##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780550_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 75720 Sequences flagged as poor quality 0 Sequence length 76 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.76242736397253 34.0 31.0 34.0 12.0 34.0 2 31.09120443740095 34.0 32.0 34.0 23.0 34.0 3 31.297636027469625 34.0 33.0 34.0 23.0 34.0 4 31.723190702588482 34.0 34.0 34.0 23.0 34.0 5 31.72673005810882 34.0 34.0 34.0 23.0 34.0 6 34.55441098784998 38.0 36.0 38.0 26.0 38.0 7 33.81807976756471 37.0 34.0 38.0 22.0 38.0 8 32.99784733227681 37.0 34.0 38.0 21.0 38.0 9 33.52542260961437 37.0 34.0 38.0 21.0 38.0 10-11 33.88834521922874 37.0 34.0 38.0 22.0 38.0 12-13 34.263913100898044 38.0 34.5 38.0 24.0 38.0 14-15 33.790180929741155 37.0 34.0 38.0 21.5 38.0 16-17 33.641329899630215 37.0 34.0 38.0 21.0 38.0 18-19 33.63083729529846 37.0 34.0 38.0 21.5 38.0 20-21 33.72210776545167 37.0 34.0 38.0 21.5 38.0 22-23 33.70963417855256 37.0 34.0 38.0 21.5 38.0 24-25 34.11805335446381 38.0 34.5 38.0 22.5 38.0 26-27 34.16323956682514 38.0 34.5 38.0 23.0 38.0 28-29 34.3101294241944 38.0 35.0 38.0 23.5 38.0 30-31 34.152350765979925 38.0 34.0 38.0 23.0 38.0 32-33 33.9165412044374 37.5 34.0 38.0 22.0 38.0 34-35 33.74977548864237 37.0 34.0 38.0 22.0 38.0 36-37 33.756695721077655 37.0 34.0 38.0 22.0 38.0 38-39 34.30384970945589 38.0 35.0 38.0 24.0 38.0 40-41 33.763444268357105 37.5 34.0 38.0 21.5 38.0 42-43 34.09481642894876 37.5 34.0 38.0 23.0 38.0 44-45 33.149867934495504 37.0 34.0 38.0 21.0 38.0 46-47 33.887592445853144 37.0 34.0 38.0 23.0 38.0 48-49 33.5816825145272 37.0 34.0 38.0 21.5 38.0 50-51 33.546460644479666 37.0 34.0 38.0 22.0 38.0 52-53 33.56468568409932 37.0 34.0 38.0 21.5 38.0 54-55 33.26164157422082 37.0 33.5 38.0 21.0 38.0 56-57 33.356127839408344 37.0 33.5 38.0 21.5 38.0 58-59 33.23660855784469 37.0 33.0 38.0 21.5 38.0 60-61 33.22761489698891 37.0 33.0 38.0 21.0 38.0 62-63 33.312387744321185 37.0 33.0 38.0 21.0 38.0 64-65 33.31917591125198 37.0 33.0 38.0 21.0 38.0 66-67 33.23489170628632 37.0 33.0 38.0 20.5 38.0 68-69 32.866924194400426 37.0 31.5 38.0 16.0 38.0 70-71 32.7431854199683 37.0 31.5 38.0 19.0 38.0 72-73 32.831246698362385 37.0 32.0 38.0 19.0 38.0 74-75 32.4365557316429 37.0 31.0 38.0 15.0 38.0 76 31.71863444268357 36.0 29.0 38.0 11.0 38.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -3.7565208357884927 1101 2 -1.0769601671001787 1101 3 -0.8575164045383445 1101 4 -3.124652551403969 1101 5 -0.7647714340528893 1101 6 -1.8339570477055034 1101 7 -1.5312192657155066 1101 8 -0.763547742923798 1101 9 -1.7945563819164043 1101 10-11 -1.577197817887786 1101 12-13 -1.788432563054677 1101 14-15 -0.5913929853702342 1101 16-17 -2.053821492048719 1101 18-19 0.1979201262574719 1101 20-21 -0.6984631152825145 1101 22-23 -0.5390758543784244 1101 24-25 -1.8477460344178809 1101 26-27 -2.019444694699594 1101 28-29 -0.2178328388530133 1101 30-31 -1.8330580700958023 1101 32-33 -0.20946192935314656 1101 34-35 -0.8392358355670027 1101 36-37 -0.42225684495983273 1101 38-39 -0.23819577704482953 1101 40-41 0.6554407595871012 1101 42-43 -0.3820640348674402 1101 44-45 -0.24356694564504267 1101 46-47 0.9173976404907407 1101 48-49 -1.279116411492076 1101 50-51 -1.1792552944817913 1101 52-53 0.04848906714335044 1101 54-55 -0.8865883709226665 1101 56-57 -0.899325010399842 1101 58-59 0.2946732190108534 1101 60-61 -0.1302830389485834 1101 62-63 -1.3667666944219121 1101 64-65 -0.3398671801638429 