##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780552_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 58884 Sequences flagged as poor quality 0 Sequence length 76 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.917210108008966 34.0 31.0 34.0 12.0 34.0 2 30.879763603016098 34.0 32.0 34.0 21.0 34.0 3 31.036087901637117 34.0 32.0 34.0 21.0 34.0 4 31.533149921880305 34.0 34.0 34.0 23.0 34.0 5 31.444161402078663 34.0 34.0 34.0 23.0 34.0 6 33.86391889137966 38.0 34.0 38.0 22.0 38.0 7 33.37218938930779 37.0 34.0 38.0 21.0 38.0 8 32.057485904490186 37.0 31.0 38.0 11.0 38.0 9 32.921897289586305 37.0 33.0 38.0 21.0 38.0 10-11 33.36453026288975 37.0 34.0 38.0 21.0 38.0 12-13 33.80030228924665 37.0 34.0 38.0 22.0 38.0 14-15 33.42310305006453 37.0 34.0 38.0 21.0 38.0 16-17 33.26111507370423 37.0 34.0 38.0 21.0 38.0 18-19 33.25135011208478 37.0 34.0 38.0 16.5 38.0 20-21 33.33615243529651 37.0 34.0 38.0 21.0 38.0 22-23 33.29521601793356 37.0 34.0 38.0 21.0 38.0 24-25 33.77110929963997 37.5 34.0 38.0 21.5 38.0 26-27 33.86059031315807 38.0 34.0 38.0 21.5 38.0 28-29 33.956898308538825 38.0 34.0 38.0 22.0 38.0 30-31 33.801975069628426 38.0 34.0 38.0 21.0 38.0 32-33 33.57747435636166 37.0 34.0 38.0 21.0 38.0 34-35 33.42604102982135 37.0 34.0 38.0 21.0 38.0 36-37 33.36962502547381 37.0 34.0 38.0 21.0 38.0 38-39 33.90342028394811 37.5 34.0 38.0 22.0 38.0 40-41 33.40317403708987 37.0 34.0 38.0 21.0 38.0 42-43 33.758321445553975 37.0 34.0 38.0 21.5 38.0 44-45 32.797805855580464 37.0 32.0 38.0 16.0 38.0 46-47 33.516872155424224 37.0 34.0 38.0 21.0 38.0 48-49 33.21993240948305 37.0 34.0 38.0 21.0 38.0 50-51 33.17316248896135 37.0 33.5 38.0 21.0 38.0 52-53 33.21870966646287 37.0 34.0 38.0 21.0 38.0 54-55 32.910162353101015 37.0 32.5 38.0 21.0 38.0 56-57 33.00438998709327 37.0 33.0 38.0 16.0 38.0 58-59 32.85630222131648 37.0 32.0 38.0 16.0 38.0 60-61 32.892984511921746 37.0 32.5 38.0 16.0 38.0 62-63 32.96450648733102 37.0 32.0 38.0 19.5 38.0 64-65 32.969287072889074 37.0 32.5 38.0 19.5 38.0 66-67 32.91865362407445 37.0 32.5 38.0 19.0 38.0 68-69 32.537989946335166 37.0 31.0 38.0 15.0 38.0 70-71 32.346248556483935 37.0 31.0 38.0 11.0 38.0 72-73 32.46693499082943 37.0 31.0 38.0 15.0 38.0 74-75 32.07407784797228 37.0 30.5 38.0 11.0 38.0 76 31.252632294001767 36.0 27.0 37.0 11.0 38.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -2.2852819026485633 1101 2 -0.2542342549958967 1101 3 0.34627992463767043 1101 4 -1.102472898704896 1101 5 -0.05738735094725911 1101 6 -0.9145244982049618 1101 7 -0.6687373876436666 1101 8 -0.10321080202920996 1101 9 0.7480508409861883 1101 10-11 -0.04574233739339206 1101 12-13 -0.8738362319668269 1101 14-15 1.1459885567629726 1101 16-17 -1.216184086620963 1101 18-19 1.2840362340836506 1101 20-21 -0.22872531887018255 1101 22-23 0.5320419952255122 1101 24-25 -0.47575584031370255 1101 26-27 -1.0314054074471102 1101 28-29 0.36824156938876484 1101 30-31 -0.5402158509616513 1101 32-33 0.5414394953591781 1101 34-35 0.0025086796422684188 1101 36-37 1.5183414604558223 1101 38-39 0.3163892766089944 1101 40-41 1.1770187921441888 1101 42-43 1.0746497923266318 1101 44-45 1.3576767278786903 1101 46-47 1.1525072295110093 1101 48-49 -0.2592994873996517 1101 50-51 -0.2907281560965487 1101 52-53 0.5006024130780062 1101 54-55 -0.9927617243169564 1101 56-57 0.5103499753709642 1101 58-59 0.5560045982038417 1101 60-61 1.423881071263537 1101 62-63 -0.4923803991044764 1101 64-65 1.2201930977675843 1101 66-67 