215869 No Hit CTTATACACATCTCCGAGCACACGAGACAGTAGATTATCTCGTATGCCGT 12 0.1834581868215869 No Hit CTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGC 11 0.16817000458645467 No Hit CTTATACACATCTCCGAGCCCACGAGAAAGTAGATTATCTCGTATGCCGT 11 0.16817000458645467 No Hit TCTTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCC 11 0.16817000458645467 No Hit CTTATACACATCTGACGCCTGTCTCTTATACACATCTCCGAGCCCACGAG 10 0.15288182235132244 No Hit CTTATACACATCTCCGAGCCCACGAGACAGTAGATTATTTCGTATGCCGT 10 0.15288182235132244 No Hit ATTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGT 10 0.15288182235132244 No Hit CTTATACACATCTCCGAGCCCACGTGACAGTAGATTATCTCGTATGCCGT 10 0.15288182235132244 No Hit CTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGACGT 10 0.15288182235132244 No Hit CTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCCCGTATGCCGT 9 0.13759364011619019 No Hit CTTATCACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGTC 9 0.13759364011619019 Illumina PCR Primer Index 10 (95% over 21bp) CTTATACACATATCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGT 9 0.13759364011619019 No Hit TCTTATACACATCTCCGAGCCCACGAGACAGTAGATTACCTCGTATGCCG 8 0.12230545788105794 No Hit CTCTTATACACATCTCCGAGCCCACGAGACAGTAGATTACCTCGTATGCC 8 0.12230545788105794 No Hit CTTATTACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCG 8 0.12230545788105794 No Hit GACAACATTCTTTTACTTCAAATGCTTGACATAGTGCTTTCCTTTAACTG 8 0.12230545788105794 No Hit CTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCACGTATGCCGT 8 0.12230545788105794 No Hit TTCACAGACTGACTGGGAATTTAGGGAATATAAGGAATCTGTCTCTTATA 7 0.10701727564592571 No Hit CTTATACACATCTCCGAGCCCACGAGACAGTAGATTACATCGTATGCCGT 7 0.10701727564592571 No Hit CTTATACACATCTCCGAGCCCACGAGACAGTAGATCATCTCGTATGCCGT 7 0.10701727564592571 No Hit CTCTTATACACATCTCCGAGCCCACGAGACAGTAGATTCTCTCGTATGCC 7 0.10701727564592571 No Hit ATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGTCCT 7 0.10701727564592571 Illumina PCR Primer Index 10 (95% over 21bp) >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.07644091117566121 0.0 2 0.0 0.0 0.0 0.07644091117566121 0.0 3 0.0 0.0 0.0 0.07644091117566121 0.0 4 0.0 0.0 0.0 0.07644091117566121 0.0 5 0.0 0.0 0.0 0.07644091117566121 0.0 6 0.0 0.0 0.0 0.07644091117566121 0.0 7 0.0 0.0 0.0 0.07644091117566121 0.0 8 0.0 0.0 0.0 0.1070172756459257 0.0 9 0.0 0.0 0.0 0.1070172756459257 0.0 10 0.0 0.0 0.0 0.1070172756459257 0.0 11 0.0 0.0 0.0 0.13759364011619019 0.0 12 0.0 0.0 0.0 0.1834581868215869 0.0 13 0.0 0.0 0.0 0.1834581868215869 0.0 14 0.0 0.0 0.0 0.1834581868215869 0.0 15 0.0 0.0 0.0 0.1834581868215869 0.0 16 0.0 0.0 