1101 66-67 -0.5017840549678354 1101 68-69 -0.22083573187093464 1101 70-71 -0.4326969179007065 1101 72-73 -0.41713570512711584 1101 74-75 -2.4766645242673704 1101 76 -3.27252907265164 1102 1 1.2208222545576923 1102 2 -0.07788895966983844 1102 3 0.34498852087679666 1102 4 0.5539289290350986 1102 5 0.6137537559274087 1102 6 1.0742613592745158 1102 7 1.43019362479955 1102 8 2.352776490118707 1102 9 1.410340887996341 1102 10-11 0.35263612525885435 1102 12-13 -4.160413987203288 1102 14-15 -0.5354261967409073 1102 16-17 -2.31203989902874 1102 18-19 -0.2907936367597017 1102 20-21 0.3449649736564169 1102 22-23 -0.17275162133591948 1102 24-25 0.3073341794858635 1102 26-27 -5.2467174219723205 1102 28-29 0.5157162745722701 1102 30-31 0.5740626729382541 1102 32-33 -6.703832883476423 1102 34-35 -0.5262045943623832 1102 36-37 0.4181034139762758 1102 38-39 0.9835886304980832 1102 40-41 0.9980526368739007 1102 42-43 0.9346823766158181 1102 44-45 -5.62119148828506 1102 46-47 0.07515165118592648 1102 48-49 0.7626792541425971 1102 50-51 -5.798576994453647 1102 52-53 -1.4308241892596882 1102 54-55 0.6670142184384389 1102 56-57 -0.746651213608402 1102 58-59 1.61498000201113 1102 60-61 0.9386164825825105 1102 62-63 -4.723465259015217 1102 64-65 -0.5303963104762559 1102 66-67 -0.01228222552791891 1102 68-69 -7.664446764800857 1102 70-71 -0.20184974649625786 1102 72-73 0.6752650829393048 1102 74-75 -5.721274712840405 1102 76 -1.1317466352747765 1103 1 -0.41775814138629386 1103 2 -0.9018387423835037 1103 3 -0.23709742794019917 1103 4 0.11211338099067092 1103 5 -0.41994976893980507 1103 6 -1.7247729236805824 1103 7 0.5341047788314199 1103 8 0.4186383925812791 1103 9 0.10734716515713671 1103 10-11 0.6830803550995057 1103 12-13 -1.310558844760024 1103 14-15 -1.2287868920571796 1103 16-17 -1.3625469004773123 1103 18-19 -0.7539515314965399 1103 20-21 -1.024470081872316 1103 22-23 -1.1917521041944426 1103 24-25 0.2637561978344891 1103 26-27 -0.6366256788530507 1103 28-29 0.19287706646024105 1103 30-31 -0.3369706442997966 1103 32-33 -4.801732448903756 1103 34-35 -0.6545966754826225 1103 36-37 -0.09087775454134572 1103 38-39 0.38918978935853943 1103 40-41 0.4902786146624081 1103 42-43 0.16401603185482827 1103 44-45 -5.340216114069705 1103 46-47 0.07515165118592648 1103 48-49 -0.28114015677000737 1103 50-51 0.13449881433437838 1103 52-53 -0.35924041330603984 1103 54-55 -0.3847240722423919 1103 56-57 0.7157341074925156 1103 58-59 0.6059988334935085 1103 60-61 0.5062891045042903 1103 62-63 0.2051682513662456 1103 64-65 0.1896181752794206 1103 66-67 -0.06498623325365571 1103 68-69 -3.16176690000124 1103 70-71 0.25413769918215934 1103 72-73 0.9158010558992302 1103 74-75 -1.3998357944434972 1103 76 0.03971642604825476 1104 1 -2.1291531512465376 1104 2 -0.5984005189418511 1104 3 -0.641238729492116 1104 4 -0.18832389929741922 1104 5 -0.8475841683224701 1104 6 -0.4084735152950856 1104 7 0.35670616783939124 1104 8 1.5070804743784194 1104 9 -0.13392866438929474 1104 10-11 0.0794192044521651 1104 12-13 -0.9991252513449496 1104 14-15 -0.7773593005284098 1104 16-17 -0.6335106319357706 1104 18-19 0.6876372535501929 1104 20-21 0.851826754276189 1104 22-23 0.3250103068490233 1104 24-25 0.7349042729438082 1104 26-27 0.9215068770930941 1104 28-29 -0.4474170259688037 1104 30-31 -0.617280170150778 1104 32-33 -0.29298684314194645 1104 34-35 0.014892306768942376 1104 36-37 0.3558555044829177 1104 38-39 -0.09683438966914792 1104 