FastQCFastQC Report
Wed 25 May 2016
SRR1780551_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780551_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36
Sequences flagged as poor quality0
Sequence length50
%GC61

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTGCCGCTCACCGCGTGGCGGGGGGGGGGGCCCGCGCTGTTCATCGC12.7777777777777777No Hit
ACTTGGTCATCTCTGCAGGAGATGCTTAGGCCAAAAGTACCATCCGTGGG12.7777777777777777No Hit
TCACGTGGGTTTTCAAAAGAACACCCCCGCTGGGGGGGCGGGGGGGGGCT12.7777777777777777No Hit
CGGCTGCGCCGACGCGGACTGGGCCGACGCGGACCTGACCGGCAGCGTCG12.7777777777777777No Hit
GCGCGGGCGCGGTGTGGACCGACGGGGAAGGGGGCCTCGGCAGCCGCCCC12.7777777777777777No Hit
ATGCTTGACCACCACTTAGGTAGCTTATCTCCAGGAAAATGATAGCTGTT12.7777777777777777No Hit
ACGTTGGCGACGCCCCACTAGCAAGCCGCCTCCTGAAACACCTCCCTTGT12.7777777777777777No Hit
TCTATGTGCTGATCCGGCGCCACCTGCATCGCACTTGAGGGGGCCTTGTG12.7777777777777777No Hit
GTGAAGGACGCGCACCCGGGCATGCAGATCGCCGTCGGCCTGTCTCTTAT12.7777777777777777No Hit
TTTTCGCTTCCCACAGGTCTCCTGTCTCTTATACACATCTGACGCTGCCG12.7777777777777777No Hit
CCGCCACCGCCGGCGAGACGCCCACCGACGAGAACGAGGGCCTGCCGGCG12.7777777777777777No Hit
TAGCGGAATCGGGGGTATGCTGTTCGAATTCATAAGAACAGGGAGGTTAG12.7777777777777777No Hit
AGACCGGGGTCTCCTCGAGCTTGACGCGGACGTAGAGCTGTCTCTTATAC12.7777777777777777No Hit
GCTTAAAACTCAAAGGACCTGGCGGCTGTCTCTTATACACATCTGACGCT12.7777777777777777No Hit
CAGCACGTCCTCGGCGGGGGCGGCCTCCTCCCCGTCTCTTATAAACATCT12.7777777777777777No Hit
CTATATTACGGATCATTTCTCTACTCAGAAACCTGAAACATCGGCATTAT12.7777777777777777No Hit
CTTATACACACCTGACGCGGCCGACGAAACTGGCGGTGTAGAGATCGGGG12.7777777777777777No Hit
GATCGCGGGCGCCACGAGCGCCTCGGGGCGGGCCGCCACGCGCACGCTCC12.7777777777777777No Hit
CACCACGAGGACGATGTCGACCGGCACCCCGGTCTCGGTCTGCATGGTCT12.7777777777777777No Hit
GGGTTTGGATGTGAGTGGCGGTTAGTGCGAGGGCTAAGGGGCGCTTTGGA12.7777777777777777No Hit
CTGCCAGGCTCCACTGGGCCTGGAGGTGCAATCCTACGGCCTTGGAACTG12.7777777777777777No Hit
GTCGTGTAGCGGTGAAAGTGGTTTGGTTTAGACGTCCGGGAATTGCATCT12.7777777777777777No Hit
CTTATACACATCTGACGCTGCCGACGAATCTGGCGGTGTAGATTTCGGTT12.7777777777777777No Hit
AGTATGCAGTGTGGAGAAAACAGGTGTGTGGCAAGTGTAGGAAGGGATGG12.7777777777777777No Hit
GCGTTCGAGCGCCAGGACGACTGGGGCGGCCAGTGGAACCTCTGTCTCTT12.7777777777777777No Hit
GTATTAGCAAACTCATCACTAGACATCGTACTACACGACACGTACTACGT12.7777777777777777No Hit
CGCTGACACCGCGCGGGGCCCTCCTCCTGACCGGAGCGGTCAACACCTGT12.7777777777777777No Hit
GATGTGCGCGTACCGGTGGTTGTCGAAGAGCCGGAAGCTGTCTCTTATAC12.7777777777777777No Hit
CTTAATACACATCTGACGCTGCCGACGAATGACCATGTGGCGATCTCGGT12.7777777777777777No Hit
GGGAGATCATAGACAACTCGGTGGACGAGGCGCGGGCCGGCTTCGGGCAG12.7777777777777777No Hit
TTTTTGACAGGGTGCTGATTGGTGCGTTTACAATCCCTGAGCTAGATACA12.7777777777777777No Hit
GGGGAGATCTCCGCCACGGGCGCGGTGGGCTCCGCGCCGGTCTCGGGGCT12.7777777777777777No Hit
GTCGGGCGCGACGCCGCCGCGCTGGGACGACGCCGTGCGGGACGCCGTGC12.7777777777777777No Hit
CCGCGGACATCGCCCTGATGGGCGACCGGCTCGGGCGCTGTCTCTTATAC12.7777777777777777No Hit
GCGGTGTACCGCTCACCGGTGACGGCCATGCGGGCCCGGATCAGGGTCTT12.7777777777777777No Hit
CAGCGTAGATGCTGTCTCTTATACACATCTGACGCTGCCGACGATCCGTG12.7777777777777777No Hit

[FAIL]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers