FastQCFastQC Report
Wed 25 May 2016
SRR1780553_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780553_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences70
Sequences flagged as poor quality0
Sequence length50
%GC60

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCATGGCCGCCCGCACGGTGACCGCGGACATCGCCACCACCTGTCTCTTA11.4285714285714286No Hit
CCCCTGCGTCCCCGCCTGCACAGACCCCCGCGCACCCGAACTCCTCCCCA11.4285714285714286No Hit
GGACATCGCGGACTCGCTGCCCGAGGGCGGCGCGCAGTCGCCCGCGATGA11.4285714285714286No Hit
GCCCAGGACGCCTCCGCCCTCGAGGGGGTCGCCCGAGAGGTGGCCGCCGA11.4285714285714286No Hit
TGATGACGGTGCCCGAGGCGCTGAACTGCGCGAGGCGGCCGTCCTGCGCG11.4285714285714286No Hit
ACCCTAACATAACCATTCTTAATTTAACTATTTATATTATCCTGACTACT11.4285714285714286No Hit
CGCACGGCCTCGGCCGAGAGCCCGTCCGCATCGGTCCCCTCCACGGTGGG11.4285714285714286No Hit
GCATTGCTGCGTGCTTGATGCTTGTCCCTTTTGATCGTGGTGATTTAGAG11.4285714285714286No Hit
ATCTCCGAGCCCACGAGAATGATGATATCTCGTATGCCGTCTTCTGCTTG11.4285714285714286TruSeq Adapter, Index 25 (96% over 29bp)
GCCCTGGATCACCGAGCCGGGCGCATGGCTGCAGTACGACTTCGGCGATG11.4285714285714286No Hit
AAGTAAGAGATACACCAGCATGGGTTGGCTGATTACTTGTCTACATGGCT11.4285714285714286No Hit
CTGCGGCAGATCACCTATATTGCACTTGGGCAGAAGGGAGGGCTTCCTGG11.4285714285714286No Hit
TGCACCCCCTGCACGCGATCGAGCCAGGCCCCCATCCGGCGGACTCCCCC11.4285714285714286No Hit
ATGCAGGAGGCCGCGCTCGCCGTGATCGACCCCGACCCGCGCGCCTCCTC11.4285714285714286No Hit
TCCTACGCCCTCTCAGCCGATGAACAGTTGGAATAGGTTATTAGCGGTAA11.4285714285714286No Hit
CCCGCAGCCAGGACAAGGACCTGGCCGTCAAGGGCGCCCGTGCGGCTGTC11.4285714285714286No Hit
CTGAAGGGCACGTCCGCGCCCAGCGGGGCGGCCAGCTCGGCGAGCTCGGC11.4285714285714286No Hit
ACCCTCGAGTTCGCGGACCAGTCCCCGGAGGAGTTCGTCCGCGGCATGCT11.4285714285714286No Hit
CACCGTGACCGAGGAGGTCACGGACGTGGACCTGGTGGGCGCGCAGCCTG11.4285714285714286No Hit
GGTCCTCTCGTCTCCGAGCCCACGAGACAATTCGTTATCTCGTATGCCGT11.4285714285714286No Hit
ATACATCATTATTCTCGCACGGGCTACAACCACGACCAATGATATGAAAA11.4285714285714286No Hit
TTCATGAACCGCAACGTCAACGAGGGCTTCTCCGGCGGCGAGAAGAAGCG11.4285714285714286No Hit
GCTTTGGTTCTCCTTGCAAAGTTATTTCTAGTTAATTCATTATGCAGAAG11.4285714285714286No Hit
CGACTGAGCCCCGGCTGGGAGGCGCCTTCGACGCGCTGTCTCTTATACAC11.4285714285714286No Hit
ACCTAGGCACGGGGCCGGGGCGGTCGACAAGGTCTGGGGGTCAGTCCGGC11.4285714285714286No Hit
GCTCGGTGAGGACCCCGGGGCCCCGGTCCGTCTCCACCACGCCTGTCTCT11.4285714285714286No Hit
CTCTTATACACATCTCCGAGCCCACGCATGATGATATCTCGTATGCCGTC11.4285714285714286No Hit
CTTATACACATCTCCGAGCCCACGAGCGATCCAAAATCTCGTATGCCGTC11.4285714285714286No Hit
CCGGGGTGCGCATCGGCGACGCGGACCGGGTGCGCCTGGGCGCCCACCTG11.4285714285714286No Hit
CAGTTTATGTAGCTTACCTCCTCAAAGCAATACACTGAAAATGTTTAGAC11.4285714285714286No Hit
GTCGAGGTCGAGGGGCTTGGCGCCGTCCGACTCGACGTCTGTCTCTTATA11.4285714285714286No Hit
GGCGCGAGCGGGCCGCCGGCGAGCTCCACGTGCTGTACCCTGTCTCTTAT11.4285714285714286No Hit
CTGTCTCTTATACACATCCCGAGCCCACGAGACATGATGATATCTCGTAT11.4285714285714286No Hit
GGAACTCCGCCACCGCCAGCAGCCGTCACAGCAACATCAAACACGGGTGT11.4285714285714286No Hit
CCTTCGCCTCCGTGGTCCCGACGACGGCCCGCGGTGAGATCTGTCTCTTA11.4285714285714286No Hit
GTGCTGGACCCCCGCCTGGCCACCGAGCGGTACGGCGGCTACAACCCTGT11.4285714285714286No Hit