0.1714458025236283 1101 68-69 1.3994014832720794 1101 70-71 1.0827591329961948 1101 72-73 0.8434665812497144 1101 74-75 -0.19071785351398418 1101 76 -0.3592914857767795 1102 1 0.7703364791752243 1102 2 0.13519722439137638 1102 3 0.12164745830683898 1102 4 0.33358138319086095 1102 5 0.2839306299307083 1102 6 0.46102808738154977 1102 7 0.7249091631942122 1102 8 2.1189543054037117 1102 9 0.9017467390020215 1102 10-11 -0.1485232564845802 1102 12-13 -3.4672874502252853 1102 14-15 -0.7586840041499485 1102 16-17 -2.0344990861069263 1102 18-19 -0.7937369745109777 1102 20-21 -0.6871657424334643 1102 22-23 -0.3833481559002223 1102 24-25 0.19112500681286093 1102 26-27 -5.443788464916011 1102 28-29 -0.22960463190659652 1102 30-31 0.6275131507860578 1102 32-33 -7.100742061133058 1102 34-35 -0.6612879688618989 1102 36-37 1.2221555860855133 1102 38-39 1.0198949823764423 1102 40-41 0.736877947073765 1102 42-43 0.5680958702540551 1102 44-45 -5.752429162940679 1102 46-47 0.3864436950193877 1102 48-49 1.2331699276306693 1102 50-51 -5.75081348559177 1102 52-53 -1.8936609774843518 1102 54-55 0.4171282725988448 1102 56-57 -0.49841428904765905 1102 58-59 1.4270079908223252 1102 60-61 0.5399754477413836 1102 62-63 -4.743588376898245 1102 64-65 -1.2135480351613737 1102 66-67 -0.047121073183163276 1102 68-69 -6.591988462240408 1102 70-71 -0.031998757411940915 1102 72-73 0.6003032215293516 1102 74-75 -6.44791431696623 1102 76 -1.0140668533104495 1103 1 0.08829211719496044 1103 2 -0.8303083841099301 1103 3 -0.07519828682261931 1103 4 -0.14727380783553912 1103 5 0.38454629999902323 1103 6 -2.0943400495839555 1103 7 -0.30713941570433434 1103 8 0.9583740574489141 1103 9 0.4962478775699566 1103 10-11 0.037674769997664725 1103 12-13 -1.335635261644697 1103 14-15 -0.9006043887329014 1103 16-17 -0.19944384447751418 1103 18-19 -0.26544145287819276 1103 20-21 -0.9837626893846263 1103 22-23 -0.4045592548291239 1103 24-25 0.6840194995175821 1103 26-27 -0.7790123832764735 1103 28-29 0.4798286142770891 1103 30-31 -0.42102357857211814 1103 32-33 -4.977355032373676 1103 34-35 -0.6205736229059227 1103 36-37 -0.7621996471100729 1103 38-39 0.6660112006441281 1103 40-41 0.17402478016813916 1103 42-43 0.35558427372879464 1103 44-45 -4.186097038457248 1103 46-47 -0.39351750933246166 1103 48-49 0.1184486659287245 1103 50-51 -0.470325166455396 1103 52-53 -0.37013057350358025 1103 54-55 0.4247608946786272 1103 56-57 0.7560556443250306 1103 58-59 0.2332195114430604 1103 60-61 0.9143745368870384 1103 62-63 0.5433729117919768 1103 64-65 0.6899688746809147 1103 66-67 -0.13824867713019984 1103 68-69 -2.9309232686838627 1103 70-71 0.7877069016923812 1103 72-73 1.5100822550116817 1103 74-75 -0.7274327448991009 1103 76 1.3061659205783904 1104 1 -2.449469858852943 1104 2 -0.6006302403973542 1104 3 0.1729678808420516 1104 4 -0.07478129286547741 1104 5 0.19854330598704806 1104 6 -1.030081067548025 1104 7 0.3233246561519536 1104 8 0.7125282490656772 1104 9 -0.34323565536417533 1104 10-11 -0.7678454395831693 1104 12-13 -1.455975830506972 1104 14-15 -0.9092121731640361 1104 16-17 -0.6282050720224248 1104 18-19 2.7710999606256337E-4 1104 20-21 0.6921916519327382 1104 22-23 -0.3931632967452998 1104 24-25 0.3355352180804587 1104 26-27 0.6037907604360981 1104 28-29 -0.7387155473995506 1104 30-31 -0.7485872013266146 1104 32-33 -0.45276670902038063 1104 34-35 0.13818386212402345 1104 36-37 -0.3058373716609637 1104 38-39 -0.38842367959539104 1104 40-41 0.5020187921441917 1104 