40-41 0.7225730500563898 1104 42-43 0.8685731783865904 1104 44-45 -2.7820621228153115 1104 46-47 0.2387030530550831 1104 48-49 0.9590639073644383 1104 50-51 1.3252301870515169 1104 52-53 0.005845756633078736 1104 54-55 -0.2082686148129156 1104 56-57 0.6477413097560856 1104 58-59 0.9873115317701107 1104 60-61 0.6749421097345092 1104 62-63 0.9815765511175982 1104 64-65 0.4741290215453873 1104 66-67 0.8348894419585449 1104 68-69 -3.4922136118249583 1104 70-71 -0.562376062285729 1104 72-73 0.76980517147841 1104 74-75 -0.8518207039864976 1104 76 0.048676384892463176 1105 1 -1.294144071209331 1105 2 -0.8798595258958279 1105 3 -0.7067700224008284 1105 4 -0.5870039719377154 1105 5 -0.2654430512564261 1105 6 -0.27238368936604473 1105 7 0.7358309408384613 1105 8 0.7284562107472894 1105 9 0.42555665252615427 1105 10-11 0.49316992421120176 1105 12-13 -1.2240333436512785 1105 14-15 -0.4488834179930947 1105 16-17 0.5907662870525812 1105 18-19 0.6773280893565357 1105 20-21 -0.39272522340021254 1105 22-23 0.31548734099882836 1105 24-25 0.48979863446216854 1105 26-27 -0.23960841723298643 1105 28-29 -1.184682827448185 1105 30-31 -0.7722830491869601 1105 32-33 0.18416911203367903 1105 34-35 0.21198448021979743 1105 36-37 -0.01302654361913369 1105 38-39 -0.38221331512426104 1105 40-41 -0.10868220972125897 1105 42-43 0.387862720841305 1105 44-45 1.3512704723036109 1105 46-47 0.37136018199161214 1105 48-49 0.5551462035065313 1105 50-51 -0.2615727539946775 1105 52-53 -0.8238149600254658 1105 54-55 -1.1331479167573661 1105 56-57 -0.48669860697332723 1105 58-59 -0.7817897660108244 1105 60-61 -0.7026656326581957 1105 62-63 0.3764474374178093 1105 64-65 0.5506338716140462 1105 66-67 0.1832528206182502 1105 68-69 1.1474639361220653 1105 70-71 1.2658229898469742 1105 72-73 -0.11879827450643887 1105 74-75 -1.50418815758972 1105 76 -0.898172177804085 1106 1 -0.12479178053002471 1106 2 0.4231906624624173 1106 3 0.4190775897027095 1106 4 0.2726873635830067 1106 5 0.5572085467506191 1106 6 0.7634651379519752 1106 7 1.3941612361355809 1106 8 1.1728844523319282 1106 9 1.5309886612756998 1106 10-11 0.9738237096043321 1106 12-13 0.5809490357386835 1106 14-15 0.7890677303845948 1106 16-17 1.1564486299861088 1106 18-19 0.28707410952910095 1106 20-21 0.09813636366991574 1106 22-23 0.7255614690824288 1106 24-25 1.0919495641012489 1106 26-27 0.6096928344379364 1106 28-29 -1.02638898858563 1106 30-31 -0.13079562665689082 1106 32-33 1.3367878992496216 1106 34-35 1.598896620212514 1106 36-37 0.7595339133015173 1106 38-39 0.9978936237504499 1106 40-41 0.2977827313408383 1106 42-43 0.9708497180463169 1106 44-45 1.6786735589484039 1106 46-47 1.0238695999038754 1106 48-49 0.20896805306297495 1106 50-51 1.512421656018688 1106 52-53 0.16890590520725368 1106 54-55 0.385165365536956 1106 56-57 1.5366821197249365 1106 58-59 0.421795116720979 1106 60-61 1.4538661452005996 1106 62-63 1.3525806249254089 1106 64-65 1.3496981564603203 1106 66-67 1.3076907839187726 1106 68-69 1.6094398748752567 1106 70-71 1.120957271047601 1106 72-73 1.0682475390796569 1106 74-75 -1.1865918518417544 1106 76 0.17189708942158077 1107 1 -0.7449175666935481 1107 2 0.029310643110697754 1107 3 -0.3749717571768265 1107 4 -0.7343034443512018 1107 5 -0.11698110008053675 1107 6 -0.4816969326817926 1107 7 -0.34299406139707145 1107 8 0.2813266390759992 1107 9 -1.6259053880711818 1107 10-11 -0.662557519229729 1107 12-13 -0.05008628114568836 1107 14-15 -0.4202573784688113 