GAACCCAACCTCCGAGCCCACGAGACATGATGATATCTCGTATGCCGTCT11.4285714285714286RNA PCR Primer, Index 25 (95% over 21bp)
GCACTCTTGTGCGGGATATTGATTTCACGGAGGATGGTGGTCAAGGGACC11.4285714285714286No Hit
ATCATGATGGCGACGTCGTCGCCCACCACACGGCCTGTCTCTTATACACA11.4285714285714286No Hit
CGTGGACGCGGGCGACTCCGTGACGGCCACCCAGGGGCCGATCACTTAAG11.4285714285714286No Hit
TCGAACACCCGCGCGTGGCCGCCGTCGTGCTCTCCCTGTCCCTGTCTCTT11.4285714285714286No Hit
ATCTCCAAGCCCACGAGACATGATGATATCTCGTATGCCGTCTTCTGCTT11.4285714285714286TruSeq Adapter, Index 25 (96% over 28bp)
CCCCATTACTAAACCCACACTCAACAGAAACAAAGCATACATCATTATCT11.4285714285714286No Hit
CACGTGCGGACCGGGAAGACGCGCAGCATCAGGTCCACGGTCTCCCGGCT11.4285714285714286No Hit
GAGGAGGGGAGGAAGGCTCTGGCCGCAGCTGCTCATCTCAGCAGGTCTTT11.4285714285714286No Hit
AGCCCACGAGACATGATGATATCTCGTATGCCGTCTTCTGCTTGAAAAAA11.4285714285714286TruSeq Adapter, Index 25 (96% over 29bp)
ACAGAGAAAGACAGAGACAGAGAGAGAGAGAGAGAGAGAGAGAGAGAAAC11.4285714285714286No Hit
GACGAACAGGGCGACGAGGATGTCGAACACGGGATGCCTGTCTCTTATAC11.4285714285714286No Hit
CATCACCGGCGTCCTGGGCCTGGCCGCCGTCGTACTCATCCACGAAGTCG11.4285714285714286No Hit
GTCCAGGCCGGGCAGGTGCAGGGAGTCGGCCACGAGGCCGGCCCGGGCGG11.4285714285714286No Hit
CACATCTCCGAGCCCACAAGACGGTAAACCATCTCGTATGCCGTCTTCTG11.4285714285714286Illumina PCR Primer Index 1 (95% over 23bp)
GGCCGTGCAGACGCTCGTCACCGGCGACCGCCTGGACCTGTCTCTTATAC11.4285714285714286No Hit
GGCTCAGAAAAATCCTGCGAAGAAAAAAACTTCTGAGGTAATAAATAGGA11.4285714285714286No Hit
GGCCGCGGCCGTGCTCGGCCGACACCACGTGCTCGCCGGCGAGGTGGTGC11.4285714285714286No Hit
GTCCGCCCCCGGGCTCGCGGCGCGGGTGTACACGGCGGCCGGCGCCGCGG11.4285714285714286No Hit
CTTCACGAAGATCACGAAGCAGCGCTCGCTCGGCCTGACGCCTGTCTCTT11.4285714285714286No Hit
GCCAGCCACCATGAATATTGCACGGTACCATAAATACTTGACCACCTGTA11.4285714285714286No Hit
GCTCCTTCCTGGGCGGCCGAGCGCACGAGGCCCTGTCTCTTATACACATC11.4285714285714286No Hit
CGACTGAGGTGGTCTTGGTGGGGGACCCGTCCCCGTAATCCCTGTCTCTT11.4285714285714286No Hit
CGCCCAGGTCATGCGCGTCATCACGCGGTCCTGGCGCTGTCTCTTATACA11.4285714285714286No Hit
CCTCGGGCACCGCTCCCGGGACCATCCCGCCCCCGGCCCTTCCTCTTCTC11.4285714285714286No Hit
GCCTATAATCACTGCGCCCGCTCATAAGGGGATGGCCATGGCTAGGTTTA11.4285714285714286No Hit
ACTCGATCCGCTCCGAGCCCACGAGACATGATGATATCTCGTATGCCGTC11.4285714285714286No Hit
ACCCAAGGCACCCCTCTGACATCCGGCCTGTCTCTTATACACATCTCCGA11.4285714285714286No Hit
GATCCTGATCGTGCTGGCCATGTACCTCGTGGCCCAGCTGTTCATGTGCT11.4285714285714286No Hit
GTCCCGGCGGTAGCGGGGCCCGTCGCGGCTGATGCCGTCGATGCGCGTGA11.4285714285714286No Hit
TTTCAGGGATGCTGATAGAAGACATGAGACTCCTGGGTCAGACGTAAAAG11.4285714285714286No Hit
TTATTTGGGTAAATGGTTTGGCTAAGGTTGTCTGGTAGTAAGGTGGAGTG11.4285714285714286No Hit
GTCTCTGCTAGTGTGGAGATAAATCATATTATGGCCAAGGGTCATGATGG11.4285714285714286No Hit
CTTATACACATCTCCGAGCCCCGAGACGGCGTCGAATCTCGTATGCCGTC11.4285714285714286No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCTCCC50.044.042
TGCACAG50.044.017
ACAGACC50.044.020
TGCGTCC50.044.05
CGCCTGC50.044.013
CTCCCCA50.044.044
CTCCTCC50.044.041
CCTGCGT50.044.03
CCTGCAC50.044.015
CCGCCTG50.044.012
ACTCCTC50.044.040
ACCCGAA50.044.034
AACTCCT50.044.039
CCCGCGC50.044.027
CTGCGTC50.044.04
CCCGCCT50.044.011
GTCCCCG50.044.08
CCTCCCC50.044.043
CACCCGA50.044.033
CCCCGCG50.044.026