42-43 0.18539340546531946 1104 44-45 -3.5287055713913844 1104 46-47 0.018177849948969538 1104 48-49 0.9330818994616621 1104 50-51 1.2772234862392153 1104 52-53 -0.20272787889280153 1104 54-55 0.4918641880918102 1104 56-57 0.17706986588191143 1104 58-59 0.3893759485688051 1104 60-61 0.38658636323434337 1104 62-63 0.10456303155245905 1104 64-65 0.6300699225851076 1104 66-67 0.5453965324506314 1104 68-69 -3.6029479692826634 1104 70-71 -0.7201149545950436 1104 72-73 0.6188771651913214 1104 74-75 -0.7732224155577789 1104 76 -0.054911923732984036 1105 1 -0.7400393854801628 1105 2 -0.7588772818174725 1105 3 -0.494820580696409 1105 4 -0.30551354138619047 1105 5 0.4644456728509532 1105 6 0.20442337032179836 1105 7 1.101357200530657 1105 8 1.012195408828994 1105 9 1.2076889008488365 1105 10-11 1.0873505663339884 1105 12-13 -1.0089159192643677 1105 14-15 0.357429838670285 1105 16-17 1.122368152829651 1105 18-19 0.8220226721262378 1105 20-21 -0.1780191468838339 1105 22-23 1.0450208748523409 1105 24-25 0.48792057311004555 1105 26-27 0.42555481369053894 1105 28-29 -0.5798213166303228 1105 30-31 0.4351221182000131 1105 32-33 0.8756726400920414 1105 34-35 1.315772619520473 1105 36-37 0.8392070070372526 1105 38-39 0.5539394860850777 1105 40-41 1.1503915732092764 1105 42-43 0.9358556835718304 1105 44-45 1.7383617363009236 1105 46-47 1.2525158677004455 1105 48-49 0.746358526680595 1105 50-51 -0.11040595163061084 1105 52-53 -0.8118624942774133 1105 54-55 -0.7400514923815678 1105 56-57 -0.021395370804476954 1105 58-59 -0.20631561947853072 1105 60-61 -0.03288479061180993 1105 62-63 0.2685793037418094 1105 64-65 0.13093900542533987 1105 66-67 0.01325895848614067 1105 68-69 1.1202583916640805 1105 70-71 1.0248184773576128 1105 72-73 0.11632539004339293 1105 74-75 -0.7294169421850007 1105 76 -2.1963320420761825 1106 1 0.4080056313431335 1106 2 0.22522760273989917 1106 3 -0.08556970085729532 1106 4 0.488004654846943 1106 5 0.668723044549143 1106 6 -0.2083081917310352 1106 7 1.1487331528839846 1106 8 1.4964743274970544 1106 9 1.2764130374462823 1106 10-11 0.7516194296978753 1106 12-13 0.3538485158982567 1106 14-15 0.5291048203000202 1106 16-17 1.011643784186731 1106 18-19 0.6530548877738411 1106 20-21 0.4659457369000606 1106 22-23 0.272870199986734 1106 24-25 0.8832680612177128 1106 26-27 0.5085497473642064 1106 28-29 -1.2452310702753735 1106 30-31 -0.4819409594965478 1106 32-33 0.8616532256201381 1106 34-35 1.2735596791174828 1106 36-37 0.8850940008880528 1106 38-39 0.7155591635418617 1106 40-41 0.6672148705755561 1106 42-43 0.8917863793215304 1106 44-45 1.2149831540988103 1106 46-47 1.0655838957006054 1106 48-49 0.6033024876969577 1106 50-51 0.9428484862392139 1106 52-53 0.5192083956170066 1106 54-55 0.9376566717519381 1106 56-57 1.332686695947281 1106 58-59 -0.22776048933969406 1106 60-61 0.7876280299010148 1106 62-63 1.0569731622714116 1106 64-65 1.2901802167027583 1106 66-67 1.843885094542138 1106 68-69 1.379221148364394 1106 70-71 1.1275566140324074 1106 72-73 1.976189256694589 1106 74-75 -0.6728751933355603 1106 76 -0.03726486490945646 1107 1 -0.4057198588529438 1107 2 0.5897661010660578 1107 3 -0.045477241109168176 1107 4 0.4022004144515954 1107 5 0.03848233037729187 1107 6 1.3950713714763623 1107 7 1.0827758756641543 1107 8 0.9514002002851925 1107 9 -0.673571021217839 1107 10-11 -0.47508629324170926 1107 12-13 0.38495404754180385 1107 14-15 -0.30707802682257324 1107 16-17 0.868669927977578 1107 18-19 1.3905210124350873 