FastQCFastQC Report
Wed 25 May 2016
SRR1780549_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780549_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55
Sequences flagged as poor quality0
Sequence length50
%GC59

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCACGAGACTAACAACAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA11.8181818181818181RNA PCR Primer, Index 36 (100% over 29bp)
CTTGCCGTCCTTGGCGTACTCGGTGGCCAGCAGAGCGGCACCTGTCTCTT11.8181818181818181No Hit
CTATATACCAATTCGGTTCAGTCTAATCCTTTTTGTAGTCACTCATAGGC11.8181818181818181No Hit
GCGCCGCGCAGGCCGCCGCCCTGGGGCGCGGCCGGATCGGACTCCACCCC11.8181818181818181No Hit
CTCTTATACACATCTCCGAGCCCGAGACTGCTGGGTATCTCGTATGCCGT11.8181818181818181No Hit
CTTCGAGGCCGGCGCGGGGGCGGTCGACGCGGGGGTCTTCGGTGCGGCGG11.8181818181818181No Hit
CGCCGCCGCGCTGGCCGCGGCCACCACGGGTGCGGTCCTGTCTCTTATAC11.8181818181818181No Hit
CCCCTGGACCGAGGCCGACGTGCCCCTGCTGGACGAGGCGGCCGAGCTGC11.8181818181818181No Hit
GTATCCTTGCATGCAACTCTACTTGCATGAGGGACTGAGAAGCATCCGGG11.8181818181818181No Hit
CTCGTGGACATCCAGCAGAAGCAGCTCGACCGGGCGCGCGAGCAGCTCCG11.8181818181818181No Hit
GGGTAATAGAAGTCTCAAGCTTGTAATTATGTTGTTATAAAGATTTAATA11.8181818181818181No Hit
GCATGACATCTTCACCCGTCGCGGTCTCGCCCCTGCTCCAGCCGATCACC11.8181818181818181No Hit
GGTCCTCGAGGTCGTCCAGGGGCTGTGGCCGCGGGAGCTGGCGGAGCCGT11.8181818181818181No Hit
CAATAGCTAAGACCCAAACTGGGATTAGATACCCCACTATGCTTAGCCCT11.8181818181818181No Hit
GAGACCTGTGGGAAGCGAAAACTGTCTCTTATACACATCTCCGAGCCCAC11.8181818181818181No Hit
CCGCGCATCCCGGCCGCGGCCCTTGGTCTTCTTGTTCTTGCGGGGCTGGG11.8181818181818181No Hit
GAAGACGAGAGACCACGGGCGAGGCCGGGACGACGGGGAAGGCGCGAGAA11.8181818181818181No Hit
CGTTAAACATGTGTCACTGGGCAGGCGGTGCCTCTAATACCTGTCTCTTA11.8181818181818181No Hit
ATAGCGGCGTCCCAGATCGCGCCGGCCGCGTTCTCCGACCACTTCTCCCG11.8181818181818181No Hit
GCATTAGCCATATAACTCAAACTGACAACTTGTATGAATAGTTTAGAATT11.8181818181818181No Hit
AGCTCCTACCCCGCCGGCATCACGGCCGAGGACGAGCAGCTGTCTCTTAT11.8181818181818181No Hit
CTCAAGGGCACCCTCACCCTCACCGGCGGCTGCGGCGGCATGGGCGCTGT11.8181818181818181No Hit
GCCGCGCTGAAGCCGGCCAGGGTGGCGGCGGCCCCGAAGTCGAGGTCGTC11.8181818181818181No Hit
CTGCTGTGACATCCGCAGGCGCCAGTGGAGATCGAACTTCGTCGATCGCG11.8181818181818181No Hit
GGTAAATACGGGCCCTATTTCAAAGATTTTTAGGGGAATTAATTCTAGGA11.8181818181818181No Hit
GCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTT11.8181818181818181No Hit
GAGCAGCTCGACCATCGCGTCGACGTCCCCGTCGCAGGCCGCGGCGGCCG11.8181818181818181No Hit
CTCCAGGCCGGCATCGACAAGCTCGCCGACACCGTCAAGGTCACCCTCGG11.8181818181818181No Hit
GCGGCGCACGGGGGCCTCGTCCATCACCTGTCTCTTATACACATCTCCGA11.8181818181818181No Hit
