FastQCFastQC Report
Wed 25 May 2016
SRR1780551_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780551_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36
Sequences flagged as poor quality0
Sequence length50
%GC62

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTACTTCTAACCTCCCTGTTCTTATGAATTCGAACAGCATACCCCCGA12.7777777777777777No Hit
CGCTCGAGCCGGTCGCCCATCAGGGCGATGTCCGCGGCTGTCTCTTATAC12.7777777777777777No Hit
ATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCCGTCTT12.7777777777777777No Hit
GAGTTGGAGGCCTCGATCACCCGGTAGATGTCGATGCCGTTCGCGCCGGC12.7777777777777777No Hit
CTCTACGTCCGCGTCAAGCTCGAGGAGACCCCGGTCTCTGTCTCTTATAC12.7777777777777777No Hit
GTTGCCTTCCAAGGCCATTCATCCTGTTCTCTTTTTGGGCTCCTGTAGCA12.7777777777777777No Hit
TTCCAAGGCCGTAGGATTGCACCTCCAGGCCCAGTGGAGCCTGGCAGCTG12.7777777777777777No Hit
CATCTACGCTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAA12.7777777777777777No Hit
GGAGGAGGCCGCCACCGCCGAGGACGTGCTGCTGTCTCTTATACACATCT12.7777777777777777No Hit
CTCCTATGATGGCAAATACAGCTCCTATTGATAGGACATAGTGGAAGTGA12.7777777777777777No Hit
GTGCGGGCGGGCAGGTCGCGGACCGCCAGCTCGGAGACGACGCCGGCCAC12.7777777777777777No Hit
ACCATGCAGACCGAGACCGGGGTGCCGGTCGACATCGTCCTCGTGGTGCT12.7777777777777777No Hit
GTGTTGACCGCTCCGGTCAGGAGGAGGGCCCCGCGCGGTGTCAGCGCTGT12.7777777777777777No Hit
GCCGACGGCGATCTGCATGCCCGGGTGCGCGTCCTTCACCTGTCTCTTAT12.7777777777777777No Hit
AAGGTGCAGTCGCCCCGGGAGACCAGGGCGACGCTGCCGGTCAGGTCCGC12.7777777777777777No Hit
GAGACCTGTGGGAAGCGAAAACTGTCTCTTATACACATCTCCGAGCCCAC12.7777777777777777No Hit
CCCCGAGACCGGCGCGGAGCCCACCGCGCCCGTGGCGGAGATCTCCCCCT12.7777777777777777No Hit
GTCTCTTATACACATCTCCGAGCCCACCCAACACGATCTCGTATGCCGTC12.7777777777777777Illumina PCR Primer Index 1 (95% over 22bp)
AGGTTCCACTGGCCGCCCCAGTCGTCCTGGCGCTCGAACGCCTGTCTCTT12.7777777777777777No Hit
GAGCTACCTTAGACCATTGCCAAGGCCAAATTGCGGCAGCTTTCACCCAG12.7777777777777777No Hit
GGGTGATCTCGATCGACTGCCCGAAGCCGGCCAGAGCCTCGTCCACCGAG12.7777777777777777No Hit
CACCAGGGCGATGAACAGCGCGGGCACCCCCCACCGCCACGCGGTGAGCG12.7777777777777777No Hit
TCTTATACAGATCTCCGAGCCACGAGACGGCGTCGAATCTCGTATGCCGT12.7777777777777777No Hit
GTATCTAGCTGCTCTGGTGGGGCCTTGGAGAACCTGTGTGTCAAAACTCT12.7777777777777777No Hit
CAAGACGTCTTGCACTCATGAGCTGTCCCCACATTAGGCTTAAAAACAGA12.7777777777777777No Hit
CTACAAGGCCACCTCAAGTGCGATGCAGGTGGCACCGGATCAGCACAGCG12.7777777777777777No Hit
CCAGAACACTACGAGCCCACGAGACGATCCAAAATCTCGTATGCCGTCTT12.7777777777777777Illumina PCR Primer Index 6 (95% over 22bp)
ATCCTCGCCGGCGCGGCATGGCCGGGAGCGCGCCGGCGAGGATGCACCAT12.7777777777777777No Hit
GTCGAGGAGCGTGCGCGTGGCGGCCCGCCCCGAGGCGCTCGTGGCGCCCG12.7777777777777777No Hit
GGGCCGCCGTGGGCGCGGATCGCGGCGCGGACCCTGTCTCTTATACACAT12.7777777777777777No Hit
GCGAGCGCCCGGGCGGCCTCGGGGTCGAGCCGACCGGGGCGGCGGACGAG12.7777777777777777No Hit
CTTCCGGCTCTTCGACAACCACCGGTACGCGCACATCCTGTCTCTTATAC12.7777777777777777No Hit
GCCCTCGCCGCGGTGCTCGCCCTCGACGCGGCCGGTGGTCCCCCGCTGGG12.7777777777777777No Hit
GAGGTGGTGCTGCGGGTGTCCTCGTGCCAGTGGCGGGGGTCCTCCCTCCT12.7777777777777777No Hit
CCGCCAGGTCCTTTGAGTTTTAAGCCTGTCTCTTATACACATCTCCGAGC12.7777777777777777No Hit
AGCCTATGAAGGCTGTTGCTATAGTTGCAAGCAGGAGGATAATGCCGATG12.7777777777777777No Hit

[FAIL]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers