FastQCFastQC Report
Wed 25 May 2016
SRR1780552_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780552_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58884
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGCTC150.002214934469.995752
TTGGAAT150.002214934469.9957528
GGTCACC150.002214934469.9957531
TAGCCCG150.002214934469.995755
AGTTATG150.002214934469.9957537
GTTGGAA207.8687386E-569.9957527
TTCTGAT150.002214934469.9957519
AAAGTGG252.3730911E-455.99659726
GTATACC200.006805442752.7207761
TCCTTGT200.006805442752.7207761
CTTGTAC200.006921130752.4968153
TAATAGT200.006921130752.49681533
AGCCCGT200.006921130752.4968156
TGTATAA200.006921130752.4968152
TTGTACT200.006921130752.4968154
TGAAAGT200.006921130752.49681524
AGTTGGA200.006921130752.49681526
CCTTTCC200.006921130752.49681568
TTGGTAA200.006921130752.49681518
TTGCCGA200.006921130752.49681541