1107 20-21 1.0466886031522478 1107 22-23 -0.24125780894041782 1107 24-25 0.06183094978778314 1107 26-27 0.7264279555580515 1107 28-29 -0.04229786447272943 1107 30-31 0.49036096940509566 1107 32-33 1.045556461711321 1107 34-35 0.6195863011484164 1107 36-37 0.12678457955854583 1107 38-39 0.6166830277216846 1107 40-41 0.6556773287295528 1107 42-43 0.6049818200994679 1107 44-45 0.5038401603159386 1107 46-47 0.6566687036075081 1107 48-49 0.4024416555592225 1107 50-51 0.7080923886782386 1107 52-53 0.8736440723267123 1107 54-55 0.6200654076040024 1107 56-57 0.5218107195404542 1107 58-59 0.7510375339346567 1107 60-61 0.07827843640507126 1107 62-63 -0.2864430660085162 1107 64-65 0.531213215268032 1107 66-67 1.3958538495238102 1107 68-69 0.9884392258392865 1107 70-71 0.287049679551302 1107 72-73 0.26506618958156025 1107 74-75 0.5927074624909956 1107 76 -0.6610094847085968 1108 1 -0.5623463648770404 1108 2 0.10923421743396844 1108 3 -0.5031616372302388 1108 4 -0.3799770759980099 1108 5 0.5637541493605411 1108 6 0.28972314931944254 1108 7 1.3011861019350874 1108 8 1.0303746346078455 1108 9 0.06482157355148388 1108 10-11 0.3135928134288761 1108 12-13 0.7661250731074816 1108 14-15 1.7617215617757225 1108 16-17 0.355115711110102 1108 18-19 0.760819278670759 1108 20-21 1.0112052061496044 1108 22-23 0.08797375144747832 1108 24-25 0.02932286868287548 1108 26-27 0.040048290556583765 1108 28-29 0.26471065741972666 1108 30-31 1.3419624974685647 1108 32-33 0.5944471464013006 1108 34-35 -0.05955710173140005 1108 36-37 0.303650580146261 1108 38-39 1.2230597541395483 1108 40-41 0.3185850572044302 1108 42-43 0.49695214040507807 1108 44-45 0.2650067780062031 1108 46-47 1.1330498379007778 1108 48-49 0.4117716584978055 1108 50-51 0.0030894500946345715 1108 52-53 0.33063055484213777 1108 54-55 0.7842587664050598 1108 56-57 1.5524087213035997 1108 58-59 -0.18159543697336034 1108 60-61 1.3227685921500054 1108 62-63 0.4347588146849901 1108 64-65 -0.12938037861971452 1108 66-67 -0.10392575670599058 1108 68-69 1.2523607656571016 1108 70-71 2.0314663707061626 1108 72-73 0.9725669242274648 1108 74-75 1.4392399338398043 1108 76 0.3270157871103869 1109 1 -0.4259510727257769 1109 2 -1.5631663675649854 1109 3 0.018741002229333503 1109 4 -0.36824227552443745 1109 5 0.05222827708531241 1109 6 0.2898972561513986 1109 7 -1.352759158876946 1109 8 0.17390830686915137 1109 9 0.14769642556067453 1109 10-11 0.5329674216885039 1109 12-13 0.22012604810574032 1109 14-15 -0.5490243118923601 1109 16-17 -0.9326306500571064 1109 18-19 -0.15362462410798372 1109 20-21 0.6752660738980509 1109 22-23 -0.1332463892308482 1109 24-25 0.7278220677914433 1109 26-27 0.7106729858696283 1109 28-29 0.6402765046235643 1109 30-31 0.15450167150575567 1109 32-33 0.8751595915576544 1109 34-35 0.2102214343783544 1109 36-37 -0.005476099984669247 1109 38-39 0.6843906267629976 1109 40-41 0.23193208694187462 1109 42-43 0.6081715846560769 1109 44-45 -0.30921496445496643 1109 46-47 -0.08982431768108512 1109 48-49 -0.714786597648164 1109 50-51 0.9081664053143612 1109 52-53 0.4133487107025715 1109 54-55 0.15562863895885926 1109 56-57 0.8967049814888597 1109 58-59 0.24857031273065644 1109 60-61 0.4591116522516785 1109 62-63 0.45481592172586716 1109 64-65 0.43604896882788324 1109 66-67 0.0832216769593046 1109 68-69 1.5543316549947903 1109 70-71 0.7513807101448364 1109 72-73 -0.08029190995318913 1109 74-75 2.0499892896445324 1109 76 -0.30520094107402684 1110 1 -0.23905319218627596 1110 2 -0.2797603573564196 1110 3 -0.07421779167257014 1110 4 -0.22613363204676418 1110 5 -0.7382256998609051 1110 6 -0.6850883774895493 1110 7 -0.3125671567135413 1110 8 0.6904887753814677 1110 9 -1.5185646027325994 1110 10-11 -1.606825702741066 1110 12-13 -1.4032894269982066 1110 14-15 -0.19466538953830081 1110 16-17 0.37817285195419004 1110 18-19 0.381125063212437 1110 20-21 -0.5110064474824654 1110 22-23 -0.7527575949909107 1110 24-25 -0.07493627314760687 1110 26-27 -1.4009789764060017 1110 28-29 0.09584220113845277 1110 30-31 0.21568545949210005 1110 32-33 0.9338926477047593 1110 34-35 0.42027450539887923 1110 36-37 -0.0062028687369846125 1110 38-39 0.07899225607712168 1110 40-41 -0.11830284528271306 1110 42-43 -0.03545820272181288 1110 44-45 1.3283302473220644 1110 46-47 0.017940276849550685 1110 48-49 0.6929137708066904 1110 50-51 -0.1565667184391515 1110 52-53 -0.2812732005302294 1110 54-55 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-0.3248149278680117 2116 44-45 1.3873360952752805 2116 46-47 -0.0170304833843673 2116 48-49 0.7134985661283295 2116 50-51 0.4128484862392128 2116 52-53 0.3035221211071999 2116 54-55 -0.3652191452415252 2116 56-57 -0.7918884674514146 2116 58-59 -0.4041657180978646 2116 60-61 -1.459038636765655 2116 62-63 0.04997969821912562 2116 64-65 0.5352782559184348 2116 66-67 -0.5491868008826941 2116 68-69 -0.22273963594933122 2116 70-71 -2.423031621261714 2116 72-73 0.6909604985246531 2116 74-75 -1.5184307488911166 2116 76 -1.4615785903996539 2117 1 -0.19243314556623048 2117 2 -0.4097648557819724 2117 3 0.4702143843385542 2117 4 0.1388113644771778 2117 5 -1.1611594912157486 2117 6 -0.5411387598557198 2117 7 -1.470993525666227 2117 8 -2.2804350376476066 2117 9 0.6864409180623952 2117 10-11 0.6303844555217211 2117 12-13 0.22669462403847973 2117 14-15 -0.7208380472899094 2117 16-17 -0.2912951069874552 2117 18-19 -0.4359117011927509 2117 20-21 -0.7288529284868446 2117 22-23 0.12167498996799253 2117 24-25 0.3077161621364084 2117 26-27 0.7114612149815542 2117 28-29 0.0390598022507902 2117 30-31 0.328882204267785 2117 32-33 1.7153714028677243 2117 34-35 -0.45910634766619296 2117 36-37 -1.0324982108218066 2117 38-39 -0.2516011271478362 2117 40-41 -1.2915126763872777 2117 42-43 -1.422670405723494 2117 44-45 0.4166834146226037 2117 46-47 -0.7862146325685089 2117 48-49 -0.41932194669218603 2117 50-51 0.5827086260993539 2117 52-53 0.9853403029253869 2117 54-55 -1.3148228748452553 2117 56-57 -1.1343593299222796 2117 58-59 -1.3410654849976282 2117 60-61 0.6559263981993766 2117 62-63 -1.0280855698461409 2117 64-65 -0.6651879445477604 2117 66-67 -1.8323569640528596 2117 68-69 -0.8945345077442042 2117 70-71 -0.35119012942021754 2117 72-73 -0.059575632011480195 2117 74-75 -1.230761751222115 2117 76 -1.0779888468099088 2118 1 0.1701422101125729 2118 2 0.1297576906371276 2118 3 0.8149937429110174 2118 4 0.22629629334141654 2118 5 -0.08809462504743593 2118 6 0.6822034152105942 2118 7 1.4832384492554027 2118 8 -4.45579071645156 2118 9 -1.0318132415710757 2118 10-11 -0.49575492234179563 2118 12-13 1.1031836522516514 2118 14-15 -0.8329190697157571 2118 16-17 -0.35977834788449314 2118 18-19 -1.204033234831524 2118 20-21 -2.2898126584120924 2118 22-23 -0.09607277950391335 2118 24-25 0.33607401118391067 2118 26-27 0.8431226569878305 2118 28-29 -0.10714227153748368 2118 30-31 -1.2767122013266174 2118 32-33 0.6000350151175482 2118 34-35 1.074821793158506 2118 36-37 0.4273522835114463 2118 38-39 0.16491683764598974 