FastQCFastQC Report
Wed 25 May 2016
SRR1780553_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780553_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences70
Sequences flagged as poor quality0
Sequence length50
%GC58

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAAGGGCCGGAGCGTCGTGATCGCCGACTCCGGCACGTTCGGCGGCACC11.4285714285714286No Hit
GGTACAGCACGTGGAGCTCGCCGGCGGCCCGCTCGCGCCCTGTCTCTTAT11.4285714285714286No Hit
TCACCCACTAGGATACCAACAAACCTACCCATCTTTAACAGTACATAGTA11.4285714285714286No Hit
GCCGGATGTCAGAGGGGTGCCTTGGGTCTGTCTCTTATACACATCTGACG11.4285714285714286No Hit
CCGCACGGGCGCCCTTGACGGCCAGGTCCTTGTCCTGGCTGCGGGCTGTC11.4285714285714286No Hit
GAGTTCGGCCCCGCCCGGGCCGGCCTCGTGGCCGACTCCCTGCACCTGCC11.4285714285714286No Hit
CGCCAGGACCGCGTGATGACGCGCATGACCTGGGCGCTGTCTCTTATACA11.4285714285714286No Hit
GGACAGGGAGAGCACGACGGCGGCCACGCGCGGGTGTTCGACTGTCTCTT11.4285714285714286No Hit
GGCTTGAACAGCTCGAGCTGGAGGATCTCGGCGCGCTTCTTCTCGCCGCC11.4285714285714286No Hit
TGATGTAGGCGCGGTACCGCTCGGTGGCGAGGCGGGGGTCGGGCAGGGGG11.4285714285714286No Hit
GGCCTCGTGCGCTCGGCCGCCCAGGAAGGAGCCTGTCTCTTATACACATC11.4285714285714286No Hit
GGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGCAAG11.4285714285714286No Hit
GTGGTGGCGATGTCCGCGGTCACCGTGCGGGCGGCCATGCCTGTCTCTTA11.4285714285714286No Hit
AGGGTCGTCATCATAAAGGGAGAAATGTGGAATTTATTCATAAGCAAAGA11.4285714285714286No Hit
CGGCTCATCCTAATGACCATAATAACGCAAACAGCATACTCATTCATCTT11.4285714285714286No Hit
TCTTATACACATCTGACGCTGCCGCCGAATGACCCTCAGGAGGACCTCTG11.4285714285714286No Hit
GAGCGGTCTTAATCAAATTGCCTCCCGAGTATTGGCACTTTCCTTTGCTT11.4285714285714286No Hit
GACCAAACCTATTTGTTTATGGGGTGATGTGAGCCCGTCTAAACATTTTC11.4285714285714286No Hit
GAATATTGAGGCGCCATTGGCGTGAAGGTAGCGGATGATTCAGCCATAAT11.4285714285714286No Hit
CCAGTCTTCACCTCCTAGGATAACTCCCTGCGGGCCGAACCACGTACCTC11.4285714285714286No Hit
TCCACAACCTTAACAATGAACAAGATATTCGAAAAATAGGAGGACTACTC11.4285714285714286No Hit
ATCTTGGCAAAATAAATCTCAACGCTGACTGAGACTGCCTCCGTCATTCT11.4285714285714286No Hit
CCCTTAGTCCTTCTGATCTCAGCTTGAAGTCACCTCCTCCAGGAAGCCCT11.4285714285714286No Hit
GTTTTGATGTGGATTGGGTTTTTATGTACTACAGGTGGTCAAGTATTTAT11.4285714285714286No Hit
GCCCACCACGACGCCGGCTGAGATCCGGCCCTCCACCATCGAATGCCCCA11.4285714285714286No Hit
GGCCGGGGGCGGGATGGTCCCGGGAGCGGTGCCCGAGGCTGTCTCTTATA11.4285714285714286No Hit
CGCGTCGAAGGCGCCTCCCAGCCGGGGCTCAGTCGCTGTCTCTTATACAC11.4285714285714286No Hit
CTGTCCGCCCTCCCTCTAGATGGAAACGTTTCCCTTTCTGCTTCAAAACC11.4285714285714286No Hit
GCAAGACGAGAAGACCCTATGGAGCTTTAATTTATTAATGCAAACAGTAC11.4285714285714286No Hit
ATACAAATCTGACGCTGCCGACGAATGACCATGTGTAGAACTCGGGGGTC11.4285714285714286No Hit
CTGTCTCTTATACACATCTGACGCTGCCGACGAGCCGTGCCGTTGAAAGG11.4285714285714286No Hit
GAGTGTGCCGTCTGAGGCTGCCGCCGGGACGACCGGTGTGTGCCCCTCTG11.4285714285714286No Hit
CAAGTATACTTCAAAGGACATTTAACTAAAACCCCTACGCATTTATATAG11.4285714285714286No Hit
CAGTAATTCCACCCTTACTCTCCGCCGTCCCCTGCGTTGGGACAGGCCTA11.4285714285714286No Hit
GGATTACGGGGACGGGTCCCCCACCAAGACCACCTCAGTCGCTGTCTCTT11.4285714285714286No Hit
CTAAAGGACGAACCTGATCTCTTATACTAGTATCCTTAATCATTTTTATT11.4285714285714286No Hit