0.0 0.1834581868215869 0.0 17 0.0 0.0 0.0 0.1834581868215869 0.0 18 0.0 0.0 0.0 0.30576364470264483 0.0 19 0.0 0.0 0.0 0.6115272894052897 0.0 20 0.0 0.0 0.0 0.6573918361106864 0.0 21 0.0 0.0 0.0 0.7796972939917444 0.0 22 0.0 0.0 0.0 0.7796972939917444 0.0 23 0.0 0.0 0.0 0.7796972939917444 0.0 24 0.0 0.0 0.0 0.7796972939917444 0.0 25 0.0 0.0 0.0 0.7796972939917444 0.0 26 0.0 0.0 0.0 0.7796972939917444 0.0 27 0.0 0.0 0.0 0.7949854762268767 0.0 28 0.0 0.0 0.0 0.8408500229322734 0.0 29 0.0 0.0 0.0 0.8714263874025379 0.0 30 0.0 0.0 0.0 0.8714263874025379 0.0 31 0.0 0.0 0.0 1.009020027518728 0.0 32 0.0 0.0 0.0 1.1007491209295215 0.0 33 0.0 0.0 0.0 1.131325485399786 0.0 34 0.0 0.0 0.0 1.2077663965754473 0.0 35 0.0 0.0 0.0 1.253630943280844 0.0 36 0.0 0.0 0.0 1.4982418590429598 0.0 37 0.0 0.0 0.0 1.6511236813942822 0.0 38 0.0 0.0 0.0 1.9874636905671916 0.0 39 0.0 0.0 0.0 2.24736278856444 0.0 40 0.0 0.0 0.0 2.4002446109157622 0.0 41 0.0 0.0 0.0 2.7518728023238035 0.0 42 0.0 0.0 0.0 3.103500993731845 0.0 43 0.0 0.0 0.0 3.286959180553432 0.0 44 0.0 0.0 0.0 3.5315700963155483 0.0 45 0.0 0.0 0.0 3.6691637364317384 0.0 46 0.0 0.0 0.0 3.8679101054884573 0.0 47 0.0 0.0 0.0 4.112521021250573 0.0 48 0.0 0.0 0.0 4.234826479131631 0.0 49 0.0 0.0 0.0 4.372420119247821 0.0 50 0.0 0.0 0.0 4.678183763950466 0.0 51 0.0 0.0 0.0 4.785201039596392 0.0 52 0.0 0.0 0.0 4.892218315242317 0.0 53 0.0 0.0 0.0 5.060388319828772 0.0 54 0.0 0.0 0.0 5.136829231004434 0.0 55 0.0 0.0 0.0 5.18269377770983 0.0 56 0.0 0.0 0.0 5.503745604647607 0.0 57 0.0 0.0 0.0 5.763644702644855 0.0 58 0.0 0.0 0.0 6.008255618406971 0.0 59 0.0 0.0 0.0 6.1152728940528975 0.0 60 0.0 0.0 0.0 6.298731080874484 0.0 61 0.0 0.0 0.0 6.329307445344749 0.0 62 0.0 0.0 0.0 6.482189267696071 0.0 63 0.0 0.0 0.0 6.573918361106864 0.0 64 0.0 0.0 0.0 6.696223818987923 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTGCAAA 15 0.0021264292 70.00001 58 CTATACA 15 0.0021264292 70.00001 1 AGCCAGT 15 0.0021264292 70.00001 25 TTACCTC 15 0.0021264292 70.00001 35 CCAGTAG 15 0.0021264292 70.00001 27 TGCAAAA 15 0.0021264292 70.00001 59 GAGCCAG 15 0.0021264292 70.00001 24 CACGAGC 15 0.0021264292 70.00001 21 GCCAGTA 15 0.0021264292 70.00001 26 ATTACCT 15 0.0021264292 70.00001 34 GCAAAAA 15 0.0021264292 70.00001 60 TGCTTGC 15 0.0021264292 70.00001 55 GCTTGCA 15 0.0021264292 70.00001 56 ACCTCGT 15 0.0021264292 70.00001 37 CTTGCAA 15 0.0021264292 70.00001 57 GATTACC 15 0.0021264292 70.00001 33 TACCTCG 15 0.0021264292 70.00001 36 AGATTAC 15 0.0021264292 70.00001 32 CCTCGTA 20 0.0066466313 52.5 38 ACGAGCC 20 0.0066466313 52.5 22 >>END_MODULE