1107 16-17 0.3557108457577556 1107 18-19 0.37104846850346007 1107 20-21 0.48280012757895463 1107 22-23 -0.765427887472967 1107 24-25 -0.5393639337216882 1107 26-27 -0.5533211955572241 1107 28-29 -1.454690875378084 1107 30-31 -0.0927695018383119 1107 32-33 -0.05028273451912213 1107 34-35 0.008874849530357665 1107 36-37 -0.8330882444149879 1107 38-39 0.48110600884962906 1107 40-41 0.44696028334858084 1107 42-43 -0.2562064019343424 1107 44-45 -0.20617162731187477 1107 46-47 -0.14890495258765668 1107 48-49 0.4316216573203491 1107 50-51 0.956313770193816 1107 52-53 0.5709136458942368 1107 54-55 0.4272674458465815 1107 56-57 0.5679714279010355 1107 58-59 0.5194487209783603 1107 60-61 -0.3350429017273129 1107 62-63 -0.5685248419347815 1107 64-65 0.12630676797459017 1107 66-67 0.5652500485535015 1107 68-69 0.6091133146430394 1107 70-71 -0.07888549624799168 1107 72-73 -0.03757007098324294 1107 74-75 -0.1761903037043595 1107 76 -0.7792640136263138 1108 1 -0.19765999758428876 1108 2 0.7107279313950343 1108 3 -0.0027470972382559466 1108 4 0.2924846205895655 1108 5 0.3712812957070888 1108 6 0.49739477420719425 1108 7 1.0016746529513227 1108 8 1.136987016434496 1108 9 0.3667668365887522 1108 10-11 0.10685889147671901 1108 12-13 0.7434415819521618 1108 14-15 0.3686741704561527 1108 16-17 -0.46424308140327497 1108 18-19 0.7065135847825275 1108 20-21 0.7209747655780774 1108 22-23 -0.22923876943873722 1108 24-25 -0.5787123321420466 1108 26-27 -0.3048569568560424 1108 28-29 0.41779705792599486 1108 30-31 0.47296108175098084 1108 32-33 0.8106102009788998 1108 34-35 0.8062311461516884 1108 36-37 0.39203242254421156 1108 38-39 0.6903205766425131 1108 40-41 0.541014693177452 1108 42-43 0.7498598551959859 1108 44-45 0.1781965166109032 1108 46-47 0.8053842093254602 1108 48-49 0.5284075283164071 1108 50-51 0.29083549024647937 1108 52-53 0.20516112285781674 1108 54-55 0.616769420396821 1108 56-57 1.1777673910427566 1108 58-59 -0.6889979661651253 1108 60-61 0.7845161153854505 1108 62-63 0.17488235420387355 1108 64-65 0.8002646442361154 1108 66-67 0.6643395614977834 1108 68-69 1.0148066011722179 1108 70-71 1.7806471935282246 1108 72-73 1.473673895668803 1108 74-75 1.6360080288976562 1108 76 1.3057074650924179 1109 1 -0.17922603129455084 1109 2 -0.750462595594513 1109 3 0.46473743444512294 1109 4 0.20844365622003025 1109 5 -0.020867200132464347 1109 6 -0.018350905331956824 1109 7 -0.8328676982328034 1109 8 0.4690971081776141 1109 9 0.7128517523130995 1109 10-11 0.3688601716132638 1109 12-13 -0.16835700989764035 1109 14-15 0.34919475931896926 1109 16-17 -0.2616294692846495 1109 18-19 0.18182828501721815 1109 20-21 0.6085574410634607 1109 22-23 0.6568717490165596 1109 24-25 0.6421965648069659 1109 26-27 0.5720899174772214 1109 28-29 -0.14886605280581477 1109 30-31 0.369451374048829 1109 32-33 1.088175807976981 1109 34-35 -0.19588107915234332 1109 36-37 0.7715075665595705 1109 38-39 0.24928153247780216 1109 40-41 0.30449879814865 1109 42-43 0.565392178643819 1109 44-45 0.5955637024440534 1109 46-47 -0.5739309176214107 1109 48-49 0.18216259552357883 1109 50-51 1.0014712913986017 1109 52-53 1.0375485775196438 1109 54-55 0.2322484048218314 1109 56-57 0.8739909882264598 1109 58-59 0.2069162646861642 1109 60-61 0.657665251293281 1109 62-63 -0.2948317486337544 1109 64-65 0.1368658817014392 1109 66-67 0.19189450069129776 1109 68-69 1.2808936504574717 1109 70-71 0.34358724046656874 1109 72-73 -0.5429145404310489 1109 74-75 