ATAATGATGTATGCTTTGTTTCTGTTGAGTGTGGGTTTAGTAATGGGGCT11.4285714285714286No Hit
GCTGCGCGCCCACCAGGTCCACGTCCGTGACCTCCTCGGTCACGGTGCTG11.4285714285714286No Hit
CCCCAAGGAATGGTGTTTGATCAAATGAGATCTAATTCCATTTGTTCAGT11.4285714285714286No Hit
CTCGTCGTCCGCGGCGCCGGCCGCCGTGTACACCCGCGCCGCGAGCCCGG11.4285714285714286No Hit
GCGTCAGGACGAGCGAGCGCTGCTTCGTGATCTTCGTGAAGCTGTCTCTT11.4285714285714286No Hit
GCATCCCGTGTTCGACATCCTCGTCGCCCTGTTCGTCCTGTCTCTTATAC11.4285714285714286No Hit
GCTCTCATTGCCCTCAGCTTCTGTTCAGCTGCCAAGTGCCAGCGTTCCCT11.4285714285714286No Hit
GCCGTGTGGTGGGCGACGACGTCGCCATCATGATCTGTCTCTTATACACA11.4285714285714286No Hit
GGTGCGCCTGGGGGCCCGCTCCGGCTCGGCCCCGTGCTCGGCGGGGGACC11.4285714285714286No Hit
ACGTCGAGTCGGACGGCGCCAAGCCCCTCGACCTCGACCTGTCTCTTATA11.4285714285714286No Hit
CCGGGAGACCGTGGACCTGATGCTGCGCGTCTTCCCGGTCCGCACGTGCT11.4285714285714286No Hit
ACTGGAAAGTGCACTTGGACGAACCAGAGTGTAGCTTAACACAAAGCACC11.4285714285714286No Hit
ACACATAATGACCCACCAATCACATGCCTATCATATAGTAAAACCCAGCC11.4285714285714286No Hit
GGCGGAGAGGGCACGGGCGGTCTGGATGGAGGAGGCGCGCGGGTCGGGGT11.4285714285714286No Hit
CTTTTTGGCTGCTCCCCCCGCTGTCCCCCCGCCCCCCCCTGTCCCTTCAA11.4285714285714286No Hit
CACATGAACAGCTGGGCCACGAGGTACATGGCCAGCACGATCAGGATCCT11.4285714285714286No Hit
GCCAATCTCCAACCAACGCAAGGAAACCGTGAGGACCGGTCCAACCGCCT11.4285714285714286No Hit
GCGCCCGGACGAGTTCCGCCTGTACGAGCTGATCTGGAAGCGCACCGTGG11.4285714285714286No Hit
GTCCAGGCGGTCGCCGGTGACGAGCGTCTGCACGGCCCTGTCTCTTATAC11.4285714285714286No Hit
GTCCAGCAAGGACGCGGGAAGCGGGGTGGGGCGACGCATGGTGCCAGCCT11.4285714285714286No Hit
ATGGGGAGTAGTGTGATTAAGGTGGAGTAGATTAGGCGTAGGTAGAAGTA11.4285714285714286No Hit
GGGCACACCCGCGCCGAGCTCGCCGAGCTGGCCGCCCCGCTGGGCGCGGA11.4285714285714286No Hit
ACTGCGAGCAGTAGCCCAAACAATCTCATATGAAGTCACCCTAGCCATCA11.4285714285714286No Hit
GATATATAAACTCAGACCCAAACATTAATCAGTTCTTCAAATATCTACTC11.4285714285714286No Hit
CAGCTGCACATCCGTCTCGAGGTTCGCCGTGGGGAAGCCGAGCTCGCGGC11.4285714285714286No Hit
GCGTGGTGGAGACGGACCGGGGCCTCGGGGTCCTCACCGAGCCTGTCTCT11.4285714285714286No Hit
CACGTGCACGACCAGCGCCGAGAGGAACCGACGGGAGACGGGGGAGTAGG11.4285714285714286No Hit
CTGCGCATCGTCTCCGCCGGCCTGCGCGCCGGGGCGCCCATGACCGTGAG11.4285714285714286No Hit
GACTAATCTTCAACTCCTACATACTTCCCCCATTATTCCTGGAACCAGGC11.4285714285714286No Hit
CTAGTCAACGGTTGGCGAACATCAGTGGGGGTGGGGTACAACGGCTGTGT11.4285714285714286No Hit
GCGTGGCGGAACGGCGGGGGGGGGCGGGCCCCGCGGCGCCCCCCCCGGCC11.4285714285714286No Hit
ATCTCACCGCGGGCCGTCGTCGGGACCACGGAGGCGAAGGCTGTCTCTTA11.4285714285714286No Hit
GTGTCGGACTGGCACCCAGAATCTGTCTTCAGCCCGGGCCTCGTGCCTAG11.4285714285714286No Hit
GTCTTGGCCCCGTCGATCGCCGGCCCATCGCCGAAGTCGTACTGCAGCCA11.4285714285714286No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGGGT50.044.021
GCGGGAA50.044.014
CAGCAAG50.044.04
GCAAGGA50.044.06
CGGGGTG50.044.022
GCGACGC50.044.031
CCAGCCT50.044.044
CCAGCAA50.044.03
ACGCATG50.044.034
AGGACGC50.044.09
GAAGCGG50.044.018
ATGGTGC50.044.038
GGCGACG50.044.030
TCCAGCA50.044.02
GCCAGCC50.044.043
TGGGGCG50.044.027
GTGGGGC50.044.026
GACGCGG50.044.011
GACGCAT50.044.033
AGCGGGG50.044.020
Produced by FastQC (version 0.11.5)