FastQCFastQC Report
Wed 25 May 2016
SRR1780552_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780552_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58884
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGT7991.3569051015556008No Hit
ATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGTCTT4710.7998777256979825RNA PCR Primer, Index 24 (95% over 23bp)
CTCTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCC1180.2003939949731676No Hit
ATAAAGAATATTGAGGCGCCATTGGCGTGAAGGTAGCGGATGATTCAGCC870.1477481149378439No Hit
TCTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCG720.12227430201752598No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGATTC150.002214400870.0000140
CGAGTGA150.002214400870.0000164
TAGCGGA150.002214400870.0000134
TTATCAA150.002214400870.0000132
TCTCGAG150.002214400870.0000161
CTAATAT150.002214400870.0000121
CGCCAAC150.002214400870.0000115
GAAGCCG200.006919469752.560
GCTGGAT200.006919469752.53
CGTCTCG200.006919469752.559
GTAGCGG200.006919469752.533
AAGCCGC200.006919469752.561
TGATTCA200.006919469752.541
CTCGAGT200.006919469752.562
TCGAGTG200.006919469752.563
ATTATCA200.006919469752.531
AGCTATC200.006919469752.536
GTAAGGT200.006919469752.519
CCAGTAA200.006919469752.514
CCGCCAA200.006919469752.514
Produced by FastQC (version 0.11.5)