FastQCFastQC Report
Wed 25 May 2016
SRR1780549_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780549_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55
Sequences flagged as poor quality0
Sequence length50
%GC60

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGCTGTCTCTTA11.8181818181818181No Hit
CTCCTGCCCTCAGGTGATCCACCGGCCTCGGGCTCCTAAAGTGCTGGGAT11.8181818181818181No Hit
ACTAGTCTCAATCTCCAACACATATGGCCTAGACTACGTACATAACCTAA11.8181818181818181No Hit
GCGCCGCTCTGCTGGCCACCGAGTACGCCAAGGACGGCAAGCTGTCTCTT11.8181818181818181No Hit
CGCCGCCACGAGCCCCGGCGCCCGCCTCGACCCGCCACTGCTGCTCGCAC11.8181818181818181No Hit
CGCCCATGCCGCCGCAGCCGCCGGTGAGGGTGAGGGTGCCCTTGAGCTGT11.8181818181818181No Hit
GTCCACGATGATGAAGTCGGGGGCGATCCGCTCCTCCCAGATGGCCTTGG11.8181818181818181No Hit
GACCGCACCCGTGGTGGCCGCGGCCAGCGCGGCGGCGCTGTCTCTTATAC11.8181818181818181No Hit
GACTACAGGTGTACACCACTACACCTGGCTAATTAAAAAAAATTTTTTTT11.8181818181818181No Hit
GCCCACGATCGCGTAGCGGCACCCCAGCGCGGCCAGGAACGCGCCGGAGA11.8181818181818181No Hit
GCGCGCCGAGCTGCGGGAGGAGATCCGGGAGGAGATCCGGGAGGAGCTGC11.8181818181818181No Hit
ATTAATACACATAGGACGCGGCGGAAACGGGCAGGTGGGAGGGTAACTGT11.8181818181818181No Hit
CTCTTATACACATCTGACGCTGCCGACGAACTTCTAGGTGTAGATCTCGG11.8181818181818181No Hit
GTCAAAGGAGTGATGAGTTACAAACACATCTAAGTGACATAAAACAGTGT11.8181818181818181No Hit
CCTCACGGGCGATCGTGACGCCGTCGTTGGTGATGGTGGGGGCGCCCCAC11.8181818181818181No Hit
GGGCACGTTCACCAGAAAGGTCGCGCGCCAGCCGAGGTCGCCGCCGAGCC11.8181818181818181No Hit
ATTGAAAGCAGGAAATCAAAGTGGCAAAGGTCATATGACTCTTGGGGTTC11.8181818181818181No Hit
TCTTATACACATCTGACGCTGCCGACGAAAATTTGCGTGTAGATTTCGGG11.8181818181818181No Hit
GGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCG11.8181818181818181No Hit
CGCCGAGGGGGAGGACGCGGGCGCCGCGGCCGCGGCCGCCGCGGGCATCC11.8181818181818181No Hit
GTAAGGCGCGATCGACGAAGTTCGATCTCCACTGGCGCCTGCGGATGTCA11.8181818181818181No Hit
CCTTATCCCCATACTAGTTATTATCGCACCCCTAAGCCTACTCATTCAAC11.8181818181818181No Hit
CTAGAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCGTTGGTCA11.8181818181818181No Hit
GCCTGCCTGCCCACGGTGGCCACGGCGGCGAAGCACATGGCCTGTCTCTT11.8181818181818181No Hit
GCGGTCAACTGGTCCTCCGTGAGGGCCGACAGGTCCGCCGGGCGGGCGAT11.8181818181818181No Hit
GTGCATGTTCTCCAGGGACGCCCTGGCGTTCTCGAGGTTGCGCTCGTGCT11.8181818181818181No Hit
CGACGAGGAGGTCCCCACCCATGGCCCAGCCCGAGCCCGTCCGTCTCGTC11.8181818181818181No Hit
AGGGAAAAGTATTCAGTAAAGACACCACTTGAGAATTCTAAACTATTCAT11.8181818181818181No Hit
CAGTTGAGCTCGATCATGACGCCGACGCCGTTCTCGACGGTCGCGGCCAC11.8181818181818181No Hit
GAGTGGGCTGGAGCTGGTCACCAGCGCCTCCCGAACCCCAGCACAGACAC11.8181818181818181No Hit
GTGCATCGTTATAACCAGGTCCGTCTGCCTCCGACCCCCGGGCCACCCAG11.8181818181818181No Hit
GATAGTAATAGGGCAAGGACGCCTCCTAGCTTGTTAGGGACGGATCGGAG11.8181818181818181No Hit
NCCCGGGGAGGAGTCCGATCCGGNNGCCCCCCAGGGCGGCGGCCTGCGCG11.8181818181818181No Hit
CCGGGTTCCACGGGCTTACGACAGGATACAAGATTATTCACCTTCTCACA11.8181818181818181No Hit
TTTTCGCTTCCCACAGGTCTCCTGTCTCTTATACACATCTGACGCTGCCG11.8181818181818181No Hit
CCCGCGTCCCTTTCTCCATAAAATTCTTCTTAGTAGCTATTACCTTCTTA11.8181818181818181No Hit
GTCTACGCCCCGGCGGAGGTCAAGGCCACCACGGACTTCGCTCTTATACA11.8181818181818181No Hit
CAAGCATCCCCATTCCAGTGAGTTCACCCTCTAAATCACCACGATCAAAA11.8181818181818181No Hit
CTTTACACATATGCCGCTGCCACGAAGCTGGCGCTGTTGGTCTCGGTGGG11.8181818181818181No Hit
GCGTCCCACGGCTCCGCCAGCTCCCGCGGCCACAGCCCCTGGACGACCTC11.8181818181818181No Hit
TCCTAGAATTAATTCCCCTAAAAATCTTTGAAATAGGGCCCGTATTTACC11.8181818181818181No Hit
CTGCTCGTCCTCGGCCGTGATGCCGGCGGGGGGGGGGCTCTGTCTCTTTT11.8181818181818181No Hit
GGAATTGAATGGAATGGAAACAGCCCGAGTGGAATGGAACGGAATGGAAT11.8181818181818181No Hit
CATCCGGACGACCTCGACTTCGGGGCCGCCGCCACCCTGGCCGGCTTCAG11.8181818181818181No Hit
CTGTCTCTTATACACATCTGACGCTGCCGACGAACCGTGCCGTATAAATG11.8181818181818181No Hit
GTGAGGGACGAGGCCCCCGTGCGCCGCCTGTCTCTTATACACATCTGACG11.8181818181818181No Hit
CTCGTCGACCGCGCCGCGGTCGCGCCGGCCCTTCTCGACATCCCGGTCCA11.8181818181818181No Hit
GGCCGCCGCACCGAAGACCCCCGCGTCGACCGCCCCCGCGCCGGCCTCGA11.8181818181818181No Hit
CCACCTCCCTGCTCTCCCAGGTCAGCGTGGTCCCTGTCTCTTATACACAT11.8181818181818181No Hit
CTCCGTCATCAATTATTCCGGTTCCTACCCTATTAAAGTTGCCCACAACC11.8181818181818181No Hit
AGCGGGAGCTGAGACTGCCGAGTCACATCGAGGCGTACAGGTCGGTGGTC11.8181818181818181No Hit
CAACGTCAAGGAGTCGCAGGTCGCCTGGTTCTGTCTCTTATACACATCTG11.8181818181818181No Hit
GGCCCGCGCGTGCGTCCCGGCTGCGGTCGGCCTCGCTCGAGGGGTCCCCG11.8181818181818181No Hit
ATATAGGCTGAGCAAGAGGTGGTGAGGTTGATCGGGGTTTATCGATTACA11.8181818181818181No Hit
CGTGAGGGGGCCCAGGGTGATCGGCTGGAGCAGGGGCGAGACCGCGACGG11.8181818181818181No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCGGC50.044.014
AACGCGC50.044.038
CCGGAGA50.044.044
GGCACCC50.044.018
CCCAGCG50.044.023
GCCCACG50.044.01
CCAGCGC50.044.024
CCACGAT50.044.03
TCGCGTA50.044.09
GCGGCCA50.044.029
GCGGCAC50.044.016
GAACGCG50.044.037
CACGATC50.044.04
GCGCGGC50.044.027
GATCGCG50.044.07
AGGAACG50.044.035
CGTAGCG50.044.012
CCCACGA50.044.02
GCCAGGA50.044.032
CACCCCA50.044.020
Produced by FastQC (version 0.11.5)