1.3868614532629238 1109 76 -0.6391826565205498 1110 1 0.28750595409780644 1110 2 0.16033280834242447 1110 3 0.07135540897082038 1110 4 0.6418185560151635 1110 5 0.62596632618801 1110 6 0.2706844558509047 1110 7 0.2385226999145189 1110 8 2.1068899290558534 1110 9 -1.1494444977982425 1110 10-11 -0.8390092503160105 1110 12-13 -0.33997453906838615 1110 14-15 0.23148744659072662 1110 16-17 0.5915370043948727 1110 18-19 0.8649805073384087 1110 20-21 -0.46096246630271054 1110 22-23 -0.16863818239878015 1110 24-25 0.21995553870915785 1110 26-27 -1.3268145093509602 1110 28-29 -0.05745694975067295 1110 30-31 0.27002588193160904 1110 32-33 0.9366883224510119 1110 34-35 0.6066262692042983 1110 36-37 0.32564046047602346 1110 38-39 0.5541047265634944 1110 40-41 0.2627690395310296 1110 42-43 0.9471504399781026 1110 44-45 1.6104385577037021 1110 46-47 0.004763301671367515 1110 48-49 0.41891328582374854 1110 50-51 -0.24681409205201277 1110 52-53 0.21787279592171416 1110 54-55 -0.6406250253019792 1110 56-57 0.13004781551781264 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2116 54-55 -0.24614367629840217 2116 56-57 -0.7387564767662909 2116 58-59 -1.392914734830974 2116 60-61 -1.927172991101699 2116 62-63 -0.5813599958573192 2116 64-65 -0.3172384157394106 2116 66-67 -0.6280716992121356 2116 68-69 0.22765849835704444 2116 70-71 -2.751849746496255 2116 72-73 -0.464208601271217 2116 74-75 -1.6133799759982992 2116 76 -0.9001676879063538 2117 1 -0.034290916972167906 2117 2 0.16473443820308376 2117 3 0.32876272322203803 2117 4 -0.4880672531301613 2117 5 -1.019488676578728 2117 6 -1.5587083707500327 2117 7 -2.1097845590019233 2117 8 -1.2132720509230204 2117 9 0.2876247712796811 2117 10-11 0.03076538623512448 2117 12-13 0.6087024567563546 2117 14-15 -0.5692822098306252 2117 16-17 -0.26430332962595315 2117 18-19 -0.2954023086986126 2117 20-21 0.016576645322622596 2117 22-23 0.33503403451354075 2117 24-25 0.14153107067757276 2117 26-27 0.6185675521209433 2117 28-29 0.47775608913458 2117 30-31 -0.083286658940672 2117 32-33 1.0814139122694186 2117 34-35 -0.23844893578589677 2117 36-37 -0.5837236926503948 2117 38-39 -0.08390523369607905 2117 40-41 -1.190712020884483 2117 42-43 -1.1418379397190677 2117 44-45 0.2383121931984391 2117 46-47 -0.31604005865863627 2117 48-49 -0.3807622511750992 2117 50-51 1.0909921356254415 2117 52-53 0.9493230700803679 2117 54-55 -0.426127314149511 2117 56-57 -0.5624543224166914 2117 58-59 -1.0461735294860048 2117 60-61 0.976876640749957 2117 62-63 -0.6442042827403327 2117 64-65 -0.20723023466495505 2117 66-67 -0.3281535099566142 2117 68-69 -0.02172792105938015 2117 70-71 0.5736343004085711 2117 72-73 0.593808851687605 2117 74-75 0.29819624434530567 2117 76 -0.8606258280754311 2118 1 -0.7560842975368267 2118 2 0.04278773205948383 2118 3 -0.013496978586143626 2118 4 0.6702017539545402 2118 5 0.5911436592571597 2118 6 0.33280056443025785 2118 7 0.973910058741545 2118 8 -2.7262782896879543 2118 9 -0.6170490153334072 2118 10-11 -0.6562521669001171 2118 12-13 0.5634581932478397 2118 14-15 -0.19904058986657702 2118 16-17 0.141665793774699 2118 18-19 -0.9605841845577672 2118 20-21 -3.174207958674412 2118 22-23 -0.4456839521629945 2118 24-25 -0.016645412350868583 2118 26-27 0.4905274759868661 2118 28-29 -0.26822572327950667 2118 30-31 -0.61847223576207 2118 32-33 -0.2007448061402286 2118 34-35 1.0098856312015272 2118 36-37 -0.3099846633599199 2118 38-39 -0.19014928572102718 2118 40-41 -0.5267593932012886 2118 42-43 -0.6879545729008356 2118 44-45 0.015382088514080294 2118 46-47 -0.31005243044672426 2118 48-49 -1.40756779204429 2118 50-51 -0.4777928913387157 2118 52-53 -1.2740035662736489 2118 54-55 0.4555212001785023 2118 56-57 0.8303896027181352 2118 58-59 0.7950551899810563 2118 60-61 -1.4273609610265119 2118 62-63 0.38416094936286527 2118 64-65 0.11114504290720362 2118 66-67 -1.9506817958823817 2118 68-69 1.3207036111389954 2118 70-71 -0.9807434092245053 2118 72-73 -1.7887252500359914 2118 74-75 -0.047724766546096475 2118 76 0.2389300564545458 2119 1 0.6589203406820943 2119 2 0.4551253943971467 2119 3 0.5833258414509608 2119 4 0.26505333094896955 2119 5 0.2141724162144918 2119 6 0.6543450913319333 2119 7 -1.2989331694588202 2119 8 0.7324415618890399 2119 9 -0.007123226836192487 2119 10-11 0.3397473692779869 2119 12-13 0.22092572571537517 2119 14-15 0.08006423388110306 2119 16-17 0.003330914368390836 2119 18-19 -0.60338334967836 2119 20-21 -1.7898735430899961 2119 22-23 0.8063452686162265 2119 24-25 -0.6415294568777767 2119 26-27 0.4720325780276795 2119 28-29 0.5736110114143713 2119 30-31 -0.39722919309045324 2119 32-33 0.16503673374845818 2119 34-35 0.7862655013313926 2119 36-37 -0.1786071123395132 2119 38-39 0.13296064006746633 2119 40-41 -0.6903145880064798 2119 42-43 -0.7371776712310734 2119 44-45 -1.755431918907071 2119 46-47 -0.21746198517771376 2119 48-49 -0.6945575879626631 2119 50-51 0.5951431012401329 2119 52-53 -1.615782323230981 2119 54-55 -1.3108864992649103 2119 56-57 0.34198515002795915 2119 58-59 0.44156493024079424 2119 60-61 0.5685805974150497 2119 62-63 0.1673242146689944 2119 64-65 0.027946990959158313 2119 66-67 0.1098469610749504 2119 68-69 -0.8919587265233417 2119 70-71 -0.7243078804675491 2119 72-73 -2.5319418548597383 2119 74-75 -0.47432495207485914 2119 76 0.389904082428572 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 1.0 12 21.0 13 99.0 14 256.0 15 459.0 16 594.0 17 713.0 18 741.0 19 799.0 20 711.0 21 694.0 22 608.0 23 669.0 24 730.0 25 921.0 26 940.0 27 1297.0 28 1457.0 29 1770.0 30 2257.0 31 2776.0 32 3460.0 33 4725.0 34 6379.0 35 9297.0 36 15242.0 37 18104.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 34.54465674737724 23.948568617282966 13.029372265879505 28.477402369460293 2 16.90174326465927 20.02905441098785 39.119123085050184 23.950079239302692 3 16.57686212361331 25.443740095087165 29.203645007923928 28.775752773375597 4 12.414157422081352 20.3486529318542 38.21711568938193 29.020073956682513 5 11.883254094030638 38.172213417855254 35.60486001056524 14.339672477548865 6 26.93740095087163 39.50343370311675 17.799788695192817 15.759376650818806 7 25.297147385103013 29.9432118330692 24.799260433174855 19.960380348652933 8 21.63365029054411 37.18172213417855 19.83623877443212 21.34838880084522 9 22.98468040147913 16.55837295298468 22.694136291600632 37.76281035393555 10-11 24.525884838880085 26.244717379820393 27.04899630216587 22.18040147913365 12-13 23.770470153195987 23.90847860538827 25.94360802958267 26.377443211833068 14-15 21.39395139989435 25.351954569466457 23.83848388800845 29.415610142630744 16-17 22.3567089276281 27.612916006339145 25.70919175911252 24.321183306920233 18-19 21.601954569466457 27.006735340728998 26.364236661384044 25.0270734284205 20-21 21.19255150554675 27.37783940834654 26.300184891706284 25.129424194400425 22-23 22.44651347068146 26.84759640781828 26.295562599049127 24.410327522451137 