FastQCFastQC Report
Wed 25 May 2016
SRR1780550_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780550_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences75720
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCTTTC150.002217443469.9953835
AGTCCTG150.002217443469.9953826
AGCCTTT150.002217443469.9953834
TATAGAT207.883525E-569.995385
GGTGTAA252.3775229E-455.99630724
CGCCTTA252.3775229E-455.99630724
GCGACAA200.00692891252.49653241
GGTCCTA200.00692891252.49653228
CCCAACG200.00692891252.49653221
CTACGGG200.00692891252.49653238
TTGGTCC200.00692891252.49653226
GAAAATC200.00692891252.49653256
CAAATAG200.00692891252.49653217
CCAACGT200.00692891252.49653222
CGCAAAG200.00692891252.49653212
CTGGGTT352.0696816E-549.99669656
GCCTTAC305.84638E-446.6635825
TGGTTGG305.84638E-446.6635819
AGCCTCG305.84638E-446.6635810
TAAATGA305.84638E-446.6635828
Produced by FastQC (version 0.11.5)