2118 40-41 -0.43332603544201476 2118 42-43 -0.14941262901743357 2118 44-45 0.430669428608617 2118 46-47 -0.3209385293613707 2118 48-49 -1.0179956867452375 2118 50-51 -0.15370323789871776 2118 52-53 -0.7344089133755602 2118 54-55 0.7825969467124949 2118 56-57 0.8097207279508822 2118 58-59 1.0856043968446656 2118 60-61 -0.36524553331737764 2118 62-63 0.6698647556903907 2118 64-65 -0.40839990500110446 2118 66-67 -2.428956915825225 2118 68-69 0.37151323761388255 2118 70-71 -1.4936063339053902 2118 72-73 -3.0727176623948864 2118 74-75 -0.046821553488815226 2118 76 0.04508807626701383 2119 1 -0.11551006864315383 2119 2 1.163661717644601 2119 3 0.7499346640588342 2119 4 0.4744757001415145 2119 5 0.796882466826208 2119 6 1.8364836177666604 2119 7 -1.540923595596297 2119 8 0.509774752562187 2119 9 -0.14572691410543825 2119 10-11 1.2562585813958478 2119 12-13 0.7826386799825329 2119 14-15 -0.5040548305066892 2119 16-17 -0.011574827267182286 2119 18-19 0.008144242863195927 2119 20-21 -0.599482299116211 2119 22-23 1.0272693955623993 2119 24-25 0.18184203626227458 2119 26-27 0.7499227534430943 2119 28-29 0.7663325295235239 2119 30-31 0.4477633231489051 2119 32-33 -0.34756566006932843 2119 34-35 0.8555789670191274 2119 36-37 -0.17935135767495325 2119 38-39 -0.22362909917580964 2119 40-41 -0.5572469421215445 2119 42-43 -0.8072857903388808 2119 44-45 -2.3175823196431296 2119 46-47 -0.6708300171838957 2119 48-49 -0.6675736949439397 2119 50-51 0.5057855491762808 2119 52-53 -1.2594149418298635 2119 54-55 -1.7728648328872154 2119 56-57 0.9600462644833172 2119 58-59 1.1869065429743983 2119 60-61 0.4531291954021768 2119 62-63 -0.13647717823775452 2119 64-65 0.17047639111657276 2119 66-67 0.5942164625205706 2119 68-69 -0.48544359865329056 2119 70-71 -0.5365048147349043 2119 72-73 -1.7588763313121767 2119 74-75 -0.32167084213120845 2119 76 0.39753562871456793 >>END_MODULE >>Per sequence quality scores pass #Quality Count 12 9.0 13 60.0 14 205.0 15 403.0 16 617.0 17 716.0 18 789.0 19 728.0 20 658.0 21 594.0 22 535.0 23 606.0 24 615.0 25 696.0 26 830.0 27 987.0 28 1245.0 29 1469.0 30 1771.0 31 2292.0 32 2805.0 33 3591.0 34 5050.0 35 7038.0 36 11799.0 37 12776.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 37.351251538777184 22.252427848447546 11.540828887977021 28.85549172479825 2 17.731472046735956 19.207254941919707 34.75816860267645 28.30310440866789 3 17.48182868011684 23.91141906120508 29.799266354187893 28.807485904490182 4 13.025609673255891 20.185449358059916 36.41056993410774 30.378371034576457 5 12.90843013382243 36.28829563209021 35.471435364445355 15.33183886964201 6 27.6577678146865 35.67862237619727 16.768561918347938 19.895047890768293 7 26.795054683785068 30.850485700699682 21.27742680524421 21.07703281027104 8 22.717546362339515 32.783098974254465 19.254806059370967 25.24454860403505 9 22.97398274573738 16.6343998369676 21.35724475239454 39.03437266490048 10-11 24.119455200054343 25.607975001698254 26.28133278989199 23.99123700835541 12-13 23.391753277630595 23.523367977718905 26.096223082671017 26.988655661979482 14-15 21.66632701582773 25.074723184566267 23.590449018409075 29.668500781196926 16-17 22.021262142517493 27.40387881258067 25.69543509272468 24.879423952177163 18-19 21.499048977650975 26.828170640581483 26.118300387201955 25.554479994565586 20-21 20.952211126961483 27.03790503362543 26.28557842537871 25.724305414034372 22-23 23.047856803206304 26.255858976971673 26.108959989131176 