24-25 22.01333861595351 27.776016904384576 26.14698890649762 24.06365557316429 26-27 20.915874273639727 27.9080824088748 27.265583729529848 23.910459587955625 28-29 21.805335446381406 27.01994189117802 28.261357633386158 22.91336502905441 30-31 21.76241415742208 27.34284204965663 27.36265187533016 23.532091917591124 32-33 20.607501320655043 28.00118858954041 27.20285261489699 24.188457474907555 34-35 21.994189117802428 27.45443740095087 27.37057580559958 23.18079767564712 36-37 22.217379820390914 27.11436872688854 26.580824088748017 24.08742736397253 38-39 21.974379292128894 27.56273111463286 27.25501848917063 23.207871104067618 40-41 21.734680401479135 26.326597992604334 27.66178024300053 24.27694136291601 42-43 21.16679873217116 26.438853671421025 27.971473851030108 24.422873745377707 44-45 20.79569466455362 26.288298996302167 27.993264659271 24.922741679873216 46-47 20.920496566296883 26.192551505546753 28.553222398309565 24.333729529846803 48-49 20.487982039091392 26.79543053354464 28.67934495509773 24.037242472266243 50-51 20.045562599049127 26.5095087163233 28.323428420496565 25.121500264131008 52-53 19.654648705758056 26.478473322768092 28.668779714738513 25.198098256735342 54-55 19.453248811410457 27.02588483888008 28.36436872688854 25.15649762282092 56-57 19.548335974643425 26.692419440042258 28.390781827786583 25.36846275752773 58-59 19.728605388272584 26.978341257263605 27.950343370311675 25.34270998415214 60-61 20.223190702588482 26.02879027997887 28.081088219757 25.666930797675647 62-63 19.54239302694136 26.40187533016376 28.030903328050716 26.02482831484416 64-65 20.40742208135235 26.207739038563126 27.106444796619122 26.2783940834654 66-67 20.585710512414156 26.65081880612784 26.902403592181724 25.86106708927628 68-69 20.012546222926574 26.87863180137348 26.609218172213417 26.499603803486526 70-71 20.92313787638669 26.633650290544107 26.498283148441626 25.944928684627577 72-73 21.09482303222398 26.443475964078182 25.760036978341255 26.701664025356575 74-75 20.805599577390385 26.62242472266244 26.00699947173798 26.56497622820919 76 21.240095087163233 25.719756999471738 26.263866877971477 26.776281035393556 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.5 10 1.0 11 1.0 12 1.0 13 0.5 14 0.5 15 1.0 16 3.0 17 5.0 18 6.5 19 7.5 20 12.5 21 18.0 22 25.5 23 41.5 24 73.0 25 96.0 26 143.5 27 217.5 28 309.0 29 374.0 30 462.5 31 628.5 32 885.5 33 1065.0 34 1272.5 35 1700.0 36 2110.0 37 2300.0 38 2585.5 39 3248.5 40 4045.0 41 4633.5 42 4803.0 43 5020.5 44 5345.0 45 5500.0 46 5548.0 47 5708.5 48 5531.0 49 4960.0 50 4727.0 51 4387.5 52 3604.0 53 2906.0 54 2652.0 55 2524.0 56 2040.5 57 1472.5 58 1260.0 59 1092.0 60 799.5 61 596.0 62 517.0 63 451.0 64 335.0 65 255.5 66 198.5 67 171.0 68 161.5 69 128.5 70 97.0 71 89.0 72 66.0 73 34.5 74 22.0 75 18.0 76 16.0 77 11.0 78 8.0 79 8.0 80 7.5 81 4.0 82 1.5 83 2.0 84 1.5 85 1.5 86 1.5 87 1.0 88 1.5 89 1.5 90 1.5 91 1.0 92 0.0 93 0.5 94 1.0 95 1.0 96 1.0 97 1.5 98 2.0 99 2.5 100 3.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.6774960380348652 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 75720.