FastQCFastQC Report
Wed 25 May 2016
SRR1780550_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780550_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences75720
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGT8051.0631273111463286No Hit
ATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGTCTT4490.5929741151611199TruSeq Adapter, Index 12 (95% over 23bp)
ATAAAGAATATTGAGGCGCCATTGGCGTGAAGGTAGCGGATGATTCAGCC1890.24960380348652933No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCC1320.17432646592709986No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTGCGG150.002216862770.039
GTTTTGC150.002216862770.058
AGGCGCC252.3767477E-455.99999614
TATTGAG252.3767477E-455.9999969
GGCGCCA252.3767477E-455.99999615
CGCCATT252.3767477E-455.99999617
GAGGCGC252.3767477E-455.99999613
ATTTACT200.006927103352.57
GCCCAGT200.006927103352.519
TTGGAAG200.006927103352.56
TGCTTGG200.006927103352.512
TGAAGGT200.006927103352.528
CCCAGTG200.006927103352.520
GCGCCAC200.006927103352.512
GGTCACC200.006927103352.531
GCTTGGT200.006927103352.513
GCCGAGT200.006927103352.558
TTCCAGG200.006927103352.558
TCAGACA200.006927103352.534
GTGACAC200.006927103352.524
Produced by FastQC (version 0.11.5)