24.58732423069085 24-25 21.89983696759731 27.820800217376533 25.768460023096257 24.510902791929894 26-27 20.55991440798859 27.887032130969363 26.874023503838057 24.679029957203994 28-29 22.112118741933294 26.57428163847565 28.0424223897833 23.27117722980776 30-31 21.957577610216696 26.54456219006861 26.816282861218667 24.681577338496027 32-33 20.761157530059098 27.880239114190612 26.694008559201144 24.664594796549146 34-35 21.901535221792 27.454826438421303 26.817981115413353 23.825657224373344 36-37 22.318456626587867 26.917328985802598 26.65494871272332 24.109265674886217 38-39 21.759730996535563 27.08545615107669 27.655220433394472 23.499592418993277 40-41 21.77671353848244 26.046973711025068 27.667108212757284 24.509204537735208 42-43 21.272332042660143 25.84488146185721 27.804666802527002 25.078119692955642 44-45 20.148087765776783 26.013008627131306 28.511989674614497 25.326913932477414 46-47 20.630391957068134 25.816011140547516 28.58331635079139 24.970280551592964 48-49 20.06487331023708 26.203213096936352 29.134399836967596 24.597513755858976 50-51 19.807587799741867 26.450309082263434 28.13412811629645 25.607975001698254 52-53 19.231879627742682 26.58362203654643 28.699646763127507 25.484851572583384 54-55 19.11130357991984 27.012431220705114 27.849670538686233 26.02659466068881 56-57 19.4738808504857 26.59890632429862 27.979586984579853 25.947625840635823 58-59 19.470484342096324 26.12509340398071 27.856463555464984 26.547958698457986 60-61 19.29641328714082 26.256708104069016 28.131580735004412 26.31529787378575 62-63 19.606344677671352 25.953569730317234 27.789382514774815 26.6507030772366 64-65 20.43339447048434 26.12594253107805 27.017525983289183 26.42313701514843 66-67 20.377352082059645 26.766184362475375 26.195570953060255 26.66089260240473 68-69 20.053834657971606 26.252462468582298 26.339922559608723 27.353780313837373 70-71 20.54123361184702 26.168398885945248 26.138679437538208 27.15168806466952 72-73 20.812105155899737 26.28897493376809 25.54768697778684 27.351232932545344 74-75 20.638883228041575 26.82392500509476 25.482304191291355 27.054887575572312 76 21.544052713810203 26.10216697235242 25.251341620813804 27.102438693023572 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.5 8 1.0 9 0.5 10 0.0 11 0.5 12 1.0 13 2.5 14 4.0 15 3.5 16 2.5 17 2.0 18 3.5 19 10.0 20 16.5 21 18.0 22 21.0 23 25.0 24 32.0 25 38.0 26 75.5 27 159.0 28 243.5 29 282.0 30 329.5 31 428.5 32 597.5 33 715.0 34 848.0 35 1131.0 36 1488.5 37 1696.0 38 1898.5 39 2431.0 40 2973.5 41 3304.0 42 3422.0 43 3626.5 44 3998.5 45 4225.5 46 4285.0 47 4292.0 48 4164.5 49 3846.5 50 3663.0 51 3397.0 52 2904.0 53 2476.0 54 2275.0 55 2148.0 56 1781.5 57 1399.5 58 1257.0 59 1077.5 60 801.0 61 616.0 62 528.0 63 478.5 64 375.0 65 295.5 66 244.5 67 219.0 68 184.5 69 141.5 70 116.5 71 100.0 72 82.5 73 55.5 74 36.5 75 27.0 76 19.5 77 13.5 78 9.5 79 4.0 80 6.0 81 7.0 82 5.5 83 5.0 84 4.5 85 2.5 86 0.5 87 0.0 88 1.0 89 1.0 90 0.5 91 1.0 92 1.0 93 1.5 94 1.0 95 0.5 96 1.0 97 0.5 98 1.0 99 7.0 100 12.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.6725086610963928 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 58884.