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.13074484944532 #Duplication Level Percentage of deduplicated Percentage of total 1 74.93112947658402 41.310089804543054 2 9.929332854234039 10.948230322239832 3 5.380285064079531 8.898573692551505 4 2.9536471433704636 6.513470681458003 5 1.827763804048389 5.038298996302166 6 1.2696131273206372 4.199683042789223 7 0.8000958198586656 3.087691494981511 8 0.6012696131273206 2.651875330163761 9 0.3928614205294047 1.9492868462757527 >10 1.9140016768475265 15.402799788695193 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.002641310089804543 0.0 2 0.0 0.0 0.0 0.006603275224511358 0.0 3 0.0 0.0 0.0 0.006603275224511358 0.0 4 0.0 0.0 0.0 0.010565240359218173 0.0 5 0.0 0.0 0.0 0.010565240359218173 0.0 6 0.0 0.0 0.0 0.011885895404120444 0.0 7 0.0 0.0 0.0 0.01584786053882726 0.0 8 0.0 0.0 0.0 0.01716851558372953 0.0 9 0.0 0.0 0.0 0.0277337559429477 0.0 10 0.0 0.0 0.0 0.030375066032752245 0.0 11 0.0 0.0 0.0 0.043581616481774964 0.0 12 0.0 0.0 0.0 0.0462229265715795 0.0 13 0.0 0.0 0.0 0.04754358161648178 0.0 14 0.0 0.0 0.0 0.05150554675118859 0.0 15 0.0 0.0 0.0 0.06075013206550449 0.0 16 0.0 0.0 0.0 0.06207078711040676 0.0 17 0.0 0.0 0.0 0.06735340729001585 0.0 18 0.0 0.0 0.0 0.07527733755942947 0.0 19 0.0 0.0 0.0 0.08584257791864765 0.0 20 0.0 0.0 0.0 0.09772847332276809 0.0 21 0.0 0.0 0.0 0.10169043845747491 0.0 22 0.0 0.0 0.0 0.10829371368198626 0.0 23 0.0 0.0 0.0 0.11357633386159535 0.0 24 0.0 0.0 0.0 0.11753829899630217 0.0 25 0.0 0.0 0.0 0.12017960908610671 0.0 26 0.0 0.0 0.0 0.1254622292657158 0.0 27 0.0 0.0 0.0 0.1399894347596408 0.0 28 0.0 0.0 0.0 0.15583729529846804 0.0 29 0.0 0.0 0.0 0.1769677760169044 0.0 30 0.0 0.0 0.0 0.199418911780243 0.0 31 0.0 0.0 0.0 0.2126254622292657 0.0 32 0.0 0.0 0.0 0.24960380348652933 0.0 33 0.0 0.0 0.0 0.277337559429477 0.0 34 0.0 0.0 0.0 0.3248811410459588 0.0 35 0.0 0.0 0.0 0.41336502905441097 0.0 36 0.0 0.0 0.0 0.554675118858954 0.0 37 0.0 0.0 0.0 0.7989963021658743 0.0 38 0.0 0.0 0.0 1.1291600633914423 0.0 39 0.0 0.0 0.0 1.5504490227152667 0.0 40 0.0 0.0 0.0 2.076069730586371 0.0 41 0.0 0.0 0.0 2.665081880612784 0.0 42 0.0 0.0 0.0 3.3452192287374536 0.0 43 0.0 0.0 0.0 4.088748019017433 0.0 44 0.0 0.0 0.0 4.708135235076598 0.0 45 0.0 0.0 0.0 5.342049656629689 0.0 46 0.0 0.0 0.0 6.007659799260433 0.0 47 0.0 0.0 0.0 6.730058108821976 0.0 48 0.0 0.0 0.0 7.382461701003698 0.0 49 0.0 0.0 0.0 8.030903328050712 0.0 50 0.0 0.0 0.0 8.672741679873218 0.0 51 0.0 0.0 0.0 9.322503961965134 0.0 52 0.0 0.0 0.0 9.998679344955098 0.0 53 0.0 0.0 0.0 10.670892762810354 0.0 54 0.0 0.0 0.0 11.306127839408347 0.0 55 0.0 0.0 0.0 11.856840993132593 0.0 56 0.0 0.0 0.0 12.350765979926043 0.0 57 0.0 0.0 0.0 12.890913893291073 0.0 58 0.0 0.0 0.0 13.456154252509245 0.0 59 0.0 0.0 0.0 14.057052297939778 0.0 60 0.0 0.0 0.0 14.553618594823032 0.0 61 0.0 0.0 0.0 15.113576333861594 0.0 62 0.0 0.0 0.0 15.600898045430533 0.0 63 0.0 0.0 0.0 16.09350237717908 0.0 64 0.0 0.0 0.0 16.70760697305864 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCCTTTC 15 0.0022174434 69.99538 35 AGTCCTG 15 0.0022174434 69.99538 26 AGCCTTT 15 0.0022174434 69.99538 34 TATAGAT 20 7.883525E-5 69.99538 5 GGTGTAA 25 2.3775229E-4 55.996307 24 CGCCTTA 25 2.3775229E-4 55.996307 24 GCGACAA 20 0.006928912 52.496532 41 GGTCCTA 20 0.006928912 52.496532 28 CCCAACG 20 0.006928912 52.496532 21 CTACGGG 20 0.006928912 52.496532 38 TTGGTCC 20 0.006928912 52.496532 26 GAAAATC 20 0.006928912 52.496532 56 CAAATAG 20 0.006928912 52.496532 17 CCAACGT 20 0.006928912 52.496532 22 CGCAAAG 20 0.006928912 52.496532 12 CTGGGTT 35 2.0696816E-5 49.996696 56 GCCTTAC 30 5.84638E-4 46.66358 25 TGGTTGG 30 5.84638E-4 46.66358 19 AGCCTCG 30 5.84638E-4 46.66358 10 TAAATGA 30 5.84638E-4 46.66358 28 >>END_MODULE