ATTCCGTTCCATTCCATTCCATTCCGTTCCATTCCACTCGGGCTGTTTCC11.8181818181818181No Hit
CTAGCCAACCCCTTAAACACCCCTCCCCACATCAAGCCCGAATGATATTT11.8181818181818181No Hit
AGCCCACGAGACATGATGATATCTCGTATGCCGTCTTCTGCTTGAAAAAA11.8181818181818181TruSeq Adapter, Index 25 (96% over 29bp)
GATTAGTTCTGTGGCTGTGAATGTTATAATTAAGGAGATTTGTAGGGAGA11.8181818181818181No Hit
CCCACGAGACGGTAAACCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA11.8181818181818181Illumina PCR Primer Index 1 (96% over 27bp)
GGTGTGGCTAGGCTAAGCGTTTTGAGCTGCATTGCTGCGTGCTTGATGCT11.8181818181818181No Hit
CTGTCTCTTATACACATCTCCAGCCCACGAGACTAACAACAATCTCGTAT11.8181818181818181No Hit
CTCCTAGGCGACCCAGACAATTATACCCTAGCCAACCCCTTAAACACCCC11.8181818181818181No Hit
GCCCCGCTGCTGCCGCAGATCGCCCGCCCGGCGGACCTGTCGGCCCTCAC11.8181818181818181No Hit
TCGCGTGGCTCGGCGGCGACCTCGGCTGGCGCGCGACCTTTCTGGTGAAC11.8181818181818181No Hit
GCCATGTGCTTCGCCGCCGTGGCCACCGTGGGCAGGCAGGCCTGTCTCTT11.8181818181818181No Hit
AACCCCAAGAGTCATATGACCTTTGCCACTTTGATTTCCTGCTTTCAATC11.8181818181818181No Hit
GCCCATCGTGGAGAGGCTGAACGGGATGCCCGCGGCGGCCGCGGCCGCGG11.8181818181818181No Hit
AACCAGGCGACCTGCGACTCCTTGACGTTGCTGTCTCTTATACACATCTC11.8181818181818181No Hit
GGACCACGCTGACCTGGGAGAGCAGGGAGGTGGCTGTCTCTTATACACAT11.8181818181818181No Hit
CACATCTCCGAGCCCACGAACGATCCAAAATCTCGTATGCCGTCTTCTGC11.8181818181818181RNA PCR Primer, Index 19 (96% over 25bp)
GTTAGAAATTGCTCATAATTTATGGTCTATTTTGAAAATGAAGTCTTTAA11.8181818181818181No Hit
ATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCGTCTT11.8181818181818181TruSeq Adapter, Index 7 (95% over 21bp)
GACGTGCTGGCCATGACCAAGGCCATCTGGGAGGAGCGGATCGCCCCCGA11.8181818181818181No Hit
GTCCGTGATGGCCTTGACCTCCTCCGGGGTGTAGACCTGTCTCTTATACA11.8181818181818181No Hit
ATATAAATTGTAAGCAGCCAACTATCAAAGAATGATATAATTGGTCTGCA11.8181818181818181No Hit
GCCGCACCGCGAGCGCCCGGGCGGCCTCGGGGTCGAGCCTGTCTCTTATA11.8181818181818181No Hit
GTCCCGGCTGGCGGAGGGGGCCACGAACCGGACCCGGCCGGCGTGGGGGC11.8181818181818181No Hit
CCTCTCGCCCGAGGACGCCGGCGCCTACACGGGCGACGTCTCCGGCGCGT11.8181818181818181No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTTACCCTAG11.8181818181818181No Hit
CACCAAGCGCGAGGGCCGCTCCGCCGCCGAGGGCCTCGTGGCCGCGACCG11.8181818181818181No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACACG50.044.025
ACACGGG50.044.027
ACGCCGG50.044.015
CCTACAC50.044.024
ACGTCTC50.044.036
GAGGACG50.044.011
CGCCCGA50.044.06
GCGACGT50.044.033
CGCCTAC50.044.022
AGGACGC50.044.012
GGCGCGT50.044.044
GGCGCCT50.044.020
GCCTACA50.044.023
CTCTCGC50.044.02
GGCGACG50.044.032
TACACGG50.044.026
CGAGGAC50.044.010
CGGGCGA50.044.030
TCCGGCG50.044.041
CGGCGCG50.044.043
Produced by FastQC (version 0.11.5)