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.590313158073506 #Duplication Level Percentage of deduplicated Percentage of total 1 76.0692863949773 46.090618843828544 2 10.46303043892595 12.67916581753957 3 5.359044789506139 9.74118606072957 4 2.7972419978698357 6.77943074519394 5 1.6088345759291438 4.873989538754161 6 1.0650821234374124 3.872019563888323 7 0.7259375525533942 3.078934854969092 8 0.48769549862660466 2.3639698390055024 9 0.3643702001233253 1.9869574077847971 >10 1.0594764280508997 8.533727328306501 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.025473812920317914 0.0 2 0.0 0.0 0.0 0.025473812920317914 0.0 3 0.0 0.0 0.0 0.032266829699069355 0.0 4 0.0 0.0 0.0 0.03736159228313294 0.0 5 0.0 0.0 0.0 0.03736159228313294 0.0 6 0.0 0.0 0.0 0.03736159228313294 0.0 7 0.0 0.0 0.0 0.03736159228313294 0.0 8 0.0 0.0 0.0 0.03736159228313294 0.0 9 0.0 0.0 0.0 0.05264588003532369 0.0 10 0.0 0.0 0.0 0.06113715100876299 0.0 11 0.0 0.0 0.0 0.06623191359282657 0.0 12 0.0 0.0 0.0 0.0696284219822023 0.0 13 0.0 0.0 0.0 0.07132667617689016 0.0 14 0.0 0.0 0.0 0.07472318456626588 0.0 15 0.0 0.0 0.0 0.08321445553970519 0.0 16 0.0 0.0 0.0 0.08321445553970519 0.0 17 0.0 0.0 0.0 0.08830921812376877 0.0 18 0.0 0.0 0.0 0.1001969974865838 0.0 19 0.0 0.0 0.0 0.10189525168127166 0.0 20 0.0 0.0 0.0 0.10359350587595952 0.0 21 0.0 0.0 0.0 0.10699001426533523 0.0 22 0.0 0.0 0.0 0.11038652265471095 0.0 23 0.0 0.0 0.0 0.11378303104408669 0.0 24 0.0 0.0 0.0 0.12736906460158956 0.0 25 0.0 0.0 0.0 0.13586033557502886 0.0 26 0.0 0.0 0.0 0.1392568439644046 0.0 27 0.0 0.0 0.0 0.17152367366347396 0.0 28 0.0 0.0 0.0 0.19190272399972827 0.0 29 0.0 0.0 0.0 0.21567828272535833 0.0 30 0.0 0.0 0.0 0.23266082467223695 0.0 31 0.0 0.0 0.0 0.2717206711500577 0.0 32 0.0 0.0 0.0 0.3090822634331907 0.0 33 0.0 0.0 0.0 0.33285782215882076 0.0 34 0.0 0.0 0.0 0.38720195638883226 0.0 35 0.0 0.0 0.0 0.4789076829019768 0.0 36 0.0 0.0 0.0 0.6674138985123294 0.0 37 0.0 0.0 0.0 0.9357380612730113 0.0 38 0.0 0.0 0.0 1.3908701854493581 0.0 39 0.0 0.0 0.0 1.818830242510699 0.0 40 0.0 0.0 0.0 2.377555872563005 0.0 41 0.0 0.0 0.0 3.1162964472522248 0.0 42 0.0 0.0 0.0 4.016371170436791 0.0 43 0.0 0.0 0.0 4.894368589090415 0.0 44 0.0 0.0 0.0 5.571972012770871 0.0 45 0.0 0.0 0.0 6.34297941715916 0.0 46 0.0 0.0 0.0 7.047754907954623 0.0 47 0.0 0.0 0.0 7.823857074926975 0.0 48 0.0 0.0 0.0 8.549011616058692 0.0 49 0.0 0.0 0.0 9.092452958358807 0.0 50 0.0 0.0 0.0 9.666462876163305 0.0 51 0.0 0.0 0.0 10.347462808233136 0.0 52 0.0 0.0 0.0 11.10658243325861 0.0 53 0.0 0.0 0.0 11.974390326744107 0.0 54 0.0 0.0 0.0 12.70973439304395 0.0 55 0.0 0.0 0.0 13.317709394742206 0.0 56 0.0 0.0 0.0 14.013993614564228 0.0 57 0.0 0.0 0.0 14.562529719448406 0.0 58 0.0 0.0 0.0 15.184090754704163 0.0 59 0.0 0.0 0.0 15.77508321445554 0.0 60 0.0 0.0 0.0 16.255689151552204 0.0 61 0.0 0.0 0.0 16.82460430677264 0.0 62 0.0 0.0 0.0 17.39521771618776 0.0 63 0.0 0.0 0.0 17.98281366754976 0.0 64 0.0 0.0 0.0 18.660417091230215 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCCGCTC 15 0.0022149344 69.99575 2 TTGGAAT 15 0.0022149344 69.99575 28 GGTCACC 15 0.0022149344 69.99575 31 TAGCCCG 15 0.0022149344 69.99575 5 AGTTATG 15 0.0022149344 69.99575 37 GTTGGAA 20 7.8687386E-5 69.99575 27 TTCTGAT 15 0.0022149344 69.99575 19 AAAGTGG 25 2.3730911E-4 55.996597 26 GTATACC 20 0.0068054427 52.720776 1 TCCTTGT 20 0.0068054427 52.720776 1 CTTGTAC 20 0.0069211307 52.496815 3 TAATAGT 20 0.0069211307 52.496815 33 AGCCCGT 20 0.0069211307 52.496815 6 TGTATAA 20 0.0069211307 52.496815 2 TTGTACT 20 0.0069211307 52.496815 4 TGAAAGT 20 0.0069211307 52.496815 24 AGTTGGA 20 0.0069211307 52.496815 26 CCTTTCC 20 0.0069211307 52.496815 68 TTGGTAA 20 0.0069211307 52.496815 18 TTGCCGA 20 0.0069211307 52.496815 41 >>END_MODULE